Bacterial strain for degrading toluene and use thereof
A technology of toluene and bacterial strains, which is applied in the field of bacterial strains for degrading toluene and its application, and can solve the problems of complex operation, increased process complexity, equipment investment, secondary pollution, etc.
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Embodiment 1
[0015] This strain has the property of degrading toluene and using them as the sole carbon source. The cedar bark is used as the filler of the biological packing tower. The preserved strain is activated at room temperature for 4 hours and then inoculated into a shake flask containing 50 mL of medium , Under the condition of 30 ℃, the shaker speed is 150 rpm, shake culture for 48 hours, after fully mixing with the sterilized cedar bark filler, fix the bacteria on the surface of the filler (φ150×450mm) in the biological filler tower or In the fiber pores, the column is then hung on the column for aeration and cultured for a week. The biological packing tower made in this way can be used to purify toluene waste gas. When the temperature is 10℃~50℃, the relative humidity is 50%~80%, the inlet toluene concentration is 500mg / m 3 ~4500mg / m 3 , Air flow is 0.3m 3 / h~0.7m 3 Under the operating conditions of / h, the average degradation rate of toluene waste gas is 91.5%. The apparent size o...
Embodiment 2
[0017] The culture of the bacterial species is the same as in Example 1. In this example, sycamore branches are used as the filler, and the apparent size is 10-13mm×10-15mm×5-8mm. The bacteria are fixed on the filler in the biological filler tower (φ150×450mm), Then hang the membrane and culture for 10 days. When the temperature is 10℃~50℃, the relative humidity is 50%~80%, the inlet toluene concentration is 500mg / m 3 ~4500mg / m 3 , Air flow is 0.3m 3 / h~0.7m 3 Under the operating conditions of / h, the average degradation rate of toluene waste gas is 89.7%. Because it is not as loose as cedar bark, the growth rate of biofilm on the surface is slightly lower than that of cedar bark, and the toluene purification treatment rate is also slightly reduced. However, the strength of sycamore wood blocks is much greater than that of cedar bark, so sycamore branches are especially suitable for high-strength packing and packing towers with large handling capacity.
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