Composition for lowering blood sugar, blood lipids and blood pressure and preparation method thereof

Abstract

The present invention relates to a composition for lowering blood sugar, blood lipid and blood pressure and a preparation method thereof, belonging to the field of biotechnology, and the composition comprises a Cyclocarya paliurus flower extract, a chicken heart extract, a mandarin fish head extract and a Gracilaria extract; the Cyclocarya paliurus flower extract is obtained by enzymolysis of Cyclocarya paliurus flower by hemicellulase and bromelain, and then by ficin; the chicken heart extract is obtained by graded enzymolysis of chicken heart by pepsin and subtilisin; the mandarin fish head extract is obtained by fermenting mandarin fish head by Aspergillus niger, and then by trypsin; the Gracilaria extract is obtained by graded enzymolysis of Gracilaria by acid protease and trypsin. The composition has good effects of lowering blood sugar, blood lipid and blood pressure, can be used for preventing and treating cardiovascular and cerebrovascular diseases, softening blood vessels, improving microcirculation, reducing three high symptoms, and has no toxic side effects.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology, and specifically relates to a composition for lowering blood sugar, blood lipids and blood pressure and a preparation method thereof. Background Art

[0002] With the changes in people's lifestyles and eating habits, the acceleration of the aging process and the influence of genetic factors, cardiovascular and cerebrovascular diseases and the three high symptoms have become the main diseases that seriously threaten human health in today's society, and the incidence rate is showing an upward trend.

[0003] The pathological basis of cardiovascular and cerebrovascular diseases, such as coronary heart disease, myocardial infarction, and stroke, is often closely related to atherosclerosis. Atherosclerosis is caused by the combined effects of multiple factors, including vascular endothelial damage, lipid deposition, and inflammatory response. Long-term high blood pressure increases the pressure on the blood vessel wall, damages endothelial cells, and promotes lipids to enter the blood vessel wall and deposit. High blood sugar can damage the vascular endothelium in various ways and promote the development of atherosclerosis. Hyperlipidemia, especially hypercholesterolemia, can cause cholesterol to deposit in the blood vessel wall and form plaques.

[0004] The three high symptoms are not only important risk factors for cardiovascular and cerebrovascular diseases, but they themselves can also cause damage to various organs and systems in the body. Hypertension increases the burden on organs such as the heart and kidneys, leading to complications such as ventricular hypertrophy and renal insufficiency. Long-term high blood sugar levels can cause serious complications such as diabetic retinopathy, diabetic nephropathy, and diabetic foot. High blood lipids increase the risk of diseases such as pancreatitis and fatty liver.

[0005] At present, in terms of drug prevention and treatment, there are many types of drugs for cardiovascular and cerebrovascular diseases and three high symptoms. Antihypertensive drugs such as angiotensin converting enzyme inhibitors, angiotensin II receptor antagonists, calcium channel blockers, diuretics and beta-blockers lower blood pressure through different mechanisms of action and reduce the risk of cardiovascular events. Hypoglycemic drugs include insulin, sulfonylureas, biguanides, α-glucosidase inhibitors, thiazolidinediones and new glucagon-like peptide-1 receptor agonists, which help control blood sugar levels and prevent diabetes complications. Lipid-lowering drugs mainly include statins, fibrates and cholesterol absorption inhibitors, which can lower blood lipids, stabilize plaques and reduce the occurrence of cardiovascular and cerebrovascular diseases. In addition, antiplatelet drugs such as aspirin and clopidogrel can inhibit platelet aggregation and prevent thrombosis, playing an important role in the prevention and treatment of coronary heart disease and stroke. Anticoagulants such as warfarin and new oral anticoagulants are used to prevent thrombosis in diseases such as atrial fibrillation.

[0006] However, although existing drugs have achieved certain results in preventing and treating cardiovascular and cerebrovascular diseases and the three high symptoms, there are still some problems. For example, some patients have poor tolerance to drugs and may experience adverse reactions such as hypotension, hypoglycemia, liver and kidney damage, etc. At the same time, long-term use of drugs will increase the burden on the liver and kidneys, produce adverse side effects, organ failure, etc.

[0007] Therefore, it is necessary to continuously develop safer, more effective and convenient drugs and treatments to better prevent and treat cardiovascular and cerebrovascular diseases and the three high symptoms, and improve people's health level and quality of life. Summary of the invention

[0008] Existing therapeutic drugs for cardiovascular and cerebrovascular diseases and the three high symptoms generally have side effects, and long-term medication will increase the burden on the liver and kidneys; some patients have poor tolerance to drugs and may experience adverse reactions, such as hypotension, hypoglycemia, liver and kidney function damage and other problems. The present invention provides a composition for lowering blood sugar, blood lipids, and blood pressure and a preparation method thereof. The composition contains Cyclocarya paliurus flower extract, chicken heart extract, mandarin fish head extract, and Gracilaria extract. The composition is compounded in a certain proportion and has good effects of lowering blood sugar, blood lipids, and blood pressure. It can be used to prevent and treat cardiovascular and cerebrovascular diseases, soften blood vessels, improve microcirculation, and reduce the three high symptoms without any toxic side effects. The specific technical scheme is as follows:

[0009] A composition for lowering blood sugar, blood lipids and blood pressure, the composition is made of the following raw materials in parts by weight: 3 to 6 parts of Cyclocarya paliurus flower extract, 0.5 to 1.5 parts of chicken heart extract, 2 to 5 parts of Siniperca chuatsi head extract, and 0.5 to 1.5 parts of Gracilaria extract;

[0010] The Cyclocarya paliurus flower extract is an extract with a value below 3 kDa obtained by hydrolyzing the Cyclocarya paliurus flower with hemicellulase and bromelain and then with ficin; the chicken heart extract is an extract with a value below 5 kDa obtained by graded hydrolysis of chicken heart with pepsin and subtilisin; the mandarin fish head extract is an extract with a value below 3 kDa obtained by fermenting mandarin fish head with Aspergillus niger and then with trypsin; the Gracilaria extract is an extract with a value below 5 kDa obtained by graded hydrolysis of Gracilaria with acid protease and trypsin.

[0011] In the above technical scheme, the preparation method of the Cyclocarya paliurus flower extract is: crushing fresh Cyclocarya paliurus flowers, adding water 8 to 10 times the mass of the Cyclocarya paliurus flowers, adding 0.5% to 1.5% of hemicellulase by mass of the Cyclocarya paliurus flowers and 0.5% to 1.5% of bromelain by mass of the Cyclocarya paliurus flowers, enzymolysis at 50°C to 60°C for 1.5h to 2h, inactivating the enzyme at high temperature, cooling to room temperature, adding 1% to 2% of fig protease by mass of the Cyclocarya paliurus flowers, enzymolysis at 60°C to 70°C for 1h to 1.5h, inactivating the enzyme at high temperature, cooling to room temperature, filtering with a 3kDa ultrafiltration membrane to obtain a filtrate below 3kDa, spray drying, and obtaining a Cyclocarya paliurus flower extract.

