The invention provides a process for producing a virus biological insect-proofing organic fertilizer, which relates to the technical field of biological treatment of livestock excrements and life sewage. The process employs the following steps: preparing a raw material, propagating insect virus, collecting the insect virus and the organic fertilizer raw material, mixing, drying, crushing, examining and packaging for warehousing. The process for producing a virus biological insect-proofing organic fertilizer can effectively prevent and treat soil insects, such as cutworm, wireworm, Chinese mole cricket, grub, vegetable and fruit root-knot nematode disease, rice-stem borer and yellow rice borer, so that an organic matter can be increased, the soil granule structure is improved, the soil fertility can be improved by culturing fertilizer, the crops quality is improved, the unit area output is increased, and the ecological balance can be recovered.