A biocontrol strain and its application in preventing and controlling the soft rot of Fritillaria fritillary and promoting the growth of Fritillaria
A technology of biocontrol strains and fritillary fritillaria, applied in application, pest control, plant growth regulator, etc., can solve the problem of lack of biocontrol bacteria for fritillary fritillaria soft rot, and achieve good effect of disease prevention and production increase , reduce planting costs, and control infestation
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Embodiment 1
[0032] Example 1-Isolation, screening and identification of biocontrol strain Dm97
[0033] The present embodiment is the screening and identification process of the biocontrol strain Dm97 (Bacillus sp.), which specifically includes the steps:
[0034] (1) Screening of biocontrol strain Dm97
[0035] Source of strain: Dm97 was isolated from the root soil of Fritillaria fritillary, Nantong;
[0036] Strain isolation method: plate dilution method: sterilize 6 test tubes containing 9 mL of water, and press 10 to 10 6 Numbered in the order of the number, pipette to absorb 1 mL of cultured bacterial solution, pour it into a 10-fold diluted test tube, gently press the rubber on the pipette with your finger, blow and suck three times, and mix the bacterial solution and water well, from 10 times Pipette 1mL of diluent into the diluted test tube and inject 10 2 Repeat the mixing operation of the second step in the test tube with the doubling dilution. And so on until the dilution o...
Embodiment 2
[0043] Example 2-Cultivation of biocontrol strain Dm97 and preparation of its inoculum
[0044] This embodiment is a preferred method for culturing the biocontrol strain Dm97 obtained by screening in Example 1 and a method for preparing a bacterial agent thereof. The specific steps include:
[0045] (1) Cultivation of biocontrol strain Dm97
[0046] The biocontrol strain Dm97 was inoculated into LB medium (LB formula: sodium chloride 10g / L, tryptone 10g / L, yeast extract 5g / L, pH adjusted to 7.2). Scribe on the plate and cultivate in a biochemical incubator at 28°C for 14-16h. After a single colony grows, pick a single colony and put it into a test tube containing 5 mL of LB culture solution, and place it on a shaker at 28°C and 200 r / min. Cultivated for 25h in medium to make seed solution; inoculate the seed solution in a conical flask containing 500mL of LB culture solution with 1% of the inoculum, and place it in a shaker at 28°C and 200r / min for 48h. The prepared culture ...
Embodiment 3
[0049] Example 3-Application of microbial inoculum prepared by biocontrol strain Dm97
[0050] In this Example 3, the microbial inoculum prepared by the biocontrol strain Dm97 prepared in Example 2 was diluted with water to 5×10 7 Shake well after CFU / mL, and verify the disease prevention and control of Fritillaria fritillary. The specific steps include:
[0051] (1) Field disease prevention test (Zhejiang fritillary soft rot)
[0052] The field experiment was carried out in a professional cooperative of traditional Chinese medicine planting in Dongcheng, Hanyang Village, Haian County, Nantong City, Jiangsu Province, and the test plant was Fritillaria thunbergii Miq.
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