[0012] In the preparation method of the Cyclocarya paliurus flower extract, the enzymatic activity of the hemicellulase is 100,000 U / g; the enzymatic activity of the bromelain is 100,000 U / g; the enzymatic activity of the ficin is 100,000 U / g;

[0013] The temperature of the high temperature enzyme inactivation is 70° C. to 80° C., and the time is 15 min to 25 min.

[0014] In the above technical scheme, the preparation method of the chicken heart extract is: crush the fresh chicken heart, wash it with ethanol aqueous solution for 3 to 5 times, and finally filter it to obtain a solid; then add 8 to 10 times the mass of water to the solid, adjust the pH to 1.5 to 2 with hydrochloric acid, add 1.5% to 2% of the mass of pepsin, hydrolyze it at 36°C to 40°C for 2h to 3h, inactivate the enzyme at high temperature, cool it to room temperature, adjust the pH to 7.8 to 8.5, add 0.8% to 1.5% of the mass of subtilisin, hydrolyze it at 40°C to 50°C for 1h to 1.5h, inactivate the enzyme at high temperature, cool it to room temperature, filter it with a 5kDa ultrafiltration membrane to obtain a filtrate below 5kDa, spray dry it, and obtain a chicken heart extract.

[0015] In the preparation method of the chicken heart extract, the volume concentration of the ethanol aqueous solution is 20% to 30%; the enzyme activity of the pepsin is 10,000 U / g; the enzyme activity of the subtilisin is 30,000 U / g; the temperature of the high-temperature enzyme inactivation is 80°C to 90°C, and the time is 15min to 25min.

[0016] In the above technical scheme, the preparation method of the mandarin fish head extract is: crush the mandarin fish head, wash it with ethanol aqueous solution for 3 to 5 times, and finally filter it to obtain a solid; then add 8 to 10 times the mass of water to the solid, adjust the pH to 4 to 5.5, add 1% to 2% of the mass of Aspergillus niger, ferment at 30°C to 37°C for 8d to 10d, sterilize at high temperature, cool to room temperature, adjust the pH to 7.8 to 8.5, add 0.5% to 1% of the mass of trypsin, hydrolyze at 35°C to 38°C for 2h to 3h, inactivate the enzyme at high temperature, cool to room temperature, filter it with a 3kDa ultrafiltration membrane to obtain a filtrate below 3kDa, spray dry it, and obtain the mandarin fish head extract.

[0017] In the preparation method of the above-mentioned mandarin fish head extract, the mandarin fish head does not contain fish gills; the volume concentration of the ethanol aqueous solution is 20% to 30%; the bacterial activity of the Aspergillus niger is 5 billion CFU / g; the enzyme activity of the trypsin is 100,000 U / g; the temperature of the high-temperature sterilization is 121°C to 125°C, and the time is 15min to 20min; the temperature of the high-temperature enzyme inactivation is 70°C to 80°C, and the time is 15min to 25min.

[0018] In the above technical scheme, the preparation method of the Gracilaria extract is: take Gracilaria and crush it, add 10 to 12 times the mass of sodium hydroxide aqueous solution of Gracilaria, mix at 60°C to 70°C for 1h to 3h, filter to obtain a solid; add 8 to 10 times the mass of water of the solid to the solid, adjust the pH to 2.5 to 3.0, add 0.5% to 1.5% of the solid mass of acid protease, enzymatically hydrolyze at 40°C to 60°C for 1h to 1.5h, inactivate the enzyme at high temperature, cool to room temperature, adjust the pH to 7.8 to 8.5, add 0.5% to 1% of the solid mass of trypsin, enzymatically hydrolyze at 35°C to 40°C for 1h to 1.5h, inactivate the enzyme at high temperature, cool to room temperature, filter with a 5kDa ultrafiltration membrane to obtain a filtrate below 5kDa, spray dry, and obtain the Gracilaria extract.

[0019] The preparation method of the above-mentioned Gracilaria extract is characterized in that the volume concentration of the sodium hydroxide aqueous solution is 8% to 12%, the enzyme activity of the acidic protease is 100,000 U / g; the enzyme activity of the trypsin is 100,000 U / g; the temperature of the high-temperature enzyme inactivation is 70°C to 80°C, and the time is 15min to 25min.

[0020] The preparation method of the above-mentioned composition for lowering blood sugar, blood lipids and blood pressure comprises the following steps:

[0021] S1: uniformly mixing the Cyclocarya paliurus flower extract, the Gastrodia elata extract, the Siniperca chuatsi extract and the Gracilaria truncatula extract according to the mass ratio to obtain a composition;

[0022] S2: Mixing the composition with pharmaceutically acceptable excipients to prepare tablets or capsules.

[0023] The present invention provides a composition for lowering blood sugar, blood lipids and blood pressure and a preparation method thereof, which has the following beneficial effects compared with the prior art:

[0024] 1. The blood sugar, blood lipid and blood pressure lowering composition of the present invention contains Cyclocarya paliurus flower extract, chicken heart extract, mandarin fish head extract and Gracilaria extract, which are compounded and used in a certain proportion, and have good blood sugar, blood lipid and blood pressure lowering effects, can be used to prevent and treat cardiovascular and cerebrovascular diseases, soften blood vessels, improve microcirculation, and reduce the three high symptoms without any toxic side effects.

[0025] 2. The Cyclocarya paliurus flower extract of the present invention is an extract of less than 3 kDa obtained by hydrolyzing the Cyclocarya paliurus flower with hemicellulase and bromelain and then with ficin; the extract contains various small molecule peptides and water-soluble substances that are specially hydrolyzed, which are easy to absorb, can promote the relaxation of vascular smooth muscle, increase the inner diameter of blood vessels, and thus lower blood pressure; regulate the renin-angiotensin-aldosterone system, reduce the production of vasoconstrictor substances, and lower blood pressure. It is helpful to repair and regenerate damaged pancreatic islet cells, improve insulin secretion function, make cells more sensitive to insulin, improve glucose uptake and utilization efficiency, and thus help lower blood sugar. It inhibits cholesterol synthesis, reduces the production of cholesterol in the body, promotes lipid metabolism, and lowers blood lipid levels.

