59 results about "Cecropin" patented technology

The invention discloses a housefly cecropin-human lysozyme fusion protein, and a preparation method and application thereof. A sequence of the housefly cecropin-human lysozyme fusion protein of the invention is shown by SEQ ID No.1. The housefly cecropin-human lysozyme fusion protein comprises a mature peptide sequence of housefly cecropin, a polypeptide linker sequence and the mature peptide sequence of human lysozyme. The housefly cecropin-human lysozyme fusion protein is prepared by the following steps of: according to the gene sequences of the housefly cecropin and the human lysozyme, designing a synthetic primer with esherichia coli preferred codons; performing PCR amplification by using a splicing overlap extension method to obtain the gene sequence of the fusion protein so as to construct recombinant prokaryotic expression plasmids; transferring the recombinant prokaryotic expression plasmids into host cells to obtain engineering bacteria expressing the fusion protein; and performing fermentation culture, separation and purification to obtain the housefly cecropin-human lysozyme fusion protein of the invention. The fusion protein of the invention has the characteristics of the housefly cecropin and the human lysozyme per se, has relatively more remarkable antibacterial activity and antibiotic activity, can be used for preparing antibacterials or bacteriostatic medicaments, and has wide application prospect.

The present invention discloses Cecropins obtained from Aedes aegypti, a coding gene and applications thereof. According to the present invention, the protein of the present invention is the following (a) or (b) or (c): (a) the protein comprises amino acid residues at sites 20-62 from the N terminal in the sequence 1 in the sequence table, (b) the protein comprises an amino acid sequence represented by the sequence 1 in the sequence table, and (c) the protein is formed by carrying out substitution, and/or deletion and/or addition of one or a plurality of amino acids on the (a) or (b), has the antibacterial peptide function, and is derived from the (a) or (b); results of the present invention further prove that the dengue virus infection capability can be effectively reduced with the AaCECN protein; and the broad application prospects are provided.

The invention discloses a plutella xylostella cecropin gene, an encoded protein, a corresponding expression system and application. The plutella xylostella cecropin gene is double stranded linear deoxyribonucleic acid (DNA) with the length of 480bp, and a nucleotide sequence of the plutella xylostella cecropin gene is shown in SEQ ID NO:1. The protein encoded by the gene has 65 amino acids, and an amino acid sequence of the protein is shown in SEQ ID NO:2. Plutella xylostella cecropin is expressed in Escherichia coli through a gene recombination technology, and the plutella xylostella cecropin with an antibacterial function is obtained. The plutella xylostella cecropin with the antibacterial function has broad application value in medicines, food preservation or adding of feed preservatives.

The invention discloses natural anti-inflammatory polypeptide cecropin-TY1 extracted from salivary glands of Tabancus yao. The natural anti-inflammatory polypeptide cecropin-TY1 comprises 39 amino acids, the carbon tail end is amidated, the molecular weight is 3970.22 Dalton, the isoelectric point is 11.17, the amino acid sequence is SEQ ID: 1, and all the amino acids are L-type. The natural anti-inflammatory polypeptide cecropin-TY1 has the characteristics of small molecular weight, remarkable anti-inflammatory effect and low cytotoxicity, has wide application prospects in anti-inflammatory drugs, drugs for treating septicopyemia and endotoxin shock caused by bacterial infection, veterinary drugs, animal feed and cosmetics and is particularly applied to inhibiting lipopolysaccharide induced macrophages from generating nitric oxide, inhibiting lipopolysaccharide induced macrophages from generating inflammatory cell factors, inhibiting a lipopolysaccharide induced inflammation signal path as well as neutralizeing the endotoxin.

The invention provides a plant expression vector for controlling an artificially synthesized antimicrobial peptide gene by using a specific vascular promoter and a method for culturing anti-greensickness cotton by using the same. In the method, an artificially synthesized antimicrobial peptide gene spCEMA (Signal Peptide modified Cecropin A-Melittin) under the control of a specific vascular promoter AAP2 is integrated to a cotton genome to realize the specific expression of the gene spCEMA in a cotton vascular tissue, so that the disease resistance of cotton to greensickness is obviously improved. By inoculating different pathogenic bacteria of different greensickness types to genetically modified cotton obtained with the method in a homozygosis plant infected field, a disease index is less than 15, which is reduced by more than 85% in contrast with that of non-genetically modified cotton.

