Preparation method of a medicinal liquor and application of the medicinal liquor in preparation of products for preventing cardiovascular and cerebrovascular diseases and improving microcirculation

Abstract

The application provides a preparation method of medicinal liquor and application of the medicinal liquor in preparation of products for preventing cardiovascular and cerebrovascular diseases and improving microcirculation, and belongs to the technical field of pharmaceutical compositions. The preparation method comprises the following steps: (1) crushing; (2) extraction; (3) standing and separation; (4) freezing and filtration, so that the medicinal liquor is obtained. In the preparation process, the traditional Chinese medicine components are extracted through the steps of batch crushing, mixed extraction, standing, freezing and separation of supernatant and suspension by using different separation modes, so that the medicinal liquor with high active effective component activity and good stability is obtained. The medicinal liquor does not separate into layers and does not generate precipitate after long-term storage, and has remarkable effects in preventing cardiovascular and cerebrovascular diseases and improving microcirculation.

Description

Technical Field

[0001] This invention relates to the field of pharmaceutical composition technology, specifically to a method for preparing a medicinal wine and its application in the preparation of products for preventing cardiovascular and cerebrovascular diseases and improving microcirculation. Background Technology

[0002] Microcirculation is the site of metabolism in the human body, its internal environment, and a fundamental guarantee of life. Microcirculation includes arterioles, capillaries, arteriovenous anastomoses, venules, as well as lymphatic capillaries and the circulation of body fluids within tissues, corresponding to the concept of "meridians" in Traditional Chinese Medicine. The main function of microcirculation is to supply oxygen and nutrients to tissue cells and remove metabolic waste products such as carbon dioxide and lactic acid produced by cells. If microcirculation is impaired, blood perfusion will decrease, making it difficult to meet the needs of tissue oxidative metabolism. This can lead to functional decline in mild cases and, in severe cases, the development of many diseases.

[0003] Cardiovascular and cerebrovascular diseases are inextricably linked to microcirculatory disorders. The body's microcirculation directly participates in cellular metabolism and material exchange, supplying cells with blood, oxygen, energy, and nutrients, while also removing harmful metabolic byproducts such as creatine, lactic acid, and carbon dioxide from the body. If platelets, white blood cells, or fibrin in the microcirculation aggregate to form microthrombi, these thrombi can travel throughout the body with the bloodstream or deposit on the vascular endothelium. When these thrombi obstruct local blood flow, they can cause a series of symptoms. For example, when microcirculatory disorders occur in the nervous system, brain cells do not receive sufficient nutrients and oxygen, and cellular metabolic byproducts cannot be completely eliminated due to insufficient blood supply, leading to headaches, dizziness, insomnia, vivid dreams, and memory decline; in severe cases, stroke, hemiplegia, Alzheimer's disease, and peripheral neuritis may occur. When microcirculatory disorders occur in the cardiovascular system, they can cause chest tightness, palpitations, angina pectoris, and arrhythmia; in severe cases, myocardial infarction may occur. Microcirculatory disorders in other systems of the body can also cause corresponding symptoms. Therefore, improving microcirculation is beneficial to physical health and disease recovery, and the search for good methods to improve microcirculation has always been valued by the medical community.

[0004] Chinese Patent CN119157933A discloses a traditional Chinese medicine composition for promoting blood circulation and unblocking collaterals, and its application in the preparation of drugs for treating stroke. The composition is made from Astragalus membranaceus, Angelica sinensis (tail), Pheretima aspergillum, Spatholobus suberectus, Salvia miltiorrhiza, Carthamus tinctorius, Morus alba twig, Eupolyphaga sinensis, processed Polygonum multiflorum, Cornus officinalis, Morinda officinalis, and Eucommia ulmoides. This invention optimizes the combination of traditional formulas to obtain a novel traditional Chinese medicine formula with the effects of invigorating qi and promoting blood circulation, tonifying the liver and kidneys, and removing blood stasis and unblocking collaterals. It repairs brain damage caused by stroke, alleviates neurological deficits and brain tissue inflammation, is safe, and has no drug-induced liver toxicity. Combined with Western precision medicine, it significantly improves the treatment effect of stroke, alleviates patients' clinical symptoms, promotes the recovery of neurological function, and provides a new integrated traditional Chinese and Western medicine treatment strategy for clinical practice.

[0005] For example, Chinese patent CN104922397A discloses the application of a traditional Chinese medicine composition that promotes blood circulation, removes blood stasis, stops bleeding, and relieves pain. The composition is prepared from the following traditional Chinese medicine raw materials: Paris polyphylla, processed Aconitum carmichaelii, ginger peel, Datura stramonium, Smilax china, Ephedra sinica, Dioscorea opposita, Dioscorea opposita, Hedyotis diffusa, Datura stramonium, Strychnos nux-vomica, and Datura stramonium. The traditional Chinese medicine composition of this invention has the advantages of reasonable and unique prescription, simple process, wide clinical application and high market value in terms of prescription, processing technology and application.

[0006] However, existing Chinese medicine compositions are not very effective in improving microcirculation and have no significant therapeutic effect. Therefore, it is necessary to develop a method for preparing a medicinal wine that has significant effects in preventing cardiovascular and cerebrovascular diseases and improving microcirculation. Summary of the Invention

[0007] To achieve the above objectives, the present invention employs the following technical solution:

[0008] This invention provides a method for preparing medicinal wine, comprising the following steps:

[0009] (1) Crushing:

[0010] First, mix and grind the following ingredients: Amomum villosum, cinnamon, white cardamom, red cardamom, long pepper, and agarwood. Then add the following ingredients: prepared Polygonum multiflorum, Rehmannia glutinosa, Angelica dahurica, Dioscorea opposita, Galla chinensis, Pogostemon cablin, ginseng, Morus alba root bark, Erythrina variegata bark, Nardostachys chinensis, Angelica pubescens, Atractylodes lancea, Ligusticum chuanxiong, Cuscuta chinensis, Poria cocos, Citrus reticulata peel, Amomum tsao-ko, Cornus officinalis, Aconitum carmichaelii, Magnolia officinalis, Citrus reticulata peel, Schisandra chinensis, Achyranthes bidentata, Citrus aurantium, Alpinia officinarum, and Coltsfoot flower, fennel, platycodon root, prepared rehmannia root, acorus root, atractylodes rhizome, areca nut, licorice root, angelica root, gentian root, safflower, turmeric root, lotus seed, papaya, ophiopogon root, notopterygium root, cyperus rhizome, cistanche, astragalus root, asparagus root, peach kernel, gardenia fruit, alisma rhizome, lindera root, pinellia tuber, arisaema rhizome, bitter almond, poria cocos, polygala root, epimedium, sparganium rhizome, and madder root are mixed and pulverized to obtain a mixture.

