33 results about "Polyhedral virus" patented technology

The invention discloses a production process for culturing nuclear polyhedrosis virus by using an S2 or SF9 cell line. The preparation method comprises the following steps: connecting a cell-penetrating peptide C105Y with DSPE-PEG2000-Mal on the surface of a lipid nanoparticle, then preparing a lipid nanoparticle LNP-C105Y by using the DSPE-PEG2000-Mal, and entrapping nuclear polyhedrosis virus DNA to obtain DNAHcNPV LNP-C105Y; the DNAHcNPV coated LNP-C105Y is inoculated into an SF9 grassland spodoptera frugiperda cell line or an S2 fruit fly cell line to be cultured, and the nuclear polyhedrosis virus is produced. On the basis of the fall webworm nuclear polyhedrosis virus, DNAHcNPV-LNP-C105Y lipid nanoparticles are synthesized and prepared by adopting a film dispersion-extrusion method to wrap the fall webworm nuclear polyhedrosis virus, and the fall webworm nuclear polyhedrosis virus is produced by inoculating the fall webworm nuclear polyhedrosis virus to SF9 and S2 cell lines. The hyphantria cunea nuclear polyhedrosis virus production process is improved, and the defect that the hyphantria cunea nuclear polyhedrosis virus production process in the prior art cannot be separated from hyphantria cunea living body feeding is overcome.

The invention belongs to the technical field of biological control, and relates to a preparation method of prodenia litura nuclear polyhedrosis virus concentrate, which comprises the following steps: cultivating and hatching prodenia litura eggs at constant temperature, culturing the prodenia litura eggs in a dark place into 4-day-old larvae, performing virus infection and proliferation on the 4-day-old larvae, collecting and screening dead insects with strong toxicity and early death as virus semi-finished products, and refrigerating and storing the virus semi-finished products. And finally, preparing the virus semi-finished product into mother powder to obtain the prodenia litura nuclear polyhedrosis virus mother drug. The method comprises the following steps: extracting a parent drug sample to obtain a virus polyhedrosis, extracting a DNA comparison gene sequence of the virus polyhedrosis, prodenia litura nuclear polyhedrosis virus which is identified as prodenia litura nuclear polyhedrosis virus when the similarity between the virus polyhedrosis and Spodoptera litura Nucleoplydrovirus reaches 80% or above, and measuring various performance indexes, so that the prodenia litura nuclear polyhedrosis virus can be identified as prodenia litura nuclear polyhedrosis virus. The prodenia litura nuclear polyhedrosis virus mother drug prepared by the preparation method is used for preventing and treating lepidoptera pests such as prodenia litura, has important significance, has good development and application prospects, and has application value in preparation of insecticides and prevention and treatment of flat thorn moths.

The application discloses a production method of a high-efficiency strain of Bupalus piniarius nuclear polyhedrosis virus, and belongs to the technical field of biological pesticide production. 8 The virus yield of the high-efficiency strain of Bupalus piniarius nuclear polyhedrosis virus is 6*10 The virus loss rate is reduced by 1.4% by adding 0.1% detergent before centrifugal purification of the virus, and the high yield of the virus is further ensured.The insecticidal effect of the high-efficiency strain produced by the method is increased by more than 64.1% than that of an ordinary strain, and the high-efficiency property is continuously maintained.The method has a very beneficial effect on the production of the high-efficiency strain of Bupalus piniarius nuclear polyhedrosis virus, and provides a solid guarantee for popularization and application of the high-efficiency strain.

The invention belongs to the technical field of agricultural biology, and particularly relates to application of an endoplasmic reticulum protein antioxidant (reducing agent) in prevention and treatment of bombyx mori nuclear polyhedrosis virus. A screening test finds that the related endoplasmic reticulum protein antioxidants beta-mercaptoethanol (BME), dithiothreitol (DTT) and / or tris (2-carboxyethyl) phosphine (TCEP) have the effect of inhibiting the bombyx mori nuclear polyhedrosis virus (BmNPV), and the antioxidants interfere the replication and proliferation of the virus by destroying disulfide bonds in key proteins of the BmNPV, so that the bombyx mori nuclear polyhedrosis virus can be inhibited. The stability and activity of virions are directly influenced, so that the infection of the viruses on the silkworms is inhibited, a brand new thought is provided for the development of antiviral drugs of the silkworms, and the application value is good.