The invention discloses a process method for fusion expression of antimicrobial peptides (CAD and Buforin II) with two different bactericidal mechanisms and forming two antimicrobial peptide composite preparations by using hydroxylamine cutting. Hydroxylamine can be used to specifically cut an Asn-Gly peptide bond in protein, an Asn-Gly site is introduced in a connecting part of a fusion protein and an additional sequence (His tag), and the fusion protein is efficiently expressed by an escherichia coli expression system; and after affinity chromatography purification, a recombined CAD-Buforin II is obtained; and the recombined CAD-Buforin II is cut by hydroxylamine again, and is continuously separated and purified to obtain the purified CAD and Buforin II composite antimicrobial peptides. The fusion protein provided by the invention can be used as an animal feed additive part to replace the traditional antibiotic additive.

The invention discloses cerate special for blackwood furniture and a preparation method for the cerate. The cerate comprises the following components in parts by weight: 4 to 8 parts of beeswax, 2 to 5 parts of white oil, 0.3 to 1.6 parts of insect wax, 1.5 to 2.5 parts of sweet orange oil, 4 to 6 parts of seaweed polysaccharide and 5 to 7 parts of cecropin. According to the antibacterial marine organism component-containing cerate special for the blackwood furniture, the seaweed polysaccharide and the cecropin serve as biological sterilization and antibacterial components, so that the cerate is non-corrosive, and the blackwood furniture can be effectively prevented from mildew in a humid environment; the cerate has the advantages of protecting the glossiness of the blackwood furniture, preventing cracking, filling and repairing scratches, keeping the blackwood furniture like new, improving the glossiness of the blackwood furniture and prolonging the service life of the blackwood furniture after being used for a long time.

The invention belongs to the technical field of citrus quality improvement, and particularly relates to a plant expression vector for antimicrobial peptide induced expression and application thereof. The invention aims to provide a new option for improving citrus canker resistance. The technical scheme is an expression cassette for antimicrobial peptide induced expression. The invention is characterized in that the citrus canker pathogen is adopted to efficiently induce the expression of the expression promoter control cecropin antimicrobial peptide. The invention also relates to a plant expression vector containing the expression cassette and a host cell containing the plant expression vector. The invention also relates to a method for enhancing citrus canker resistance. The antimicrobial peptide gene mAATCB under the control of the canker high-efficiency promoter is integrated into the citrus genome, thereby implementing quick high-level expression of the antimicrobial peptide gene when the citrus is infected by the canker pathogen, and further enhancing the citrus canker resistance.

The invention provides a method for culturing anti-greensickness cotton by using an artificially synthesized antimicrobial peptide gene. In the method, the artificially synthesized antimicrobial peptide gene is integrated into upland cotton cultivated species to realize the constitutive expression of the antimicrobial peptide gene spCEMA (Signal Peptide modified Cecropin A-Melittin) in genetically modified cotton so as to improve the disease resistance of the cotton to greensickness. In the invention, a gene CEMA with good disease-resistance effect is fused, the toxic and harmful effects to plant cells by in-vivo expression of a protein CEMA can be eliminated, and growth and development of the plant are not influenced while the disease resistance of the plant is improved. In the invention, by means of the constitutive expression of the artificially synthesized antimicrobial peptide gene spCEMA, the verticillium lecanii can be controlled within a certain range or can be inhibited in further growth in the plant, so that the purpose of resisting the greensickness is achieved and novel anti-greensickness cotton resources are cultured. By applying the method, the genetically modified anti-greensickness cotton with the disease index smaller than 10 or so can be obtained.

The invention belongs to the field of bio-genetic engineering and provides a shuttle plasmid and a method for efficiently expressing cecropin and lysozyme genes. The invention constructs an escherichia coli-bacillus subtilis shuttle plasmid suitable for being expressed in prokaryotes (escherichia coli, bacillus subtilis and bacillus sphaericus). Cecropins expressed in the prokaryotes does not have a toxic action on hosts. The shuttle plasmid can successfully express the fusion protein of various cecropins such as human lysozyme, bovine lactoferrin, and the like. 6His is introduced into the expression plasmid constructed by the invention, which is convenient to purify, and the purified fusion protein identifies and correctly cut enzyme cutting sites among all fusion peptide segments through lysostaphin so as to release all functional peptides and enable the functional peptides to recover the activity; and moreover, enzyme separation and cutting are also saved, thus the method can be effectively, simply and conveniently used. The shuttle plasmid also has a certain common availability, and all suitable genes can be cloned and expressed.

The invention discloses an antimicrobial peptide cecropin A mutant and a coding gene, a preparation method and application thereof. The amino acid sequence of the mutant is as shown in SEQ ID No: 3. The nucleotide sequence of a gene for encoding the above mutant is as shown in SEQ ID No: 4. Molecular cloning is carried out according to the gene sequence of the antimicrobial peptide mutant PEW300 at the design site, and the gene was cloned into a recombinant expression vector; and through fusion expression with a self-aggregation short-peptide ELK16, high-efficiency expression of the mutant PEW300 is realized. Then, bacteriostasis performance test of 9 indicator bacteria is carried out on the mutant peptide PEW300, and the results show that bacteriostasis performance of the antimicrobial peptide PEW300 is greatly improved and the antimicrobial peptide PEW300 has strong pH and heat stability.