[0011] (2) Extraction:

[0012] The liquor, red yeast rice, and other ingredients are mixed, heated to a boil, and then cooled to obtain a mixture.

[0013] (3) Settling and separation:

[0014] Take the medicine solution, seal it and let it stand. During the standing process, compressed air needs to be introduced and stirred. After the standing is completed, separate the supernatant and the suspension to obtain the medicine solution.

[0015] (4) Freezing and filtration:

[0016] The medicinal liquid is frozen and stored, and then filtered to obtain the medicinal wine.

[0017] Preferably, the particle size of the mixture in step (1) above is 24-50 mesh.

[0018] Preferably, in step (1) above, the yam is stir-fried yam; the Atractylodes lancea is stir-fried Atractylodes lancea; the Cuscuta chinensis is salt-fried Cuscuta chinensis; the Citrus reticulata peel is stir-fried Citrus reticulata peel; the Cornus officinalis is cornus officinalis without pit; the Aconitum carmichaelii is processed Aconitum carmichaelii; the Citrus aurantium is stir-fried Citrus aurantium; the fennel is salt-fried fennel; the Atractylodes macrocephala is stir-fried Atractylodes macrocephala; the lotus seed is lotus seed without heart; the Ophiopogon japonicus is Ophiopogon japonicus without heart; the Cyperus rotundus is stir-fried Cyperus rotundus; the Gardenia jasminoides is gardenia jasminoides stir-fried; the Pinellia ternata is processed Pinellia ternata; the Arisaema heterophyllum is processed Arisaema heterophyllum; the bitter almond is bitter almond with peel and tip removed; the Epimedium brevicornu is stir-fried Epimedium brevicornu; and the Sparganium stoloniferum is Sparganium stoloniferum processed with vinegar.

[0019] Preferably, the heating in step (2) above is steam heating, and the heating temperature is 75-85℃.

[0020] Preferably, the cooling temperature described in step (2) above is 25-30°C.

[0021] Preferably, the pressure of the compressed air in step (3) above is ≥0.40MPa.

[0022] Preferably, the settling time in step (3) above is more than 2 months. During the first 15 days of the settling process, compressed air needs to be introduced every day and the mixture needs to be stirred for 10-20 minutes.

[0023] Preferably, the step of separating the supernatant in step (3) above is to use a horizontal scraper centrifuge for separation, with a screen mesh of 80 mesh, a discharge speed of 4-6 r / min, a feed speed of 8-12 r / min, and a centrifugation speed of 40-44 r / min.

[0024] More preferably, the step of separating the supernatant in step (3) above is to use a horizontal scraper centrifuge for separation, with a screen mesh of 80 mesh, a discharge speed of 5 r / min, a feed speed of 10 r / min, and a centrifugation speed of 42 r / min.

[0025] Preferably, the step of separating the suspension in step (3) above is to use a horizontal screw discharge sedimentation turbine centrifuge for separation, with a drum speed of 2400-3000 r / min and a differential speed of 23-28 r / min.

[0026] Preferably, the step of separating the suspension in step (3) above is to use a horizontal screw discharge sedimentation turbine centrifuge for separation, with a drum speed of 2600 r / min and a differential speed of 25 r / min.

[0027] Preferably, the freezing temperature in step (4) above is ≤-3℃.

[0028] Preferably, the storage time described in step (4) above is 7-10 days.

[0029] Preferably, the filtration in step (4) above is carried out using a diatomaceous earth filter, with a pressure ≥0.1MPa after soil application and a final filtration pressure ≤0.35MPa.

[0030] Preferably, the medicinal wine contains the following components by weight: 60 parts Amomum villosum, 120 parts Cinnamomum cassia, 60 parts Amomum cardamomum, 30 parts Amomum villosum var. rubra, 60 parts Piper longum, 30 parts Aquilaria sinensis, 15 parts processed Polygonum multiflorum, 15 parts Rehmannia glutinosa, 15 parts Angelica dahurica, 15 parts Dioscorea opposita, 15 parts Galla chinensis, 15 parts Pogostemon cablin, 30 parts Ginseng, 15 parts Morus alba root bark, 15 parts Erythrina variegata bark, 15 parts Nardostachys jatamansi, 15 parts Angelica pubescens, 15 parts Atractylodes lancea, 15 parts Ligusticum chuanxiong, 15 parts Cuscuta chinensis, 15 parts Poria cocos, 15 parts Citrus reticulata peel, 15 parts Amomum tsao-ko, 15 parts Cornus officinalis, 15 parts Aconitum carmichaelii, 30 parts Magnolia officinalis, 15 parts Citrus reticulata peel, 15 parts Schisandra chinensis, 15 parts Achyranthes bidentata, 30 parts Citrus aurantium, and 30 parts Alpinia galanga. 15 parts of Kaempferia galanga, 15 parts of Tussilago farfara, 240 parts of Foeniculum vulgare, 60 parts of Platycodon grandiflorus, 30 parts of Rehmannia glutinosa, 30 parts of Acorus tatarinowii, 45 parts of Atractylodes macrocephala, 45 parts of Areca catechu, 30 parts of Glycyrrhiza uralensis, 90 parts of Angelica sinensis, 15 parts of Gentiana macrophylla, 60 parts of Carthamus tinctorius, 15 parts of Curcuma zedoaria, 15 parts of Nelumbo nucifera, 15 parts of Chaenomeles speciosa, 15 parts of Ophiopogon japonicus, 15 parts of Notopterygium incisum, 15 parts of Cyperus rotundus, 15 parts of Cistanche deserticola, 15 parts of Astragalus membranaceus, 15 parts of Asparagus cochinchinensis, 15 parts of Prunus persica, 15 parts of Gardenia jasminoides, 15 parts of Alisma plantago-aquatica, 15 parts of Lindera strychnifolia, 15 parts of Pinellia ternata, 15 parts of Arisaema heterophyllum, 15 parts of Prunus armeniaca, 30 parts of Poria cocos, 15 parts of Polygala tenuifolia, 15 parts of Epimedium brevicornu, 15 parts of Sparganium stoloniferum, 15 parts of Rubia cordifolia, 158,000 parts of Baijiu (Chinese white liquor), and 900 parts of red yeast rice.

[0031] The present invention also provides a medicinal wine prepared by the above method.

[0032] This invention also provides the application of the above-mentioned medicinal wine in the preparation of products for preventing cardiovascular and cerebrovascular diseases and improving microcirculation.

