89 results about "Respiratory metabolism" patented technology

Vectors, host cells, and methods are provided for the production of proteins in yeast. The vectors generally contain a selection gene, a yeast 2 micron sequence, and a polynucleotide encoding a polypeptide, where the polynucleotide is operably linked to promoter, and where the polynucleotide contains one or more yeast-preferred codons. Host cells are cultured under conditions where, after an initial batch phase, oxygen concentration is kept high and glucose feed is regulated so that the yeast cells stay in respiratory metabolism.

The invention provides a filler for sewage treatment, a preparation method of the filler and a sewage treatment method with the filler. The filler is prepared by taking a conductive and porous material as a carrier, and adhering anaerobic digestion bacteria, denitrifying bacteria and anaerobic ammonium oxidation bacteria to form a film on the surface of the carrier, wherein the anaerobic digestion bacteria with low redox potential form a bio-anode; and the denitrifying bacteria and the anaerobic ammonium oxidation bacteria with high redox potential form a bio-cathode, so as to form a bio-electrode couple. The filler provided by the invention couples respiratory metabolism of the anaerobic digestion bacteria, the denitrifying bacteria and the anaerobic ammonium oxidation bacteria, promotes electron and proton transfer through the carrier with porous and conductive capabilities, and can synchronously remove organic pollutants and nitrogen-containing pollutants in the sewage at high efficiency.

The invention discloses a fermentation method for improving the yield of isoleucine. The fermentation method comprises the following steps: culturing microorganisms, fermenting, separating fermentation liquor, carrying out ion exchange on fermentation supernatant, deaminizing with a deamination tower, decolorizing with activated carbon and crystallizing by evaporating. Because the corn steep liquor which serves as a hydrolysis liquor in the culture medium and accounts for 40% of the total weight of the culture medium replaces the hydrochloric acid adopted by the traditional method to regulate the pH value, the corrosion of the hydrochloric acid to the stainless steel tank is avoided. In addition, because biotin and VB1 are used as growth factors in the process, the viscosity of the fermentation liquor can be reduced, the respiratory metabolism of the microorganisms can be promoted, and the down-stream finished product can be extracted easily. By adopting the fermentation method disclosed by the invention, a good fermentation index can be achieved, the yield of the isoleucine which is a metabolite can be improved significantly, the average yield of the isoleucine can be up to 36.9g / L and can be increased by 30% compared with that of the metabolite isoleucine obtained by adopting the original method, the conversion rate of the metabolite isoleucine can be increased by 2-4%, and the fermentation cycle can be shortened by 10 hours.

The invention discloses an intelligent animal respiration metabolism measurement system, which comprises a respiration metabolism compartment, a gas analyzer and a data acquisition controller, wherein the respiration metabolism compartment comprises an intelligent artificial climate compartment, a low-oxygen climate compartment and an ordinary climate compartment; the gas analyzer is connected with the respiration metabolism compartment, and is used for analyzing gas in the respiration metabolism compartment; the data acquisition controller is connected with the gas analyzer, and is used for acquiring climate data of the intelligent artificial climate compartment, and performing regulation and control. According to the system, a system error is effectively eliminated, the requirement of uniforming a technical index and operation is met, and in addition, the system is high in gas testing accuracy and stability, accurate and reliable.

Vectors, host cells, and methods are provided for the production of proteins in yeast. The vectors generally contain a selection gene, a yeast 2 micron sequence, and a polynucleotide encoding a polypeptide, where the polynucleotide is operably linked to promoter, and where the polynucleotide contains one or more yeast-preferred codons. Host cells are cultured under conditions where, after an initial batch phase, oxygen concentration is kept high and glucose feed is regulated so that the yeast cells stay in respiratory metabolism.