The present invention relates to synthetic designs or chimeric proteins for pseudotyping baculovirus (Autographa californica nuclear polyhedrosis virus) with the rabies virus glycoprotein G (gG) on its surface (Bac::gG-FL), which can be used in anti-rabies vaccine formulations. The chimeric protein is designed from a gene cassette containing gene fragments of the ectodomain of the G glycoprotein of the Pasteur strain rabies virus, a linker of 7 amino acids (GGGGSGG), as well as transmembrane (TM) and cytoplasmic (CT) regions of the gp64 baculovirus protein, with the arrangement of the sequences in the designed gene cassette being shown in figure 1.

The application discloses application of a microbial natural product GameXPeptide-A in improving the control effect of insecticides on pests, and belongs to the technical field of biological control. Experiments prove that GameXPeptide-A can effectively improve the insecticidal activity of insecticides on agricultural pests such as thrips, corn borer and prodenia litura when GameXPeptide-A is combined with insecticides such as Metarrhizium anisopliae, Beauveria bassiana, nuclear polyhedrosis virus, 4% abamectin and pyridaben, 10% dimethoate and spinosad, 30% ethoxazole, 45% bifenthrin and etoxazole, 5% spinosad, 5% chlorantraniliprole, 5% abamectin and the like, and it is indicated that the substance can be combined with different insecticides to improve the insecticidal activity of insecticides on pests such as Lepidoptera, Diptera, Coleoptera, Orthoptera, Hemiptera and Homoptera in agricultural production, and can be used for the development and application of a new type of pest immunosuppressant or insecticide synergist.

The invention discloses a permeation persistent-effect auxiliary agent with a spodoptera exigua nuclear polyhedrosis virus control effect, a preparation and application, and relates to the technical field of agricultural biopesticides. The auxiliary agent comprises a reverse penetrating agent, a leaf back deposition agent, a midgut synergistic agent, an environment activation type adhesive, a system compatible component and the balance of a carrier. Through cooperation of the lecithin / alcohol ether compound reverse penetrating agent and the hydrophobic modified silicon dioxide, liquid medicine canopy penetration and leaf back efficient deposition are achieved; a carrageenan-chitosan compound forms an intelligent response film of'drying viscosity loss-wet activation ', and the lasting period is prolonged to 10-14 days; chitosan oligosaccharide can regulate the midgut environment of pests, and the virus infection rate is increased by 1.8-2.3 times. And the auxiliary agent is compatible with an unmanned aerial vehicle low-volume spraying system and is suitable for green prevention and control of beet armyworms of cruciferous crops such as cabbages, cauliflowers and Chinese cabbages, the control effect reaches 95% or above 7 days after pesticide application, and the lasting period is prolonged by 5-7 days compared with that of a conventional preparation.

The application discloses a production process of culturing nucleotype polyhedrosis virus by using S2 or SF9 cell lines. Cell penetrating peptide C105Y is connected with DSPE-PEG2000-Mal on the surface of a lipid nanoparticle, and then the lipid nanoparticle LNP-C105Y is prepared by using the same, nucleotype polyhedrosis virus DNA is loaded in the lipid nanoparticle, and DNA HcNPV @LNP-C105Y; the DNA HcNPV @LNP-C105Y is inoculated into SF9 Spodoptera frugiperda cell lines or S2 Drosophila cell lines for culture, and nucleotype polyhedrosis virus is produced. The nucleotype polyhedrosis virus of Hyphantria cunea is taken as the basis, a DNA HcNPV @LNP-C105Y lipid nanoparticle is used for wrapping the nucleotype polyhedrosis virus of Hyphantria cunea, and is inoculated into SF9 and S2 cell lines to produce the nucleotype polyhedrosis virus of Hyphantria cunea. The nucleotype polyhedrosis virus production process of the application is improved, and the defect that the existing virus production process cannot be separated from the live feeding of Hyphantria cunea is overcome.