The invention discloses antimicrobial peptide effervescent tablets, which comprise the following components by weight percent: 5-20% of cecropin antimicrobial peptide, 21% of tartaric acid, 15-32% of sodium bicarbonate, 4% of polyvinylpyrrolidone, 25.5-40.5 of starch, and 0.5% of magnesium stearate. The invention also discloses a preparation method of the antimicrobial peptide effervescent tablets and application thereof in treating hysteritis in milk cattle. The antimicrobial peptide effervescent tablets disclosed by the invention use cecropin antimicrobial peptide as a raw material, and have the following advantages: (1) the raw materials are commonly used and easily available, so as to save cost; (2) the process route is simple, so as to be favor of production; (3) due to unique disintegration manner, large contact surface, sustained release and other characteristics, the effervescent tablets for treating hysteritis in milk cattle are suitable for uterus drug delivery, so as to effectively increase drug concentration, improve antimicrobial effect and improve clinical cure rate; and (4) the tablets are convenient to carry and simple in administration, and have rapid action, good stability and other advantages.

The invention provides an antibacterial temperature-controlled micro-nano fiber and a preparing method thereof, and belongs to the technical field of fiber materials. Sericin with the antibacterial property is grafted by tyrosinase and cecropin, and the antibacterial property of the sericin is further improved. Moreover, a modified sericin solution is mixed with N-isopropylacrylamide, N,N-methylene bisacrylamide, ammonium persulfate, N,N,N,N-tetramethylethylenediamine and acetic acid, and gel with temperature sensitivity is obtained through free radical polymerization. In addition, by adjusting the microfluid spinning flow rate to be 10-40 microliters per second and the spinning rate to be 10-90 rad/min, the specific surface area of the micro-nano fiber is increased, the fiber nanometer effect is improved, and the antibacterial performance of the micro-nano fiber is improved. It shows through data that the antibacterial temperature-controlled micro-nano fiber has the good antibacterialproperty and the antibacterial rate can reach 99.99% or above at most.

The present invention discloses diamondback moth cecropin 3, a preparation method and an application thereof, wherein the diamondback moth cecropin 3 gene has a nucleotide sequence represented by SEQIDNO:1-2 and has a corresponding amino acid sequence represented by SEQIDNO:3, and sequence analysis results show that the mature peptide of the diamondback moth cecropin 3 has an amino acid sequence represented by SEQIDNO:4. According to the present invention, the diamondback moth cecropin 3 gene or the mature peptide sequence thereof is subjected to prokaryotic expression to obtain the diamondback moth cecropin 3, and the obtained diamondback moth cecropin 3 provides strong inhibition effects for gram-negative bacteria and gram-positive bacteria, provides strong inhibition effects for pathogenic fungi, especially provides strong inhibition effects for a lot of pathogenic fungi of melons and fruits, and is expected to be a novel peptide antibiotic.

The invention relates to marine biotin degerming liquid for treating onychomycosis (leuconychia) and paronychia. The marine biotin degerming liquid is prepared by compounding beta-chitosan quaternize hyaluronic acid derivatives, alga polysaccharide derivatives, marine biological alpha-helical cecropin, marine biotin, cationic polymerization guanidine, glycerol and an alga agent. The marine biotin degerming liquid is mainly used for treating various types of onychomycosis (leuconychia) and paronychia, and can treat onychomycosis (leuconychia) and paronychia which are caused by various bacteria. Compared with the conventional treatment liquid, the marine biotin degerming liquid has the advantages of high sterilizing rate, no toxicity, no irritation, no corrosion, short cure time and the like. According to a formula, the marine biotin degerming liquid for treating the onychomycosis (leuconychia) and the paronychia comprises the following components in percentage by weight: 1.0 to 2.0 percent of beta-chitosan quaternize hyaluronic acid derivatives, 0.5 to 1.0 percent of alga polysaccharide derivatives, 6.0 to 8.0 percent of marine biological alpha-helical cecropin, 3.0 to 4.0 percent of cationic polymerization guanidine, 0.5 to 1.0 percent of marine biotin, 2.0 to 3.0 percent of glycerol, 1.0 to 4.0 percent of alga agent and 100 percent of deionized water.