The invention relates to a method for determining the emission amount of greenhouse gas from ruminant livestock. A sealed type respiratory metabolism box-gas chromatography combined method is used for determining the emission amount of greenhouse gas from the ruminant livestock. The method comprises the following steps: making a movable sealed type respiratory metabolism box; testing the adaptation of the ruminant livestock; using a method for collecting greenhouse gas samples; detecting the content of CO2, CH4 and N2O in the greenhouse gas samples; calculating the emission amount of greenhouse gas from the ruminant livestock. Through the adoption of the method disclosed by the invention, the emission amount of greenhouse gas from the ruminant livestock can be conveniently and accurately measured, and a greenhouse gas emission list with a raise livestock source can be perfected. At the same time, the method provided by the invention, which is also a method for screening superior feed, provides support for realizing the emission reduction of the greenhouse gas for the ruminant livestock. The method has dual benefits of economy and environmental protection for animal husbandry, has the characteristics of economy, simplicity, easy operation, accurate results and the like, and is suitable for the determination of the emission rate of the gas in the greenhouse for the ruminant livestock.

A multifunctional open type respiratory metabolism testing device belongs to the technical field of animal respiratory metabolism experimental facilities. A respiratory metabolism room of animals including cattle, sheep and birds is built by organically combining an air inlet pump, an air exhaust pump, a respiratory metabolism room, an air valve, a gas float flowmeter, a frequency converter, a data collection controller and a microcomputer. The multifunctional open type respiratory metabolism testing device can perform respiratory metabolism testing for the animals including cattle, sheep and birds simultaneously and respectively. An air inlet sample port and an air outlet sample port can be additionally arranged on a gas analyzer, and the same respiratory metabolism rooms are increased in the same connection mode of all respiratory metabolism rooms D and all parts. The multifunctional open type respiratory metabolism testing device can test respiratory metabolism of the animals including cattle, sheep and birds simultaneously and respectively, improves efficiency and saves cost.

The invention provides dimethylformamide (DMF) degrading bacteria and a bacterial agent produced from the same, which belong to the field of biological high technology. The strain is a Gram staining reaction negative bacteria strain DMF3 and is identified as pseudomonasplecoglossicida. The strain has the main biological characteristic of G<->, an irregular rod-shaped strain body, the size of about 0.5-1.0*1.5-5.0 mu m and no stipe or mycoclena, does not produce spore, is aerobic, undergoes strict respiratory metabolism and can grow by taking DMF as a unique carbon source and nitrogen source so as to be completely mineralized for releasing ammonia nitrogen. The strain can reduce the residual quantity of the DMF with certain concentration in waste water by over 95 percent at the temperature of 30 DEG C under the pH value of 7.0 within 3 days, so that the problem of difficult treatment of the DMF in chemical production waste water is solved, the chemical oxygen demand (COD) of the DMF waste water reaches the standard and the waste water can be discharged.

A bacteriostatic agent made from traditional Chinese medicines takes 15-18 parts of glabrous greenbrier rhizome, 15-18 parts of flavescent sophora root, 145-155 parts of isatis root, 12-15 parts of phellodendron bark , 110-120 parts of dandelion, 5-8 parts of baikal skullcap root, 7-10 parts of gentian root, 75-80 parts of lilac flower, 60-65 parts of Chinese silkvine root bark, 10-12 parts of peppermint oil and 0.3-0.5 part of borneol as raw materials; the glarbrous greenbrier rhizome, the flavescent sophora root, the isatis root, the phellodendron bark , the dandelion, the baikal skullcap root, the gentian, the lilac flower and the Chinese silkvine root bark are soaked in ethanol solution and extracted to obtain filtrate, and the filtrate is blended with the peppermint oil and the borneol and then filled to obtain the bacteriostatic agent. The bacteriostatic agent made from the traditional Chinese medicines is made by selecting natural Chinese medicinal herbs with aromatic scent and aseptic and bacteriostatic effects as the raw materials, and can be used for treating dermatosis by spraying the agent on the surface of the focal skin, which can not hinder the normal respiratory metabolism of skin, and can cause agent to be quickly delivered to the subcutaneous focal part; in addition, the bacteriostatic agent has the advantages of quick curative effect and high bioavailability.

The invention discloses a method for measuring the activity of packing biological membranes of artificial wetlands. The method includes simultaneously monitoring changes of the concentration of common electron acceptors in respiratory metabolism of aerobic microorganisms, anaerobic microorganisms and facultative microorganisms according to characteristics of metabolisms of the aerobic microorganisms, the anaerobic microorganisms and the facultative microorganisms in a packing biological membrane of an artificial wetland so that the degree of the activity of the biological membrane is reflected. In the method, the activity of the biological membrane is reflected by means of measuring the changes of the concentration of dissolved oxygen (DO) content, nitrate (NO3-), sulfate radicals (SO42-) and ferric ions [(Fe(III)], a computation formula is provided, and a theoretical basis is reliable. The method has the advantages that results are accurate and stable, interferences are few, operation is simple and speedy, and the method is technically feasible and economically reasonable.

