The invention relates to medicine composition for treating burn wound and a preparation method thereof. The medicine composition consists of angelica dahurica, radix paeoniae rubra, radix scrophulariae, rhubarb, radix rehmanniae, angelica, cortex phellodendri, salivia chinensis, rhizoma coptidis, szechuan lovage rhizome, radix paeoniae alba and vegetable oil. The preparation method comprises the steps: placing the angelica dahurica, the radix paeoniae rubra, the radix scrophulariae, the rhubarb, the radix rehmanniae, the angelica, the cortex phellodendri, the salivia chinensis, the rhizoma coptidis, the szechuan lovage rhizome, the radix paeoniae alba and the vegetable oil into a gauze after oil-immersion, mixing, heating and filtering. The medicine composition belongs to the secret recipe of the pure Chinese Traditional medicine, uses traditional medicine materials as raw materials and is prepared by adopting scientific proportion so as to dredge the channels and collaterals, remove pattogenic heat from the blood and toxic material from the body, activate blood and dissolve stasis, relieve swelling and pain and remove the rotten and let fresh grow, recover energy with biochemical operation, activate metabolism and enhance self-immunity function so that the surface of the wound hurt by the burn wound can obtain regenerative repair. The medicine composition can be used for treating various kinds of fire burn, scalding, trauma, abscess, bedsore, scarring and angiitis, various kinds of skin infections, and the like. Due to no pain for treating the burn injury without skin graft, smooth wound surface treated in third-degree burn injury, no obvious scar, the medicine composition is an external treatment with no toxity, no incitement, no senitization, safe and good healing efficacy, and can be applied to emergency treatment for burn injury.