89results about How to "Does not affect growth and development" patented technology

The invention relates to a technology for preventing and controlling the growth of water sprouts of bananas by adopting chemical substances in proper quantity, which comprises the following operation methods that for banana water sprouts with different varieties and sizes, a sharp knife is used for cutting off the water sprouts to be flush with the ground, and a writing brush is used for smearing flumetralin liquid medicine on the cross sections of the water sprouts; or a hole puncher is used for downwards pressing the cross sections of the water sprouts from the middle parts to take out water sprout tissues to enable the middle parts of the water sprouts to form small holes, then an injector without a needle head is used for sucking flumetralin liquid medicine or diluent of the flumetralin liquid medicine, and the flumetralin liquid medicine in proper quantity is filled into the small holes of the water sprouts.

The invention discloses a degradable magnesium alloy esophageal stent for infants. The esophageal stent comprises annular single bodies, connecting points and projections, wherein each annular single body is formed in the way that a magnesium alloy metal wire is folded in the shape of W and connected to form a cylindrical shape; the annular single bodies have elasticity; bending parts are formed at the two ends of each annular single body; the annular single bodies are axially connected with one another in series by the connecting points; the bottom of each projection is positioned at each connecting point; the top end of each projection is positioned on the outer side of the esophageal stent; and after the magnesium alloy esophageal stent is expanded and released, the projections can be embedded into the esophageal wall and are used for fixing the stent. The degradable magnesium alloy esophageal stent for infants is formed by connecting a plurality of annular single bodies and the length of the esophageal stent can be determined by the number of the connected annular single bodies, so the requirements of the infants suffering from esophageal stenosis are met; the projections on the outer surface of the stent can be embedded into the inner wall of the esophagus and are used for fixing the stent to prevent the stent from moving or slipping off; and the stent can be degraded and absorbed by a human body and the degradation products have no toxicity, so growth and development of infantile patients are not influenced.

The invention provides application of a gene which can improve the agricultural production traits of rice and improve the bacterial blight disease resistance and includes application of rice miR156 and a target gene of rice miR156 in improving rice resistance to the bacterial leaf blight. The invention also provides a method for cultivating anti-bacterial leaf blight transgenic rice, which comprises the following steps of: constructing an over-expression vector containing OsSPL7 or OsSPL14, transforming the constructed over-expression vector into rice, and up-regulating the expression of riceOsSPL7 or IPA1/OsSPL14 to obtain the bacterial blight resistant transgenic rice; or down-regulating the expression of miR156 in rice crops so as to up-regulate miR156 target genes OsSPL7 and OsSPL14 (IPA1) to obtain the bacterial blight resistant transgenic rice. The method provided by the invention, on the condition of not affecting growth and development and improving the agronomic traits such as rice plant type and panicle type, takes microRNA as a starting point, provides a gene resource that affects rice plant type and panicle type, and also enhances resistance of rice to bacterial blight.

The present invention is one kind of disinfectant for disinfecting silkworm room, silkworm rearing implement, silkworm bed, silkworm and mulberry leaf. The disinfectant consists of main component concentrated bleaching powder, supplementary material anionic suitable surfactant sodium dodecyl benzene sulfonate and alkali assistant calcium hydroxide in the weight ratio of 25-50 to 1-4 to 1-2. It is mixed with water in 180-200 times to compound disinfecting liquid for disinfecting silkworm room, silkworm rearing implement and mulberry leaf; and mixed with lime powder in 60-80 for disinfecting silkworm and silkworm bed. The disinfecting liquid has high activity, high stability, broad germicidal spectrum, fast disinfecting effect and safety on silkworm.

The invention discloses a grass-growing cultivating mode of a macadamia integrifolia orchard. The grass-growing cultivating mode comprises the following steps of constructing an orchard and performingplanting; performing fertilizing management; performing water management; performing leader branch cutting and performing trimming; performing extermination of diseases and insect pests; and performing grass growing in the orchard. According to the grass-growing cultivating mode disclosed by the invention, in accordance with the characteristics that in China, macadamia integrifolia is mainly planted in mountainous regions and hilly regions, and the row spacing between plants is large, orchard grass growing management is performed on the orchards; and besides, cultivation management of soil, water and fertilizers and the like is optimized, a macadamia integrifolia cultivating mode which is high in per mu yield, and can effectively exterminate diseases and insect pests, and take account ofprevention of soil erosion is provided, and the cultivating mode has important significance on accelerating the development of the macadamia integrifolia, enlarging the planting area and improving incomes of peasants.