A method of enhancing biomass yield of a lactic acid bacterial species cell culture, comprising cultivating the cells in a process comprising the steps of providing conditions that results in a reduced glycolytic flux and providing conditions that enable the cells to have, under aerobic conditions, a respiratory metabolism. The increased yield of biomass may be the result of an increased yield of ATP which can be obtained by activating the native ATP synthase activity of the H+-ATPase complex by lowering the ATP / ADP ratio, e.g. by carbon source limitation, and / or by increasing the proton gradient (membrane potential) of the cells, e.g. by enhancing or establishing an electron transport chain which can be achieved by enhancing expression of dehydrogenases or electron transport chain components, by adding to the medium a quinone or porphyrin compound or by enhancing the expression of the H+-ATPase activity.

The invention discloses a preservation transport case for rare fresh flowers, and belongs to the technical field of preservation transport devices. The preservation transport case comprises an airtight case body which can be opened, wherein a shock absorption device is arranged below the case body; a spray device is arranged at an opening of the case body; and the case body is internally provided with a signal collection device, a control device, a humidifying device, a temperature control device and a liquid nitrogen controlled atmosphere device. The preservation transport case further comprises a high voltage static adjustment and control device, wherein the high voltage static adjustment and control device comprises a direct current high-voltage power supply, a positive and negative high voltage filter circuit, a positive and negative ionic discharge electrode, a frequency generation module and a frequency selection module. The preservation transport case is advantageous in that, through a high voltage static field, respiratory metabolism activities of plants are inhibited, and moisture in the plants is retained effectively, so that the rare fresh flowers are kept bright in color and can not wither even after long-distance transport.

The present invention relates to a bacterial strain capable of degrading toluene and its application. It is mesophilic pseudomonads, microcampylobacillus 0.5-1.0 micrometer x 1.5-4 micrometer, has nosetula, also has no sheath, belongs to Gram-negative bacterium strain, aerobic, makes strict respiratory metabolism, uses oxygen as end electronic receptor, and can use toluene as unique carbon source. Said invented biological method for treating organic waste gas containing toluene is characterized by that it utilizes microbe, and uses organic component toluene in the waste gas as energy of lifemotion or other nutrient (for example carbon source), through degradation and conversion, to obtain simple inorganic material (carbon dioxide and water) and cell composition portion.

The invention discloses a device and method for online detecting oxygen and carbon dioxide in a biological fermentation tail gas. The device comprises a water removing device, a gas detecting chamber, a singlechip, a heat radiating fan, a power supply and a PC (Personal Computer) terminal, wherein the gas detecting chamber comprises a gas inlet end and a gas outlet end, the gas inlet end is connected with a gas outlet, the gas detecting chamber is internally provided with an oxygen sensor and a carbon dioxide sensor, the singlechip is connected with the oxygen sensor and the carbon dioxide sensor, the power supply is connected with the oxygen sensor, the carbon dioxide sensor and the heat radiating fan, and the PC terminal is connected with the singlechip. The invention further provides a method for online detecting the oxygen and the carbon dioxide in the biological fermentation tail gas. The device is simple in structure, strong in practicability, low in manufacturing cost, capable of continuously online operating for a long time, and capable of simultaneously completing online detection of contents of oxygen and carbon dioxide; the influence of the water in the tail gas is eliminated, and thus important respiratory metabolism parameters such as CER (Carbon dioxide Evolution Rate), OUR (Oxygen Uptake Rate) and RQ (Respiratory Quotient) in a fermentation process are obtained.

The invention discloses a freshness keeping device for fruits and vegetables in supermarkets, and belongs to the technical field of freshness keeping devices for fruits and vegetables. The freshness keeping device comprises a temperature detection unit, a humidity detection unit, a carbon dioxide concentration detection unit, a control unit, a temperature control unit, a humidity control unit, an ozone generation unit and a high-pressure electrostatic generation unit, wherein the high-pressure electrostatic generation unit comprises a direct-current high-voltage power supply, a positive-negative high-voltage filter circuit, a positive-negative ionic discharge electrode, a frequency producing module and a frequency selection module. According to the device disclosed by the invention, a high-voltage electrostatic field is adopted, so that the respiratory metabolism activity of the vegetables and the fruits is restrained, moisture content in the vegetables and the fruits is effectively maintained, and a freshness keeping effect is achieved.

