Preparation method of composite microecologics for fur-bearing animals

A technology of compound micro-ecology and fur animals, applied in animal feed, animal feed, food science and other directions to achieve the effect of improving immunity

Inactive Publication Date: 2017-03-15
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, the research on the compound microecological preparation of fur animals is relatively rare. If a kind of compound microecological preparation can be studied and used in the farm, the long-term use of the compound microecological preparation in the farm will further improve

Method used

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  • Preparation method of composite microecologics for fur-bearing animals
  • Preparation method of composite microecologics for fur-bearing animals
  • Preparation method of composite microecologics for fur-bearing animals

Examples

Experimental program
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Effect test

Embodiment 1

[0024] The preparation method of compound probiotics comprises the steps:

[0025] (1) Activation and preservation of each slant strain, shake flask culture respectively, LB medium for yeast, lactic acid bacteria, white rot fungus, Bacillus subtilis, bifidobacterium, mold and cellulase, pH7-7.5, 100-130 ℃ autoclave for 20 minutes, incubate at 38 ℃ for 12 hours;

[0026] (2) Primary liquid expansion culture (50L tank) of yeast, lactic acid bacteria, white rot fungus, Bacillus subtilis, bifidobacteria, mold and cellulase, the inoculum size is 10%-20%;

[0027] (3) Secondary liquid expansion culture (500L tank) of yeast, lactic acid bacteria, white rot fungus, Bacillus subtilis, bifidobacteria, mold and cellulase, the inoculum size is 10%-20%;

[0028] (4) Mix yeast, lactic acid bacteria, white rot fungus, Bacillus subtilis, bifidobacterium, mold and cellulase fermentation broth according to the following ratio: 20 parts of yeast, 20 parts of lactic acid bacteria, 20 parts of wh...

Embodiment 2

[0030] The preparation method of compound probiotics comprises the steps:

[0031] (1) Activation and preservation of each slant strain, shake flask culture respectively, LB medium for yeast, lactic acid bacteria, white rot fungus, Bacillus subtilis, bifidobacterium, mold and cellulase, pH7-7.5, 100-130 ℃ autoclave for 40 minutes, incubate at 30 ℃ for 15 hours;

[0032] (2) Primary liquid expansion culture (50L tank) of yeast, lactic acid bacteria, white rot fungus, Bacillus subtilis, bifidobacteria, mold and cellulase, the inoculum size is 10%-20%;

[0033] (3) Secondary liquid expansion culture (500L tank) of yeast, lactic acid bacteria, white rot fungus, Bacillus subtilis, bifidobacteria, mold and cellulase, the inoculum size is 10%-20%;

[0034] (4) Mix yeast, lactic acid bacteria, white rot fungus, Bacillus subtilis, bifidobacterium, mold and cellulase fermentation liquid according to the following ratio: 30 parts of yeast, 30 parts of lactic acid bacteria, 30 parts of w...

Embodiment 3

[0036] The preparation method of compound probiotics comprises the following steps:

[0037] (1) Activation and preservation of each slant strain, shake flask culture respectively, LB medium for yeast, lactic acid bacteria, white rot fungus, Bacillus subtilis, bifidobacterium, mold and cellulase, pH7-7.5, 100-130 ℃ autoclave for 30 minutes, incubate at 35 ℃ for 16 hours;

[0038] (2) Primary liquid expansion culture (50L tank) of yeast, lactic acid bacteria, white rot fungus, Bacillus subtilis, bifidobacteria, mold and cellulase, the inoculum size is 10%-20%;

[0039] (3) Secondary liquid expansion culture (500L tank) of yeast, lactic acid bacteria, white rot fungus, Bacillus subtilis, bifidobacteria, mold and cellulase, the inoculum size is 10%-20%;

[0040] (4) Mix yeast, lactic acid bacteria, white rot fungus, Bacillus subtilis, bifidobacterium, mold and cellulase fermentation broth according to the following ratio: 40 parts of yeast, 40 parts of lactic acid bacteria, 40 p...

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Abstract

The invention discloses a preparation method of composite microecologics for fur-bearing animals, and belongs to the technical field of the microecologics for the fur-bearing animals. The preparation method includes performing primary liquid amplification culture on saccharomycetes, lactic acid bacteria, white rot fungi, bacillus subtilis, bifidobacterium, mould and cellulase which are obtained from culture; performing secondary liquid amplification culture on the saccharomycetes, the lactic acid bacteria, the white rot fungi, the bacillus subtilis, the bifidobacterium, the mould and the cellulase which are subjected to the primary liquid amplification culture; mixing saccharomycetes liquor, lactic acid bacteria liquor, white rot fungi liquor, bacillus subtilis liquor, bifidobacterium liquor, mould liquor and cellulase liquor which are obtained after the secondary liquid amplification culture according to a certain weight ratio to obtain the composite microecologics. The composite microecologics obtained according to the preparation method can improve body immunity of the fur-bearing animals while treating bacterial and viral diseases.

Description

technical field [0001] The invention relates to the technical field of fur animal microecological preparations, in particular to a preparation method of fur animal composite microecological preparations. Background technique [0002] Farms (households) add antibiotics to the feed for a long time in the process of breeding fur animals, and do not pay attention to the control of the breeding environment, resulting in serious pollution, which cannot completely resist the infection of germs and viruses for fur animals, and keeps fur animals in a long-term environment. Sub-health state. A large number of experimental studies have shown that the compound microecological preparation can dynamically regulate the ecosystem of animal gastrointestinal flora. It has the ability to promote animal growth and development, improve feed conversion rate, improve the quality of livestock and poultry products, enhance animal immunity, and improve the ability to resist bacteria and viruses. ...

Claims

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Application Information

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IPC IPC(8): A23K10/18A23K20/189A23L3/015
CPCA23L3/0155
Inventor 温建新庄桂玉于艳霞刘慧
Owner QINGDAO AGRI UNIV
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