[0033] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0034] (1) This invention studies its preparation method. In the preparation process, the Chinese medicine components are extracted by batch crushing, mixed extraction, standing, freezing, and different separation methods to separate the supernatant and suspension. At the same time, through specific extracts and extraction methods, the effective components of the medicinal wine have high activity and good stability. It does not separate into layers or produce sediment after long-term storage.

[0035] This invention uses a horizontal scraper centrifuge to separate the supernatant. By controlling the screen mesh to 80 mesh, the discharge speed to 4-6 r / min, the feed speed to 8-12 r / min, and the centrifugation speed to 40-44 r / min, efficient and continuous solid-liquid separation is achieved.

[0036] This invention uses a horizontal screw discharge sedimentation turbine centrifuge to separate suspensions. With a drum rotation speed of 2400-3000 r / min and a differential speed of 23-28 r / min, solid-liquid separation can be better achieved, effectively improving the clarity of the liquid phase and reducing the water content of the solid phase.

[0037] In the implementation of this invention, the liquid medicine is frozen to a temperature of ≤-3℃ before filtration, which can effectively reduce the formation of precipitates in the liquid medicine and achieve efficient purification while retaining the effective ingredients.

[0038] The present invention achieves good stability in the medicinal wine through multiple centrifugation and filtration processes, and can maintain high clarity even after long-term storage.

[0039] (2) The medicinal wine prepared by the preparation method of the present invention has significant effects in preventing cardiovascular and cerebrovascular diseases and improving microcirculation. Detailed Implementation

[0040] The following non-limiting embodiments are intended to enable those skilled in the art to gain a more comprehensive understanding of the present invention, but do not limit the invention in any way. The following content is merely an exemplary description of the scope of protection claimed by the present invention, and those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, and such changes should also fall within the scope of protection claimed by the present invention.

[0041] When numerical ranges are given in the embodiments, it should be understood that, unless otherwise stated in the invention, both endpoints of each numerical range and any value between the two endpoints may be selected. Unless otherwise defined, all technical and scientific terms used in this invention have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0042] The present invention will be further described below by way of specific embodiments. Unless otherwise specified, all chemical reagents used in the embodiments of the present invention are obtained through conventional commercial means.

[0043] In the following examples, “part” and “g” have the same meaning, that is, 1 part = 1g.

[0044] Example 1: A method for preparing a medicinal wine

[0045] The medicinal wine contains the following components by weight: Amomum villosum 60g, Cinnamomum cassia 120g, Amomum cardamomum 60g, Amomum villosum 30g, Piper longum 60g, Agarwood 30g, Prepared Polygonum multiflorum 15g, Rehmannia glutinosa 15g, Angelica dahurica 15g, Dioscorea opposita 15g, Galla chinensis 15g, Pogostemon cablin 15g, Ginseng 30g, Morus alba root bark 15g, Erythrina variegata bark 15g, Nardostachys jatamansi 15g, Angelica pubescens 15g, Atractylodes lancea 15g, Ligusticum chuanxiong 15g, Cuscuta chinensis 15g, Poria cocos 15g, Citrus reticulata peel 15g, Amomum tsao-ko 15g, Cornus officinalis 15g, Aconitum carmichaelii 15g, Magnolia officinalis 30g, Citrus reticulata peel 15g, Schisandra chinensis 15g, Achyranthes bidentata 15g, Citrus aurantium 30g, Alpinia officinarum 30g, Kaempferia galanga 15g. g, Coltsfoot Flower 15g, Fennel 240g, Platycodon 60g, Rehmannia glutinosa 30g, Acorus tatarinowii 30g, Atractylodes macrocephala 45g, Areca catechu 45g, Licorice 30g, Angelica sinensis 90g, Gentiana macrophylla 15g, Carthamus tinctorius 60g, Curcuma zedoaria 15g, Nelumbo nucifera 15g, Chaenomeles speciosa 15g, Ophiopogon japonicus 15g, Notopterygium incisum 15g, Cyperus rotundus 15g, Cistanche deserticola 15g, Astragalus membranaceus 15g, Asparagus cochinchinensis 15g, Prunus persica 15g, Gardenia jasminoides 15g, Alisma plantago-aquatica 15g, Lindera strychnifolia 15g, Pinellia ternata 15g, Arisaema heterophyllum 15g, Prunus armeniaca 15g, Poria cocos 30g, Polygala tenuifolia 15g, Epimedium brevicornu 15g, Sparganium stoloniferum 15g, Rubia cordifolia 15g, Baijiu (Chinese white liquor) 158kg and Red yeast rice 0.9kg.

[0046] The preparation method is as follows:

[0047] (1) Crushing:

[0048] First, mix and grind the following ingredients: Amomum villosum, cinnamon, white cardamom, red cardamom, long pepper, and agarwood. Then add the following ingredients: prepared Polygonum multiflorum, Rehmannia glutinosa, Angelica dahurica, Dioscorea opposita, Galla chinensis, Pogostemon cablin, ginseng, Morus alba root bark, Erythrina variegata bark, Nardostachys chinensis, Angelica pubescens, Atractylodes lancea, Ligusticum chuanxiong, Cuscuta chinensis, Poria cocos, Citrus reticulata peel, Amomum tsao-ko, Cornus officinalis, Aconitum carmichaelii, Magnolia officinalis, Citrus reticulata peel, Schisandra chinensis, Achyranthes bidentata, Citrus aurantium, Alpinia officinarum, Kaempferia galanga, and Tussilago farfara. Fennel, Platycodon grandiflorus, Rehmannia glutinosa, Acorus tatarinowii, Atractylodes macrocephala, Areca catechu, Glycyrrhiza uralensis, Angelica sinensis, Gentiana macrophylla, Carthamus tinctorius, Curcuma zedoaria, Nelumbo nucifera, Chaenomeles speciosa, Ophiopogon japonicus, Notopterygium incisum, Cyperus rotundus, Cistanche deserticola, Astragalus membranaceus, Asparagus cochinchinensis, Prunus persica, Gardenia jasminoides, Alisma plantago-aquatica, Lindera strychnifolia, Pinellia ternata, Arisaema heterophyllum, Prunus armeniaca, Poria cocos, Polygala tenuifolia, Epimedium brevicornu, Sparganium stoloniferum, and Rubia cordifolia are mixed and pulverized to obtain a mixture with a particle size of 24-50 mesh.

[0049] (2) Extraction:

[0050] The mixture of baijiu (Chinese liquor), red yeast rice, and other ingredients is heated to 80°C and boiled, then cooled to 30°C to obtain a mixed liquid.

