Fermentation method for improving yield of isoleucine

A technology of isoleucine and a fermentation method, which is applied in the field of fermentation to improve the yield of isoleucine, can solve the problems such as the cost of branched-chain amino acids cannot be reduced, the quality cannot reach the pharmaceutical grade, the price cannot rise, and the like. Conducive to respiratory metabolism, beneficial to purity, and to avoid corrosion

Inactive Publication Date: 2015-03-25
HENAN JULONG BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the cost of branched-chain amino acids in my country cannot be reduced, and the quality of the products is poor, and the price cannot go up. Most of the branched-chain amino acids can only be used in the production of feed, and the quality cannot reach the pharmaceutical grade.
In addition, the highest domestic acid production rate reaches 28-33 g / L, while isoleucine has achieved large-scale industrial production abroad, and the acid production level has reached above 30-35 g / L; in terms of production rate, the current fermentation The highest conversion rate of isoleucine produced by the method is only 18-20%

Method used

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  • Fermentation method for improving yield of isoleucine
  • Fermentation method for improving yield of isoleucine
  • Fermentation method for improving yield of isoleucine

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Embodiment 1

[0047] A fermentation method for increasing the yield of isoleucine, the steps are as follows:

[0048] Step 1. Cultivate the bacteria

[0049] Streak inoculate the bacteria on the activated slant, culture at a constant temperature of 31°C for 36-40 hours, expand the culture in the strain room to produce first-level seeds, and then transfer the obtained first-level seeds to the second-level seed tank to continue the expansion and cultivation, and then expand the cultivation to the second-level Sterile air is passed into the seed culture medium, and the ventilation rate is 0.5V / V / M, and the secondary seeds are eliminated, cooled, and cultivated in the secondary seed tank to obtain the secondary seed culture solution, which is set aside; Grade seed medium is composed of raw materials with the following mass concentrations: glucose 25g / L, magnesium sulfate 0.5g / L, potassium dihydrogen phosphate 1.3g / L, ammonium sulfate 5g / L, yeast extract powder 2.2g / L, corn steep liquor hydrolys...

Embodiment 2

[0067] A fermentation method for increasing the yield of isoleucine, the steps are as follows:

[0068] Step 1. Cultivate the bacteria

[0069] Streak inoculate the bacteria on the activated slant, culture at a constant temperature of 31°C for 36-40 hours, expand the culture in the strain room to produce first-level seeds, and then transfer the obtained first-level seeds to the second-level seed tank to continue the expansion and cultivation, and then expand the cultivation to the second-level Sterile air is passed into the seed culture medium, and the ventilation rate is 0.8V / V / M, and the secondary seeds are eliminated, cooled, and cultivated in the secondary seed tank to obtain the secondary seed culture solution, which is set aside; Grade seed medium is composed of raw materials with the following mass concentrations: glucose 35g / L, magnesium sulfate 0.3g / L, potassium dihydrogen phosphate 1.3g / L, ammonium sulfate 5g / L, yeast extract powder 2.2g / L, corn steep liquor hydrolys...

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Abstract

The invention discloses a fermentation method for improving the yield of isoleucine. The fermentation method comprises the following steps: culturing microorganisms, fermenting, separating fermentation liquor, carrying out ion exchange on fermentation supernatant, deaminizing with a deamination tower, decolorizing with activated carbon and crystallizing by evaporating. Because the corn steep liquor which serves as a hydrolysis liquor in the culture medium and accounts for 40% of the total weight of the culture medium replaces the hydrochloric acid adopted by the traditional method to regulate the pH value, the corrosion of the hydrochloric acid to the stainless steel tank is avoided. In addition, because biotin and VB1 are used as growth factors in the process, the viscosity of the fermentation liquor can be reduced, the respiratory metabolism of the microorganisms can be promoted, and the down-stream finished product can be extracted easily. By adopting the fermentation method disclosed by the invention, a good fermentation index can be achieved, the yield of the isoleucine which is a metabolite can be improved significantly, the average yield of the isoleucine can be up to 36.9g/L and can be increased by 30% compared with that of the metabolite isoleucine obtained by adopting the original method, the conversion rate of the metabolite isoleucine can be increased by 2-4%, and the fermentation cycle can be shortened by 10 hours.

Description

technical field [0001] The invention belongs to the technical field of amino acid production, and in particular relates to a fermentation method for increasing the yield of isoleucine. Background technique [0002] As one of the branched-chain amino acids, L-isoleucine is an essential amino acid for the human body and has various physiological functions. Because of its special structure and function, it occupies a particularly important position in the metabolism of human life. It is mainly used to prepare amino acid infusions, synthetic polypeptide drugs and food antioxidants, etc., and its role in medical research and treatment is increasingly valued. It is used in the treatment of blood-brain barrier, hepatic coma, chronic liver cirrhosis and renal failure, dietary treatment of congenital metabolic defects, treatment of table blood and postoperative diabetic patients, acceleration of surgical wound healing, nutritional support treatment of tumor patients widely used in....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/06
Inventor 李静刘帅刘晓都
Owner HENAN JULONG BIOLOGICAL ENG CO LTD
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