Method for culturing anti-greensickness cotton by using artificially synthesized antimicrobial peptide gene

Abstract

The invention provides a method for culturing anti-greensickness cotton by using an artificially synthesized antimicrobial peptide gene. In the method, the artificially synthesized antimicrobial peptide gene is integrated into upland cotton cultivated species to realize the constitutive expression of the antimicrobial peptide gene spCEMA (Signal Peptide modified Cecropin A-Melittin) in genetically modified cotton so as to improve the disease resistance of the cotton to greensickness. In the invention, a gene CEMA with good disease-resistance effect is fused, the toxic and harmful effects to plant cells by in-vivo expression of a protein CEMA can be eliminated, and growth and development of the plant are not influenced while the disease resistance of the plant is improved. In the invention, by means of the constitutive expression of the artificially synthesized antimicrobial peptide gene spCEMA, the verticillium lecanii can be controlled within a certain range or can be inhibited in further growth in the plant, so that the purpose of resisting the greensickness is achieved and novel anti-greensickness cotton resources are cultured. By applying the method, the genetically modified anti-greensickness cotton with the disease index smaller than 10 or so can be obtained.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering. Specifically, it relates to a method for cultivating cotton resistant to Verticillium wilt by using an artificially synthesized antibacterial peptide gene. technical background [0002] Cotton is not only the most important natural fiber crop in the world, but also an important material related to the national economy and people's livelihood in my country. The cotton industry plays a pivotal role in the development of my country's textile industry and even the entire national economy. However, since cotton Verticillium wilt was introduced into my country in 1935, the harm to my country's cotton production has been increasing year by year. Verticillium wilt has become the main limiting factor for high and stable cotton production. The disease is a soil-borne vascular disease, which is characterized by wide distribution, severe damage, wide host range, multiple transmission rout...

AI Technical Summary

Problems solved by technology

However, after the plant-derived antimicrobial peptides are transferred to plants, pathogenic bacteria tend to develop resistance or have pathogenic specificity. Therefore, it is relatively difficult to obtain broad-spectrum and long-lasting resistance to Verticillium dahliae of different physiological races.

However, synthetic non-plant-derived antimicrobial proteins or antimicrobial peptides can overcome this deficiency of plant-derived antimicrobial peptides, but such antimicrobial proteins or antimicrobial peptides may seriously affect the growth and development of plants.

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