The invention belongs to the technical field of microbial fermentation, and provides a micro-ecological compound microbial agent and a method for processing a solid crop culture medium through the micro-ecological compound microbial agent for solving the problems that an existing culture medium cannot be reused, and if the culture medium is reused, continuous cropping obstacles are prone to being caused, and the culture medium becomes a carrier of soil-borne disease pollution and concentrated spread. The micro-ecological compound microbial agent is prepared by mixing bacillus amyloliquefaciens LH-1, bacillus licheniformis, pseudomonas cichorii, propionigenium modestum, thermotoga aritime, bacillus stearothermophilus, candida tropicalis, trichoderma harzianum, streptomycete, actinomyces meyeri and frankiaceae according to a certain ratio. According to the micro-ecological compound microbial agent and the method for processing the solid crop culture medium through the micro-ecological compound microbial agent, pathogenic bacteria can be effectively killed, and the continuous cropping obstacles are overcome; the mode that biological and physical measures are combined is scientifically applied to solve the problems existing in reuse of the matrix, the fruit and vegetable quality safety and zero pollution to the environment are guaranteed, the production cost of farmers is lowered, the agricultural product quality safety is guaranteed, and the ecological environment is protected; the culture medium can be reused for many times, and the growth and development of crops cannot be influenced.

The invention discloses a method for rapidly recovering paddy field pieces of wetland reverted by cultivated land into eastern meadow rush wetland, and belongs to the technical field of ecological engineering. The problems that in the natural recovery process of vegetation of paddy field pieces of wetland reverted by cultivated land, the recovery period is long and the stability is poor are solved. The method for rapidly recovering paddy field pieces of wetland reverted by cultivated land into eastern meadow rush wetland comprises the steps that 1, an eastern meadow rush transplanting body isdug, and the root system of eastern meadow rush carries original soil; 2, shallow reversing is performed on the paddy field pieces of wetland reverted by cultivated land; 3, the eastern meadow rush transplanting body is planted into a planting pit; 4, after transplanting, water is supplemented, and water is supplemented till the ground surface water depth reaches 1-3 cm; 5, after creep branch tipnewly-born plant bodies grow to 70-95 cm, creep branches are cut off, newly-born plant bodies of separated mother plant bodies grow and expand to be propagated, within one to three years, the newly-born plant body and mother plant body separating process is repeatedly performed, and natural growth and propagation of eastern meadow rush are kept. By adopting manual assistance for separation of creep branches and newly-born plant bodies, the newly-born plant body wild expanded propagation and multiplication capability is enhanced, and the recovery efficiency is improved.

The invention provides a method for culturing anti-greensickness cotton by using an artificially synthesized antimicrobial peptide gene. In the method, the artificially synthesized antimicrobial peptide gene is integrated into upland cotton cultivated species to realize the constitutive expression of the antimicrobial peptide gene spCEMA (Signal Peptide modified Cecropin A-Melittin) in genetically modified cotton so as to improve the disease resistance of the cotton to greensickness. In the invention, a gene CEMA with good disease-resistance effect is fused, the toxic and harmful effects to plant cells by in-vivo expression of a protein CEMA can be eliminated, and growth and development of the plant are not influenced while the disease resistance of the plant is improved. In the invention, by means of the constitutive expression of the artificially synthesized antimicrobial peptide gene spCEMA, the verticillium lecanii can be controlled within a certain range or can be inhibited in further growth in the plant, so that the purpose of resisting the greensickness is achieved and novel anti-greensickness cotton resources are cultured. By applying the method, the genetically modified anti-greensickness cotton with the disease index smaller than 10 or so can be obtained.

