Preparation method of composite microecologics for fur-bearing animals
A technology of compound micro-ecology and fur animals, applied in animal feed, animal feed, food science and other directions to achieve the effect of improving immunity
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Embodiment 1
[0024] The preparation method of compound probiotics comprises the steps:
[0025] (1) Activation and preservation of each slant strain, shake flask culture respectively, LB medium for yeast, lactic acid bacteria, white rot fungus, Bacillus subtilis, bifidobacterium, mold and cellulase, pH7-7.5, 100-130 ℃ autoclave for 20 minutes, incubate at 38 ℃ for 12 hours;
[0026] (2) Primary liquid expansion culture (50L tank) of yeast, lactic acid bacteria, white rot fungus, Bacillus subtilis, bifidobacteria, mold and cellulase, the inoculum size is 10%-20%;
[0027] (3) Secondary liquid expansion culture (500L tank) of yeast, lactic acid bacteria, white rot fungus, Bacillus subtilis, bifidobacteria, mold and cellulase, the inoculum size is 10%-20%;
[0028] (4) Mix yeast, lactic acid bacteria, white rot fungus, Bacillus subtilis, bifidobacterium, mold and cellulase fermentation broth according to the following ratio: 20 parts of yeast, 20 parts of lactic acid bacteria, 20 parts of wh...
Embodiment 2
[0030] The preparation method of compound probiotics comprises the steps:
[0031] (1) Activation and preservation of each slant strain, shake flask culture respectively, LB medium for yeast, lactic acid bacteria, white rot fungus, Bacillus subtilis, bifidobacterium, mold and cellulase, pH7-7.5, 100-130 ℃ autoclave for 40 minutes, incubate at 30 ℃ for 15 hours;
[0032] (2) Primary liquid expansion culture (50L tank) of yeast, lactic acid bacteria, white rot fungus, Bacillus subtilis, bifidobacteria, mold and cellulase, the inoculum size is 10%-20%;
[0033] (3) Secondary liquid expansion culture (500L tank) of yeast, lactic acid bacteria, white rot fungus, Bacillus subtilis, bifidobacteria, mold and cellulase, the inoculum size is 10%-20%;
[0034] (4) Mix yeast, lactic acid bacteria, white rot fungus, Bacillus subtilis, bifidobacterium, mold and cellulase fermentation liquid according to the following ratio: 30 parts of yeast, 30 parts of lactic acid bacteria, 30 parts of w...
Embodiment 3
[0036] The preparation method of compound probiotics comprises the following steps:
[0037] (1) Activation and preservation of each slant strain, shake flask culture respectively, LB medium for yeast, lactic acid bacteria, white rot fungus, Bacillus subtilis, bifidobacterium, mold and cellulase, pH7-7.5, 100-130 ℃ autoclave for 30 minutes, incubate at 35 ℃ for 16 hours;
[0038] (2) Primary liquid expansion culture (50L tank) of yeast, lactic acid bacteria, white rot fungus, Bacillus subtilis, bifidobacteria, mold and cellulase, the inoculum size is 10%-20%;
[0039] (3) Secondary liquid expansion culture (500L tank) of yeast, lactic acid bacteria, white rot fungus, Bacillus subtilis, bifidobacteria, mold and cellulase, the inoculum size is 10%-20%;
[0040] (4) Mix yeast, lactic acid bacteria, white rot fungus, Bacillus subtilis, bifidobacterium, mold and cellulase fermentation broth according to the following ratio: 40 parts of yeast, 40 parts of lactic acid bacteria, 40 p...
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