The invention provides a method and a system for optimizing a temperature control interval of aquatic product waterless keep-alive transportation. Acquiring a respiratory metabolism gas concentrationparameter of an aquatic product in a closed transportation container; obtaining the total length of waterless keep-alive transportation; and inputting the obtained respiratory metabolism gas concentration parameter of the aquatic product and the total transportation duration of the waterless keep-alive into a temperature control interval optimization model to obtain an optimal temperature controlinterval of the waterless keep-alive transportation of the aquatic product. The system comprises: receiving module and processing module. According to the embodiment of the invention, through analyzing the gas concentration parameter in the anhydrous keep-alive transportation process of the aquatic product, the optimal selection of the temperature control interval of the anhydrous keep-alive transportation of the aquatic product is realized, and theoretical support is provided for the optimization and intelligent monitoring of the temperature control interval of the anhydrous keep-alive transportation of the aquatic product.

The invention provides a high-value utilization method of CO2 in biogas. After the pretreated raw material is anaerobically fermented, a mixed gas with CO2 and CH4 as the main components is produced, and the mixed gas is transported to the photobioreactor, and the algae in the photobioreactor use the CO2 in the mixed gas as the fuel needed for growth. Carbon source and energy, while the algae attached to the bioanode in the autotrophic microbial fuel device output the electrons generated by respiratory metabolism to the air cathode through an external circuit, and react under the action of a catalyst to generate current, which can be used for Lighting system power required for photobioreactors. Algae can be further utilized as raw materials for anaerobic fermentation or for extracting high value-added substances. The utilization of biogas through photobioreactor and microbial fuel cell reduces the CO2 content and increases the CH4 content. The invention improves the energy conversion efficiency of the whole system.

The invention relates to the technical field of fresh keeping of pomegranates, and discloses a storage fresh keeping method of pomegranates. The method comprises the following steps that (1) a scientific picking method is adopted so as to improve the quality of selected pomegranates, reduce the damage ratio of pomegranate fruits and do preparation work for storage fresh keeping, after precooling treatment is performed on the picked pomegranates, the respiration of skin cells can be reduced, the content of tannin is controlled to be in a stable range and cannot have tremendous fluctuation along with the lapse of time so that peel is prevented from having brown stains, after precooling treatment, a layer of fresh keeping agents is applied to the skin, on one hand, the growth and the propagation of pathogenic bacteria can be restrained, on the other hand, the content of soluble solid matters in pomegranate seeds can be stabilized, and breath and transpiration can be restrained; and (2) the pomegranates on which the fresh keeping agents are applied are packaged with kraft paper to prevent moisture from evaporating, the packaged pomegranates are placed in a wooden box for storage to maintain the initial flavor of the pomegranates, and mixed gas is led into the wooden box to improve the effect of the fresh keeping agents for reducing the respiratory metabolism of the pomegranates and restrain the formation of hazardous substances such as acetic aldehyde and ethanol.

The invention discloses a multifunctional gas analyzer for livestock, and belongs to the technical field of livestock gas analysis. In the multifunctional gas analyzer provided by the invention, a nitrogen sack is connected with inlets of six electromagnetic valves, and outlets of the six electromagnetic valves are connected with normal open holes of another six electromagnetic valves; one ends of six gas backflow air inlet pipes are connected with inlets of six fermentation tanks and respiratory metabolism chambers, and the other ends of the six gas backflow air inlet pipes are connected with normal closed outlets of the six electromagnetic valves; one ends of six gas backflow air outlet pipes are connected with six fermentation tanks, the respiratory metabolism chambers and livestock room outlets, an output end of a sensor group is connected with a microcomputer, and an air inlet of the sensor group is connected with air outlets of the six electromagnetic valves; an air outlet of the sensor group is respectively connected with air inlets of the six electromagnetic valves through a flowmeter, a minitype air pump and a barometer, and the normal closed air inlets of the six electromagnetic valves are connected. The multifunctional gas analyzer provided by the invention has the advantages that six channels can simultaneously on-line measure rumen liquor invitro fermentation and animal respiratory metabolism, the contents of carbon dioxide, methane and ammonia in a livestock room environment are monitored, and methane and carbon dioxide emission reduction tests are performed.