The invention provides a photoinduced promoter gene which has a nucleotide sequence or a homologous nucleotide sequence thereof. The photoinduced promoter gene comprises a RbcS gene from paddy Nipponbare of ssp.japonica of Oryza sativa (rice) and an intron sequence. The invention further provides a method of induced expression of exogenous gene in plants. The method includes: introducing a vector into a plant callus cell; growing to be a transgenic plant with photoinduced expression. The photoinduced promoter gene is utilized to replace a constitutive promoter to obtain a binary expression vector containing the photoinduced promoter; genetic transformation technology is utilized to guide the binary expression vector into a plant genome, and directional operation of a target gene can be realized to obtain a transgenic plant with photoinduced expression of the target gene.

The invention discloses a sewage infiltration purification treatment method. Sewage passing a grating is collected into a settling tank, flocculating agents are added and settled for 12-36 hours, thesewage is conveyed to an infiltration device, infiltrated and purified, the infiltration device comprises a primary infiltration tank and a secondary infiltration tank, the settling tank, the primaryinfiltration tank and a secondary infiltration tank are stepped, the sewage sequentially passes the settling tank, the primary infiltration tank and the secondary infiltration tank, and finally, effluent quality meets national discharge standards. A constructed wetland technology, a biological treatment technology and a multilayer infiltration technology are effectively and organically combined and integrated, suspended matters, total phosphorus, total nitrogen and ammonia nitrogen in the sewage can be effectively removed, and the method is low in energy consumption, small in occupied area, low in operation cost, convenient in management and maintenance, low in equipment counterfeiting cost and wide in applicable place and applicable landform range and has a good application prospect.

The invention discloses a composite biological preparation for treating domestic sludge, a preparation method and a treatment method thereof. The composite biological preparation comprises: alcaligenes, arthrobacter, and stenotrophomonas maltophilia; wherein the weight ratio of alcaligenes, arthrobacter, and stenotrophomonas maltophilia is 22-26: 15-20:10-14. The method combines specific strains together, and can effectively decompose organic substances in the sludge after being treated for a certain period of time under normal temperature conditions, and stabilizes the sludge after standing for a certain period of time, which can effectively remove ammonia and H2S from the sludge, removes the foul odor in the sludge, and releases most of the moisture in the sludge in a short time, the treated sludge can be used as a soil amendment, an organic fertilizer, and the like, can be directly used for planting crops without affecting the growth and development of crops, and does not affect thequality of crops.

The invention relates to a rice and shrimp co-culture planting-breeding method. The rice and shrimp co-culture planting-breeding method comprises the following steps: 1) rice field transformation; 2)water body disinfection; 3) young shrimp stocking; 4) Bacillus subtilis pouring; 5) rice cultivation; 6) rice and shrimp management; and 7) rice and shrimp harvesting. According to the invention, through pouring of the Bacillus subtilis into a water body in an annular gully, the Bacillus subtilis has strong inhibition effect on harmful microorganisms like vibrios, colibacillus and baculovirus in aquatic products, so the occurrence of diseases and pests of shrimps can be effectively prevented; the Bacillus subtilis also can consume harmful substances like ammonia nitrogen and nitrogen nitrite in a water body, so the water body is purified, and the dissolved oxygen content of the water body is improved; and by utilization of a water-flooding method, pesticide-free prevention and treatment ofdiseases and pests of rice are realized, so no influence on growth and development of the shrimps is caused, and the yield and quality of the rice and the shrimps are improved. The method provided bythe invention realizes zero use of a chemical fertilizer and a pesticide; and an agricultural product produced by using the method provided by the invention is healthy, reliable, ecological and environmentally-friendly.

The invention discloses a stevia rebaudiana seed harvesting device and method. The device comprises a machine head, a collection box and a harvesting machine; the machine head buckles the collection box, and the harvesting device is communicated with the collection box; the machine head comprises a shell, a motor, a centrifugal fan and an air filter; the motor, the centrifugal fan and the air filter are located in the shell and arranged in sequence from top to bottom, and the motor is in transmission connection with the centrifugal fan and drives the centrifugal fan; an export port and an air-suction inlet are formed in the top and the bottom of the shell respectively, and a filter is arranged at the air-suction inlet; the harvesting machine comprises a suction nozzle, a handle and a hose;one end of the handle is connected with the suction nozzle, and the other end of the handle is communicated with the collection box through the hose. Accordingly, when mature seeds of stevia rebaudiana are harvested, the loss of seeds is less, the harvested seeds are mature seeds, and then the yield of the seeds is increased, and the quality of the seeds is improved; meanwhile, manpower and material resources are saved in the harvesting process, and the production efficiency is improved.