[0026] 3. Chicken hearts are rich in protein and various nutrients. The chicken heart extract of the present invention is an extract below 5kDa obtained by graded enzymatic hydrolysis of chicken hearts with pepsin and subtilisin; the extract contains various specially hydrolyzed small molecule chicken heart peptides and water-soluble substances, which are easy to absorb. After absorption, the components can regulate vascular microcirculation, dilate blood vessels, and lower blood pressure; improve insulin secretion and sensitivity, increase cellular uptake and utilization of glucose, and thus improve blood sugar content; inhibit the synthesis and absorption of cholesterol, and promote the metabolism and excretion of cholesterol.

[0027] 4. Mandarin fish head is rich in protein, minerals and vitamins, and has a good nourishing effect. The mandarin fish head extract of the present invention is an extract below 3kDa obtained by fermenting mandarin fish head with Aspergillus niger and then enzymolysis with trypsin; it contains small molecule mandarin fish head hydrolyzed peptides and fermented products, which can regulate vascular microcirculation, dilate blood vessels, and lower blood pressure; affect the channels of calcium ions, potassium ions, etc., regulate the contraction and relaxation of vascular smooth muscles, and then affect blood pressure; stimulate pancreatic beta cells to release insulin, increase the content of insulin in the body, enhance the response of cells to insulin, promote the uptake and utilization of glucose, and improve blood sugar content; accelerate the decomposition and metabolism of fat, and reduce the accumulation of blood lipids.

[0028] 5. The Gracilaria extract of the present invention is an extract of less than 5 kDa obtained by graded enzymatic hydrolysis of Gracilaria with acid protease and trypsin; it contains rich Gracilaria hydrolyzed peptides, which can dilate blood vessels, reduce peripheral resistance and lower blood pressure by regulating the contraction and relaxation of vascular smooth muscle; reduce the secretion of renin, inhibit the formation of angiotensin, and thus lower blood pressure; secrete insulin from pancreatic β cells, and increase the insulin level in the body; inhibit the biosynthesis of cholesterol in the liver, accelerate the decomposition and excretion of cholesterol, and reduce the cholesterol content in the blood. DETAILED DESCRIPTION

[0029] The present invention is further described below in conjunction with specific implementation cases, but the present invention is not limited to these embodiments.

[0030] Example 1

[0031] A composition for lowering blood sugar, blood lipids and blood pressure, the composition is made of the following raw materials in parts by weight: 5 parts of Cyclocarya paliurus flower extract, 1 part of chicken heart extract, 3.5 parts of Siniperca chuatsi head extract, and 1.2 parts of Gracilaria extract;

[0032] Among them, the preparation method of the Cyclocarya paliurus flower extract is as follows: fresh Cyclocarya paliurus flowers are crushed, 9 times the mass of water of the Cyclocarya paliurus flowers are added, 1% of the mass of the Cyclocarya paliurus flowers with an enzyme activity of 100,000 U / g hemicellulase and 1% of the mass of the Cyclocarya paliurus flowers with an enzyme activity of 100,000 U / g bromelain are added, enzymolysis is performed at 55°C for 1.5 hours, and the enzyme is inactivated at 75°C for 20 minutes. After cooling to room temperature, 1.5% of the mass of the Cyclocarya paliurus flowers with an enzyme activity of 100,000 U / g ficin is added, enzymolysis is performed at 65°C for 1 hour, and the enzyme is inactivated at 75°C for 20 minutes. After cooling to room temperature, a 3kDa ultrafiltration membrane is used for filtration to obtain a filtrate below 3 kDa, and spray drying is performed to obtain the Cyclocarya paliurus flower extract.

[0033] The preparation method of the chicken heart extract is as follows: crush the fresh chicken heart, wash it 4 times with an ethanol aqueous solution with a volume concentration of 25%, and the amount of the ethanol aqueous solution used for each washing is 5 times the mass of the chicken heart, and finally filter to obtain a solid; then add 9 times the mass of water to the solid, adjust the pH to 1.8 with hydrochloric acid, add 1.8% of the solid mass of pepsin with an enzyme activity of 10,000 U / g, enzymolyze at 37°C for 2.5 hours, inactivate the enzyme at 85°C for 20 minutes, cool to room temperature, adjust the pH to 8.2, add 1.2% of the solid mass of subtilisin with an enzyme activity of 30,000 U / g, enzymolyze at 45°C for 1 hour, inactivate the enzyme at 85°C for 20 minutes, cool to room temperature, filter with a 5kDa ultrafiltration membrane to obtain a filtrate below 5kDa, spray dry, and obtain the chicken heart extract.

[0034] The preparation method of the mandarin fish head extract is as follows: crush the mandarin fish head without gills, wash it 4 times with an ethanol aqueous solution with a volume concentration of 25%, and the amount of the ethanol aqueous solution used for each washing is 5 times the mass of the mandarin fish head, and finally filter to obtain a solid; then add 9 times the mass of water to the solid, adjust the pH to 5, add 1.5% of the solid mass of 5 billion CFU / g Aspergillus niger, ferment at 35°C for 9d, sterilize at 122°C for 15min, cool to room temperature, adjust the pH to 8.1, add 0.8% of the solid mass of 100,000 U / g trypsin, enzymolyze at 37°C for 2.5h, inactivate the enzyme at 75°C for 20min, cool to room temperature, filter with a 3kDa ultrafiltration membrane to obtain a filtrate below 3kDa, spray dry, and obtain the mandarin fish head extract.

[0035] The preparation method of the Gracilaria extract is as follows: crush the Gracilaria, add a 10% sodium hydroxide aqueous solution with a volume concentration of 11 times the mass of the Gracilaria, mix at 65°C for 2 hours, filter, and obtain a solid; add water with a volume concentration of 9 times the mass of the solid to the solid, adjust the pH to 2.8, add 1% of the solid mass of 100,000 U / g acid protease, enzymolyze at 50°C for 1 hour, inactivate the enzyme at 75°C for 20 minutes, cool to room temperature, adjust the pH to 8.2, add 0.8% of the solid mass of 100,000 U / g trypsin, enzymolyze at 38°C for 1 hour, inactivate the enzyme at 75°C for 20 minutes, cool to room temperature, filter with a 5kDa ultrafiltration membrane, obtain a filtrate below 5kDa, spray dry, and obtain the Gracilaria extract.

[0036] The preparation method of the above-mentioned composition for lowering blood sugar, blood lipids and blood pressure comprises the following steps:

[0037] S1: uniformly mixing the Cyclocarya paliurus flower extract, the Gastrodia elata extract, the Siniperca chuatsi extract and the Gracilaria truncatula extract according to the mass ratio to obtain a composition;

[0038] S2: Mixing the composition with pharmaceutically acceptable excipients to prepare tablets or capsules.

