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Test method of developmental toxicity of silkworms by environmental estrogen

Active Publication Date: 2019-04-02
SUZHOU UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method of adding 4-NP to the whole age is time-consuming and has certain difficulties in the breeding operation of silkworms.

Method used

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  • Test method of developmental toxicity of silkworms by environmental estrogen
  • Test method of developmental toxicity of silkworms by environmental estrogen

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Experimental program
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Embodiment Construction

[0022] The present invention will be described in detail below with reference to the accompanying drawings and in combination with embodiments.

[0023] A method for testing the developmental toxicity of environmental estrogens to silkworms, comprising the following steps:

[0024] Step 1) First, weigh 1176 mg of 4-NP and dissolve it in 50 ml of absolute ethanol to prepare a 20 g / L 4-NP solution. After filtering this solution with a 0.22 μm microporous membrane, use it as 4- Transfer the NP mother solution into the reagent bottle and seal it with a film, then store it in a refrigerator at 4°C and avoid light;

[0025] Step 2) During the experiment, take out the above mother liquor, after returning to room temperature and shaking well, prepare 100ml of 4-NP exposure solutions with concentrations of 0.02, 0.20, and 2.0 g / L with ultrapure water and absolute ethanol, so that each The concentration of anhydrous ethanol in the group was 10%, and the reagent bottle was sealed with a...

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Abstract

The invention discloses a test method of developmental toxicity of silkworms by environmental estrogen. The method includes following steps: firstly, injecting a test sample containing a series of concentrations to 5-age silkworm larvae through 2-hour starvation treatment by employing oral gavage; after 20 minutes, performing anatomy on the silkworms through gavage, and taking tissues and organs;determining the developmental toxicity of the silkworms by the test sample through comparison of toxic endpoints of an infected group and a control group, wherein the toxic endpoints comprise a glutathione reductase activity coefficient and an anti-superoxide anion free radical activity of midguts of the silkworms; and researching the influence of the environmental estrogen 4-nonylphenol on the development of the midguts of the silkworms in a larva stage to determine a dose-effect relation between the environmental estrogen and the developmental toxicity of the silkworms in the larva stage. Byemploying the technical scheme, multiple biological effect endpoints can be generated for the environmental estrogen nonylphenol, potential risks of the silkworms exposed in an estrogen environmentalconcentration level are evaluated, and the method is a relatively fast, simple and sensitive detection method.

Description

technical field [0001] The invention relates to the field of biology, in particular to a method for testing the developmental toxicity of environmental estrogens to silkworms. Background technique [0002] Environmental hormones are synthetic chemical substances that widely exist in nature and exhibit estrogenic effects on invertebrates and vertebrates. Nonylphenol (Nonylphenol, NP) is a substance that has been proven to have estrogenic effects. NP is widely used in industrial and agricultural production processes, and it is stable at room temperature and not easy to be degraded. In recent years, NP has been detected in many rivers and lakes such as Taihu Lake and Songhua River Basin in my country. NP is also detected in organisms such as tap water, milk, animals and plants. NP can enter the organism through various ways and has bioaccumulation. The permanent nature and lipophilicity of NP lead to its tendency to accumulate in animal tissues, and its acute and chronic to...

Claims

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Application Information

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IPC IPC(8): G01N33/74
CPCG01N33/74
Inventor 袁红霞秦粉菊
Owner SUZHOU UNIV OF SCI & TECH
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