[0051] (3) Settling and separation:

[0052] The liquid medicine was sealed and allowed to stand for more than 2 months. During the first 15 days of the standing process, compressed air with a pressure of ≥0.40MPa was introduced daily and the mixture was stirred for 10 minutes. After the standing period, a horizontal scraper centrifuge was used for separation. The screen mesh was 80 mesh, the discharge speed was 4 r / min, the feed speed was 8 r / min, and the centrifugation speed was 40 r / min to separate the supernatant. A horizontal screw discharge sedimentation turbine centrifuge was used for separation. The drum speed was 2400 r / min and the differential speed was 23 r / min to separate the suspension and obtain the liquid medicine.

[0053] (4) Freezing and filtration:

[0054] The medicinal liquid is frozen to a temperature of ≤-3℃ and stored for 7 days. Then it is filtered using a diatomaceous earth filter. The pressure after applying the diatomaceous earth is ≥0.1MPa, and the final filtration pressure is ≤0.35MPa. If the pressure exceeds 0.35MPa, it needs to be cleaned and filtered again after applying the diatomaceous earth to obtain the medicinal wine.

[0055] Example 2: A method for preparing a medicinal wine

[0056] The medicinal wine contains the following components by weight: Amomum villosum 60g, Cinnamomum cassia 120g, Amomum cardamomum 60g, Amomum villosum 30g, Piper longum 60g, Agarwood 30g, Prepared Polygonum multiflorum 15g, Rehmannia glutinosa 15g, Angelica dahurica 15g, Dioscorea opposita 15g, Galla chinensis 15g, Pogostemon cablin 15g, Ginseng 30g, Morus alba root bark 15g, Erythrina variegata bark 15g, Nardostachys jatamansi 15g, Angelica pubescens 15g, Atractylodes lancea 15g, Ligusticum chuanxiong 15g, Cuscuta chinensis 15g, Poria cocos 15g, Citrus reticulata peel 15g, Amomum tsao-ko 15g, Cornus officinalis 15g, Aconitum carmichaelii 15g, Magnolia officinalis 30g, Citrus reticulata peel 15g, Schisandra chinensis 15g, Achyranthes bidentata 15g, Citrus aurantium 30g, Alpinia officinarum 30g, Kaempferia galanga 15g. g, Coltsfoot Flower 15g, Fennel 240g, Platycodon 60g, Rehmannia glutinosa 30g, Acorus tatarinowii 30g, Atractylodes macrocephala 45g, Areca catechu 45g, Licorice 30g, Angelica sinensis 90g, Gentiana macrophylla 15g, Carthamus tinctorius 60g, Curcuma zedoaria 15g, Nelumbo nucifera 15g, Chaenomeles speciosa 15g, Ophiopogon japonicus 15g, Notopterygium incisum 15g, Cyperus rotundus 15g, Cistanche deserticola 15g, Astragalus membranaceus 15g, Asparagus cochinchinensis 15g, Prunus persica 15g, Gardenia jasminoides 15g, Alisma plantago-aquatica 15g, Lindera strychnifolia 15g, Pinellia ternata 15g, Arisaema heterophyllum 15g, Prunus armeniaca 15g, Poria cocos 30g, Polygala tenuifolia 15g, Epimedium brevicornu 15g, Sparganium stoloniferum 15g, Rubia cordifolia 15g, Baijiu (Chinese white liquor) 158kg and Red yeast rice 0.9kg.

[0057] The preparation method is as follows:

[0058] (1) Crushing:

[0059] First, mix and grind the following ingredients: Amomum villosum, cinnamon, white cardamom, red cardamom, long pepper, and agarwood. Then add the following ingredients: prepared Polygonum multiflorum, Rehmannia glutinosa, Angelica dahurica, Dioscorea opposita, Galla chinensis, Pogostemon cablin, ginseng, Morus alba root bark, Erythrina variegata bark, Nardostachys chinensis, Angelica pubescens, Atractylodes lancea, Ligusticum chuanxiong, Cuscuta chinensis, Poria cocos, Citrus reticulata peel, Amomum tsao-ko, Cornus officinalis, Aconitum carmichaelii, Magnolia officinalis, Citrus reticulata peel, Schisandra chinensis, Achyranthes bidentata, Citrus aurantium, Alpinia officinarum, Kaempferia galanga, and Tussilago farfara. Fennel, Platycodon grandiflorus, Rehmannia glutinosa, Acorus tatarinowii, Atractylodes macrocephala, Areca catechu, Glycyrrhiza uralensis, Angelica sinensis, Gentiana macrophylla, Carthamus tinctorius, Curcuma zedoaria, Nelumbo nucifera, Chaenomeles speciosa, Ophiopogon japonicus, Notopterygium incisum, Cyperus rotundus, Cistanche deserticola, Astragalus membranaceus, Asparagus cochinchinensis, Prunus persica, Gardenia jasminoides, Alisma plantago-aquatica, Lindera strychnifolia, Pinellia ternata, Arisaema heterophyllum, Prunus armeniaca, Poria cocos, Polygala tenuifolia, Epimedium brevicornu, Sparganium stoloniferum, and Rubia cordifolia are mixed and pulverized to obtain a mixture with a particle size of 24-50 mesh.

[0060] (2) Extraction:

[0061] The mixture of baijiu (Chinese liquor), red yeast rice, and other ingredients is heated to 85°C and boiled, then cooled to 30°C to obtain a mixed liquid.

[0062] (3) Settling and separation:

[0063] The liquid medicine was sealed and allowed to stand for more than 2 months. During the first 15 days of the standing process, compressed air with a pressure of ≥0.40MPa was introduced daily and the mixture was stirred for 10 minutes. After the standing period, a horizontal scraper centrifuge was used for separation. The screen mesh was 80 mesh, the discharge speed was 6 r / min, the feed speed was 12 r / min, and the centrifugation speed was 44 r / min to separate the supernatant. A horizontal screw discharge sedimentation turbine centrifuge was used for separation. The drum speed was 3000 r / min and the differential speed was 28 r / min to separate the suspension and obtain the liquid medicine.

[0064] (4) Freezing and filtration:

[0065] The medicinal liquid is frozen to a temperature of ≤-3℃ and stored for 10 days. Then it is filtered using a diatomaceous earth filter. The pressure after applying the diatomaceous earth is ≥0.1MPa, and the final filtration pressure is ≤0.35MPa. If the pressure exceeds 0.35MPa, it needs to be cleaned and filtered again after applying the diatomaceous earth to obtain the medicinal wine.