[0039] Example 2

[0040] A composition for lowering blood sugar, blood lipids and blood pressure, the composition is made of the following raw materials in parts by weight: 3 parts of Cyclocarya paliurus flower extract, 0.5 parts of chicken heart extract, 2 parts of Siniperca chuatsi head extract, and 0.5 parts of Gracilaria extract;

[0041] Among them, the preparation method of the Cyclocarya paliurus flower extract is as follows: fresh Cyclocarya paliurus flowers are crushed, water of 8 times the mass of the Cyclocarya paliurus flowers is added, 0.5% of the mass of the Cyclocarya paliurus flowers with an enzyme activity of 100,000 U / g hemicellulase and 0.5% of the mass of the Cyclocarya paliurus flowers with an enzyme activity of 100,000 U / g bromelain are added, enzymolysis is performed at 50°C for 1.5 hours, the enzyme is inactivated at 70°C for 15 minutes, and after cooling to room temperature, 1% of the mass of the Cyclocarya paliurus flowers with an enzyme activity of 100,000 U / g ficin is added, enzymolysis is performed at 60°C for 1 hour, the enzyme is inactivated at 70°C for 15 minutes, and after cooling to room temperature, a 3kDa ultrafiltration membrane is used for filtration to obtain a filtrate below 3 kDa, and spray drying is performed to obtain the Cyclocarya paliurus flower extract.

[0042] The preparation method of the chicken heart extract is as follows: crush the fresh chicken heart, wash it three times with an ethanol aqueous solution with a volume concentration of 20%, and the amount of the ethanol aqueous solution used for each washing is 4 times the weight of the chicken heart, and finally filter to obtain a solid; then add water 8 times the weight of the solid to the solid, adjust the pH to 1.5 with hydrochloric acid, add 1.5% of the weight of the solid with an enzyme activity of 10,000 U / g pepsin, enzymatically hydrolyze at 36°C for 2 hours, and then add 10,000 U / g pepsin with an enzyme activity of 10,000 U / g.

[0043] The enzyme was inactivated at 0°C for 15 min, and after cooling to room temperature, the pH was adjusted to 7.8, and 0.8% by weight of solid subtilisin with an enzyme activity of 30,000 U / g was added. The enzyme was hydrolyzed at 40°C for 1 h, and the enzyme was inactivated at 80°C for 15 min. After cooling to room temperature, it was filtered using a 5 kDa ultrafiltration membrane to obtain a filtrate below 5 kDa, which was spray-dried to obtain a chicken heart extract.

[0044] Among them, the preparation method of the mandarin fish head extract is as follows: crush the mandarin fish head without fish gills, wash it three times with a volume concentration of 20% ethanol aqueous solution, the amount of ethanol aqueous solution used for each washing is 4 times the mass of the mandarin fish head, and finally filter to obtain a solid; then add 8 times the mass of water to the solid, adjust the pH to 4, add 1% of the solid mass of 5 billion CFU / g Aspergillus niger, ferment at 30°C for 8d, sterilize at 121°C for 15min, cool to room temperature, adjust the pH to 7.8, add 0.5% of the solid mass of 100,000 U / g trypsin, enzymolyze at 35°C for 2h, inactivate the enzyme at 70°C for 15min, cool to room temperature, filter with a 3kDa ultrafiltration membrane to obtain a filtrate below 3kDa, spray dry, and obtain the mandarin fish head extract.

[0045] The preparation method of the Gracilaria extract is as follows: crush the Gracilaria, add an 8% sodium hydroxide aqueous solution with a volume concentration of 10 times the mass of the Gracilaria, mix at 60°C for 1 hour, filter, and obtain a solid; add water with a volume concentration of 8% of the mass of the Gracilaria to the solid, adjust the pH to 2.5, add 0.5% of the mass of the solid with an enzyme activity of 100,000 U / g acid protease, hydrolyze at 40°C for 1 hour, inactivate the enzyme at 70°C for 15 minutes, cool to room temperature, adjust the pH to 7.8, add 0.5% of the mass of the solid with an enzyme activity of 100,000 U / g trypsin, hydrolyze at 35°C for 1 hour, inactivate the enzyme at 70°C for 15 minutes, cool to room temperature, filter with a 5kDa ultrafiltration membrane, obtain a filtrate below 5kDa, spray dry, and obtain the Gracilaria extract.

[0046] The preparation method of the above-mentioned composition for lowering blood sugar, blood lipids and blood pressure comprises the following steps:

[0047] S1: uniformly mixing the Cyclocarya paliurus flower extract, the Gastrodia elata extract, the Siniperca chuatsi extract and the Gracilaria truncatula extract according to the mass ratio to obtain a composition;

[0048] S2: Mixing the composition with pharmaceutically acceptable excipients to prepare tablets or capsules.

[0049] Example 3

[0050] A composition for lowering blood sugar, blood lipids and blood pressure, the composition is made of the following raw materials in parts by weight: 3 parts of Cyclocarya paliurus flower extract, 1.5 parts of chicken heart extract, 2 parts of Siniperca chuatsi head extract, and 1.5 parts of Gracilaria extract;

[0051] Among them, the preparation method of the Cyclocarya paliurus flower extract is as follows: fresh Cyclocarya paliurus flowers are crushed, water of 8 times the mass of the Cyclocarya paliurus flowers is added, 1.5% of the mass of the Cyclocarya paliurus flowers is added with an enzyme activity of 100,000 U / g hemicellulase and 0.5% of the mass of the Cyclocarya paliurus flowers is added with an enzyme activity of 100,000 U / g bromelain, enzymolysis is performed at 60°C for 1.5 hours, the enzyme is inactivated at 80°C for 15 minutes, and after cooling to room temperature, 2% of the mass of the Cyclocarya paliurus flowers is added with an enzyme activity of 100,000 U / g ficin, enzymolysis is performed at 60°C for 1.5 hours, the enzyme is inactivated at 70°C for 25 minutes, and after cooling to room temperature, a 3kDa ultrafiltration membrane is used for filtration to obtain a filtrate below 3kDa, and the filtrate is spray-dried to obtain the Cyclocarya paliurus flower extract.

[0052] Among them, the preparation method of chicken heart extract is: crush fresh chicken hearts, wash them 5 times with ethanol aqueous solution with a volume concentration of 20%, the amount of ethanol aqueous solution used for each washing is 4 times the mass of the chicken heart, and finally filter to obtain a solid; then add water 10 times the mass of the solid to the solid, adjust the pH to 1.5 with hydrochloric acid, add 2% of the solid mass of pepsin with an enzyme activity of 10,000 U / g, hydrolyze at 36°C for 3h, inactivate the enzyme at 80°C for 25min, cool to room temperature, adjust the pH to 7.8, add 1.5% of the solid mass of subtilisin with an enzyme activity of 30,000 U / g, hydrolyze at 40°C for 1.5h, inactivate the enzyme at 80°C for 25min, cool to room temperature, filter with a 5kDa ultrafiltration membrane to obtain a filtrate below 5kDa, spray dry, and obtain a chicken heart extract.

