Diamondback moth cecropin 3, preparation method and application thereof

A technology of cecropin and diamondback moth, applied in the field of genetic engineering, can solve the problems of inactivity, toxicity and very sensitive protease of antimicrobial peptides, and achieve the effect of strong inhibitory effect
CN103484467AActive Publication Date: 2014-01-01SOUTH CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN ยท China
Current Assignee / Owner
SOUTH CHINA AGRI UNIV
Publication Date
2014-01-01

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Abstract

The present invention discloses diamondback moth cecropin 3, a preparation method and an application thereof, wherein the diamondback moth cecropin 3 gene has a nucleotide sequence represented by SEQIDNO:1-2 and has a corresponding amino acid sequence represented by SEQIDNO:3, and sequence analysis results show that the mature peptide of the diamondback moth cecropin 3 has an amino acid sequence represented by SEQIDNO:4. According to the present invention, the diamondback moth cecropin 3 gene or the mature peptide sequence thereof is subjected to prokaryotic expression to obtain the diamondback moth cecropin 3, and the obtained diamondback moth cecropin 3 provides strong inhibition effects for gram-negative bacteria and gram-positive bacteria, provides strong inhibition effects for pathogenic fungi, especially provides strong inhibition effects for a lot of pathogenic fungi of melons and fruits, and is expected to be a novel peptide antibiotic.
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Claims

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