[0066] Example 3: A method for preparing a medicinal wine

[0067] The medicinal wine contains the following components by weight: Amomum villosum 60g, Cinnamomum cassia 120g, Amomum cardamomum 60g, Amomum villosum 30g, Piper longum 60g, Agarwood 30g, Prepared Polygonum multiflorum 15g, Rehmannia glutinosa 15g, Angelica dahurica 15g, Dioscorea opposita 15g, Galla chinensis 15g, Pogostemon cablin 15g, Ginseng 30g, Morus alba root bark 15g, Erythrina variegata bark 15g, Nardostachys jatamansi 15g, Angelica pubescens 15g, Atractylodes lancea 15g, Ligusticum chuanxiong 15g, Cuscuta chinensis 15g, Poria cocos 15g, Citrus reticulata peel 15g, Amomum tsao-ko 15g, Cornus officinalis 15g, Aconitum carmichaelii 15g, Magnolia officinalis 30g, Citrus reticulata peel 15g, Schisandra chinensis 15g, Achyranthes bidentata 15g, Citrus aurantium 30g, Alpinia officinarum 30g, Kaempferia galanga 15g. g, Coltsfoot Flower 15g, Fennel 240g, Platycodon 60g, Rehmannia glutinosa 30g, Acorus tatarinowii 30g, Atractylodes macrocephala 45g, Areca catechu 45g, Licorice 30g, Angelica sinensis 90g, Gentiana macrophylla 15g, Carthamus tinctorius 60g, Curcuma zedoaria 15g, Nelumbo nucifera 15g, Chaenomeles speciosa 15g, Ophiopogon japonicus 15g, Notopterygium incisum 15g, Cyperus rotundus 15g, Cistanche deserticola 15g, Astragalus membranaceus 15g, Asparagus cochinchinensis 15g, Prunus persica 15g, Gardenia jasminoides 15g, Alisma plantago-aquatica 15g, Lindera strychnifolia 15g, Pinellia ternata 15g, Arisaema heterophyllum 15g, Prunus armeniaca 15g, Poria cocos 30g, Polygala tenuifolia 15g, Epimedium brevicornu 15g, Sparganium stoloniferum 15g, Rubia cordifolia 15g, Baijiu (Chinese white liquor) 158kg and Red yeast rice 0.9kg.

[0068] The preparation method is as follows:

[0069] (1) Crushing:

[0070] First, mix and grind the following ingredients: Amomum villosum, cinnamon, white cardamom, red cardamom, long pepper, and agarwood. Then add the following ingredients: prepared Polygonum multiflorum, Rehmannia glutinosa, Angelica dahurica, Dioscorea opposita, Galla chinensis, Pogostemon cablin, ginseng, Morus alba root bark, Erythrina variegata bark, Nardostachys chinensis, Angelica pubescens, Atractylodes lancea, Ligusticum chuanxiong, Cuscuta chinensis, Poria cocos, Citrus reticulata peel, Amomum tsao-ko, Cornus officinalis, Aconitum carmichaelii, Magnolia officinalis, Citrus reticulata peel, Schisandra chinensis, Achyranthes bidentata, Citrus aurantium, Alpinia officinarum, Kaempferia galanga, and Tussilago farfara. Fennel, Platycodon grandiflorus, Rehmannia glutinosa, Acorus tatarinowii, Atractylodes macrocephala, Areca catechu, Glycyrrhiza uralensis, Angelica sinensis, Gentiana macrophylla, Carthamus tinctorius, Curcuma zedoaria, Nelumbo nucifera, Chaenomeles speciosa, Ophiopogon japonicus, Notopterygium incisum, Cyperus rotundus, Cistanche deserticola, Astragalus membranaceus, Asparagus cochinchinensis, Prunus persica, Gardenia jasminoides, Alisma plantago-aquatica, Lindera strychnifolia, Pinellia ternata, Arisaema heterophyllum, Prunus armeniaca, Poria cocos, Polygala tenuifolia, Epimedium brevicornu, Sparganium stoloniferum, and Rubia cordifolia are mixed and pulverized to obtain a mixture with a particle size of 24-50 mesh.

[0071] (2) Extraction:

[0072] The mixture of baijiu (Chinese liquor), red yeast rice, and other ingredients is heated to 78°C and boiled, then cooled to 25°C to obtain a mixed liquid.

[0073] (3) Settling and separation:

[0074] The liquid medicine was sealed and allowed to stand for more than 2 months. During the first 15 days of the standing process, compressed air with a pressure of ≥0.40MPa was introduced daily and the mixture was stirred for 10 minutes. After the standing period, a horizontal scraper centrifuge was used for separation. The screen mesh was 80 mesh, the discharge speed was 5 r / min, the feed speed was 10 r / min, and the centrifugation speed was 42 r / min to separate the supernatant. A horizontal screw discharge sedimentation turbine centrifuge was used for separation. The drum speed was 2600 r / min and the differential speed was 25 r / min to separate the suspension and obtain the liquid medicine.

[0075] (4) Freezing and filtration:

[0076] The medicinal liquid is frozen to a temperature of ≤-3℃ and stored for 7 days. Then it is filtered using a diatomaceous earth filter. The pressure after applying the diatomaceous earth is ≥0.1MPa, and the final filtration pressure is ≤0.35MPa. If the pressure exceeds 0.35MPa, it needs to be cleaned and filtered again after applying the diatomaceous earth to obtain the medicinal wine.

[0077] Comparative Example 1: A method for preparing a medicinal wine

[0078] The product prepared by the method described in the examples of patent CN1192928A.

[0079] That is: Take Polygonum multiflorum, Rehmannia glutinosa, Angelica dahurica, Dioscorea opposita, Galla chinensis, Pogostemon cablin, Morus alba root bark, Erythrina variegata bark, Nardostachys chinensis, Angelica pubescens, Atractylodes lancea, Ligusticum chuanxiong, Cuscuta chinensis, Poria cocos, Citrus reticulata peel, Amomum tsao-ko, Cornus officinalis, Aconitum carmichaelii, Citrus reticulata peel, Schisandra chinensis, Achyranthes bidentata, Kaempferia galanga, Tussilago farfara, Gentiana macrophylla, Curcuma zedoaria, Nelumbo nucifera, Chaenomeles speciosa, Ophiopogon japonicus, Notopterygium incisum, Cyperus rotundus, Cistanche deserticola, Astragalus membranaceus, Asparagus cochinchinensis, Prunus persica, Gardenia jasminoides, Alisma plantago-aquatica, Lindera strychnifolia, Pinellia ternata, Arisaema heterophyllum, Prunus armeniaca, Polygala tenuifolia, Epimedium brevicornu, and three... 15g each of 44 herbs including Rhizoma Cyperi and Radix Rubiae; 30g each of 8 herbs including Radix Ginseng, Cortex Magnoliae Officinalis, Fructus Aurantii Immaturus, Radix Alpiniae Galangi, Radix Rehmanniae Praeparata, Rhizoma Acoruse Tatarinowii, Radix Glycyrrhizae, and Rhizoma Poriae; 45g each of Rhizoma Atractylodis Macrocephalae and Semen Arecae; 60g each of Radix Platycodi and Radix Carthami; 90g of Radix Angelicae Sinensis; 240g of Herba Eupatorii; 58 herbs in total are mixed and ground into powder. Then, 30g each of Fructus Amomi and Fructus Aquilariae; 60g each of Fructus Amomi, Fructus Amomi, and Fructus Piper Longi; and 120g of Fructus Cinnamomi; 6 herbs in total are mixed and ground into coarse powder.

