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Pesticide for inhibiting acquisition and transmission of geminivirus by Bemisia tabaci and method for controlling transmission of geminivirus

A technology of geminivirus and whitefly, which is applied in the field of controlling the spread of geminivirus, spreading drugs of geminivirus, and inhibiting the acquisition of whitefly, so as to ensure the healthy development and hinder the spread of spread

Active Publication Date: 2020-01-03
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, targeting the endocytosis receptor protein of vector insects that help viruses infect midgut epithelial cells can provide an effective reference for inhibiting virus transport and transmission, and there are no related reports so far

Method used

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  • Pesticide for inhibiting acquisition and transmission of geminivirus by Bemisia tabaci and method for controlling transmission of geminivirus
  • Pesticide for inhibiting acquisition and transmission of geminivirus by Bemisia tabaci and method for controlling transmission of geminivirus
  • Pesticide for inhibiting acquisition and transmission of geminivirus by Bemisia tabaci and method for controlling transmission of geminivirus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The effect of feeding anti-CUBN antibody on the ability of Bemisia tabaci to obtain and transmit the virus.

[0030] 1. Feeding anti-CUBN antibody affects the efficiency of Bemisia tabaci obtaining geminivirus in the early stage

[0031] (1) Dissolve the anti-CUBN antibody (Cat.No. ab191073; Abcam; 0.712mg / ml) in 15% sucrose solution with a dilution ratio of 1:50. At the same time, the rabbit preimmune serum (Cat.No.A7016; Beyotime; 1mg / ml) ml) use ddH 2 O was diluted to 0.712mg / ml and dissolved in 15% sucrose water at a ratio of 1:50.

[0032] (2) Take a cylindrical double-pass glass tube with an inner diameter of 2.4cm, an outer diameter of 2.7cm, and a height of 10cm. Pull the Parafilm membrane to one end of the double-pass glass tube, and drop 250μl of sucrose solution containing anti-CUBN antibody. At the same time, use the sucrose solution dissolved in rabbit preimmune serum as a negative control, and then apply a second layer of Paraflim so that the solution evenly cov...

Embodiment 2

[0064] The effect of dsAMN injection on the ability of Bemisia tabaci to obtain and transmit toxin.

[0065] 1. Injection of dsAMN affects the efficiency of Bemisia tabaci obtaining geminivirus in the early stage

[0066] (1) Design specific primers for AMN gene, and use cDNA as template to amplify double-stranded DNA (dsAMN for short), the sequence information is shown in SEQ ID NO. 1 (5'→3');

[0067] (2) Use the amplified double-stranded DNA as a template to synthesize double-stranded RNA by in vitro transcription;

[0068] (3) Purify the double-stranded RNA by phenol-chloroform extraction method;

[0069] (4) Use capillary tube to introduce double-stranded RNA into MEAM1 Bemisia tabaci by microinjection, with dsGFP (sequence shown in SEQ ID NO. 2) as a negative control;

[0070] (5) After 48 hours, transfer the treatment group and the control group Bemisia tabaci to tomatoes infected with tomato yellow leaf curl virus (TYLCV) for 1 hour and 2 hours respectively;

[0071] (6) Collect t...

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Abstract

The invention discloses application of CUBN protein and AMN protein or coding genes of the CUBN protein and AMN protein in a Bemisia tabaci CUBN-AMN complex as targets in inhibition of acquisition andtransmission of geminivirus by Bemisia tabaci, and also discloses pesticide for inhibiting acquisition and transmission of geminivirus by Bemisia tabaci and a method for controlling transmission of the geminivirus. The membrane receptor complex CUBN-AMN at the top of intestinal epithelial cells of Bemisia tabaci is targeted, the combination of multiple geminiviruses with Bemisia tabaci is taken as an example, and entry of the geminivirus into the intestinal epithelial cells of Bemisia tabaci is disturbed, so that the ability of Bemisia tabaci for acquisition and transmission of the geminivirus is reduced; and when the method is compared with existing methods and technologies, transmission of the geminivirus in the field is hindered from the source of transmission, and through combinationof a traditional chemical control method and adoption of multiple strategies, healthy development of agricultural production is ensured finally.

Description

Technical field [0001] The present invention relates to the technical field of plant virus prevention and control, in particular to drugs for inhibiting the acquisition and transmission of geminivirus by whitefly and a method for controlling the transmission of geminivirus. Background technique [0002] Gemini virus is an important type of single-stranded circular DNA plant virus worldwide, in the form of twin particles. This type of virus can infect a variety of cash crops including tomato, tobacco, and cotton, causing plants to produce symptoms such as dwarfing, yellowing, and curling, and ultimately cause crop yields or even no crops, and seriously threaten the sustainable development of global agriculture. The virus is transmitted by the major agricultural pest Bemisia tabaci in a long-lasting cycle. At this stage, farmers mainly rely on chemical control methods to control the occurrence of Bemisia tabaci, thereby indirectly reducing the harm of Gemini virus. However, the t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N57/16A01N61/00A01P1/00A01P7/04
CPCA01N57/16A01N61/00
Inventor 王晓伟赵静雷藤张新家刘树生
Owner ZHEJIANG UNIV
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