Sparklet testing method of soya-bean fatty acid

A soybean fatty acid and detection method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to detect trace amounts of components, cannot realize absolute content detection, deviation between measured value and real value, etc., and achieves important practical significance Effect

Inactive Publication Date: 2008-06-25
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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Problems solved by technology

However, most studies have only carried out the determination of the relative content of fatty acids, and cannot realize the detection of their absolute content.
Although some studies can carry out quantitative detection of fatty acids, most of them use external standard method, which makes there is a certain deviation between the measured value and the real value, and requires a large amount of samples, which cannot effectively carry out trace detection of the content of each component

Method used

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  • Sparklet testing method of soya-bean fatty acid

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Embodiment Construction

[0015] The invention is applied in the subject of "Broadening and Improving Soybean Functional Nutrient Resources" of the Northeast Institute of Geography and Agricultural Ecology, Chinese Academy of Sciences. During the implementation of the subject, the present invention was used to analyze and evaluate more than 200 soybean resources in Northeast China, and obtained two soybean materials with high linoleic acid content and two soybean materials with low linolenic acid content. Breeding has played an effective technical support. The test results of fatty acid content in some soybean resource seeds are listed below:

[0016] Detection results of fatty acid content in some soybean resource grains (μg / 100g)

[0017]

[0018] According to the test results, it can be seen that there is a large genetic variation in the content of fatty acid components in soybean resources in Northeast China. In the relative content of each component, linoleic acid>oleic acid>palmitic acid>lin...

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Abstract

The invention belongs to the agricultural production technical field and relates to a micro detection method for soybean fatty acid content. Based on the prior detection method for soybean fatty acid, the invention realizes extraction of total fatty acid contained in soybean grain through adopting half-grain method to complete sampling, taking hexane as internal standard substance and combining with triglyceride; then, methanol esterification of methanol solution in which potassium hydroxide is added is completed in water bath; finally, when centrifugation of the fatty acid mixture obtained after methanol esterification is completed, the supernatant is taken out for precise quantitative detection in gas phase chromatogram. The invention has important practical significance in soybean high quality oil content breeding and improvement.

Description

technical field [0001] The invention belongs to the technical field of agricultural production. It is a trace detection method of soybean fatty acid content. technical background [0002] Soybean oil contains essential fatty acids, which have important nutritional and health value. The quality of soybean oil depends on the composition and ratio of main fatty acids. Linoleic acid and linolenic acid, the unsaturated fatty acids in soybean, are important components of biofilm, and their content is an important measure of oil quality, while linolenic acid is easy to oxidize, causing soybean oil to deteriorate, reducing the usability and performance of soybean oil. Reduce shelf life. Therefore, from the perspective of nutrition and storage, it is very important to increase the content of oleic acid and linoleic acid in unsaturated fatty acids, appropriately reduce the content of palmitic acid and linolenic acid, and make the proportion of fatty acid components reasonable. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 潘相文李艳华王国栋邢海军智小明梁桂荣兰志华
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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