[0053] Among them, the preparation method of the mandarin fish head extract is as follows: crush the mandarin fish head without gills, wash it 5 times with a volume concentration of 20% ethanol aqueous solution, the amount of ethanol aqueous solution used for each washing is 4 times the mass of the mandarin fish head, and finally filter to obtain a solid; then add 10 times the mass of water to the solid, adjust the pH to 4, add 2% of the solid mass of 5 billion CFU / g Aspergillus niger, ferment at 30°C for 10 days, sterilize at 121°C for 20 minutes, cool to room temperature, adjust the pH to 7.8, add 1% of the solid mass of 100,000 U / g trypsin, enzymolyze at 35°C for 3 hours, inactivate the enzyme at 70°C for 25 minutes, cool to room temperature, filter with a 3kDa ultrafiltration membrane to obtain a filtrate below 3kDa, spray dry, and obtain the mandarin fish head extract.

[0054] The preparation method of the Gracilaria extract is as follows: crush the Gracilaria, add a sodium hydroxide aqueous solution with a volume concentration of 12% in an amount 10 times the mass of the Gracilaria, mix at 60°C for 3 hours, filter, and obtain a solid; add water with a mass 8 times the mass of the solid to the solid, adjust the pH to 3.0, add 0.5% of the solid mass of 100,000 U / g acid protease, enzymolyze at 60°C for 1 hour, inactivate the enzyme at 80°C for 15 minutes, cool to room temperature, adjust the pH to 8.5, add 0.5% of the solid mass of 100,000 U / g trypsin, enzymolyze at 40°C for 1 hour, inactivate the enzyme at 80°C for 15 minutes, cool to room temperature, filter using a 5kDa ultrafiltration membrane to obtain a filtrate below 5kDa, and spray dry to obtain the Gracilaria extract.

[0055] The preparation method of the above-mentioned composition for lowering blood sugar, blood lipids and blood pressure comprises the following steps:

[0056] S1: uniformly mixing the Cyclocarya paliurus flower extract, the Gastrodia elata extract, the Siniperca chuatsi extract and the Gracilaria truncatula extract according to the mass ratio to obtain a composition;

[0057] S2: Mixing the composition with pharmaceutically acceptable excipients to prepare tablets or capsules.

[0058] Example 4

[0059] A composition for lowering blood sugar, blood lipids and blood pressure, the composition is made of the following raw materials in parts by weight: 5 parts of Cyclocarya paliurus flower extract, 0.8 parts of chicken heart extract, 3.5 parts of Siniperca chuatsi head extract, and 0.8 parts of Gracilaria extract;

[0060] Among them, the preparation method of the Cyclocarya paliurus flower extract is as follows: fresh Cyclocarya paliurus flowers are crushed, water of 8 times the mass of the Cyclocarya paliurus flowers is added, 0.8% of the mass of the Cyclocarya paliurus flowers is added with an enzyme activity of 100,000 U / g hemicellulase and 1.2% of the mass of the Cyclocarya paliurus flowers is added with an enzyme activity of 100,000 U / g bromelain, enzymolysis is performed at 55°C for 1.5 hours, the enzyme is inactivated at 80°C for 15 minutes, and after cooling to room temperature, 1.8% of the mass of the Cyclocarya paliurus flowers is added with an enzyme activity of 100,000 U / g ficin, enzymolysis is performed at 70°C for 1 hour, the enzyme is inactivated at 80°C for 15 minutes, and after cooling to room temperature, a 3kDa ultrafiltration membrane is used for filtration to obtain a filtrate below 3kDa, and the filtrate is spray-dried to obtain the Cyclocarya paliurus flower extract.

[0061] The preparation method of the chicken heart extract is as follows: crush the fresh chicken heart, wash it 5 times with an ethanol aqueous solution with a volume concentration of 20%, and the amount of the ethanol aqueous solution used for each washing is 4 times the mass of the chicken heart, and finally filter to obtain a solid; then add water 8 times the mass of the solid to the solid, adjust the pH to 1.6 with hydrochloric acid, add 1.6% of the solid mass of pepsin with an enzyme activity of 10,000 U / g, enzymolyze at 37°C for 2.5 hours, inactivate the enzyme at 80°C for 20 minutes, cool to room temperature, adjust the pH to 8, add 1.2% of the solid mass of subtilisin with an enzyme activity of 30,000 U / g, enzymolyze at 48°C for 1 hour, inactivate the enzyme at 80°C for 20 minutes, cool to room temperature, filter with a 5kDa ultrafiltration membrane to obtain a filtrate below 5kDa, spray dry, and obtain the chicken heart extract.

[0062] Among them, the preparation method of the mandarin fish head extract is as follows: crush the mandarin fish head without fish gills, wash it three times with a volume concentration of 30% ethanol aqueous solution, the amount of ethanol aqueous solution used for each washing is 6 times the mass of the mandarin fish head, and finally filter to obtain a solid; then add 8 times the mass of water to the solid, adjust the pH to 5.5, add 1% of the solid mass of 5 billion CFU / g Aspergillus niger, ferment at 37°C for 8 days, sterilize at 125°C for 15 minutes, cool to room temperature, adjust the pH to 8.5, add 0.5% of the solid mass of 100,000 U / g trypsin, enzymolyze at 37°C for 2 hours, inactivate the enzyme at 70°C for 15 minutes, cool to room temperature, filter with a 3kDa ultrafiltration membrane to obtain a filtrate below 3kDa, spray dry, and obtain the mandarin fish head extract.

[0063] The preparation method of the Gracilaria extract is as follows: crush the Gracilaria, add a 10% sodium hydroxide aqueous solution with a volume concentration of 12 times the mass of the Gracilaria, mix at 60°C for 2.5 hours, filter, and obtain a solid; add water with a volume concentration of 10 times the mass of the solid to the solid, adjust the pH to 2.8, add 0.8% of the solid mass of 100,000 U / g acid protease, enzymolyze at 55°C for 1 hour, inactivate the enzyme at 75°C for 25 minutes, cool to room temperature, adjust the pH to 8.2, add 1% of the solid mass of 100,000 U / g trypsin, enzymolyze at 37°C for 1.5 hours, inactivate the enzyme at 70°C for 20 minutes, cool to room temperature, filter using a 5kDa ultrafiltration membrane to obtain a filtrate below 5kDa, spray dry, and obtain the Gracilaria extract.

[0064] The preparation method of the above-mentioned composition for lowering blood sugar, blood lipids and blood pressure comprises the following steps:

[0065] S1: uniformly mixing the Cyclocarya paliurus flower extract, the Gastrodia elata extract, the Siniperca chuatsi extract and the Gracilaria truncatula extract according to the mass ratio to obtain a composition;

[0066] S2: Mixing the composition with pharmaceutically acceptable excipients to prepare tablets or capsules.