The invention provides a plant expression vector for a vascular peculiar promoter to control two types of antimicrobial protein gene and a method for cultivating greensickness-resistant cotton. In the method, motherwort antimicrobial protein genes LjAMP2 and LjAMP2 controlled by a vascular tissue peculiar promoter AAP2 are integrated into a cotton genome to realize the high-amount expression of the antimicrobial protein gene in the vascular tissue of the transgenic cotton root and properly express in the vascular tissues of other parts of the plant so as to obviously improve the resistance to greensickness by cotton. After performing homozygosis to defoliating and nondefoliating greensickness bacteria inoculated by a T2 generation plant greenhouse disease pool, the transgenic cotton obtained with the method compares the defoliating and nondefoliating greensickness bacteria with Non-GMO, and disease index can be respectively lowered by 40% and 80%.

A silkworm breeding feeding mulberry leaf pretreatment method comprises the following steps: cleaning picked mulberry leaves with 5% salt water once, and drying in air; and soaking the dried mulberry leaves in 50ppm sodium hypochlorite solution for 10min, and soaking the mulberry leaves in sodium dichloro isocyanurate solution; according to the method, the 5% salt water, sodium hypochlorite and the sodium dichloro isocyanurate are used for three layer disinfection of the mulberry leaves, the concentration of effective ingredients can be strictly controlled, the processing time can be reasonably arranged, various harmful parasitic bacteria in the mulberry leaves can be effectively killed, mulberry leaf itself nutrition composition is not destroyed, and the incidence of the disease of silkworm breeding can be reduced.

The invention relates to a mesh-bag-transplanted seedling breeding method for maritime pine seedlings, which comprises the steps that (1) soil of a seedling breeding region selected for use is disinfected; (2) following air and water permeable mesh bags are selected as seedling breeding containers, wherein the mesh bag comprises a mesh bag body and a mesh bag bottom connected with the mesh bag body, and the areas of meshes on the mesh bag body and the mesh bag bottom are 0.25 to 1 square centimeters; (3) the seedlings are lifted; (4) the seedlings are bagged; (5) the seedlings are colonized; and (6) the seedlings are managed. The method has the advantages that through using the mesh bags woven by nylon and provided with the meshes of appropriate sizes as the transplanting containers, the maritime pine seedlings throughout grow in the mesh bags in the processes from seedling breeding to transplantation and afforestation, processes of transfer and packing are avoided, working procedures of the afforestation are reduced, the efficiency is improved, the seedling transplanting survival rate is increased, and the process of the afforestation becomes simpler, and is more convenient and easier to realize.

The invention provides an application of protein in regulating and controlling the selenium content of plant tissues and/or organs. The protein is any one of the following proteins: a) protein with anamino acid sequence as shown in SEQ ID No.7; b) fusion protein obtained by connecting a label to an N terminal or/and C terminal of the protein as shown in SEQ ID No.7; and c) protein related to selenium metabolism and obtained by substituting and/or deleting and/or adding one or more amino acid residues to the amino acid sequence as shown in SEQ ID No.7. The selenium content of the plant tissuesand/or organs is regulated and controlled to increase the selenium content of the plant tissues and/or organs. The selenium content in the plant tissues and/or organs is regulated and controlled to increase the selenium content in the plant tissues and/or organs. The organs are seeds; and plants are spermatophytes.

The invention aims to liberate manual labor and provides a paddy rice herbicide, which is composed of potassium hydroxide, p-nitrochlorobenzene, phenol, chlorine, and sodium sulfite. The paddy rice herbicide provided by the invention can kill weeds in the weed germination period, has small phytotoxicity to paddy rice, and does not affect the growth and development of paddy rice.

