Preparation for ultraviolet-resistant inactivating insect virus microcapsule

An insect virus, anti-ultraviolet technology

Inactive Publication Date: 2018-02-23
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the technical problems of existing chemical pesticides such as high cost, poor drug efficacy, and serious pollution, and provide a method for preparing a green, non-residue, and environmentally friendly anti-ultraviolet inactivation insect virus preparation

Method used

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  • Preparation for ultraviolet-resistant inactivating insect virus microcapsule
  • Preparation for ultraviolet-resistant inactivating insect virus microcapsule

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specific Embodiment approach 1

[0018] Specific embodiment one: the preparation method of anti-ultraviolet inactivation insect virus preparation in the present embodiment is carried out according to the following steps:

[0019] (1), preparation of chitosan-anti-white moth virus complex mixed solution: add low-viscosity chitosan into deionized water according to the concentration of 2%, stir at medium speed with a magnetic stirrer, and add Acetic acid is used to adjust the pH of the solution to about 4.8 until the solution is clarified and the chitosan is completely dissolved, then the anti-white moth virus complex is added, the mass ratio of the virus complex to chitosan is 1: 4, and the mixture is stirred at a high speed for 10 minutes at room temperature and protected from light. ~30min to obtain a uniformly dispersed chitosan-virus complex mixed solution;

[0020] (2), preparation of oil phase: the volume ratio of the volume of deionized water and n-hexane is 1:8 to measure n-hexane, stearic acid is adde...

specific Embodiment approach 2

[0022] Specific embodiment two: the preparation method of anti-ultraviolet inactivation insect virus preparation in the present embodiment is carried out according to the following steps:

[0023] (1), preparation of virus complex solution: add the insect virus complex to deionized water according to the volume ratio of the mass of the anti-white moth virus complex and deionized water to 0.1g: 2ml, and use a magnetic stirrer at a medium speed Stir for 30 minutes until the virus is completely dispersed.

[0024] (2), preparation of chitosan-anti-white moth virus complex mixed solution: add low-viscosity chitosan to deionized water according to the concentration of 2%, stir at medium speed with a magnetic stirrer, and add in the stirring process Acetic acid was used to adjust the pH of the solution to about 4.8 until the chitosan was completely dissolved when the solution was clear, then the anti-white moth virus complex solution was added dropwise to the chitosan solution at a ...

specific Embodiment approach 3

[0027] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that: the sediment obtained by centrifugation described in step three is washed three times with petroleum ether. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

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Abstract

The invention relates to a preparation of an ultraviolet-resistant inactivating insect virus and belongs to the field of green agricultural biological prevention and control. The invention aims to solve the technical problems that the present insect virus is easy to inactivate at outside and poor in stability. The preparation is realized according to the following method: 1) preparing chitosan-virus mixed solution; 2) preparing an oil phase; and 3) biologically preparing a chitosan microcapsule. The ultraviolet-resistant inactivating insect virus according to the invention is capable of reducing the environmental pollution, promoting the use stability and reducing the production cost, and meanwhile, is capable of effectively prolonging the pesticide effect duration of the insect virus andis applicable to the fields of control on crop growth, crop pest killing, and the like.

Description

technical field [0001] The invention relates to a preparation method of a green, non-residue and environment-friendly anti-ultraviolet inactivation insect virus preparation. Background technique [0002] Currently, about 2 million tons of pesticides are used every year worldwide to control pathogens and pests. However, it is estimated that about 90% of pesticides are lost due to degradation, photolysis, evaporation and surface runoff, and only 0.1% of pesticides can finally be deposited on harmful biological targets to play a role. This long-term widespread and ineffective use of pesticides has caused serious pollution to the ecological environment. Societal concerns motivate researchers to develop effective, safe and green pesticide formulations. Viral biopesticide is a pure natural microbial insecticide that is specific, long-lasting, not easy to produce resistance, and safe. The use of biopesticides to replace chemical pesticides is the trend of international pesticide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01N25/28A01P7/04
CPCA01N25/28A01N63/00
Inventor 梁俊王静曲良建
Owner TIANJIN UNIV OF SCI & TECH
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