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Bacteria removing solution and preparation method thereof

A sterilizing liquid and bactericidal peptide technology, which is applied in the direction of pharmaceutical formulations, local antibacterial agents, and medical preparations containing active ingredients, etc., can solve the problems of skin irritation, low bactericidal efficiency, and difficulty in rinsing, and reach the use concentration Low, improve sterilization effect, good sterilization effect

Inactive Publication Date: 2013-04-17
QINGDAO HIFUN MARINE BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many kinds of sterilizing liquids, antibacterial liquids, and disinfectant liquids with different bactericidal properties. Most of the active ingredients are chlorine, iodine, aldehydes, alcohols, acids, etc., which have low bactericidal efficiency, poor stability, toxicity, and corrosiveness. And pungent smell, irritating damage to the skin, and difficult to rinse off

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A kind of sterilizing liquid, comprises each component of following percentage by weight:

[0019] Carboxymethyl chitosan 1.0~2.0%;

[0020] Seaweed polysaccharide 2.0~4.0%;

[0021] Cecropin 6.0~8.0%;

[0022] N-acetyl murein hydrolase 1.5~3.5%;

[0023] Water 82.5~89.5%.

[0024] The sterilizing solution of this embodiment uses carboxymethyl chitosan, seaweed polysaccharide, and cecropin as the main disinfection components, which can destroy the cell membrane of harmful bacteria, cause irreversible denaturation of proteins and nucleic acids in the bacteria, and cause cell dysfunction, resulting in Bacteria die. It can also inhibit the synthesis of bacterial cell walls, and is especially sensitive to most Gram-positive bacteria such as Staphylococcus aureus and β-hemolytic streptococci, and has a strong killing effect on fungi. N-acetyl murein Glycan hydrolase acts as a synergistic ingredient in this formula to enhance the antibacterial effect. For Staphylococcus...

Embodiment 2

[0036] This embodiment provides a kind of preparation method that is used in the sterilizing liquid in embodiment 1, comprises the steps:

[0037] Step S10: adding carboxymethyl chitosan and seaweed polysaccharide in proportion to water at 30-35°C, stirring and mixing to obtain a mixed solution A;

[0038] Step S20: Add a proportioned amount of cecropin into the mixed solution A, stir and mix evenly to obtain the mixed solution B;

[0039] Step S30: Adding a proportioned amount of N-acetylmuramohydrolase into the mixed solution B, stirring and mixing evenly to obtain the sterilizing solution.

[0040] Preferably, in order to make the carboxymethyl chitosan and seaweed polysaccharide mix more uniformly in the water, in the step S10, the temperature of the water is 35°C.

[0041] Preferably, in order to make the carboxymethyl chitosan and seaweed polysaccharide mixed more uniformly in the water, in the step S10, the stirring and mixing time is 10-20 minutes.

[0042] Preferabl...

Embodiment 3

[0046] The present embodiment provides a kind of marine organism sterilization liquid, comprises each component of following percentage by weight:

[0047] Carboxymethyl Chitosan 1.0%;

[0048] Seaweed polysaccharide 2.0%;

[0049] Cecropin 6.0%;

[0050] N-acetylmuramohydrolase 1.5%;

[0051] Deionized water 89.5%.

[0052] The preparation method of the sterilizing liquid in the present embodiment comprises the steps:

[0053] Step S301: adding carboxymethyl chitosan and seaweed polysaccharide in proportion to water at 30°C, stirring and mixing for 10 minutes to obtain a mixed solution A;

[0054] Step S302: Add a proportioned amount of cecropin to the mixed solution A, stir and mix for 3 minutes to obtain the mixed solution B;

[0055] Step S303: Adding a proportioned amount of N-acetylmuramohydrolase to the mixed solution B, stirring and mixing for 7 minutes to obtain the sterilizing solution.

[0056] After testing: the degerming solution in the present embodiment ac...

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PUM

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Abstract

The invention discloses a bacteria removing solution and a preparation method thereof. The bacteria removing solution comprises the following components in percent by weight: 1.0-2.0% of carboxymethyl chitosan, 2.0- 4.0% of algal polysaccharides, 6.0-8.0% of cecropin, 1.5-3.5% of N-acetyl murein glycan hydrolytic enzyme, and 82.5-89.5% of water. When the bacteria removing solution is applied to staphylococcus aureus, escherichia coli and candida albicans for 2 min, the average kill ratio is higher than 99.9%, and while the bacteria removing solution is applied to staphylococcus aureus, escherichia coli and candida albicans for 5 min, the average kill ratio is 100%. The bacteria removing solution has the advantages of low use concentration and good sterilizing effect.

Description

[0001] The present invention claims the priority of the Chinese invention patent with the application number 201210545613.4, the application date is 2012.12.14, and the invention name is "sterilizing liquid and its preparation method". technical field [0002] The invention relates to a bactericidal substance, in particular to a bactericidal liquid and a preparation method thereof. Background technique [0003] The outbreak and mutation of new epidemic diseases have made people pay more and more attention to sterilization prevention, and bactericidal liquid has penetrated into all aspects of life. There are many kinds of sterilizing liquids, antibacterial liquids, and disinfectant liquids with different bactericidal properties. Most of the active ingredients are chlorine, iodine, aldehydes, alcohols, acids, etc., which have low bactericidal efficiency, poor stability, toxicity, and corrosiveness. And pungent smell, irritating damage to the skin, and more difficult to rinse o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/46A61P31/02A61K31/722A61K31/734A61K31/726
CPCY02A50/30
Inventor 李艳妮
Owner QINGDAO HIFUN MARINE BIOLOGICAL TECH
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