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Biopesticides for potato late blight disease

A potato late blight, biological technology, applied in the direction of biocides, chemicals for biological control, fungicides, etc.

Active Publication Date: 2019-05-03
AGRI & AGRI FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Phytophthora infestans is extremely adaptable, and various new genotypes have developed resistance to major fungicides, including metalaxyl and mefenoxam, or overcome crop resistance sexual ability

Method used

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  • Biopesticides for potato late blight disease
  • Biopesticides for potato late blight disease
  • Biopesticides for potato late blight disease

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0035] Example 1: Microbial Culture

[0036] Bacterial Suspension:

[0037] Bacterial cultures maintained at -80°C were thawed and plated on Pseudomonas agar F medium (containing Difco TM Casein pancreatic digest (10.0g), No. 3 proteose peptone (10.0g), K 2 HPO 4 (1.5g), MgSO 4 (1.5g), agar (15.0g) and glycerol (10g) in water (1L)). The colony was inoculated into 125mL yeast extract glucose medium (YGM; containing yeast extract (2.0g), dextrose (2.5g), buffer solution (10mL, KH 2 PO 4 (25.0g / L) and K 2 HPO 4 (25.0g / L)) and brine solution (10mL, MgSO 4 ·7H 2 O(10.0g / L), MnSO 4 ·H 2 O(1.5g / L), NaCl(5.0g / L) and FeSO 4 ·7H 2 (0.5 g / L)) in water (1 L total volume)) and incubated for 48 hours at 22° C. in a 500 mL baffled flask on a rotary shaker (150 rpm) to obtain a bacterial suspension.

[0038] Cell-free bacterial filtrate:

[0039] The bacterial suspension was centrifuged at 10,000 rpm for 10 minutes, and the supernatant was vacuum filtered through a 0.22 μm non-...

example 2

[0042] Example 2: In Vitro Bioanalysis

[0043] bacterial suspension

[0044] An agar plunger (0.5 cm diameter) of Phytophthora infestans (A1 or A2 mating type) was placed in the center of a Petri dish (9 cm diameter) containing Rye Seed A Agar (RSA). Aliquots (2 μL) of the bacterial suspension were placed close to the edge of the plate; 2-4 bacterial strains were tested on each plate. After 7 days of incubation at 15°C, the zone of inhibition was measured. Two experiments were performed, each using four replicates.

[0045] cell-free bacterial filtrate

[0046] Cell-free bacterial filtrates or sterile water as a negative control were dispensed on agar (50%, v / v). A mycelial plunger of the pathogen was placed in the center of a Petri dish (9 cm diameter) and after incubation at 22°C for 7 and 12 days, mycelial growth was measured. Two experiments were performed, each using four replicates. use SAS TM software to analyze the results. The control percentage (%) is calcul...

example 3

[0051] Example 3: Bioanalysis of in vivo feeding detached leaves

[0052] Potato leaves each containing 5 leaflets were selected and grown in 50 mL tubes containing 10% Hoagland's solution (Hoagland, D.R. and Arnon, D.I. "with The water-culture method for growing plants without soil" Univ.Calif.Coll.Agric.Exp.Sta.Circ, University of California, Berkeley, CA. . Berkeley, CA) (1938), 347-353). The leaves were dipped in the selected test or control treatment as described below and after 2 hours were sprayed with the Phytophthora infestans suspension until run off. The treated leaves were incubated at 22°C under high humidity conditions (86%-92% relative humidity) and a photoperiod of 16 hours per day / 8 hours per night.

[0053]After 7 and 10 days of incubation, disease progression and severity on leaves were measured by estimating the proportion of the photosynthetic area affected by the pathogen (James, 1971: James, W.C. Keys to plant disease assessment, their preparation and ...

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Abstract

Bacterial strains, or extracts thereof, are effective to control, treat or prevent infection of solanaceous plants with Phytophthora infestans, the causative agent of potato late blight disease. Alsoprovided are biopesticidal formulations containing one or more of the bacterial strains or extracts thereof, and the use of the bacterial strains, extracts or biopesticidal formulations in the control, treatment and / or prevention of potato late blight disease.

Description

Background technique [0001] This application relates to biopesticides for the treatment, control and / or prevention of potato late blight. More specifically, the present application relates to bacterial strains suitable for controlling the pathogen causing late blight of potatoes. [0002] Potato late blight, notorious for its involvement in the Irish potato famine of the 1840s, was caused by pathogenic oomycetes (Saprolegnia spp.) in potato plants (Solanum tuberosum L.) and other nightshade crops such as tomato and eggplant Phytophthora (Phytophthora infestans (Mont.) de Bary) infection. Infection is characterized by black / brown lesions on plant stems and leaves that expand rapidly and become necrotic. Harvested diseased potato tubers may rot while in storage, or if they survive the winter in the storage facility or in the soil, they may spread the disease into next year's crop. [0003] Current control measures involve an integrated approach to pest control, including popu...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01N63/00A01P3/00A01N63/20A01N63/22A01N63/27
CPCA01N63/27A01N63/22A01N63/20C12N1/20
Inventor S·鲍伊特基柯P·奥迪K·萨瓦奇耶恩R·迪斯贾格尼斯周婷T·杜蒙斯奥克斯
Owner AGRI & AGRI FOOD
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