Preparation method of cell-loaded biological microcapsules

A technology of cell biology and microcapsules, which is applied in the fields of fixed on/in organic carriers, medical science, prostheses, etc., and can solve problems such as difficult hollowing, different sizes, and high leakage

Inactive Publication Date: 2012-10-03
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The materials used in the present invention are cheap and easy to obtain, and the new technology involved has a simple route and high technical content, and the product contains a large amount of cells, has good isolation and self-degradation performance, and is non-toxic to cells and organisms, and is expected to replace the currently used The traditional materials and preparation methods solve the problems of different sizes, difficulty in hollowing, improper size, high leakage and high risk in the use of similar materials in the preparation process of capsules

Method used

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  • Preparation method of cell-loaded biological microcapsules
  • Preparation method of cell-loaded biological microcapsules
  • Preparation method of cell-loaded biological microcapsules

Examples

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Embodiment 1

[0020] 1. Degradation treatment of cationic polyelectrolyte polysaccharides such as gelatin, polyglucosamine, hydroxymethyl chitosan, chitosan and chitooligosaccharide, taking hydroxymethyl chitosan, chitooligosaccharide and gelatin as examples:

[0021] Preparation of low molecular weight hydroxymethyl chitosan with hydroxymethyl chitosan:

[0022] The reaction is carried out in a heterogeneous system. Hydroxymethyl chitosan is first dispersed in deionized water, then hydrogen peroxide is added at a mass ratio of 1:1, and the degradation reaction is carried out in a constant temperature water bath. The reaction time is 8-10 hours to control The molecular weight of the product, the steps of the specific degradation reaction are as follows: After installing the experimental equipment according to the experimental requirements, add the weighed (3.000g) hydroxymethyl chitosan, and then add deionized water at a mass ratio of 25:1 (W:W) 75g, add 78g of hydrogen peroxide, fully disp...

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Abstract

The invention relates to a preparation method of cell-loaded biological microcapsules, which comprises the following preparation steps: a, mixing an anion surfactant with an oil phase to prepare a water-in-oil inverse emulsion system, and mixing the oil phase in the anion surfactant to obtain the water-in-oil inverse emulsion system, so that the mixed solution is oyster white, and is not stratified after standing at room temperature for 30min; b, degrading cation polyelectrolyte polysaccharide to obtain cation polyelectrolyte polysaccharide whose molecular weight is 2/3 that of the cation polyelectrolyte polysaccharide before degradation; c, adding cation polyelectrolyte polysaccharide with low molecular weight degraded in the step b into the water-in-oil inverse emulsion system prepared in the step a, and polymerizing to form capsules by a self-assembling reaction; and d, performing solid-liquid separation for the capsule-containing emulsion system obtained in the step c to obtain solid microcapsules.

Description

technical field [0001] The invention belongs to the technical field of preparation of auxiliary materials and carriers, and in particular relates to a method for preparing cell-loaded biological microcapsules. Background technique [0002] For tissue engineering, it has been found that implanting stem cells or related cells on the surface of necrotic bone through the carrier can effectively treat osteonecrosis; for bioengineering, the cell carrier can avoid the interference of related proteins in the body, reach the lesion correctly, and realize Topical administration; for clinical applications, granule-carrying cells are easier to perform surgery and ensure the activity of cells during treatment. [0003] Making microcapsules of natural products without immunosuppression can solve many problems faced by tissue engineering, bioengineering and clinical applications, and has certain positive significance for the field of biomedicine. As a natural product without immunosuppres...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12N11/04A61L27/50A61L27/54
Inventor 王婷冯章启何农跃
Owner SOUTHEAST UNIV
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