Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of gelatin/CMC insect virus microcapsules

A technology of insect virus and microcapsules, which is applied in botany equipment and methods, insecticides, animal repellants, etc., can solve the problems affecting the application of insect viruses and insufficient anti-ultraviolet performance, so as to improve the insecticidal rate and anti-ultraviolet Strong UV effect

Pending Publication Date: 2020-01-24
ZHONGKAI UNIV OF AGRI & ENG
View PDF8 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Insufficient UV resistance of existing insecticides for insect viruses hinders the application of insect viruses

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of gelatin/CMC insect virus microcapsules
  • Preparation method of gelatin/CMC insect virus microcapsules
  • Preparation method of gelatin/CMC insect virus microcapsules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A preparation method for gelatin / CMC insect virus microcapsules, comprising the following steps:

[0027] (1) Dissolve CMC in deionized water to obtain a CMC solution with a mass fraction of 1%; add 0.1 g of virus dry powder to 50 g of CMC solution, stir and mix evenly to form mixture A; prepare a mass fraction of 1 with the ready-to-use buffer solution % gelatin solution;

[0028] (2) Add gelatin solution to mixture A, wherein the mass ratio of gelatin to CMC is 9:1; stir until the solution is mixed evenly at a temperature of 35°C, and the rotating speed is 400r / min; the mass percentage is 10% added dropwise to the solution glacial acetic acid solution, slowly adjust the pH value of the solution until the pH value is 4.632 (at this time, draw 1ml of the solution and save it as sample 1), stop heating, cool the solution, add 2ml of 1mg / ml tea polyphenol aqueous solution dropwise, react for 30min, Leave to stand and centrifuge, remove the supernatant, and freeze-dry the...

Embodiment 2

[0031] A preparation method for gelatin / CMC insect virus microcapsules, comprising the following steps:

[0032] (1) Dissolve CMC in deionized water to obtain a CMC solution with a mass fraction of 1%; add 0.5 g of virus dry powder to 100 g of CMC solution, stir and mix evenly to form mixture A; prepare a mass fraction of 1 with the ready-to-use buffer solution % gelatin solution;

[0033] (2) Add gelatin solution to mixture A, wherein the mass ratio of gelatin to CMC is 2:1; stir at 30°C until the solution is evenly mixed, and the rotating speed is 600r / min; dropwise into the solution, the mass percentage is 10% glacial acetic acid solution, slowly adjust the pH value of the solution until the pH value is 4.632, stop heating, let the solution cool down, add 1ml of 0.5mg / ml formaldehyde solution dropwise, react for 30min, let stand and centrifuge, remove the supernatant, and freeze the precipitate Dried is gelatin / CMC virus microcapsule.

[0034] The virus dry powder is Spod...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of gelatin / CMC insect virus microcapsules. The method comprises the following steps: (1) dissolving CMC in deionized water to obtain a CMC solution; addingan insect virus dry powder into the CMC solution, and performing stirring and mixing uniformly to form a mixture A; and preparing a gelatin solution with a mass percentage of 1%-5% by using a ready-made buffer solution; and (2) adding the gelatin solution into the mixture A, wherein a mass ratio of gelatin to CMC is 1:2-12:1; performing stirring at a temperature of 25-35 DEG C with a revolution speed of 200-1000 r / min until the solution is mixed uniformly; and dropwise adding a glacial acetic acid solution with a mass percentage of 10% into the solution, slowly adjusting a pH value of the solution until spherical microcapsules appear in the solution observed under a microscope, stopping heating, performing cooling, dropwise adding a curing agent solution, carrying out a reaction for 30-60min, performing standing separation, removing a supernatant, and freeze drying a precipitate to obtain the gelatin / CMC virus microcapsules. The gelatin / CMC virus microcapsules can be applied to the Spodoptera litura polyhedrosis virus, is safe and environment-friendly, has strong anti-ultraviolet performance, can effectively protect the virus from being killed by ultraviolet rays, and improves aninsecticidal rate.

Description

technical field [0001] The invention relates to the technical field of slow-release microcapsule pesticides, in particular to a preparation method of gelatin / CMC insect virus microcapsules. Background technique [0002] The country is currently committed to developing a healthy China, advocating green development, and attaching great importance to ecological and environmental protection. Therefore, traditional pesticides with high toxicity, high pollution, difficult storage, poor physical and chemical stability, and low utilization rate can no longer meet the needs of people today, which makes people pay more attention to pesticide mitigation while researching and inventing new pesticide products. Research on release and controlled release. [0003] Microcapsule technology uses natural or synthetic polymer materials as the capsule wall, and wraps the active substance (capsule core) by chemical, physical or physicochemical methods to form a semi-permeable or sealed capsule m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01N63/40A01N25/28A01P7/04
CPCA01N25/28
Inventor 蒲侠朱丹丹周新华舒绪刚
Owner ZHONGKAI UNIV OF AGRI & ENG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com