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Method for increasing insect virus propagation efficiency

An insect virus and efficiency technology, which is applied in the field of improving the multiplication efficiency of insect viruses, can solve problems such as poor control effect and reduced mortality rate, and achieve the effects of reducing pupation rate, good control effect and improving insecticidal activity.

Inactive Publication Date: 2003-03-12
环球生物农药有限公司
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AI Technical Summary

Problems solved by technology

With the increase of instar age, the disease resistance of larvae becomes stronger and stronger, the mortality rate decreases, and the control effect becomes worse

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] 1. Preparation of insect virus synergistic protein

[0026] (1) Isolation and purification of insect virus synergistic protein

[0027] Preparation of synergistic proteins of insect viruses such as Trichoplusia granulosa virus: artificially infect insect larvae with insect virus, collect dead larvae, homogenate, and differentially centrifuge 3 times to obtain relatively pure polyhedron. After centrifugation with 30-60% (w / w) sucrose density gradient, the polyhedron band is taken out, and the sucrose is washed away to obtain pure polyhedron. Make the precipitate into 1.0×10 9 PIB / mL of virus stock solution. Adjust the inclusion body of insect virus to 2.0 × 10 9 PIB / ml, with an equal volume of 0.1mol / LNa 2 CO 3 Mix -0.17mol / L NaCl solution thoroughly, act at 4°C for 50 minutes, keep warm at 37°C until the liquid turns from turbid to clear, stop the reaction, and adjust the solution to pH 7.0 with 1mol / L HCl.

[0028] The synergistic protein was isolated and purifie...

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Abstract

A process for increasing the reproduction efficiency of insect virus is characterized by that the synergistic protein of insect virus and the inclusion body of insect virus are exploded to obtain virus particles, which are used to infect the adult insects for high-efficiency and high-level reproduction of said virus in the insects.

Description

technical field [0001] The present invention relates to the proliferation of insect viruses and their susceptibility to insect larvae. Specifically, it relates to a method for improving the effective multiplication of insect viruses by using virus particles obtained by blasting technology in vitro from insect virus synergistic proteins and insect virus inclusion bodies; it also involves improving the sensitivity of insect larvae to insect viruses and improving insect Methods of viral insecticide activity. Background technique [0002] In 1956, Tanada et al. isolated the synergistic protein from Pseudaletia unipuncta granular virus (PuGV) for the first time, and confirmed that the synergistic protein was effective against Pseudaletia unipuncta nuclear polyhedrosis virus (Pseudaletia unipuncta nuclear polyhedrosis virus, PuNPV) has the effect of enhancing infection (Tanada Y et al., J Invertebr Pathol, 1959, 1: 215-231). Hashimoto (Hashimoto Y et al., J Gen Virol, 1991, 72: ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00C12N7/00C12N7/01C12N15/86
Inventor 孟小林徐进平王健鲁伟
Owner 环球生物农药有限公司
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