The application discloses a cotton bollworm nuclear polyhedrosis virus spike period targeted synergistic adjuvant, a pesticide preparation and application, relates to the technical field of agricultural biological pesticides, and the composite adjuvant is prepared from ultraviolet protection and shielding materials, film-forming materials, midgut infection promoters, penetration and spreading agents, system compatible components and carriers.The adjuvant is applied by unmanned aerial vehicle top spraying, and after being compounded with HaNPV, the deposition rate of the virus on the corn tassel can be increased by 3.2 times, the retention amount is increased by 5.62 times, the destruction rate of the midgut peritrophic membrane of the cotton bollworm larvae is more than 90%, and the mortality of the 3rd instar cotton bollworm larvae is increased to 87.72%.The application solves the two technical bottlenecks of the virus pesticide, i.e., 'difficult coverage of the tassel target' and 'low midgut infection efficiency', and provides key technical support for green and precise prevention and control of the cotton bollworm in the corn tassel period.

The invention discloses an insecticidal composition containing entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and relates to the technical field of biological insecticide, the active ingredients of the insecticidal composition are entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and the entomopathogenic virus is nuclear polyhedrosis virus of baculoviridae. The weight ratio of the virus to the jasmonic acid methyl ester or jasmonic acid is 1: (0.5-1000), and the insecticidal composition can be prepared into dosage forms such as a suspending agent, water dispersible granules, wettable powder and the like. The invention also provides a method for preventing and treating lepidoptera pests, which comprises the step of applying the entomopathogenic virus and methyl jasmonate or jasmonic acid to target pests or a living environment of the target pests in a combined manner, and the combined application is simultaneous application or successive interval application. The insecticidal composition has a synergistic effect on lepidoptera pests such as cotton bollworms and beet armyworms, can obviously reduce the food intake, accelerate the death of pests and improve the field control effect, and has a wide application prospect.

The present application belongs to the field of bio-technology of agriculture and forestry, and relates to a method for increasing the egg production of Spodoptera litura based on irradiation mode. 60 Co source irradiation treatment; the irradiated Spodoptera litura pupae are cultured in an incubator to emerge, then the adults are moved to an adult rearing cage for rearing, and honey water is provided for nutrition. Through effective accumulated temperature treatment, a large number of Spodoptera litura pupae with consistent development are selected as irradiation objects, which can greatly increase the egg production of Spodoptera litura under the premise of ensuring the emergence rate of Spodoptera litura pupae and the lifespan of adults, increase the production of test insects, and the operation process is simple, safe and reliable, which provides a large amount of production insect source for conveniently and quickly producing biological control agent nuclear polyhedrosis virus by using Spodoptera litura, and expanding the host parasitic wasp by using the insect as host.

The invention relates to the technical field of prevention and control of agricultural and forestry pests, and discloses a synergistic protein ORF76 of insect baculovirus and application of the synergistic protein ORF76. According to the invention, a synergistic protein (ORF76) is obtained from an insect biocontrol resource, i.e., a Pinus fumosa nuclear polyhedrosis virus, and protein function verification shows that the protein has a remarkable synergistic effect on a fall webworm nuclear polyhedrosis virus, an apocheima cinerarius nuclear polyhedrosis virus and a Pinus fumosa nuclear polyhedrosis virus; the compound can be used as a synergistic factor to be added into insect viruses, and has important significance on prevention and control of major forestry pests (fall webworms, spring inchworm and smoke wing pine bees).

The invention discloses an insecticidal composition containing fluchlorantraniliprole and a biogenic insecticide, which comprises an active component A and an active component B. The active component A is selected from fluchlorantraniliprole, the active component B is selected from fluchlorantraniliprole, and the active component C is selected from fluchlorantraniliprole. The active ingredient B is selected from any one of abamectin, spinosad, spinetoram, bacillus thuringiensis, milbemectin, methylamino abamectin, methylamino abamectin benzoate (emamectin benzoate), sucrose octacarboxylate, beauveria bassiana, metarhizium anisopliae, nuclear polyhedrosis virus, cytoplasmic polyhedrosis virus and granulosis virus; the weight ratio of the active component A to the active component B is (1: 80)-(80: 1). The composition disclosed by the invention has a synergistic effect on various pests harmful to agricultural production, reduces the dosage of pesticides, reduces the residual quantity of the pesticides on crops, relieves environmental pollution, is safe to human and livestock, has good environmental compatibility, and delays the generation of pest resistance.

