The invention relates to the technical field of a preparation method of a determination kit for in-vitro determination of
adiponectin in a
human body, and particularly discloses a preparation method of a determination kit for in-vitro determination of
adiponectin in a
human body, which comprises the following steps: a
coupling process; an S-shaped bent
pipe is installed on the side connector, an upper connector is kept in an open state, a
perfusion device adds 0.3-0.4 volume of a sealing
buffer solution into the glass
bottle, and 0.1-0.2 volume of the sealing
buffer solution is injected into the glass
bottle through an injection connector; and a storage process: closing the filling interface, opening the cover body, installing the up-down extending
pipe, covering the cover body, opening the filling interface, carrying out resuspension ultrasonic treatment by using the storage
buffer solution, and carrying out centrifugal washing for three times, thereby finally obtaining the
adiponectin latex
antibody. The device is easy and convenient to operate, simple in structure, independent of the manual
control level and high in preparation efficiency, the situation that materials are left in a small-size glass
bottle, consequently, the material ratio is difficult to control, and a small amount of confining liquid is needed is avoided, and the prepared adiponectin latex
antibody is good in accuracy.