[0080] Place 158 kg of baijiu (Chinese white liquor) and 0.9 kg of red yeast rice in a jar, then add the 64 pulverized Chinese medicinal herbs mentioned above. Cover the jar, heat it in a water bath until the liquor boils, pour it into a vat, cool it, seal it, and let it stand for more than two months. Take the supernatant, press the residue, clarify the extracted liquid, add 1 g of finely ground musk powder, stir well, seal and let it stand, combine it with the supernatant liquid, filter it, and you have the final product.

[0081] Comparative Example 2

[0082] The difference from Example 3 is that freezing was not performed; the centrifuged liquid was directly filtered. The other steps were the same as in Example 3, thus obtaining the medicinal wine.

[0083] Comparative Example 3

[0084] The difference from Example 3 is that after settling, only a horizontal scraper centrifuge is used for separation, with a screen mesh of 80 mesh, a discharge speed of 5 r / min, a feed speed of 10 r / min, and a centrifugation speed of 42 r / min to separate the mixture. The other operating steps are the same as in Example 3, thus obtaining the medicinal wine.

[0085] Comparative Example 4

[0086] The difference from Example 3 is that after settling, only a horizontal screw discharge sedimentation turbine centrifuge is used for separation. The drum speed is 2600 r / min and the differential speed is 25 r / min to separate the mixture. The other operating steps are the same as in Example 3, and the medicinal wine is obtained.

[0087] Comparative Example 5

[0088] The difference from Example 3 is as follows:

[0089] (3) Settling and separation:

[0090] The liquid medicine was sealed and allowed to stand for more than 2 months. During the first 15 days of the standing process, compressed air with a pressure of ≥0.40MPa was introduced daily and the mixture was stirred for 10 minutes. After the standing period, a horizontal scraper centrifuge was used for separation. The screen mesh was 80 mesh, the discharge speed was 8 r / min, the feed speed was 15 r / min, and the centrifugation speed was 50 r / min to separate the supernatant. A horizontal screw discharge sedimentation turbine centrifuge was used for separation. The drum speed was 2000 r / min and the differential speed was 20 r / min to separate the suspension and obtain the liquid medicine.

[0091] The other steps are the same as in Example 3, and the medicinal wine is obtained.

[0092] Effect Experiment

[0093] I. Stability Testing:

[0094] Experimental methods:

[0095] 1. Total solids content test:

[0096] Accurately measure 20 mL of the supernatant of the medicinal wine, place it in an evaporating dish dried to constant weight, evaporate it to dryness on a water bath, dry it at 105℃ for 3 hours, transfer it to a desiccator, cool it for 30 minutes, and quickly and accurately weigh it. A high and stable total solids content indicates that the content of the effective drug components in the medicinal wine is high and stable.

[0097] 2. Ethanol content: The content should be 36-38% according to the ethanol content determination method (General Rule 0711).

[0098] 3. The pH should be 4.5-5.5.

[0099] 4. Microbial limit test: The test shall be conducted in accordance with the Microbial Limit Test Method for Non-sterile Products in the Chinese Pharmacopoeia 2020 Edition.

[0100] 5. Identification: Thin-layer chromatography was used for detection.

[0101] The test results are shown in Table 1.

[0102] Table 1

[0103]

[0104] According to the test results in Table 1 above, the Hongmao prepared in Examples 1-3 of this invention has better properties, namely, a deep reddish-brown color, a slightly sweet and slightly bitter taste, and still maintains high clarity after 36 months of storage, with no precipitation and a high content of effective ingredients, all above 3.0g / 20mL. Comparative Example 1, prepared using conventional methods without centrifugation or filtration, resulted in significantly reduced stability; after 18 months of storage, a trace amount of precipitation occurred, and after 36 months, a precipitate easily dispersed by gentle shaking formed. Furthermore, the solid content, i.e., the content of effective ingredients, decreased, indicating significantly reduced stability. Comparative Example 2 did not undergo freezing before filtration. The failure to remove precipitated components during processing also affected the stability of the medicinal wine. After 36 months of storage, trace amounts of precipitate formed, and the solid content, i.e., the content of active ingredients, decreased. In Comparative Examples 3 and 4, using only one filtration method or changing the filtration parameters also affected the stability of the medicinal wine. In both examples, trace amounts of precipitate formed after 24 months of storage, and the solid content, i.e., the content of active ingredients, decreased. In Comparative Example 5, changing the operating parameters during centrifugation also affected the stability of the medicinal wine to some extent, causing trace amounts of precipitate to appear after 36 months of storage, and the solid content, i.e., the content of active ingredients, decreased.

[0105] The reason for the decreased stability of the medicinal wine is inferred to be:

[0106] Comparative Example 1 did not undergo oxygenation during the post-treatment standing process, resulting in a relatively lower solid content in the prepared medicinal wine compared to Examples 1-3 of this invention, meaning that the dissolution of the effective components was lower. In addition, no corresponding centrifugation and freezing treatments were performed during the post-treatment process, and a large number of macromolecules and poorly soluble components in the medicinal wine will settle and precipitate during long-term storage (18 months), meaning that the stability of the medicinal wine is not high.

[0107] In Comparative Example 2, since no freezing treatment was carried out during the post-treatment process, some poorly soluble components in the medicinal liquor settled and precipitated during long-term storage (36 months), that is, the stability of the medicinal liquor was not high.

[0108] In Comparative Example 3 and Comparative Example 4, only one centrifugation operation was used, and its effect of removing macromolecules and insoluble substances was not good, resulting in the settlement and precipitation of some poorly soluble components in the medicinal liquor during long-term storage (24 months), that is, the stability of the medicinal liquor was not high.

[0109] Although Comparative Example 5 used two centrifugation treatments, the operating parameters were not within the scope of protection of the present invention. Although it could remove macromolecules and insoluble substances to a certain extent, the effect was not good, resulting in the settlement and precipitation of some poorly soluble components in the medicinal liquor during long-term storage (36 months), that is, the stability of the medicinal liquor was not high.