The invention provides a gene with an antiviral effect and application thereof, the nucleotide sequence of an open reading frame of the gene is SEQ ID NO: 2, and the amino acid sequence of a coding protein of the gene is SEQ ID NO: 1. The invention also provides a method for increasing the sensitivity of the beet armyworm to the beet armyworm nuclear polyhedrosis virus, which is used for reducing the expression quantity of the gene in the beet armyworm. According to the invention, a gene with an antiviral effect is screened from an important agricultural pest beet armyworm. The sequence structure and tissue expression mode of the gene are analyzed, incremental expression and RNA interference technologies are utilized to prove that the gene has an anti-SeMNPV effect, a new target is provided for better utilizing baculovirus to prevent and control beet armyworms in production, and the gene has a good application prospect.

The present invention discloses an insecticide composition containing flubendiamide and a biogenic insecticide, wherein the effective active ingredients are active ingredient A and active ingredient B, wherein active ingredient A is selected from flubendiamide, and active ingredient B is selected from any one of the following: avermectin, spinosad, spinetoram, Bacillus thuringiensis, emamectin, emamectin benzoate (emamectin benzoate), sucrose octacarboxylate, Beauveria bassiana, Metarhizium anisopliae, nuclear polyhedrosis virus, cytoplasmic polyhedrosis virus, and granulovirus, and the weight ratio of active ingredient A to active ingredient B is 1:80 to 80:1. The composition of the present invention has a synergistic effect on various pests that harm agricultural production, reduces the amount of pesticide used, reduces the amount of pesticide residues on crops, alleviates environmental pollution, is safe for humans and animals, has good environmental compatibility, and delays the development of pest resistance.

The invention discloses a yellow thorn moth blood lymphocyte line, an expanded production method of a nuclear polyhedrosis virus and application of the yellow thorn moth blood lymphocyte line and the nuclear polyhedrosis virus. The name of the dalbergia mandshurica blood lymphocyte line is dalbergia mandshurica blood lymphocyte CFW-CHY, the dalbergia mandshurica blood lymphocyte line is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: C2025104, and the preservation date is March 27, 2025; the yellow thorn moth blood lymphocyte line is used for expanded production of the nuclear polyhedrosis virus, so that industrial production of the nuclear polyhedrosis virus is realized, and the yellow thorn moth blood lymphocyte line can be used in the fields of preparation of insecticides, insecticidal pesticides and the like.

The present invention discloses the application of the combination of nuclear polyhedrosis virus and Bacillus thuringiensis in the preparation of a preparation for controlling Agrotis ypsilon larvae, and relates to the technical field of controlling Agrotis ypsilon larvae. Through the ratio experiments of nuclear polyhedrosis virus and Bacillus thuringiensis with different types and different concentrations, it is found that, compared with the single use of nuclear polyhedrosis virus or the single use of Bacillus thuringiensis, the combined use of the two has an obvious synergistic effect in the control of Agrotis ypsilon larvae, can significantly increase the mortality rate of Agrotis ypsilon larvae indoors and in the field, improve the control effect, and provides a theoretical basis for the biological control of Agrotis ypsilon. At the same time, the combined use of nuclear polyhedrosis virus and Bacillus thuringiensis can avoid the "3R" problems brought by chemical pesticide control from the root, is environmentally friendly, and is conducive to long-term development.

The present invention relates to synthetic designs or chimeric proteins for pseudotyping baculovirus (Autographa californica nuclear polyhedrosis virus) with the rabies virus glycoprotein G (gG) on its surface (Bac::gG-FL), which can be used in rabies vaccine formulations. The chimeric protein is designed from a gene cassette containing genetic fragments from the ectodomain of the Pasteur variant rabies virus glycoprotein G, a 7-amino-acid connector sequence (GGGGSGG), as well as transmembrane (TM) and cytoplasmic (CT) regions of the baculoviral protein gp64, the arrangement of which in the designed gene cassette is shown in Figure 1.

The present invention belongs to the field of agricultural biotechnology. Specifically, the present invention relates to the application of metformin in the preparation of a drug for preventing or treating Bombyx mori nuclear polyhedrosis virus disease. Through research, it is found that metformin has preventive and therapeutic effects on Bombyx mori infected with nuclear polyhedrosis virus. At the same time, it can also promote the growth and development of Bombyx mori. Metformin has certain biodegradability and is a green and low-pollution biological control agent.

