16 results about "Polyhedral virus" patented technology

The invention discloses a production process for culturing nuclear polyhedrosis virus by using an S2 or SF9 cell line. The preparation method comprises the following steps: connecting a cell-penetrating peptide C105Y with DSPE-PEG2000-Mal on the surface of a lipid nanoparticle, then preparing a lipid nanoparticle LNP-C105Y by using the DSPE-PEG2000-Mal, and entrapping nuclear polyhedrosis virus DNA to obtain DNAHcNPV LNP-C105Y; the DNAHcNPV coated LNP-C105Y is inoculated into an SF9 grassland spodoptera frugiperda cell line or an S2 fruit fly cell line to be cultured, and the nuclear polyhedrosis virus is produced. On the basis of the fall webworm nuclear polyhedrosis virus, DNAHcNPV-LNP-C105Y lipid nanoparticles are synthesized and prepared by adopting a film dispersion-extrusion method to wrap the fall webworm nuclear polyhedrosis virus, and the fall webworm nuclear polyhedrosis virus is produced by inoculating the fall webworm nuclear polyhedrosis virus to SF9 and S2 cell lines. The hyphantria cunea nuclear polyhedrosis virus production process is improved, and the defect that the hyphantria cunea nuclear polyhedrosis virus production process in the prior art cannot be separated from hyphantria cunea living body feeding is overcome.

The application discloses a production method of a high-efficiency strain of Bupalus piniarius nuclear polyhedrosis virus, and belongs to the technical field of biological pesticide production. 8 The virus yield of the high-efficiency strain of Bupalus piniarius nuclear polyhedrosis virus is 6*10 The virus loss rate is reduced by 1.4% by adding 0.1% detergent before centrifugal purification of the virus, and the high yield of the virus is further ensured.The insecticidal effect of the high-efficiency strain produced by the method is increased by more than 64.1% than that of an ordinary strain, and the high-efficiency property is continuously maintained.The method has a very beneficial effect on the production of the high-efficiency strain of Bupalus piniarius nuclear polyhedrosis virus, and provides a solid guarantee for popularization and application of the high-efficiency strain.

The present invention relates to synthetic designs or chimeric proteins for pseudotyping baculovirus (Autographa californica nuclear polyhedrosis virus) with the rabies virus glycoprotein G (gG) on its surface (Bac::gG-FL), which can be used in anti-rabies vaccine formulations. The chimeric protein is designed from a gene cassette containing gene fragments of the ectodomain of the G glycoprotein of the Pasteur strain rabies virus, a linker of 7 amino acids (GGGGSGG), as well as transmembrane (TM) and cytoplasmic (CT) regions of the gp64 baculovirus protein, with the arrangement of the sequences in the designed gene cassette being shown in figure 1.

The application discloses application of a microbial natural product GameXPeptide-A in improving the control effect of insecticides on pests, and belongs to the technical field of biological control. Experiments prove that GameXPeptide-A can effectively improve the insecticidal activity of insecticides on agricultural pests such as thrips, corn borer and prodenia litura when GameXPeptide-A is combined with insecticides such as Metarrhizium anisopliae, Beauveria bassiana, nuclear polyhedrosis virus, 4% abamectin and pyridaben, 10% dimethoate and spinosad, 30% ethoxazole, 45% bifenthrin and etoxazole, 5% spinosad, 5% chlorantraniliprole, 5% abamectin and the like, and it is indicated that the substance can be combined with different insecticides to improve the insecticidal activity of insecticides on pests such as Lepidoptera, Diptera, Coleoptera, Orthoptera, Hemiptera and Homoptera in agricultural production, and can be used for the development and application of a new type of pest immunosuppressant or insecticide synergist.

The invention discloses a permeation persistent-effect auxiliary agent with a spodoptera exigua nuclear polyhedrosis virus control effect, a preparation and application, and relates to the technical field of agricultural biopesticides. The auxiliary agent comprises a reverse penetrating agent, a leaf back deposition agent, a midgut synergistic agent, an environment activation type adhesive, a system compatible component and the balance of a carrier. Through cooperation of the lecithin / alcohol ether compound reverse penetrating agent and the hydrophobic modified silicon dioxide, liquid medicine canopy penetration and leaf back efficient deposition are achieved; a carrageenan-chitosan compound forms an intelligent response film of'drying viscosity loss-wet activation ', and the lasting period is prolonged to 10-14 days; chitosan oligosaccharide can regulate the midgut environment of pests, and the virus infection rate is increased by 1.8-2.3 times. And the auxiliary agent is compatible with an unmanned aerial vehicle low-volume spraying system and is suitable for green prevention and control of beet armyworms of cruciferous crops such as cabbages, cauliflowers and Chinese cabbages, the control effect reaches 95% or above 7 days after pesticide application, and the lasting period is prolonged by 5-7 days compared with that of a conventional preparation.