The invention relates to a soda fruit juice stirring rod assembly and a fruit juice device and stirring device provided with the same. The stirring rod assembly (100) comprises a stirring rod shell and a carbon dioxide storage device or carbon dioxide generating device, wherein the carbon dioxide storage device or carbon dioxide generating device is arranged in the stirring rod shell and used for introducing carbon dioxide into fruit and vegetable juice. Part of the carbon dioxide is blended into the fruit juice, and a remaining space of a fruit juice glass is filled with the other part of the carbon dioxide, so that oxygen is expelled; and the carbon dioxide can affect oxygen utilization of aerobic microorganisms, terminate respiratory metabolism of the microorganisms, inhibit growth of the microorganisms and thus prevent the fruit and vegetable juice from being oxidized or layered or discolored, and the fruit and vegetable juice can be preserved for a long time easily. When the carbon dioxide is blended into the fruit and vegetable juice, the effect of forming soda fruit juice can further be achieved, and mouthfeel of the fruit and vegetable juice is enhanced.

The invention discloses a novel application of N-(2-phenylethyl)-5-phenyl-pyridine-2-formamide. The novel application of the medicine provided by the invention is the application of N-(2-phenylethyl)-5-phenyl-pyridine-2-formamide in preparing the medicine for treating / preventing lung injury of mammals. Research finds that N-(2-phenylethyl)-5-phenyl-pyridine-2-formamide has a certain prevention and treatment effect on rat ALI caused by LPS and has an effect of inhibiting inflammatory reaction on RAW264.7 macrophages stimulated by LPS, and the action mechanism can regulate expression of IL-6 and IL-1beta, and is also related to inhibition of hypoxia inducible factor HIF-1alpha by N-(2-phenylethyl)-5-phenyl-pyridine-2-formamide and glycolysis and activation of an acid-sensitive ion channel ASIC1a; researches show that N-(2-phenylethyl)-5-phenyl-pyridine-2-formyl can relieve lung tissue injury caused by LPS, improve respiratory metabolism functions of rats with lung injury, improve pulmonary edema of the rats with lung injury, relieve inflammatory response and inhibit inflammatory diseases related to ASIC1a.

The invention discloses a method for screening functional feed additives for piglets. The method for screening the functional feed additives for the piglets comprises the following steps: culturing porcine intestinal cells IPEC-1 in vitro; adding candidate nutrients from a culture medium of the porcine intestinal cells IPEC-1, and carrying out real-time observation on cell growth state and cell mitochondrial respiratory function indexes; screening the functional feed additives for the piglets from the candidate nutrients according to the cell growth state and the cell mitochondrial respiratoryfunction indexes. The invention aims at providing the method for screening the functional feed additives for the piglets by carrying out cell monitoring in real time and combining a cellular mitochondrial respiratory metabolism function as the assessment index.

The invention relates to a respiratory metabolism device of a cow, belonging to the technical field of experiment equipment. The respiratory metabolism device of the cow mainly comprises a cuboid-shaped respiratory metabolism cabin, air inlet pipes, an air exhaust pipe, an entrance and exit for the tested cow, and a feces discharge pipe, wherein the air inlet pipes and the air exhaust pipe are each connected with an axial flow fan, an air velocity transducer and a gas flowmeter; the entrance and exit for the tested cow to go in and out is formed at the top of the respiratory metabolism cabin, an annular water tank is fixed adjacent to the entrance and exit for the tested cow in a surrounding manner, and the entrance and exit for the tested cow is sealed by a sealing cover; the floor of the respiratory metabolism cabin inclines with certain gradient along the horizontal direction, and the lowest point of the inclined part is connected with a U-shaped feces collection pipe. The respiratory metabolism device can test greenhouse gases generated when the cow has metabolism, is reasonable in integral structural design and simple in structure, the annular water tank at the entrance and exit is novel in structure, the sealing property is good, the U-shaped feces collection pipe fixed at the bottom of the device can be used for guaranteeing the sealing property of the respiratory metabolism cabin while being used for collecting feces and urine of the cow, and thus the accuracy of the test result of the experiment is improved.

