Method for select-breeding anti-virosis Bombyx mori varieties by utilizing Thailand Bombyx mori disease-resistant gene

Abstract

The invention discloses a method for select-breeding anti-virosis Bombyx mori varieties by using Thailand Bombyx mori disease-resistant gene. The method comprises the steps of: selecting the present Bombyx mori varieties of 871 and 872 used both in spring and autumn in China as female parent and using the introduced Thailand tropical disease-resistant variety NL as male parent; respectively hybridizing and leading-in the disease-resistance gene; hybridizing second generation and third generation silkworm and respectively adopting mixed batches rearing for breeding; hybridizing each generation beginning from a second age and screening by adopting 'population feeding' NPV (nuclear polyhedrosis virus) and a method with the concentration of virus feeding 'increasing age by age in a gradient way'; taking the Bombyx mori varieties 871 and 872 as recurrent parent, respectively adopting reserve seed antivirus silkworm individual to backcross, using a method of the combination of 'backcrossing introduction' and 'generation directed virus feeding screening breeding' to obtain an NL pure and mild system with comparatively stable genetic character by a plurality of backcrosses and virus feeding screening. In the process of select-breeding, various economic characters and production properties of the system are consolidated and improved and the objects of pure breeds of 871NL and 872NL are bred. The obtained antivirus Bombyx mori variety has good economic characters, is sturdy and easy to keep.

Description

Technical field: [0001] The invention relates to a method for breeding silkworm varieties resistant to viral diseases, and belongs to the field of cultivating and utilizing fine varieties of crops. Background technique: [0002] The world's silk science and technology developed countries should first promote Japan. Since 1998, due to the limitation of labor force and land resources, Japanese silk production has gradually shrunk, and conventional silk production has been no longer studied, but has turned to basic biological research. my country is the birthplace of the silk industry. Sericulture is an important part of my country's rural economy at present. There are more than 20 million households who plant mulberry and sericulture in the country, covering more than 20 provinces and autonomous regions. Silkworm cocoon and raw silk production account for three-quarters of the world's total output, silk fabric production accounts for more than two-thirds of the world's total...

AI Technical Summary

Problems solved by technology

Since the first national disease resistance breeding conference in my country in 1981, relevant scientific research, teaching and production units across the country have carried out a general survey on the resistance of silkworms to major silkworm diseases and stress resistance to the environment, etc., and the genetic rules of resistance and their resistance A lot of research has been done on variety selection, etc. Some progress has been made in disease resistance mechanism, genetic analysis of disease resistance traits, and differences between varieties, but there is still no silkworm variety with better disease resistance, especially NPV resistance. The variety has not been able to break through, the main misunderstanding is that the silkworm disease-resistant gene has not been obtained, and the selection and promotion of silkworm disease-resistant varieties are still in the initial stage of research

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