The application discloses a production process of culturing nucleotype polyhedrosis virus by using S2 or SF9 cell lines. Cell penetrating peptide C105Y is connected with DSPE-PEG2000-Mal on the surface of a lipid nanoparticle, and then the lipid nanoparticle LNP-C105Y is prepared by using the same, nucleotype polyhedrosis virus DNA is loaded in the lipid nanoparticle, and DNA HcNPV @LNP-C105Y; the DNA HcNPV @LNP-C105Y is inoculated into SF9 Spodoptera frugiperda cell lines or S2 Drosophila cell lines for culture, and nucleotype polyhedrosis virus is produced. The nucleotype polyhedrosis virus of Hyphantria cunea is taken as the basis, a DNA HcNPV @LNP-C105Y lipid nanoparticle is used for wrapping the nucleotype polyhedrosis virus of Hyphantria cunea, and is inoculated into SF9 and S2 cell lines to produce the nucleotype polyhedrosis virus of Hyphantria cunea. The nucleotype polyhedrosis virus production process of the application is improved, and the defect that the existing virus production process cannot be separated from the live feeding of Hyphantria cunea is overcome.

The application discloses a cotton bollworm nuclear polyhedrosis virus spike period targeted synergistic adjuvant, a pesticide preparation and application, relates to the technical field of agricultural biological pesticides, and the composite adjuvant is prepared from ultraviolet protection and shielding materials, film-forming materials, midgut infection promoters, penetration and spreading agents, system compatible components and carriers.The adjuvant is applied by unmanned aerial vehicle top spraying, and after being compounded with HaNPV, the deposition rate of the virus on the corn tassel can be increased by 3.2 times, the retention amount is increased by 5.62 times, the destruction rate of the midgut peritrophic membrane of the cotton bollworm larvae is more than 90%, and the mortality of the 3rd instar cotton bollworm larvae is increased to 87.72%.The application solves the two technical bottlenecks of the virus pesticide, i.e., 'difficult coverage of the tassel target' and 'low midgut infection efficiency', and provides key technical support for green and precise prevention and control of the cotton bollworm in the corn tassel period.

The invention discloses an insecticidal composition containing entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and relates to the technical field of biological insecticide, the active ingredients of the insecticidal composition are entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and the entomopathogenic virus is nuclear polyhedrosis virus of baculoviridae. The weight ratio of the virus to the jasmonic acid methyl ester or jasmonic acid is 1: (0.5-1000), and the insecticidal composition can be prepared into dosage forms such as a suspending agent, water dispersible granules, wettable powder and the like. The invention also provides a method for preventing and treating lepidoptera pests, which comprises the step of applying the entomopathogenic virus and methyl jasmonate or jasmonic acid to target pests or a living environment of the target pests in a combined manner, and the combined application is simultaneous application or successive interval application. The insecticidal composition has a synergistic effect on lepidoptera pests such as cotton bollworms and beet armyworms, can obviously reduce the food intake, accelerate the death of pests and improve the field control effect, and has a wide application prospect.

The present application belongs to the field of bio-technology of agriculture and forestry, and relates to a method for increasing the egg production of Spodoptera litura based on irradiation mode. 60 Co source irradiation treatment; the irradiated Spodoptera litura pupae are cultured in an incubator to emerge, then the adults are moved to an adult rearing cage for rearing, and honey water is provided for nutrition. Through effective accumulated temperature treatment, a large number of Spodoptera litura pupae with consistent development are selected as irradiation objects, which can greatly increase the egg production of Spodoptera litura under the premise of ensuring the emergence rate of Spodoptera litura pupae and the lifespan of adults, increase the production of test insects, and the operation process is simple, safe and reliable, which provides a large amount of production insect source for conveniently and quickly producing biological control agent nuclear polyhedrosis virus by using Spodoptera litura, and expanding the host parasitic wasp by using the insect as host.