[0067] Example 5

[0068] A composition for lowering blood sugar, blood lipids and blood pressure, the composition is made of the following raw materials in parts by weight: 6 parts of Cyclocarya paliurus flower extract, 1.5 parts of chicken heart extract, 5 parts of Siniperca chuatsi head extract, and 1.5 parts of Gracilaria extract;

[0069] Among them, the preparation method of the Cyclocarya paliurus flower extract is as follows: fresh Cyclocarya paliurus flowers are crushed, 10 times the mass of water of the Cyclocarya paliurus flowers are added, 1.5% of the mass of the Cyclocarya paliurus flowers with an enzyme activity of 100,000 U / g hemicellulase and 1.5% of the mass of the Cyclocarya paliurus flowers with an enzyme activity of 100,000 U / g bromelain are added, enzymolysis is performed at 60°C for 2h, the enzyme is inactivated at 80°C for 25min, and after cooling to room temperature, 2% of the mass of the Cyclocarya paliurus flowers with an enzyme activity of 100,000 U / g ficin are added, enzymolysis is performed at 70°C for 1.5h, the enzyme is inactivated at 80°C for 25min, and after cooling to room temperature, a 3kDa ultrafiltration membrane is used for filtration to obtain a filtrate below 3kDa, and the filtrate is spray-dried to obtain the Cyclocarya paliurus flower extract.

[0070] The preparation method of the chicken heart extract is as follows: crush the fresh chicken heart, wash it 5 times with an ethanol aqueous solution with a volume concentration of 30%, and the amount of the ethanol aqueous solution used for each washing is 6 times the mass of the chicken heart, and finally filter to obtain a solid; then add water 10 times the mass of the solid to the solid, adjust the pH to 2 with hydrochloric acid, add 2% of the solid mass of pepsin with an enzyme activity of 10,000 U / g, enzymolyze at 40°C for 3h, inactivate the enzyme at 90°C for 25min, and after cooling to room temperature, adjust the pH to 8.5, add 1.5% of the solid mass of subtilisin with an enzyme activity of 30,000 U / g, enzymolyze at 50°C for 1.5h, inactivate the enzyme at 90°C for 25min, and after cooling to room temperature, filter with a 5kDa ultrafiltration membrane to obtain a filtrate below 5kDa, spray dry, and obtain the chicken heart extract.

[0071] Among them, the preparation method of the mandarin fish head extract is as follows: crush the mandarin fish head without fish gills, wash it 5 times with a volume concentration of 30% ethanol aqueous solution, the amount of ethanol aqueous solution used for each washing is 6 times the mass of the mandarin fish head, and finally filter to obtain a solid; then add 10 times the mass of water of the solid to the solid, adjust the pH to 5.5, add 2% of the solid mass of 5 billion CFU / g Aspergillus niger, ferment at 37°C for 10 days, sterilize at 125°C for 20 minutes, cool to room temperature, adjust the pH to 8.5, add 1% of the solid mass of 100,000 U / g trypsin, enzymolyze at 38°C for 3 hours, inactivate the enzyme at 80°C for 25 minutes, cool to room temperature, filter with a 3kDa ultrafiltration membrane to obtain a filtrate below 3kDa, spray dry, and obtain the mandarin fish head extract.

[0072] The preparation method of the Gracilaria extract is as follows: crush the Gracilaria, add a sodium hydroxide aqueous solution with a volume concentration of 12% at 12 times the mass of the Gracilaria, mix at 70°C for 3 hours, filter, and obtain a solid; add water with a volume concentration of 12% at 12 times the mass of the Gracilaria to the solid, adjust the pH to 3.0, add 1.5% of the mass of the solid with an enzyme activity of 100,000 U / g acid protease, enzymolyze at 60°C for 1.5 hours, inactivate the enzyme at 80°C for 25 minutes, cool to room temperature, adjust the pH to 8.5, add 1% of the mass of the solid with an enzyme activity of 100,000 U / g trypsin, enzymolyze at 40°C for 1.5 hours, inactivate the enzyme at 80°C for 25 minutes, cool to room temperature, filter using a 5kDa ultrafiltration membrane to obtain a filtrate below 5kDa, and spray dry to obtain the Gracilaria extract.

[0073] The preparation method of the above-mentioned composition for lowering blood sugar, blood lipids and blood pressure comprises the following steps:

[0074] S1: uniformly mixing the Cyclocarya paliurus flower extract, the Gastrodia elata extract, the Siniperca chuatsi extract and the Gracilaria truncatula extract according to the mass ratio to obtain a composition;

[0075] S2: Mixing the composition with pharmaceutically acceptable excipients to prepare tablets or capsules.

[0076] Comparative Example 1

[0077] No Cyclocarya paliurus flower extract is added to the composition, and the Cyclocarya paliurus flower extract component is replaced by starch; other parameters and methods are the same as in Example 1.

[0078] Comparative Example 2

[0079] No chicken heart extract was added to the composition, and other parameters and methods were the same as in Example 1.

[0080] Comparative Example 3

[0081] No mandarin fish head extract was added to the composition, and other parameters and methods were the same as in Example 1.

[0082] Comparative Example 4

[0083] No Gracilaria extract was added to the composition, and other parameters and methods were the same as in Example 1.

[0084] Comparative Example 5

[0085] In the preparation method of the Cyclocarya paliurus flower extract, no ficin hydrolysis is performed, and other parameters and methods are the same as in Example 1.

[0086] Comparative Example 6

[0087] In the preparation method of the Cyclocarya paliurus flower extract, subtilisin enzymolysis is not performed, and other parameters and methods are the same as in Example 1.

[0088] Comparative Example 7

[0089] In the preparation method of the mandarin fish head extract, no Aspergillus niger fermentation is performed, and trypsin enzymolysis is performed directly. Other parameters and methods are the same as in Example 1.

[0090] Comparative Example 8

[0091] In the preparation method of Gracilaria extract, trypsin hydrolysis is not performed, and other parameters and methods are the same as in Example 1.

[0092] The compositions of the above-mentioned embodiments and comparative examples were tested experimentally.

[0093] 1. Toxicity test:

[0094] Six mice (half male and half female) were selected in each group for acute oral toxicity test: the compositions of each embodiment and each comparative example were respectively administered intragastrically at a dose of 0.5 g / kg·bw, twice a day, and the physical signs were observed. Result: negative, belonging to the non-toxic grade.

