Method for preventing and treating vegetable noctuid using attractant for spreading virus
An attractant and virus technology, applied in the field of vegetable pest control, can solve the problems of pesticide resistance of harmful organisms, long residual effect period of pesticides, large amount of virus pesticides, etc. natural pop effect
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[0020] a. Material selection
[0021] Nuclear polyhedrosis virus: take 10 grams of dead moth pests and add 3 times of sterile water to dilute, grind twice with a mortar, filter with 2 layers of gauze, and take the filtrate for later use; take out the filtrate and dilute with distilled water at 1:10, The mixture was first centrifuged at 3200r / min for 30 minutes, the supernatant was discarded, and the precipitate was completely suspended by adding 0.1% neutral detergent, centrifuged at a low speed of 400r / min for 4 minutes, the supernatant was taken, washed and centrifuged twice to obtain The pure nuclear polyhedrosis virus is fixed to volume with 1000ml of water to obtain mother liquor and made into suspoemulsion for later use.
[0022] Sex lure: Use noctuid moth attractant lure core.
[0023] b. Virus transmission
[0024] Carry out in plastic green vegetable greenhouses, choose the water basin trap produced by Zhejiang Ningbo Newcomb Biotechnology Co., Ltd.; dilute the moth...
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