The application belongs to the field of biological pesticides, and provides application of HearNPV-miR-56 in cotton bollworm control, wherein the nucleotide sequence of the HearNPV-miR-56 is CGGUAACAGAAGCGGUCACG. The HearNPV-miR-56 can improve virus replication amount of cotton bollworm nuclear polyhedrosis virus infected larvae, thereby promoting death, and can be used for developing a cotton bollworm nuclear polyhedrosis virus synergist, and has potential application prospect in biological control of cotton bollworm.

The present invention discloses a method for producing chimeric silk using silkworms by using Autographa californica nuclear polyhedrosis virus, and constructs a chimeric silk containing FibL-MaSp-c-polyA FibL The plasmid pFAST‑FibL‑MaSp‑c of the fragment is transformed into Escherichia coli containing AcBacmid DH10Ac, and recombinant AcBacmid‑FibL‑MaSp‑c is obtained by screening. The recombinant virus AcNPV‑FibL‑MaSp‑c is transfected into Sf‑9 cells to obtain the recombinant virus AcNPV‑FibL‑MaSp‑c, which is inoculated into 5-instar silkworm larvae, and the larvae are fed with cool-dried fresh mulberry leaves soaked or sprayed with antibiotic liquid for one day, and then fed with fresh mulberry leaves until they mature. The mature silkworms are fed with fresh mulberry leaves soaked or sprayed with ecdysteroid liquid; or the mature silkworms are directly sprayed with ecdysteroid liquid, and the mature silkworms are moved to a cocooning machine, cocoons are harvested after cocooning, and the cocoons are dried and reeled to obtain chimeric silk, thereby meeting the demand for diversity of silk protein in the preparation of various biomaterials.

The application discloses application of apolipoprotein III in prevention and treatment of Bombyx mori nuclear polyhedrosis virus infection and belongs to the cross field of molecular biology technology field and virus prevention and treatment. The application first finds that Bombyx mori apolipoprotein III (ApoLp-III) promotes biosynthesis and accumulation of ceramide, thereby inhibits mTORC1 signal pathway activity, induces cell G1 phase arrest, and effectively blocks a new mechanism of Bombyx mori nuclear polyhedrosis virus (BmNPV) replication. Based on the mechanism, the application provides application of ApoLp-III in inhibition of BmNPV proliferation, prevention and treatment of BmNPV infection, improvement of the ability of Bombyx mori to resist BmNPV or cultivation of a BmNPV-resistant Bombyx mori strain. The application provides a brand-new technical strategy and germplasm resource for realizing green, efficient and sustainable prevention and control of BmNPV.

This invention discloses the application of cucurbitacin B in the preparation of drugs for the treatment or prevention of silkworm nucleopolyhedrovirus. The research of this invention shows that the terpene compound cucurbitacin B can effectively inhibit the replication of silkworm nucleopolyhedrovirus (BmNPV) in silkworm cells and within the silkworm. By using appropriate concentrations and drug addition times, cucurbitacin B can inhibit the replication of the virus in silkworm cells and within the silkworm, providing a new approach for the prevention of BmNPV infection in silkworms and laying the foundation for the prevention and control of viral infections in sericulture.

The invention discloses a method for improving antibacterial peptide Attactin secreted by bombyx mori, and provides application of # imgabs0 # gene as a regulation target or a regulation marker in improving antibacterial peptide Attactin secreted by bombyx mori for the first time, the method comprises the following steps: injecting an RNAi interference fragment of # imgabs1 # gene into five-year-old larvae on the first day of bombyx mori to continuously infect bombyx mori nuclear polyhedrosis virus after 6 hours; the antibacterial peptide Attactin expressed by bombyx mori can be effectively improved, the death time of bombyx mori infected with nuclear polyhedrosis virus can be prolonged, a new thought can be provided for improving the expression quantity of the antibacterial peptide by using # imgabs2 # and the like as molecular targets, and a pathogen resisting mechanism of bombyx mori can be deeply understood; reference is provided for bombyx mori antiviral research, and valuable reference is provided for deep research of the effect of # imgabs3 # in bombyx mori immune reaction.