[0110] The precipitation and settlement of solids during the storage of the medicinal liquor will lead to a decrease in the content of active ingredients in the medicinal liquor, thus affecting the efficacy of the medicinal liquor and customer satisfaction.

[0111] In summary, only the medicinal liquor prepared by the method provided by the present invention has better stability and no precipitation will occur during long-term storage.

[0112] II. Pharmacodynamic experiment

[0113] 1. Experimental purpose

[0114] Evaluate the promoting blood circulation and removing blood stasis effect of the medicinal liquor.

[0115] 2. Experimental materials

[0116] 2.1 Experimental animals

[0117] SD mice (SPF grade), half male and half female, body weight 200±20g; Kunming mice (SPF grade), body weight 20±2g, provided by the Guangdong Provincial Center for Medical Experimental Animals, animal quality certificate: SCXK (Guangdong) 2019-0002. The animals were raised in a SPF-grade barrier environment, with a temperature of 20-25°C and a relative humidity of 40-70%. After normal feeding for 3 days, they were used for the test. Experimental animal use license: SYXK (Guangdong) 2017-0125.

[0118] 2.2 Drugs and reagents

[0119] The medicinal liquor prepared in Example 3: Specification 500 mL / bottle (containing 7.3 g of crude drug).

[0120] Preparation of the drug: Take an appropriate amount of the medicinal liquor prepared in Example 3, rotate and evaporate it at 45°C until there is no alcohol smell, place it in a freeze dryer for freeze-drying, and prepare 85 mg of crude drug / g of freeze-dried powder for standby. When in use, it is reconfigured with distilled water to the required concentration for the test.

[0121] Dosage is based on:

[0122] The medicinal wine prepared in Example 3: For adults, the oral administration is twice daily, 15 ml each time. Assuming an adult weight of 60 kg, the average dosage is 7.3 mg crude drug / kg / day. The equivalent dose for mice is calculated as: 7.3 mg / kg / day × 12 = 87.6 mg crude drug / kg / day. In this experiment, the low, medium, and high doses for mice were designed to be 90, 180, and 360 mg crude drug / kg / day, respectively; the low, medium, and high doses for rats were designed to be 45, 90, and 180 mg crude drug / kg / day, respectively.

[0123] Aspirin tablets: Bayer Healthcare Co., Ltd., batch number: BJ41406; The daily clinical dose for adults is 50-300 mg. Since low doses of aspirin (75-150 mg) have antiplatelet effects, for an adult weighing 60 kg, the maximum antiplatelet dose is 150 mg / day, or 2.5 mg / kg / day. The equivalent dose for mice is: 2.5 mg / kg / day × 12 times = 30 mg / kg / day. The equivalent dose for rats is: 2.5 mg / kg / day × 6 times = 15 mg / kg / day.

[0124] Epinephrine hydrochloride: Wuhan Yuanda Pharmaceutical (China) Co., Ltd., batch number: 201902; Sodium chloride: Guangzhou Chemical Reagent Factory, batch number: 20191016; Potassium chloride: Guangzhou Chemical Reagent Factory, batch number: 20191118; Calcium chloride: Guangzhou Chemical Reagent Factory, batch number: 20191008; Sodium bicarbonate: Guangzhou Chemical Reagent Factory, batch number: 20191021; Glucose: Guangzhou Chemical Reagent Factory, batch number: 20200703-2.

[0125] 2.3 Experimental Apparatus

[0126] Blood rheology and viscosity analyzer (Zibo Hengtuo Analytical Instruments Co., Ltd., HT-100A); biological microscope (Macody Industrial Group Co., Ltd., BA310-T).

[0127] 3. Methods and Results

[0128] 3.1 Effects on the acute blood stasis model in rats

[0129] Sixty rats (half male and half female) were randomly divided into a blank control group, a model group, an aspirin positive drug group, and high, medium, and low dose medicinal wine groups. Each group was administered the corresponding drug via gavage at a volume of 10 ml / kg / day for 8 consecutive days. One hour after administration on the 8th day, except for the blank control group, all other groups received a subcutaneous injection of 0.8 ml / kg of 0.1% epinephrine hydrochloride injection twice, with a 4-hour interval between the two injections. Between the two injections (with a 2-hour interval between injections), the rats were placed in 0-2℃ ice water for 5 minutes to induce an acute blood stasis model. After successful modeling, the rats were fasted but allowed free access to water for 12 hours. One hour after the last administration, each group was anesthetized by intraperitoneal injection of sodium pentobarbital (30 mg / kg), and 4 ml of blood was collected from the abdominal aorta. The blood was placed in a pre-treated heparinized tube, gently shaken, and blood rheological parameters were measured using an automated hemorheometer within 4 hours. The results are shown in Tables 2 and 3 below.

[0130] Table 2. Effects of medicinal wine on plasma viscosity and whole blood viscosity in rats with acute blood stasis (n=10, mean ± standard deviation)

[0131]

[0132] Note: Compared with the blank control group, *P<0.05, **P<0.01; compared with the model group, # P < 0.05 ## P < 0.01.

[0133] As shown in Table 2, compared with the blank control group, the plasma viscosity and whole blood viscosity at different shear rates of the model group rats were significantly increased, indicating that the acute blood stasis model was successfully established. Compared with the model group, the plasma viscosity and whole blood viscosity at different shear rates of the aspirin positive drug group and the low, medium and high dose groups of medicinal wine were significantly decreased (P<0.05 or P<0.01), indicating that medicinal wine has the effect of reducing plasma viscosity and whole blood viscosity in rats with acute blood stasis.

[0134] Table 3. Effects of medicinal wine on erythrocyte deformability index, erythrocyte aggregation index, and hematocrit in rats with acute blood stasis (n=10, mean ± standard deviation).

[0135]

[0136] Note: Compared with the blank control group, *P<0.05, **P<0.01; compared with the model group, # P < 0.05 ## P < 0.01.

[0137] As shown in Table 3, compared with the blank control group, the hematocrit of erythrocytes in the model group rats was significantly increased (P<0.01); compared with the model group, the hematocrit of erythrocytes in the low, medium and high dose groups of the medicinal wine was significantly decreased (P<0.01 or P<0.05); the erythrocyte aggregation index of the high dose of medicinal wine was significantly decreased (P<0.01), indicating that the medicinal wine has the effect of improving erythrocyte aggregation in rats with acute blood stasis.