The invention relates to the field of agricultural planting and soil improvement agents, in particular to a special green and environment-friendly type acid soil improvement agent for facility agriculture. The special green and environment-friendly type acid soil improvement agent is characterized in that the agricultural wastes, namely soybean straws, are used as the main component and carrier ofthe acid soil improvement agent, a plurality of natural plant bacteriocidins and insecticides are screened, the formula composition is optimized and proportioned, and finally the special green and environment-friendly type acid soil improvement agent prepared from soybean straws, radix asparagi officinalis, tobacco, herba taraxaci, folium cacumen biotae, shell powder, nanometer chitosan and compound trace elements is obtained. The special green and environment-friendly type acid soil improvement agent has the advantages that the cost is low, the operation is convenient, the safety in use is realized, the stability is realized, the effect is lasting, the acid soil structure of the facility can be obviously improved, and the problem of soil-borne pests and diseases due to continuous cropping of soil is solved; a novel path is provided for the circulation of soybean straws, the natural resource is saved, the energy crisis is relieved, and the ecological environment is protected.

The invention relates to a cold-proof overwintering method for grape vines. The method comprises the following steps: fixing the grape vines onto a fixing device which is away from the ground by the height of 40-50 cm before freezing-up of soil; covering the soil with non-woven fabrics in the planting row direction; and compacting two ends of each non-woven fabric with soil, wherein the non-woven fabrics are filament non-woven fabrics, and the specification of the non-woven fabrics is 500-700 g/m<2>. The method can have windproof, moisturizing, insulating and cold-proof effects effectively, and meanwhile has effects of simplicity in operation, capability of saving time and labor, cost reduction, and reduction of damage to branches, eyes and root systems of the grape vines.

The invention discloses a broiler wing number. The broiler wing number comprises three parts of a serial number end, a penetrating end and a cap end; one side of the serial number end is connected with the penetrating end, the serial number end and the penetrating end are integral structure which is beneficial for that the serial number end is not prone to being lost, the cap end is a hollow pipewith one end being open, an inserting plate is arranged on the side, close to the penetrating end, of the interior of the cap end, and the inserting plate can allow the penetrating end to be insertedirreversibly. The broiler wing number has the advantages that 1, the structure is simple, and the broiler wing number is irreversible after being worn, and can be served as an anti-fake label; 2, thedefect that a traditional broiler wing number is prone to falling down is avoided when the broiler wing number is worn, and the growth and development of chicken are not affected; 3, manpower is savedby wearing the broiler wing number than wearing a traditional one, and the working efficiency is high; and 4, the production cost is low.

The invention provides a method for cultivating verticillium wilt-resistant cotton by utilizing gene synergism, and application thereof. The method utilizes transgenic technology to transfer a chitinase gene Bbchit1 and an antibacterial protein gene LjAMP2 resistant to verticillium wilt into cotton respectively, integrates two disease-resistant genes with different sources into the same plant through sexual hybridization, realizes simultaneous constitutive expression of different disease-resistant genes in the same plant, and utilizes the synergism of the two genes to effectively improve the disease resistance of the cotton to verticillium wilt. Results show that disease index is between 10 and 25 after transgenic cotton obtained by the method is inoculated with defoliating-type verticillium wilt pathogens in an artificial climate chamber and can be reduced by over 75 percent compared with that of wild-type cotton. The method is simple and easy to implement, significant in effects, capable of breeding verticillium wilt-resistant cotton and particularly providing novel resistance resources for defoliating-type verticillium wilt-resistant breeding, brings huge economic benefit to cotton production, and has good market prospects.

The invention relates to insect population trajectory prediction and pest control effect evaluation equipment based on laser radar and a layout method, and belongs to the technical field of agriculture and forestry pest monitoring and evaluation. The equipment comprises a moving device, a monitoring system and a computer terminal control system, wherein the moving device comprises a track and a loading vehicle, the loading vehicle is arranged on the track, and the monitoring system is arranged on the loading vehicle; the monitoring system comprises a camera, a laser radar, a carrying rod and a telescopic rod, wherein the telescopic rod is installed on the carrying vehicle through a rotating base, the carrying rod is arranged at the top end of the telescopic rod, the camera and the laser radar are arranged on the two sides of the carrying rod respectively, and the camera, the laser radar, the telescopic rod, the rotating base and the carrying vehicle are all connected to the computer terminal control system. The maneuverability and stability of the device are improved by using the surrounding type motion track, the monitoring range is wider, the monitoring dead angle is reduced, the flying direction and the motion track of insect groups can be automatically monitored and forecasted, and the pest control effect is evaluated.