The invention provides a gene with an antiviral effect and application thereof, the nucleotide sequence of an open reading frame of the gene is SEQ ID NO: 2, and the amino acid sequence of a coding protein of the gene is SEQ ID NO: 1. The invention also provides a method for increasing the sensitivity of the beet armyworm to the beet armyworm nuclear polyhedrosis virus, which is used for reducing the expression quantity of the gene in the beet armyworm. According to the invention, a gene with an antiviral effect is screened from an important agricultural pest beet armyworm. The sequence structure and tissue expression mode of the gene are analyzed, incremental expression and RNA interference technologies are utilized to prove that the gene has an anti-SeMNPV effect, a new target is provided for better utilizing baculovirus to prevent and control beet armyworms in production, and the gene has a good application prospect.

The invention discloses a yellow thorn moth blood lymphocyte line, an expanded production method of a nuclear polyhedrosis virus and application of the yellow thorn moth blood lymphocyte line and the nuclear polyhedrosis virus. The name of the dalbergia mandshurica blood lymphocyte line is dalbergia mandshurica blood lymphocyte CFW-CHY, the dalbergia mandshurica blood lymphocyte line is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: C2025104, and the preservation date is March 27, 2025; the yellow thorn moth blood lymphocyte line is used for expanded production of the nuclear polyhedrosis virus, so that industrial production of the nuclear polyhedrosis virus is realized, and the yellow thorn moth blood lymphocyte line can be used in the fields of preparation of insecticides, insecticidal pesticides and the like.

The present invention relates to synthetic designs or chimeric proteins for pseudotyping baculovirus (Autographa californica nuclear polyhedrosis virus) with the rabies virus glycoprotein G (gG) on its surface (Bac::gG-FL), which can be used in rabies vaccine formulations. The chimeric protein is designed from a gene cassette containing genetic fragments from the ectodomain of the Pasteur variant rabies virus glycoprotein G, a 7-amino-acid connector sequence (GGGGSGG), as well as transmembrane (TM) and cytoplasmic (CT) regions of the baculoviral protein gp64, the arrangement of which in the designed gene cassette is shown in Figure 1.

The application belongs to the field of biological pesticides, and provides application of HearNPV-miR-56 in cotton bollworm control, wherein the nucleotide sequence of the HearNPV-miR-56 is CGGUAACAGAAGCGGUCACG. The HearNPV-miR-56 can improve virus replication amount of cotton bollworm nuclear polyhedrosis virus infected larvae, thereby promoting death, and can be used for developing a cotton bollworm nuclear polyhedrosis virus synergist, and has potential application prospect in biological control of cotton bollworm.

The application discloses application of apolipoprotein III in prevention and treatment of Bombyx mori nuclear polyhedrosis virus infection and belongs to the cross field of molecular biology technology field and virus prevention and treatment. The application first finds that Bombyx mori apolipoprotein III (ApoLp-III) promotes biosynthesis and accumulation of ceramide, thereby inhibits mTORC1 signal pathway activity, induces cell G1 phase arrest, and effectively blocks a new mechanism of Bombyx mori nuclear polyhedrosis virus (BmNPV) replication. Based on the mechanism, the application provides application of ApoLp-III in inhibition of BmNPV proliferation, prevention and treatment of BmNPV infection, improvement of the ability of Bombyx mori to resist BmNPV or cultivation of a BmNPV-resistant Bombyx mori strain. The application provides a brand-new technical strategy and germplasm resource for realizing green, efficient and sustainable prevention and control of BmNPV.

The invention discloses a method for preventing and controlling prodenia litura nuclear polyhedrosis virus diseases, and belongs to the technical field of large-scale breeding of insects. The method aims at solving the problems that in the prior art, the chemical disinfection effect is limited, and the disease resistance of prodenia litura is difficult to improve. According to the technical scheme, the method mainly comprises the following steps: firstly, disinfecting prodenia litura eggs to reduce the initial virus load; secondly, an immunopotentiator is added into the basal feed, and the immunopotentiator is selected from sodium houttuyfonate, houttuynia cordata powder or a boiling soak solution prepared from houttuynia cordata roots, stems and leaves, so that the immunologic function of the prodenia litura is enhanced, and the resistance of the prodenia litura to nuclear polyhedrosis virus (NPV) is improved. Through verification, the survival rate of prodenia litura infected with NPV in large-scale feeding can be remarkably increased, and the method is easy and convenient to operate, low in cost and environmentally friendly.

Disclosed is a silk gland recombinant expression vector of silkworm expressing human epidermal growth factor, a preparation method and use thereof. The recombinant expression vector is formed by fusing truncated silk fibroin light chain N-terminal domain, C-terminal domain, optimized human epidermal growth factor and silkworm nuclear polyhedrosis virus enhancer, and removes the redundant part of the extra domain of endogenous silk protein contained in the fusion protein to the maximum extent.