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Antinuclear polyhedron virus transgenic silkworm and generating method thereof

A karyotype polyhedron and transgenic technology, applied in the direction of antiviral agents, medical raw materials derived from viruses/phages, and cells modified by introducing foreign genetic material, etc. And other issues

Inactive Publication Date: 2005-12-14
NAT INST OF AGROBIOLOGICAL SCI
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  • Abstract
  • Description
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Problems solved by technology

However, such an approach in a silkworm farm is not only troublesome, but also causes a huge consumption of manpower and material resources, and the cost of cocooning is expensive, and it has become one of the factors that complicate the farm

Method used

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  • Antinuclear polyhedron virus transgenic silkworm and generating method thereof
  • Antinuclear polyhedron virus transgenic silkworm and generating method thereof

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Embodiment

[0083]The present invention can be explained in detail with reference to the following examples; however, it is not limited thereto.

[0084] In order to inhibit the growth of the virus, a gene derived from the virus is used to recombinantly produce a gene that can produce mRNA with a hairpin structure. The structures of the produced genes and vectors are shown in figure 1 middle.

[0085] The lef1 gene is a gene related to the DNA replication of NPV in silkworm, and also a gene necessary for virus propagation. The gene was ligated, wherein the direction of transcription of the gene was reversed, and SK18, which exhibited strong activity in cultured cells, was used as its promoter. Moreover, the A3GFP gene can be used as a marker gene to distinguish transgenic silkworms carrying the gene. A3GFP can express fluorescent protein in the whole silkworm body, and individuals carrying the gene can be easily distinguished under a fluorescent stereoscopic microscope.

[0086] The ve...

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Abstract

The object of the present invention is to provide a transgenic silkworm exhibiting resistance to NPV and a method for its production. Transgenic silkworms carrying expression DNA encoding an RNA molecule that showed an RNAi effect against genes essential for NPV proliferation were produced. Studies of NPV proliferation in this transgenic silkworm showed that the proliferation of these viruses can be inhibited.

Description

technical field [0001] The invention relates to a transgenic silkworm resistant to nuclear polyhedrosis virus. Furthermore, the present invention relates to a method for producing transgenic silkworms resistant to nuclear polyhedrosis virus. Background technique [0002] In sericulture factories, silkworms are severely damaged by nuclear polyhedrosis virus (NPV). So far, many researchers have carried out the research work of producing anti-NPV silkworms, but none of them has been successful so far. This is due to the fact that the existing silkworms in the world lack the anti-NPV gene. In order to prevent the invasion of this virus, thorough disinfection of silkworm egg hatching areas and related equipment is encouraged. However, such an approach in a sericulture farm is not only troublesome, but also causes huge consumption of manpower and material resources, and cocooning is expensive, and has become one of the factors that complicate the farm. [0003] NPVs are large ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/09A61K35/76A61K36/06A61P31/12C12N5/10C12N15/113
Inventor 田村俊树小岛桂神田俊男伴户久德松井英雄町田顺一桑原伸夫
Owner NAT INST OF AGROBIOLOGICAL SCI
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