[0138] 3.2 Effect on coagulation time in mice

[0139] Seventy-five KM mice were randomly divided into a blank control group, an aspirin-positive control group, and high, medium, and low dose groups of medicated wine. Each group was administered the corresponding drug via gavage at a volume of 20 ml / kg for 8 consecutive days. One hour after the last administration, blood was collected from the inner canthus of the eye using a capillary glass tube. Timing began from the moment blood flowed into the tube. After the tube was full, the capillary was removed and placed flat on a table. Every 30 seconds, two capillaries were broken by approximately 0.5 cm and slowly pulled apart to the left and right, observing for any blood clots at the break points. The clotting time was defined as the time from the moment blood flowed into the tube until the appearance of blood clots. The results are shown in Table 4.

[0140] Table 4

[0141]

[0142] Note: Compared with the blank control group, *P<0.05, **P<0.01.

[0143] Compared with the blank control group, the aspirin positive drug group, as well as the low, medium, and high dose groups of the medicinal wine, all significantly prolonged the fractional clotting time.

[0144] 3.3 Effects on mesenteric microcirculatory disturbances in mice

[0145] Seventy-five KM mice were randomly divided into a blank control group, an aspirin-positive drug group, and high, medium, and low dose groups of medicated wine. The corresponding drugs were administered by gavage at a volume of 20 ml / kg for 8 consecutive days. Before the experiment, mice were anesthetized with 3% sodium pentobarbital (30 mg / kg) for 1 hour after the last administration. An incision was made in the midline of the abdomen, and the ileocecal junction was removed. The intestinal loops were placed in a glass trough containing Ringer-Tyr solution at 36.2℃. Vascular flow velocity and flow pattern were observed under a microscope before and after administration. The flow velocity and flow pattern parameters after adding adrenaline were recorded. Evaluation criteria are shown in Table 5, and results are shown in Table 6.

[0146] Table 5 Blood Flow Status Scoring Criteria

[0147]

[0148] Table 6. Effects of medicinal wine on mesenteric microcirculation disorders in mice (n=15, mean ± standard deviation)

[0149]

[0150] Note: Compared with the blank control group, *P<0.05, **P<0.01.

[0151] According to the results in Table 6 above, compared with the blank control group, the blood flow status scores of the aspirin positive drug group and the low, medium and high dose groups of the medicinal wine were significantly increased, indicating that the medicinal wine can significantly improve the blood flow velocity in the mesenteric microvessels of mice and has the effect of improving the mesenteric microcirculation disorder in mice.

[0152] The above embodiments are only used to illustrate the technical solutions of the present invention and not to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art can still make modifications or equivalent substitutions to the specific implementation schemes of the present invention, and these modifications or equivalent substitutions do not depart from the spirit and scope of the present invention, and are all within the protection scope of the claims of the present invention.

Claims

1. The application of a medicinal wine in the preparation of drugs for the prevention of cardiovascular and cerebrovascular diseases, characterized in that: The preparation method of the medicinal wine includes the following steps: (1) Crushing: First, mix and grind 60 parts of Amomum villosum, 120 parts of cinnamon, 60 parts of white cardamom, 30 parts of red cardamom, 60 parts of Piper longum, and 30 parts of agarwood. Then add 15 parts of processed Polygonum multiflorum, 15 parts of Rehmannia glutinosa, 15 parts of Angelica dahurica, 15 parts of Dioscorea opposita, 15 parts of Galla chinensis, 15 parts of Pogostemon cablin, 30 parts of ginseng, 15 parts of Morus alba root bark, 15 parts of Erythrina variegata bark, 15 parts of Nardostachys jatamansi, 15 parts of Angelica pubescens, 15 parts of Atractylodes lancea, 15 parts of Ligusticum chuanxiong, 15 parts of Cuscuta chinensis, 15 parts of Poria cocos, 15 parts of Citrus reticulata peel, 15 parts of Amomum tsao-ko, 15 parts of Cornus officinalis, 15 parts of Aconitum carmichaelii, 30 parts of Magnolia officinalis, 15 parts of Citrus reticulata peel, 15 parts of Schisandra chinensis, 15 parts of Achyranthes bidentata, 30 parts of Citrus aurantium, 30 parts of Alpinia galanga, and 15 parts of Kaempferia galanga. Coltsfoot flower 15 parts, fennel 240 parts, platycodon root 60 parts, prepared rehmannia root 30 parts, acorus root 30 parts, atractylodes rhizome 45 parts, areca nut 45 parts, licorice root 30 parts, angelica root 90 parts, gentian root 15 parts, safflower 60 parts, turmeric root 15 parts, lotus seed 15 parts, papaya 15 parts, ophiopogon root 15 parts, notopterygium root 15 parts, cyperus rhizome 15 parts, cistanche deserticola 15 parts, astragalus root 15 parts, asparagus root 15 parts, peach kernel 15 parts, gardenia fruit 15 parts, alisma rhizome 15 parts, lindera root 15 parts, pinellia tuber 15 parts, arisaema rhizome 15 parts, bitter almond 15 parts, poria cocos 30 parts, polygala root 15 parts, epimedium root 15 parts, sparganium rhizome 15 parts, and rubia root 15 parts are mixed and pulverized to obtain a mixture. (2) Extraction: The liquor, red yeast rice, and other ingredients are mixed, heated to a boil, and then cooled to obtain a mixture. (3) Settling and separation: Take the medicine solution, seal it and let it stand. During the standing process, compressed air needs to be introduced and stirred. After the standing is completed, separate the supernatant and the suspension to obtain the medicine solution. The step of separating the supernatant involves using a horizontal scraper centrifuge with an 80-mesh sieve, a discharge speed of 4-6 r / min, a feed speed of 8-12 r / min, and a centrifugation speed of 40-44 r / min. The step of separating the suspension involves using a horizontal screw discharge sedimentation turbine centrifuge with a drum speed of 2400-3000 r / min and a differential speed of 23-28 r / min. (4) Freezing and filtration: The medicinal liquid is frozen and stored, and then filtered to obtain the medicinal wine. The freezing temperature is ≤-3℃, and the storage time is 7-10 days.

2. The application according to claim 1, characterized in that: The particle size of the mixture in step (1) is 24-50 mesh.

3. The application according to claim 1, characterized in that: The heating in step (2) is steam heating, with a heating temperature of 75-85℃; the cooling temperature in step (2) is 25-30℃.

4. The application according to claim 1, characterized in that: The pressure of the compressed air in step (3) is ≥0.40MPa; the settling time is more than 2 months. During the first 15 days of the settling process, compressed air needs to be introduced every day and the mixture needs to be stirred for 10-20 minutes.

5. The application according to claim 1, characterized in that: The filtration described in step (4) is carried out using a diatomaceous earth filter, with a pressure ≥0.1MPa after the soil is applied and a final filtration pressure ≤0.35MPa.

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