[0095] Six mice (half male and half female) were selected for each group for feeding for 30 days: the composition of each embodiment and each comparative example was taken respectively, and three dosage groups were set at 0.5g / kg, 1g / kg, and 2g / kg, and a blank control group without adding the composition was set up. The composition samples were mixed into the basic feed and fed to mice for 30 consecutive days. The symptoms of poisoning and death were observed every day. At the end of the experiment, the hematology, blood biochemistry, and organ coefficient indicators were observed, and pathological examinations were performed. The results showed that the indicators were all within the normal range and safe and non-toxic.

[0096] 2. Animal Experiments on Hypoglycemic Effects:

[0097] High sugar feed formula: 66.5% basic feed + 10% lard + 20% sucrose + 2.5% cholesterol + 1% sodium cholate. Basic feed: 73.5% corn, 20% wheat bran, 5% fish meal, 1% cereal flour, 0.5% salt.

[0098] 1% streptozotocin solution: Prepare a 1% streptozotocin solution using pH 4 citric acid-sodium citrate buffer.

[0099] Modeling grouping: Rats were fed with basic feed for one week at an ambient temperature of 22°C and a relative humidity of 45%, and then the following operations were performed:

[0100] Eight rats (half male and half female) were fed with basal diet for 4 weeks, fasted for 12 h, and intraperitoneally injected with 0.375 g / kg normal saline to serve as the normal control group;

[0101] Several rats were fed with high-sugar and high-fat diet for 4 weeks, fasted for 12 hours, and intraperitoneally injected with 1% streptozotocin solution at 30 mg / kg to prepare a rat diabetes model; after the injection, the high-sugar diet was continued for 1 week, and the fasting blood sugar was measured. The blood sugar>11mmo l / L was considered to be a successful model. 104 rats with successful modeling were divided into 13 groups (8 rats in each group, half male and half female), corresponding to the treatment groups of the compositions of each embodiment and each comparative example; 8 rats with successful modeling (half male and half female) were taken, corresponding to the model group; 8 rats with successful modeling (half male and half female) were taken, the positive drug group;

[0102] (1) Treatment group: The patients were given the compositions prepared in the examples and comparative examples, with a daily intragastric administration dose of 0.2 g / kg; and continued to be given high-sugar feed;

[0103] (2) Positive drug group: daily gavage of metformin 0.2 g / kg; continued to be fed high-sugar feed;

[0104] (3) Model group: given 0.2 g / kg normal saline every day; continued to be given high-sugar feed;

[0105] (4) Normal control group: given 0.2 g / kg normal saline every day; continued to be given basic feed;

[0106] The rats were fasted but not watered for 12 h 1 day before oral administration and on the 15th day after oral administration, and then their tails were cut to collect blood, and their fasting blood glucose levels were measured using a blood glucose analyzer.

[0107] The test results are shown in Table 1 below:

[0108] Table 1 Results of hypoglycemic animal experiments (average values)

[0109]

[0110] From the above results, it can be seen that the combination of Cyclocarya paliurus flower extract, chicken heart extract, mandarin fish head extract and Gracilaria extract has a very excellent hypoglycemic effect; among them, after omitting the preparation steps of Cyclocarya paliurus flower extract, chicken heart extract, mandarin fish head extract and Gracilaria extract, the hypoglycemic effect of each will be greatly reduced, or even almost no hypoglycemic effect. Without adding any component of Cyclocarya paliurus flower extract, chicken heart extract, mandarin fish head extract and Gracilaria extract, the hypoglycemic effect is greatly reduced.

[0111] 3. Animal experiments on lowering blood lipids:

[0112] High-fat feed: 90.1% basic feed, 1.5% cholesterol, 8.2% lard and 0.

[0113] 2%. Basic feed: corn 73.5%, wheat bran 20%, fish meal 5%, cereal powder 1%, salt 0.5%.

[0114] Several mice weighing 20-25g were selected and fed with basic feed for one week; 8 mice (more than half of them were males and females) were taken to continue feeding with basic feed as the blank control group; the remaining mice were fed with high-fat feed, 3 times a day, 2g each time, and drank normal water during the period, and the feeding was continued for 14 days to establish the model.

[0115] 104 mice with successful modeling were divided into 13 groups, each with 8 mice (half male and half female), and each group corresponded to the treatment group of the composition of each embodiment and each comparative example; 8 rats with successful modeling (half male and half female) were taken as the model group; 8 rats with successful modeling (half male and half female) were taken as the positive drug group;

[0116] (1) Treatment group: The patients were given the compositions prepared in the examples and comparative examples, with a daily oral administration dose of 0.2 g / kg; and continued to be fed with high-fat feed;

[0117] (2) Positive drug group: daily oral administration of simvastatin 0.2 g / kg; continued to be fed high-fat diet;

[0118] (3) Model group: given 0.2 g / kg normal saline every day; continued to be given high-fat diet;

[0119] (4) Normal control group: given 0.2 g / kg normal saline every day; continued to be given basic feed;

[0120] The mice were fasted for 12 h for 40 days, blood was collected from the orbital vein, and serum was separated by centrifugation to measure triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDLC).

[0121] The test results are shown in Table 2 below:

[0122] Table 2 Results of animal experiments on reducing blood lipids (average values)

[0123]

[0124]

[0125] From the above results, it can be seen that the combination of Cyclocarya paliurus flower extract, chicken heart extract, mandarin fish head extract and Gracilaria extract has a good effect on improving blood lipids. Among them, after omitting the preparation steps of Cyclocarya paliurus flower extract, chicken heart extract, mandarin fish head extract and Gracilaria extract, their respective blood lipid lowering effects will be greatly reduced, or even almost have no blood lipid lowering effect. Without adding any component of Cyclocarya paliurus flower extract, chicken heart extract, mandarin fish head extract and Gracilaria extract, the blood lipid lowering effect is greatly reduced.

[0126] 4. Animal experiments on lowering blood pressure:

[0127] 120 male rats with essential hypertension (systolic blood pressure of arterial blood pressure is 155 mmHg ~ 162 mmHg) were selected, and were normally raised for one week, and were evenly divided into 15 groups, each with 8 rats (half male and half female), corresponding to the treatment group, model group and positive drug group of each embodiment and comparative example composition.

[0128] (1) Treatment group: The composition prepared in Example and Comparative Example was administered orally at a daily dose of 0.2 g / kg;

[0129] (2) Positive drug group: daily oral administration of nifedipine 9 mg / kg;

[0130] (3) Model group: intragastrically administered 0.2 g / kg normal saline every day

[0131] Before treatment and after 30 days of treatment, arterial blood pressure was measured, and the test results are shown in Table 3 below:

[0132] Table 3 Results of animal experiments on lowering blood pressure (average value)

[0133]

[0134]

[0135] From the above results, it can be seen that the combination of Cyclocarya paliurus flower extract, chicken heart extract, mandarin fish head extract and Gracilaria extract has a good blood pressure lowering effect; among them, after omitting the preparation steps of Cyclocarya paliurus flower extract, chicken heart extract, mandarin fish head extract and Gracilaria extract, their respective lipid-lowering effects will be greatly reduced. Without adding any component of Cyclocarya paliurus flower extract, chicken heart extract, mandarin fish head extract and Gracilaria extract, the blood pressure lowering effect is greatly reduced.

