Synergistic method of insect growth regulator and nucleopolyhedron virus
A nuclear polyhedrosis virus and growth regulator technology, applied in plant growth regulators, chemicals for biological control, biocides, etc., can solve the problem of reducing the level of biological control of pests, slow action of insect viruses, and death of pests and other issues to achieve the effect of increasing the effect of harm control, improving the effect of harm control and reducing environmental pollution
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Embodiment 1
[0025] Embodiment 1: Chlorfluazuron and SlNPV mixed use
[0026] Indoor bioassays showed (Table 3) that the mixed use of chlorfluazuron and SlNPV had a significant synergistic effect. The co-toxicity coefficients of the ratios A, B, C, D and E were 137, 162, 182, 124 and 157, respectively, all showing synergistic effects.
[0027] Chlorfluazuron: SlNPV
Virulence regression equation
LC 50 (mg / L)
r value
toxicity index
co-toxicity coefficient
3:1(A)
2:1 (B)
1:1(C)
1:2(D)
1:3(E)
SlNPV
Chlorfluazuron
Y=2.5039+0.9028X
Y=1.9627+1.1619X
Y=1.9860+1.2350X
Y=2.4351+1.0217X
Y=2.3153+1.1342X
Y=2.3290+1.0855X
Y=-3.0120+2.4419X
581.74
411.32
275.76
323.82
232.91
288.84
1909.90
0.9667
0.9495
0.9588
0.9614
0.9605
0.9852
0.9465
3.28
4.64
6.93
5.90
8.20
6.61
1.00
137
162
182
124
157
...
Embodiment 2
[0033] Embodiment 2: mixed use of chlorfluazuron and AcNPV
[0034] Indoor bioassays on beet armyworm showed (Table 6) that the mixed use of chlorfluazuron and AcNPV also had a synergistic effect. The co-toxicity coefficients of the ratios A, B, C, D and E were 127, 155, 153, 109 and 130, respectively, all showing synergistic effects.
[0035] Chlorfluazuron: AcNPV
[0036]From the results of field experiments in Nanjing and Rugao, it can be seen that the combination of AcNPV and chlorfluazuron has a good control effect on beet armyworm. The field test in 2002 showed (table 7), 3 days after the medicine, mu uses virus single agent 4 * 10 9 The control effect of PIB on beet armyworm was 18%, while AcNPV 10 9 PIB plus chlorfluazuron 0.4 g, AcNPV 2×10 9 PIB plus chlorfluazuron 0.6 g, AcNPV 4×10 9 The control effects of PIB plus chlorfluazuron 0.8 g were 30.8%, 49.5% and 50.8%, respectively, which were significantly higher than the control effects of a single agent o...
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