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Hyphantria cunea viral insecticide and preparation method thereof

An American white moth virus and technology of American white moth, applied in the direction of insecticides, botanical equipment and methods, biocides, etc., can solve the problems that are not involved in dosage form design and processing technology, and achieve prolonged virus duration and good Protective effect, good suspension effect

Inactive Publication Date: 2015-03-25
INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, neither the dosage form design nor the processing technology of the American white moth virus insecticide involved

Method used

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  • Hyphantria cunea viral insecticide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 5,000 white moth pupae were placed in an incubator at 25°C, and eclosion began after 10 days, and 2 pairs of adults were placed in each egg-laying container. The artificial diet for white moth larvae was evenly heated according to formula 1 in Table 1, and then packed into the feeding boards for white moth larvae, and the newly hatched larvae were inserted into the healthy insect breeding room to raise them to the 4th instar at a room temperature of 25°C. The feed is divided into the feeding plate of white moth larvae, and the surface is connected with a virus suspension with a concentration of 1 million PIB / ml. After 10 days, the corpses of dead insects caused by viral diseases were collected and processed into a virus precipitate of 20 billion PIB / ml.

[0024] Then, according to the weight ratio, 15% of 20 billion PIB / ml virus precipitate, 0.3% of 60nm silicon dioxide, 0.12% of aromatic amino acid, 1.0% of refined fluorescent whitening agent, 0.2% of sodium ...

Embodiment 2

[0025] Example 2 10,000 pupae of the American white moth were placed in an incubator at 25°C, and eclosion began after 10 days, and 2 pairs of adults were placed in each egg-laying container. The artificial diet for white moth larvae was evenly heated according to formula 2 in Table 1, and then packed into the feeding boards for white moth larvae. The newly hatched larvae were inserted into the healthy insect breeding room and raised to the 4th instar at a room temperature of 25°C. The feed is divided into the feeding plate of white moth larvae, and the surface is connected with a virus suspension with a concentration of 1 million PIB / ml. After 10 days, the corpses of dead insects caused by viral diseases were collected and processed into a virus precipitate of 20 billion PIB / ml.

[0026] Then, according to the weight ratio, 10% of 20 billion PIB / ml virus precipitate, 0.2% of 60nm silicon dioxide, 0.14% of aromatic amino acid, 0.8% of refined fluorescent whitening agent, 0.3% ...

Embodiment 3

[0027] Example 3 20,000 white moth pupae were placed in an incubator at 25°C, and eclosion began after 10 days, and 2 pairs of adults were placed in each egg-laying container. The artificial diet for white moth larvae was evenly heated according to formula 3 in Table 1, and then packed into the feeding boards for white moth larvae, and the newly hatched larvae were inserted into the healthy insect breeding room to raise them to the 4th instar at a room temperature of 25°C. The feed is divided into the feeding plate of white moth larvae, and the surface is connected with a virus suspension with a concentration of 1 million PIB / ml. After 10 days, the corpses of dead insects caused by viral diseases were collected and processed into a virus precipitate of 20 billion PIB / ml.

[0028] Then, according to the weight ratio, add 20 billion PIB / ml virus precipitate 8%, 60nm silicon dioxide 0.15%, aromatic amino acid 0.14%, refined fluorescent whitening agent 0.8%, sodium lignosulfonate ...

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Abstract

The invention provides a hyphantria cunea viral insecticide and a preparation method thereof. The preparation method comprises the following steps: feeding hyphantria cunea by virtue of artificial feed, preparing a nuclear polyhedrosis virus precipitate, and adding trace inorganic nano-materials, aromatic amino acids and a small amount of refined fluorescent whitening agent into the virus precipitate. According to the preparation method, the viral insecticide is complete in components and high in suspension property, the quality guarantee period is prolonged, the insecticide has a good protective effect against ultraviolet light during field application, the virus duration is long, and the control effect is greatly improved.

Description

technical field [0001] The invention relates to the field of biological insecticides, in particular to an insecticidal suspension of white moth nuclear polyhedrosis virus. The invention also relates to a preparation method for preparing the American white moth nuclear polyhedrosis virus insecticidal suspension. Background technique [0002] Since the American white moth was discovered in Liaoning Province in 1979, the epidemic has spread in many provinces and cities such as Tianjin, Beijing, Hebei and Shandong. The larvae of the American white moth can reproduce for 2-3 generations a year in the north. They have the characteristics of miscellaneous food, strong adaptability, large reproduction, wide transmission routes, and serious damage. They will cause direct harm to forest fruits, crops and green plants. [0003] Traditional American white moth control is mainly chemical pesticides. Chemical pesticides have the characteristics of high efficiency and rapidity in the ear...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01N25/04A01N25/22A01P7/04
Inventor 靳亮王洪秀陈晓燕王金昌张莉莉黄朝
Owner INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
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