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Ectropis oblique warren nuclear polyhedrosis virus insecticidal suspending agent and production method thereof

A virus insecticide and a production method technology, which are applied in the field of tea inchworm nuclear polyhedrosis virus insecticidal suspension agent to achieve the effects of avoiding the enhancement of insect resistance, low control cost and environmental friendliness

Inactive Publication Date: 2015-03-25
INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The above inventions all focus on the artificial feed for tea geometrid larvae and the artificial feeding and subculture of tea geometrid larvae, but the indoor artificial large-scale propagation of tea geometrid nuclear polyhedrosis virus and the processing technology of tea geometrid virus insecticide are not involved.

Method used

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  • Ectropis oblique warren nuclear polyhedrosis virus insecticidal suspending agent and production method thereof

Examples

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Effect test

Embodiment 1

[0030] Embodiment 1. 5000 tea geometrid pupae were placed in an incubator at 24° C., and eclosion began after 10 days, and 2 pairs of adults were put into each egg-laying container. The artificial diet for tea geometrid larvae was evenly heated according to formula 1 in Table 1, and then packed into tea geometrid larva feeding plates, and the newly hatched larvae were inserted into the healthy insect breeding room to raise them to the 4th instar at a room temperature of 24°C. The feed is divided into the tea geometrid larva feeding plate, and the surface is connected with a virus suspension with a concentration of 2 million PIB / ml. After 10 days, the corpses of dead insects caused by viral diseases were collected and processed into a virus suspension of 20 billion PIB / ml.

[0031]Then, according to the weight ratio, 15% of 20 billion PIB / ml virus suspension, 0.2% of 60nm silicon dioxide, 0.1% of aromatic amino acid, 0.5% of refined fluorescent whitening agent, 0.2% of sodium l...

Embodiment 2

[0032] Example 2, 10,000 tea geometrid pupae were placed in an incubator at 24°C, and eclosion began after 10 days, and 2 pairs of adults were placed in each egg-laying container. The artificial feed for tea geometrid larvae was evenly heated according to formula 2 in Table 1, and then packed into tea geometrid larvae feeding plates, and the newly hatched larvae were inserted into the healthy insect breeding room to raise them to the 4th instar at a room temperature of 24°C. The feed is divided into the tea geometrid larva feeding plate, and the surface is connected with a virus suspension with a concentration of 1 million PIB / ml. After 10 days, the corpses of dead insects caused by viral diseases were collected and processed into a virus suspension of 20 billion PIB / ml.

[0033] Then, according to the weight ratio, 15% of 20 billion PIB / ml virus suspension, 0.3% of 60nm silicon dioxide, 0.13% of aromatic amino acid, 0.8% of refined fluorescent whitening agent, 0.4% of sodium ...

Embodiment 3

[0034] Example 3, 15,000 tea geometrid pupae were placed in an incubator at 24°C, and eclosion began after 10 days, and 2 pairs of adults were placed in each egg-laying container. The artificial diet for tea geometrid larvae was evenly heated according to formula 3 in Table 1, and then packed into tea geometrid larvae feeding plates, and the newly hatched larvae were inserted into the healthy insect breeding room to raise them to the 4th instar at a room temperature of 24°C. The feed is divided into the tea geometrid larva feeding plate, and the surface is connected with a virus suspension with a concentration of 1 million PIB / ml. After 10 days, the corpses of dead insects caused by viral diseases were collected and processed into a virus suspension of 20 billion PIB / ml.

[0035] Then, according to the weight ratio, 15% of 20 billion PIB / ml virus suspension, 0.4% of 60nm silicon dioxide, 0.14% of aromatic amino acid, 0.8% of refined fluorescent whitening agent, 0.6% of sodium ...

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Abstract

The invention mainly aims to provide an ectropis oblique warren nuclear polyhedrosis virus insecticidal suspending agent and a production method thereof. The insecticide consists of virus suspension, silicon dioxide, aromatic amino acids, a refined fluorescent whitening agent, sodium lignin sulfonate, polyvinyl alcohol, chlorfluazuron, glycerin and sterile water. The production method comprises the following steps: paired spawning on pupas by virtue of emerged adults, sterilizing the eggs, hatching larvae, feeding the larvae by using artificial feed, inoculating ectropis oblique warren virus on the feed surface to feed, grinding and filtering the larvae infected with the virus, centrifuging filtrate, collecting precipitates, and preparing the corresponding insecticide. By utilizing the insecticide, plant diseases and insect pests of ectropis oblique warren can be effectively controlled, the control cost is low, the environment friendliness is promoted, and the problems that the drug resistance of injurious insects is enhanced, the natural enemy number is reduced and pesticide residues in tea leaves are increased due to use of lots of chemical pesticides are solved.

Description

technical field [0001] The invention relates to biological insecticides, in particular to a tea geometrid nucleopolyhedrosis virus insecticide suspension and a production method of the virus insecticide suspension. Background technique [0002] Tea geometrid (Ectropis oblique Warren), also known as arch worm, measure worm, hanging silkworm, is mainly distributed in Anhui, Jiangsu, Zhejiang, Jiangxi, Hubei, Hunan, Sichuan, Guangdong and Fujian provinces. It is the main pest of tea tree, which belongs to Geometridae of Lepidoptera. The larvae eat leaves, and when an outbreak occurs, the entire tea garden can be eaten into polished rods, resulting in a reduction in tea yield of more than 60%, which seriously affects the quality of finished tea. [0003] In the past, the basic methods of controlling tea inchworms were strengthening forecasting and agricultural control, manual hunting, and scientific use of chemical pesticides. However, long-term use of chemical pesticides in l...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01N25/04A01P7/04
Inventor 靳亮王洪秀王金昌张莉莉关丽梅郑国华黄朝
Owner INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
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