The invention discloses a small-particle-size hyphantria cunea nuclear polyhedrosis virus nano-emulsion as well as a preparation method and application thereof. Improvement is carried out on the basis of hyphantria cunea nuclear polyhedrosis virus, nano material preparation and Pickering emulsification technologies are combined, natural renewable polysaccharide polymer sodium alginate is used as a raw material, a sodium alginate derivative (CSAD) is obtained through cholesterol grafting modification, a stable Pickering emulsion is formed after the nuclear polyhedrosis virus is entrapped, and the stable Pickering emulsion is prepared. The novel dosage form has nanoscale particle diameter and is suitable for being used under low volume, the fast-acting property of the dosage form on the fall webworm is remarkably improved, the insecticidal activity is based on natural virus particles of the fall webworm, and the novel dosage form has specificity on target pests and is small in environmental influence. According to the method, the used materials are low in price, the operation process is simple and easy to standardize, and the nano Pickering emulsion has better persistence compared with a common aqueous solution, and is convenient to use and easy to popularize.

The invention discloses a fluorescent RAA primer group, a kit, application and a method for detecting common pathogen beauveria bassiana of bombyx mori, the kit comprises a specific primer group Bb-F / Bb-R, a probe Bb-P and a targeted beauveria bassiana ITS conserved region, during operation, only sample DNA needs to be extracted, reaction is performed for 30 minutes under the constant temperature condition of 42 DEG C, and a result is interpreted in real time through a fluorescent signal. The kit is high in specificity, only amplifies beauveria bassiana DNA, has no cross reaction on common silkworm pathogens such as silkworm nuclear polyhedrosis virus and silkworm microsporidia and healthy silkworm tissues, and also has no cross reaction on other fungal pathogens of silkworms; the sensitivity is high, and the lowest detection line is 3.91 * 10 copies / mu L; the method has the advantages of simple operation, fast detection, completion within 30 minutes, no need of complex equipment, suitableness for the fields of silkworm white muscardine silkworm early detection, epidemic situation monitoring, silkworm larva medicinal material inspection and the like, provides an efficient and accurate molecular diagnosis tool for silkworm white muscardine silkworm prevention and control and silkworm larva identification, and is especially suitable for being popularized and used in grassroots.

The invention relates to a protein for improving the yield of a nuclear polyhedrosis virus and application of the protein. The amino acid sequence of the protein is shown as SEQ ID No. 1 or SEQ ID No. 5. Under the condition of producing the same amount of the nuclear polyhedrosis virus, the production cost of the nuclear polyhedrosis virus as a biopesticide can be greatly reduced by using the two proteins.

Disclosed is a silk gland recombinant expression vector of silkworm expressing human epidermal growth factor, a preparation method and use thereof. The recombinant expression vector is formed by fusing truncated silk fibroin light chain N-terminal domain, C-terminal domain, optimized human epidermal growth factor and silkworm nuclear polyhedrosis virus enhancer, and removes the redundant part of the extra domain of endogenous silk protein contained in the fusion protein to the maximum extent.

The invention discloses a method for preventing and controlling prodenia litura nuclear polyhedrosis virus diseases, and belongs to the technical field of large-scale breeding of insects. The method aims at solving the problems that in the prior art, the chemical disinfection effect is limited, and the disease resistance of prodenia litura is difficult to improve. According to the technical scheme, the method mainly comprises the following steps: firstly, disinfecting prodenia litura eggs to reduce the initial virus load; secondly, an immunopotentiator is added into the basal feed, and the immunopotentiator is selected from sodium houttuyfonate, houttuynia cordata powder or a boiling soak solution prepared from houttuynia cordata roots, stems and leaves, so that the immunologic function of the prodenia litura is enhanced, and the resistance of the prodenia litura to nuclear polyhedrosis virus (NPV) is improved. Through verification, the survival rate of prodenia litura infected with NPV in large-scale feeding can be remarkably increased, and the method is easy and convenient to operate, low in cost and environmentally friendly.

Disclosed is a silk gland recombinant expression vector of silkworm expressing human epidermal growth factor, a preparation method and use thereof. The recombinant expression vector is formed by fusing truncated silk fibroin light chain N-terminal domain, C-terminal domain, optimized human epidermal growth factor and silkworm nuclear polyhedrosis virus enhancer, and removes the redundant part of the extra domain of endogenous silk protein contained in the fusion protein to the maximum extent.