Claims

1. A composition for lowering blood sugar, blood lipids and blood pressure, characterized in that: The composition is prepared from the following raw materials in parts by weight: 3 to 6 parts of Cyclocarya paliurus flower extract, 0.5 to 1.5 parts of chicken heart extract, 2 to 5 parts of mandarin fish head extract, and 0.5 to 1.5 parts of Gracilaria extract; The preparation method of the Cyclocarya paliurus flower extract comprises: crushing fresh Cyclocarya paliurus flowers, adding water 8 to 10 times the mass of the Cyclocarya paliurus flowers, adding hemicellulase 0.5 to 1.5% by mass of the Cyclocarya paliurus flowers and bromelain 0.5 to 1.5% by mass of the Cyclocarya paliurus flowers, performing enzymolysis at 50 to 60° C. for 1.5 to 2 hours, inactivating the enzyme at high temperature, cooling to room temperature, adding 1 to 2% by mass of ficin at 60 to 70° C. for 1 hour to 1.5 hours, inactivating the enzyme at high temperature, cooling to room temperature, filtering with a 3 kDa ultrafiltration membrane to obtain a filtrate below 3 kDa, and spray drying to obtain the Cyclocarya paliurus flower extract; The preparation method of the chicken heart extract is as follows: fresh chicken hearts are crushed, washed with ethanol aqueous solution for 3 to 5 times, and finally filtered to obtain a solid; then 8 to 10 times the weight of water is added to the solid, the pH is adjusted to 1.5 to 2 with hydrochloric acid, 1.5% to 2% of pepsin by weight of the solid is added, enzymolysis is carried out at 36°C to 40°C for 2h to 3h, the enzyme is inactivated at high temperature, the pH is adjusted to 7.8 to 8.5 after the pH is cooled to room temperature, 0.8% to 1.5% of subtilisin by weight of the solid is added, enzymolysis is carried out at 40°C to 50°C for 1h to 1.5h, the enzyme is inactivated at high temperature, the pH is cooled to room temperature, and a 5kDa ultrafiltration membrane is used for filtering to obtain a filtrate below 5kDa, and the filtrate is spray-dried to obtain a chicken heart extract; The preparation method of the mandarin fish head extract is as follows: crushing the mandarin fish head, washing it with an ethanol aqueous solution for 3 to 5 times, and finally filtering to obtain a solid; then adding water 8 to 10 times the weight of the solid to the solid, adjusting the pH to 4 to 5.5, adding 1% to 2% of the weight of the solid Aspergillus niger, fermenting at 30 to 37° C. for 8 to 10 days, sterilizing at high temperature, cooling to room temperature, adjusting the pH to 7.8 to 8.5, adding 0.5% to 1% of the weight of the solid trypsin, enzymolyzing at 35 to 38° C. for 2 hours to 3 hours, inactivating the enzyme at high temperature, cooling to room temperature, filtering with a 3 kDa ultrafiltration membrane to obtain a filtrate below 3 kDa, and spray drying to obtain the mandarin fish head extract; The preparation method of the Gracilaria extract is as follows: taking Gracilaria and crushing it, adding a sodium hydroxide aqueous solution with a mass of 10 to 12 times that of Gracilaria, mixing at 60 to 70° C. for 1 hour to 3 hours, filtering to obtain a solid; adding water with a mass of 8 to 10 times that of the solid to the solid, adjusting the pH to 2.5 to 3.0, adding an acid protease with a mass of 0.5% to 1.5% of the solid mass, performing enzymolysis at 40 to 60° C. for 1 hour to 1.5 hours, inactivating the enzyme at high temperature, cooling to room temperature, adjusting the pH to 7.8 to 8.5, adding trypsin with a mass of 0.5% to 1% of the solid mass, performing enzymolysis at 35 to 40° C. for 1 hour to 1.5 hours, inactivating the enzyme at high temperature, cooling to room temperature, filtering with a 5 kDa ultrafiltration membrane to obtain a filtrate below 5 kDa, spray drying, and obtaining the Gracilaria extract.

2. A composition for lowering blood sugar, blood lipids and blood pressure according to claim 1, characterized in that: In the preparation method of Cyclocarya paliurus flower extract, the enzymatic activity of the hemicellulase is 100,000 U / g; the enzymatic activity of the bromelain is 100,000 U / g; the enzymatic activity of the ficin is 100,000 U / g; the temperature of the high-temperature enzyme inactivation is 70°C to 80°C, and the time is 15min to 25min.

3. A composition for lowering blood sugar, blood lipids and blood pressure according to claim 1, characterized in that: In the preparation method of chicken heart extract, the volume concentration of the ethanol aqueous solution is 20% to 30%; the enzyme activity of the pepsin is 10,000 U / g; the enzyme activity of the subtilisin is 30,000 U / g; the temperature of the high-temperature enzyme inactivation is 80°C to 90°C, and the time is 15min to 25min.

4. The composition for lowering blood sugar, blood lipids and blood pressure according to claim 1, characterized in that: In the preparation method of the mandarin fish head extract, the mandarin fish head does not contain fish gills; the volume concentration of the ethanol aqueous solution is 20% to 30%; the bacterial activity of the Aspergillus niger is 5 billion CFU / g; the enzyme activity of the trypsin is 100,000 U / g; the temperature of the high-temperature sterilization is 121°C to 125°C, and the time is 15min to 20min; the temperature of the high-temperature enzyme inactivation is 70°C to 80°C, and the time is 15min to 25min.

5. The composition for lowering blood sugar, blood lipids and blood pressure according to claim 1, characterized in that: In the preparation method of Gracilaria extract, the volume concentration of the sodium hydroxide aqueous solution is 8% to 12%, the enzyme activity of the acidic protease is 100,000 U / g; the enzyme activity of the trypsin is 100,000 U / g; the temperature of the high-temperature enzyme inactivation is 70°C to 80°C, and the time is 15min to 25min.

6. A method for preparing a composition for lowering blood sugar, blood lipids and blood pressure, which is used to prepare the composition for lowering blood sugar, blood lipids and blood pressure as claimed in claim 1, characterized in that: The preparation method comprises the following steps: S1: uniformly mixing the Cyclocarya paliurus flower extract, the Gastrodia elata extract, the Siniperca chuatsi extract and the Gracilaria truncatula extract according to the mass ratio to obtain a composition; S2: Mixing the composition with pharmaceutically acceptable excipients to prepare tablets or capsules.

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