The invention belongs to compound Flunixin meglumine injection and a preparation method thereof. The compound Flunixin meglumine injection comprises, by weight percentage, 8-10% of Flunixin meglumine, 1-5% of honeysuckle extract, 1-5% of baicalin, 3-10% of taurine, 1-10% of cecropin, 10-15% of polyoxyethylene 40 hydrogenated castor oil, 10-21% of propylene glycol, 1-20% of ethanol and 0.5-1% of tween 80. The compound Flunixin meglumine injection has the advantages that the compound Flunixin meglumine injection combines Chinese and western medicine, has antipyretic and analgesic effects and antibacterial and anti-inflammation effects, and is capable of increasing organism immunity and disease resistance and evidently changing clinical symptoms; the compound Flunixin meglumine injection is long in curative effect duration, the cost is lowered, stress reactions, caused by multiple injection, of animals are reduced, bacterium drug resistance cannot be generated easily, and the compound Flunixin meglumine injection is low in toxicity, reliable in curative effect and lasting in effect.

The invention discloses a marine organism antiseptic solution special for underwear and a preparation method thereof, the marine organism antiseptic solution comprises the following components: by weight, 1.0-2.0% of carboxymethyl chitosan; 2.0-4.0% of seaweed polysaccharide; 6.0-8.0% of cecropin; 1.5-3.5% of N-acetylmuramoylhydrolase; 6.0-9.0% of palmitic cationic ester quaternary ammonium; and the balance of purified water. The average killing rates of the marine organism antiseptic solution special for the underwear on staphylococcus aureus, escherichia coli and candida albicans are 100%; and the marine organism antiseptic solution has the advantages of to good sterilization effect, and no corrosiveness to clothing and stimulation on the skin. The marine organism antiseptic solution contains the palmitic cationic ester quaternary ammonium comprising aliphatic chains with ester bonds, has a good biodegradable performance, is a substitute of traditional softening tablet, softening oil and softening ointment, is a new environmentally friendly clothing softening and smoothing component, has good softness, antistatic property and a certain sterilization function, and is excellent in water solubility, and the underwear may not be yellowing after use of the marine organism antiseptic solution.

The invention discloses liver targeting trans-membrane antiviral fusion polypeptide as well as a coding gene and an application thereof. The fusion polypeptide is polypeptide consisting of an amino acid residue sequence shown in SEQ IDNO: 1. The invention further discloses a coding gene of liver targeting cell-penetrating peptide-cecropin fusion peptide, a nucleotide sequence of the coding gene is SEQ IDNO: 2. The fusion polypeptide has the following advantages: 1) the positioning is accurate, and the combination is highly-efficient; 2) competitive blocking and intracellular targeting killing are performed; and 3) the toxic and side effects are small. The liver targeting trans-membrane antiviral fusion polypeptide provided by the invention has a larger practical significance and a wide application prospect in the preparation field of anti-HBV (Hepatitis B Virus) drugs.

The invention provides a peptide comprising: a core amino acid sequence, which is identical or similar to the amino acid sequence of a member of the Cecropin family. The invention further provides a nucleic acid sequence encoding the peptide and a vector comprising said nucleic acid. The invention further provides a pharmaceutical composition comprising said peptide or said nucleic acid. The invention further provides methods of treating an infection, overcoming inherent or acquired resistance of a microorganism to an antibiotic agent or disinfecting a wound, the methods comprises administering the peptide to a subject in need thereof.

The invention discloses a compound nursing agent for perianal eczema and a preparation method thereof. The nursing agent is prepared from, by weight, 12-36 parts of traditional Chinese medicine extracts, 11-25 parts of natural borneol, 3-7 parts of glycerol, 2-4 parts of cecropin, 0.2-1.6 parts of levamisole, 3-5 parts of ursolic acid, 0.6-1.0 part of alcohol alkoxylate, 0.5-1.0 part of an emulsifying agent, 0.4-0.5 part of penetration enhancer and 0.1-0.22 part of liquid stabilizer. The traditional Chinese medicine extracts comprise 1-3 parts of Ixeris chinensis extracts, 1-3 parts of caddis worm extracts, 1-3 parts of basil extracts, 1-3 parts of extracts of roots of fernleaf dichocarpum, 1-3 parts of ficus hirta extracts, 1-3 parts of pistacia weinmannifolia extracts, 1-3 parts of herba stellariae neglectae extracts, 1-3 parts of mosla dianthera extracts, 1-3 parts of phyla nodiflora extracts, 1-3 parts of cypress leaf extracts, 1-3 parts of platycarya strobilacea fruit extracts and 1-3 parts of sophora japonical extracts. Traditional Chinese medicine is combined with Western medicine, the compound nursing agent has the functions of being cool and relieving itch, dispelling wind and removing eczema, activating blood and eliminating dampness, closing sores and promoting tissue regeneration, diminishing inflammation and being anti-microbial, and being anti-infectious, the curative effect on perianal eczema is remarkable, perianal eczema is thoroughly cured, and perianal eczema is not prone to relapse after being cured.