Method for cultivating verticillium wilt-resistant cotton by utilizing gene synergism, and application thereof

Abstract

The invention provides a method for cultivating verticillium wilt-resistant cotton by utilizing gene synergism, and application thereof. The method utilizes transgenic technology to transfer a chitinase gene Bbchit1 and an antibacterial protein gene LjAMP2 resistant to verticillium wilt into cotton respectively, integrates two disease-resistant genes with different sources into the same plant through sexual hybridization, realizes simultaneous constitutive expression of different disease-resistant genes in the same plant, and utilizes the synergism of the two genes to effectively improve the disease resistance of the cotton to verticillium wilt. Results show that disease index is between 10 and 25 after transgenic cotton obtained by the method is inoculated with defoliating-type verticillium wilt pathogens in an artificial climate chamber and can be reduced by over 75 percent compared with that of wild-type cotton. The method is simple and easy to implement, significant in effects, capable of breeding verticillium wilt-resistant cotton and particularly providing novel resistance resources for defoliating-type verticillium wilt-resistant breeding, brings huge economic benefit to cotton production, and has good market prospects.

Description

technical field The invention belongs to the field of plant genetic engineering. The invention mainly involves a method for cultivating transgenic cotton resistant to Verticillium wilt by using the synergy between genes from different sources. technical background Crop diseases are one of the main obstacles to high and stable crop yields, which often cause the quality of crop products to decline, and even produce some metabolites that are toxic to humans. The annual crop yield reduction caused by diseases in the world exceeds 15%, and the direct economic loss reaches 30-50 billion US dollars (Li Runzhi et al., 2001; Osusky et al., 2000). Breeding and popularizing disease-resistant varieties is the most economical and effective measure for integrated crop disease control. Due to the rapid mutation of pathogenic bacteria, limited plant resistance resources and the time-consuming and labor-intensive conventional breeding methods, the cultivated disease-resistant varieties are...

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Problems solved by technology

However, studies in this area have mainly focused on the synergistic effect between chitinase gene and glucanase gene (Wu Jiahe and Zhang Xianlong et al., 2004), but there is no improvement in the synergistic effect between chitinase gene and antibacterial protein gene. Report on Resistance of Transgenic Cotton to Verticillium Wilt

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