The invention relates to a medical intelligent breathing device, and belongs to the technical field of medical instruments. The medical intelligent breathing device comprises a device body, an air purification device and a turbine pump device, wherein an information display screen is arranged on the front side of the device body, a pressure adjusting button, a power key and a main control key are arranged on the lower side of the information display screen, and a pressure gauge is arranged on the right side of the main control key; a wire opening is formed in the device body, a power line is arranged in the wire opening, the power line is connected with a plug, and a breathing hose is arranged on the right side of the device body; the breathing hose is connected with a breathing mask, and a ventilation opening is formed in the breathing mask; elastic belt fixing clamps are arranged on the front side and the rear side of the breathing mask. The breathing mask is complete in function and convenient to use, and when oxygen is supplied to a patient for breathing, the breathing frequency can be well controlled, the purity and humidity of the oxygen can be guaranteed, respiratory metabolism of the patient can be facilitated, and the device is convenient to operate, saves time and the labor, is safe, efficient and comprehensive in function and greatly reduces the working difficulty of medical staff.

The invention discloses a fruit and vegetable preservative and a preparation method thereof. The fruit and vegetable preservative is prepared from 10 to 30 parts of horseradish tree seeds, 10 to 100 parts of an ethanol solution with the mass percent of 45 percent to 60 percent, 1 to 10 parts of potassium sorbate, 1 to 10 parts of citric acid, 0.5 to 5 parts of benzoic acid and 10 to 800 parts of water. The fruit and vegetable preservative is prepared by combining ultrasonic treatment; active ingredients of the horseradish tree seeds are further dissolved out through an ultrasonic effect and a horseradish tree seed oil extract containing multiple active ingredients is obtained; the active ingredients of natural plants can increase water-retaining properties of the fruits and vegetables; active antibacterial ingredients have bacterium-resisting and antibacterial effects; natural film-forming substances inhibit the respiratory metabolism effect of the fruits and vegetables, and the freshness-keeping period and shelf life of the fruits and vegetables are prolonged. Compared with an existing chemical preservative, the fruit and vegetable preservative is safe and toxin-free, is simple in preparation method and has an excellent freshness-keeping effect.

The invention discloses a respiration assistance thoracic cavity saccule counterpulsation drainage device and a respiration assistance drainage method. The drainage device comprises a drainage tube, wherein the drainage tube is communicated with a liquid collector; an air bag is formed on the drainage tube, and is connected with one end of an air inlet tube; the other end of the air inlet tube is connected with a pressure controller; a drainage inlet is formed in the end, near the air bag, on the drainage tube; a pressure sensor is formed on the outer wall of the drainage tube arranged at the drainage inlet; and the pressure sensor is electrically connected with a pressure controller through a transmission wire. The drainage device and the drainage method have the advantages that the pressure in the thoracic cavity is detected through the pressure sensor arranged on the drainage tube; the pressure controller is controlled to generate positive pressure or negative pressure according to requirements; the pressure in the thoracic cavity is changed by the air bag for accelerating the discharging of gas and liquid from the thoracic cavity; the effect of a respiration pump is achieved; the effects of a simple respirator can be partially achieved but the relevant side effects of the respirator are avoided; the burden of the cardio-pulmonary function of a patient can be reduced; and the requirements of respiratory metabolism are met.

The invention discloses a composite chitosan preservative film for citrus gonggan and a preparation method of the composite chitosan preservative film, and belongs to the technical field of food preservation. The composite chitosan preservative film comprises the following components: 0.055-0.15% of chitosan, 0.01-0.65% of sodium alginate, 1.0-4.3% of an acetic acid solution, 0.03-0.50% of glycerin, 0.0001-0.003% of silica sol, 0.050-0.075% of a plant extract, 0.0001-0.001% of sodium trimetaphosphate, and the balance of deionized water supplemented to reach 100%. The composite chitosan preservative film has an antibacterial property, reduces the respiratory metabolism intensity of citrus gonggan, slows down the ripening of fruits and vegetables, and delays the preservation effect and timeof citrus gonggan.