Method for measuring activity of packing biological membranes of artificial wetlands

A technology of activity determination and biofilm, applied in the direction of chemical method analysis, measuring device, analysis material, etc., can solve the problems that cannot fully and accurately reflect the metabolic capacity of biofilm, cannot reflect the real activity of biofilm, and achieve more convincing results High force, stable measurement results, simple and convenient operation

Inactive Publication Date: 2012-08-15
叶春 +2
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  • Application Information

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Problems solved by technology

However, in the biofilm process, from the innermost layer to the outermost layer of the biofilm, there are anaerobic zone, anoxic zone, and aerobic zone in sequence, and anaerobic microorganisms, facultative microorganisms, and aerobic microorganisms are correspondingly distributed, and The quantity of anaerobic microorganisms and facultative microorganisms in the wetland environment is far greater than that of aerobic microorganisms; therefore, only the consumption rate of oxygen is measured, and the electron acceptors (usually nitrate, sulfate, Iron ions) concentration changes cannot comprehensively and accurately reflect the metabolic capacity of the biofilm, that is, it cannot reflect the real activity of the biofilm

Method used

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  • Method for measuring activity of packing biological membranes of artificial wetlands
  • Method for measuring activity of packing biological membranes of artificial wetlands
  • Method for measuring activity of packing biological membranes of artificial wetlands

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example 1

[0075] In this example, using the method of the present invention, the artificial wetland filler zeolite is used as the research object, and the biofilm activity of the biofilm with a film formation time of 2 days, 8 days, and 14 days is measured, and the change of the film activity with the length of the film formation time is compared. trend. In this embodiment, the devices used include:

[0076] (1) Magnetic stirrer;

[0077] (2) Magnetic bar;

[0078] (3) dissolved oxygen meter (Swiss Mettler company, instrument model HACH HQ30d);

[0079] (4) Fixed power supply (220V, 50Hz);

[0080] (5) Spectrophotometer (Shanghai Puyuan a-1860, 721 UV spectrophotometer)

[0081] (6) 250ml jars.

[0082] Each time (respectively the 2nd day, 8th day, and 14th day after the film was set up) sampling analysis, do 3 parallel samples, including the following steps:

[0083] (1) Put 20g of artificial wetland filler (zeolite) with biofilm on it into a pre-sterilized 250mL jar, and put it ...

Embodiment 2

[0128] In this example, this method is used to compare the biofilm activity of two kinds of constructed wetland fillers, zeolite and ceramsite, in the stable period of film formation, so as to judge which kind of filler is more suitable for application in constructed wetlands. In this embodiment, the devices used include:

[0129] (1) Magnetic stirrer;

[0130] (2) Magnetic bar;

[0131] (3) dissolved oxygen meter (Swiss Mettler company, instrument model HACH HQ30d);

[0132] (4) Fixed power supply (220V, 50Hz);

[0133] (5) Spectrophotometer (Shanghai Puyuan a-1860, 721 UV spectrophotometer)

[0134] (6) 250ml jars.

[0135] According to the pre-test results, it was found that the two artificial wetland fillers reached a stable state 22 days after the film was formed. Therefore, the zeolite and ceramsite with the film were taken on this day, and three parallel samples were made for each to measure the biofilm activity. The specific operation steps are as follows:

[013...

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Abstract

The invention discloses a method for measuring the activity of packing biological membranes of artificial wetlands. The method includes simultaneously monitoring changes of the concentration of common electron acceptors in respiratory metabolism of aerobic microorganisms, anaerobic microorganisms and facultative microorganisms according to characteristics of metabolisms of the aerobic microorganisms, the anaerobic microorganisms and the facultative microorganisms in a packing biological membrane of an artificial wetland so that the degree of the activity of the biological membrane is reflected. In the method, the activity of the biological membrane is reflected by means of measuring the changes of the concentration of dissolved oxygen (DO) content, nitrate (NO3-), sulfate radicals (SO42-) and ferric ions [(Fe(III)], a computation formula is provided, and a theoretical basis is reliable. The method has the advantages that results are accurate and stable, interferences are few, operation is simple and speedy, and the method is technically feasible and economically reasonable.

Description

technical field [0001] The invention relates to the field of artificial wetlands, in particular to the measurement of biofilm activity of artificial wetland fillers. Background technique [0002] The adsorption, degradation and transformation of organic matter in the constructed wetland system are mainly completed by the biofilm, and the biological activity of the biofilm directly affects the treatment efficiency of the constructed wetland system. Therefore, how to determine the level of biofilm activity is very important in order to adjust the operating conditions of the constructed wetland system in time to achieve the best operating state. At present, the methods used to measure biofilm activity mainly include various enzyme activity assays and oxygen consumption rate methods, but these methods have many disadvantages when applied to the biofilm activity assay of constructed wetland fillers: [0003] (1) Determination of biological enzyme activity: the principle of this ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N31/00
Inventor 叶春李春华姜怡
Owner 叶春
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