Photoinduced promoter gene and application thereof

Abstract

The invention provides a photoinduced promoter gene which has a nucleotide sequence or a homologous nucleotide sequence thereof. The photoinduced promoter gene comprises a RbcS gene from paddy Nipponbare of ssp.japonica of Oryza sativa (rice) and an intron sequence. The invention further provides a method of induced expression of exogenous gene in plants. The method includes: introducing a vector into a plant callus cell; growing to be a transgenic plant with photoinduced expression. The photoinduced promoter gene is utilized to replace a constitutive promoter to obtain a binary expression vector containing the photoinduced promoter; genetic transformation technology is utilized to guide the binary expression vector into a plant genome, and directional operation of a target gene can be realized to obtain a transgenic plant with photoinduced expression of the target gene.

Description

technical field

[0001] The invention belongs to the field of genetic engineering, and in particular relates to a light-inducible promoter gene and its application. Background technique

[0002] A promoter is an integral part of a gene that is recognized by RNA polymerase and starts transcription. Constitutive promoters are widely used in plant transgenics, such as CaMV 35S promoter, ubiquitin promoter and actin promoter, which are commonly used in transgenic Bt gene insect-resistant rice. Constitutive expression of the source protein in transgenic plants can also bring about many negative effects. For example, it increases the metabolic burden of plants, affects the growth and development of plants, causes plant growth retardation, short plants, and reduced yield; in addition, the use of constitutive promoters may induce gene silencing, which affects the application of transgenic technology; With the growing concern of plant food safety issues, constitutive promoters canno...

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