Plant expression vector for controlling artificially synthesized antimicrobial peptide gene by using specific vascular promoter and method for culturing anti-greensickness cotton by using same
A technology of plant expression vector and specific promoter, applied in the field of plant genetic engineering, can solve the problems of reducing fiber quality, plant morbidity, death, etc., and achieves the effect of simple and easy method, remarkable effect and huge economic benefit.
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[0051] Below in conjunction with accompanying drawing, the present invention will be described in further detail, but the following description does not limit the present invention, any deformation and change of the present invention, as long as they do not depart from the spirit of the present invention, all should belong to the definition of the appended claims of the present invention scope.
[0052] The pharmaceutical reagents in the implementation examples of the present invention are domestic conventional chemical reagents that are not specified, and the materials and methods that are not specified are referred to "Molecular Cloning Experiment Guide" (Sambrook and Russell, 2001).
[0053] 1. Extraction of DNA
[0054] 1.1 DNA extraction buffer:
[0055] (1) Plant DNA extraction buffer:
[0056] CTAB extract: 100 mmol / L Tris-HCl (pH8.0), 20 mmol / L EDTA (pH8.0), 1.5 mol / L NaCl, 2% CTAB (w / v), 4% PVP40 (w / v ) and 2% mercaptoethanol (v / v), PVP and mercaptoethanol were add...
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