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Collecting method for insect virus breed

An insect virus and virus technology, applied in the directions of viruses/phages, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problem of labor-intensive and time-consuming virus collection, difficulty in identifying dying larvae, and loss of virus yield, etc. problem, to achieve the effect of simplified method, easy operation, and improved virus yield

Inactive Publication Date: 2005-02-23
TEA RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such as tea inchworm, the output is only 100-150 million polyhedron (PIB) per head, the work of virus collection is labor-intensive and time-consuming, and the operation is very cumbersome. For example, the general larvae start to die after 7-8 days of poisoning, that is, the corpses of the insects need to be collected every day With dying larvae, it takes about 7-8 days to collect, and the whole process of poisoning, multiplication and collection takes 15-16 days to end. The corpses are broken during collection, the virus yield is lost, and it is difficult to confirm the collection of dying larvae. Therefore, how to improve the yield of single insect virus and simplify the collection method in insect virus propagation is an urgent technical problem to be solved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1 Production process of tea looper virus

[0014] (1) Feeding of larvae

[0015] 1. One pair of adults (female, male) are raised in a glass can (glass container), and the adults are taken out after laying eggs.

[0016] 2. When the above eggs hatch into larvae, they should be fed with feed (tea or artificial feed), and feed should be added before the feed is finished. As the larvae continue to grow, the larvae in one bottle are divided into 3 or more bottles, and finally the number of larvae in each bottle is about 50. Feeding with artificial feed should be divided into several bottles, 10-15 heads per bottle.

[0017] (2) Virus reproduction (multiplication)

[0018] 3. When the above-mentioned larvae are reared at 24°C for 7-8 days, add poisonous leaves (the leaves are soaked in a 005 million polyhedron / ml virus solution and then taken out and dried) to feed poison, and then reared with non-toxic leaves after 3 days; For those who use artificial feed, spray the sur...

Embodiment 2

[0030] Example 2 Tea caterpillar virus production process

[0031] (1) Feeding of larvae

[0032] 1. One pair of adults (female, male) are raised in a glass can (glass container), and the adults are taken out after laying eggs. ,

[0033] 2. When the above eggs hatch into larvae, they should be fed with feed (tea or artificial feed), and feed should be added before the feed is finished. As the larvae continue to grow, the larvae in one bottle are divided into 3 or more bottles, and finally the number of larvae in each bottle is about 50. Feeding with artificial feed should be divided into several bottles, 10-15 heads per bottle.

[0034] (2) Virus reproduction (multiplication)

[0035] 3. When the above-mentioned larvae are reared at 24°C for 25 days, add poisonous leaves (the leaves are soaked in the 005 million polyhedron / ml virus solution and then taken out and dried). After 3 days, they will be reared with non-toxic leaves, artificially For feed feeding, use a throat sprayer a...

Embodiment 3

[0047] Example 3 Production process of Platypus virus

[0048] (1) Feeding of larvae

[0049] 1. One pair of adults (female, male) are raised in a glass can (glass container), and the adults are taken out after laying eggs.

[0050] 2. When the above eggs hatch into larvae, they should be fed with feed (tea or artificial feed), and feed should be added before the feed is finished. As the larvae continue to grow, the larvae in one bottle are divided into 3 bottles or more bottles, and finally the number of larvae in each bottle is about 50. Feeding with artificial feed should be divided into a few more bottles, each bottle of 10-15.

[0051] (2) Virus reproduction (multiplication)

[0052]3. When the above-mentioned larvae are reared at 24°C for 15 days, add poisonous leaves (the leaves are soaked in a 005 million polyhedron / ml virus solution and then taken out and dried). After 3 days, they will be reared with non-toxic leaves, artificially For feed raising, use a throat sprayer a...

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Abstract

A collecting method in insect viruses breed includes: 1) feeding three-year old larva by 500,000 polyhedrons / ml concentration for 3 days, 2) primary collecting larva after experimenting 7-8 days under 24deg.C environment,3) extracting virus and processing viral imagocide after reserving 20-30 days under 15deg.C homeothermal condition. It achieves high insect virus output, simple viral collecting process, electrical save.

Description

Technical field [0001] The present invention relates to an insect virus propagation technology, more specifically, the present invention relates to a high-yield and collection method in insect virus propagation. Background technique [0002] Tea looper, tea caterpillars, and spine moths are the main leaf-eating pests of tea trees. In order to reduce the residual amount of chemical pesticides in tea, the application of insect virus insecticides is an effective way of biological control of tea pests. But insect viruses can only reproduce in living organisms (cells), and cannot be mass-produced by fermentation in a culture medium like fungi and bacteria. So far, the method of insect virus production at home and abroad is to raise large numbers of larvae until they reach a certain age, so that the larvae feed on the virus infected body. The virus continues to reproduce in the larvae. When the body is full of virus, the larvae die and are collected daily after t...

Claims

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Application Information

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IPC IPC(8): A01K67/033A01N63/00C12N7/01
Inventor 殷坤山肖强唐美君郭华伟
Owner TEA RES INST CHINESE ACAD OF AGRI SCI
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