Brevibacillus choshinensis X23 and application thereof

A technology of Bacillus brevis, bridge stone, applied in application, bacteria, fungicides, etc., can solve the problem of no reliable products, and achieve the effect of strong inhibitory ability, simple culture conditions and good environmental safety

Inactive Publication Date: 2011-08-17
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] It is of great significance to excavate biocontrol bacterial strains that can effectively control bacterial wilt of Solanaceae crops from soil microbial resources, but there is no reliable product available for use at present

Method used

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  • Brevibacillus choshinensis X23 and application thereof
  • Brevibacillus choshinensis X23 and application thereof
  • Brevibacillus choshinensis X23 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: Screening of Brevibacillus bridgestone

[0034] Soil samples were collected from various places, and different culture media were used for screening. Several strains were screened out, and the inhibition experiments of R. solanacearum and other bacteria were carried out one by one. The strains inhibiting R. solanacearum were obtained, and the strains were identified and toxicological experiments were carried out.

Embodiment 2

[0035] Embodiment 2: The physiological life characteristic of Bacillus subtilis and its preservation

[0036] The screened bacterial strains were deposited in the General Microorganism Center of China Committee for the Collection of Microbial Cultures on January 11, 2011, with a preservation number of CGMCCNo.4534.

[0037] Physiological characteristics of the strain:

[0038] Cell morphology: Gram-positive bacilli (1.14×0.38 μm), producing endospores. Cultural and Physiological Characteristics: Mesophilic, grows at 28°C-30°C. It has good heat resistance and can still survive after being heated at 60°C for 30 minutes. Aerobic or facultative anaerobic bacteria, catalase positive, oxidase negative. Although fermentation metabolism can also be carried out, it is mainly respiratory metabolism. Acids are metabolized from the following carbohydrates: cellobiose, fructose, galactose, D-glucose, glycerol, mannose, maltose, D-ribose, sucrose, trehalose, and D-xylose, and cannot be ...

Embodiment 3

[0039] Example 3 Plate inhibition experiment of Brevibacillus bridgestone X23 strain on No. 3 PO41 of solanaceous crop R. solanacearum

[0040] The Brevibacillus bridgelis X23 strain is obtained by plate antagonistic screening, and the screening method is a conventional method in the art. Heat and melt the solid LB medium for cultivating R. solanacearum bacteria, and add the R. solanacearum liquid cultured by fermentation to a final concentration of 1×10 when it is cooled to 45°C 7cfu / mL. Then pour it into a petri dish with a diameter of 9 cm. After the solid LB medium is completely solidified, punch 4 symmetrical holes on the medium with a puncher. Two symmetrical wells were injected with 20 μL of the culture solution of Brevibacillus dectolithi X23, and the other two symmetrical wells were injected with unused medium for culturing Brevibacillus denconia X23 as a control. The experiment was repeated three times, and the diameter of the inhibition zone was measured after inc...

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Abstract

The invention provides a set of production technology for preventing ralstonia solanacearum from growing and reproducing in solanaceae crops to increase crop yield by aiming at the defects of the prior art for controlling the damages of the ralstonia solanacearum to the crops. Brevibacillus choshinensis X23 has a wide bacteriostatic spectrum and good environmental safety and has antagonism effects to various pathogenic fungi, such as phytophthora parasitica var nicotianae, pyricularia grisea, alternaria alternate, phytophthora infestans and the like, so that the brevibacillus choshinensis X23 has favorable development prospect and application potential.

Description

technical field [0001] The invention relates to the field of plant protection, in particular to a soil-dwelling biological control bacterial strain, in particular to a Brevibacillus bridgestone X23 for efficiently preventing and treating bacterial wilt of Solanaceae crops and an application thereof. Background technique [0002] Bacterial wilt is caused by Ralstonia solanacearum, which is a complex species. The pathogen spreads through the soil, and after infecting the plants, it multiplies in large numbers in the ducts, causing the ducts to be blocked, water transport blocked, and the plants to wilt and die due to lack of water; the pathogen can also secrete exopolysaccharides, enzymes and quorum sensing substances A variety of pathogenic factors disrupt the normal metabolic activities of the host. Bacterial wilt occurs commonly in all Solanaceae crop production areas in my country, and is a common devastating disease in the production of all Solanaceae crops. The annual l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P1/00A01P3/00C12R1/01
Inventor 周清明罗宽黎定军肖启明陈武卢芳国张薇王运生
Owner HUNAN AGRICULTURAL UNIV
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