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Bacterial strain for degrading toluene and use thereof

A technology of toluene and bacterial strains, which is applied in the field of bacterial strains for degrading toluene and its application, and can solve the problems of complex operation, increased process complexity, equipment investment, secondary pollution, etc.

Inactive Publication Date: 2003-10-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, my country only adopts purification treatment measures for high-concentration industrial organic waste gas, such as absorption method, adsorption method and catalytic combustion method. Although these methods have many advantages, they also have great shortcomings: absorption method and The adsorption method generally needs to regenerate the absorbent and the adsorbent, which not only increases the complexity of the process and the investment of the equipment, but also complicates the operation; the catalytic combustion method will produce harmful gas due to incomplete combustion Combustion products, if the combustion temperature is too high, it will produce NOx pollution
In addition, there are certain safety hazards and high energy consumption problems when the combustion equipment is running.
The most important thing is that the several methods introduced above all have a common disadvantage, that is, the treatment effect on low-concentration organic waste gas is not good, the energy consumption is large, and secondary pollution may occur
Although the concentration of organic matter is low (3 ), and has no recycling value, the purification treatment is difficult and expensive, but the harm to human health and the ecological environment cannot be ignored

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] This strain has the property of degrading toluene and using them as the sole carbon source. The cedar bark is used as the filler of the biological packing tower. The preserved strain is activated at room temperature for 4 hours and then inoculated into a shake flask containing 50 mL of medium , Under the condition of 30 ℃, the shaker speed is 150 rpm, shake culture for 48 hours, after fully mixing with the sterilized cedar bark filler, fix the bacteria on the surface of the filler (φ150×450mm) in the biological filler tower or In the fiber pores, the column is then hung on the column for aeration and cultured for a week. The biological packing tower made in this way can be used to purify toluene waste gas. When the temperature is 10℃~50℃, the relative humidity is 50%~80%, the inlet toluene concentration is 500mg / m 3 ~4500mg / m 3 , Air flow is 0.3m 3 / h~0.7m 3 Under the operating conditions of / h, the average degradation rate of toluene waste gas is 91.5%. The apparent size o...

Embodiment 2

[0017] The culture of the bacterial species is the same as in Example 1. In this example, sycamore branches are used as the filler, and the apparent size is 10-13mm×10-15mm×5-8mm. The bacteria are fixed on the filler in the biological filler tower (φ150×450mm), Then hang the membrane and culture for 10 days. When the temperature is 10℃~50℃, the relative humidity is 50%~80%, the inlet toluene concentration is 500mg / m 3 ~4500mg / m 3 , Air flow is 0.3m 3 / h~0.7m 3 Under the operating conditions of / h, the average degradation rate of toluene waste gas is 89.7%. Because it is not as loose as cedar bark, the growth rate of biofilm on the surface is slightly lower than that of cedar bark, and the toluene purification treatment rate is also slightly reduced. However, the strength of sycamore wood blocks is much greater than that of cedar bark, so sycamore branches are especially suitable for high-strength packing and packing towers with large handling capacity.

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PUM

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Abstract

The present invention relates to a bacterial strain capable of degrading toluene and its application. It is mesophilic pseudomonads, microcampylobacillus 0.5-1.0 micrometer x 1.5-4 micrometer, has nosetula, also has no sheath, belongs to Gram-negative bacterium strain, aerobic, makes strict respiratory metabolism, uses oxygen as end electronic receptor, and can use toluene as unique carbon source. Said invented biological method for treating organic waste gas containing toluene is characterized by that it utilizes microbe, and uses organic component toluene in the waste gas as energy of lifemotion or other nutrient (for example carbon source), through degradation and conversion, to obtain simple inorganic material (carbon dioxide and water) and cell composition portion.

Description

Technical field [0001] The invention relates to a bacterial strain that degrades toluene and its use. Background technique [0002] Volatile organic compounds are a large category of air pollutants second only to particulate pollutants. After entering the atmosphere, these compounds diffuse into the underground aquifer, soil and sediments, causing serious pollution, great harm, and difficult to control. With the rapid development of the organic synthesis industry and petrochemical industry, more and more volatile organic compounds are used, which not only have a stimulating effect on human senses, but many of them also have acute harmful effects on the human central nervous system. And the environment bring great harm. In recent years, the amount of volatile organic compounds, especially toluene, has been increasing. The volatile toluene gas is aromatic and has strong toxicity; it reduces the body’s hematopoietic function by damaging the human liver and...

Claims

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Application Information

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IPC IPC(8): B01D53/46B01D53/84C12N1/20C12S5/00
CPCY02A50/20Y02P20/59
Inventor 郑连英邓献存
Owner ZHEJIANG UNIV
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