A kind of Enteromorpha microbial bacterial agent and preparation method thereof
A technology for microbial inoculants and prolifera, applied in the field of microbial inoculants and fertilizers, can solve problems such as inability to comprehensively apply, and achieve the effects of improving physical and chemical properties, strengthening root systems, and improving the quality of agricultural products
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Embodiment 1
[0044] Enteromorpha microbial bacterial agent described in the present embodiment is prepared by the following steps from the following components:
[0045] S1, taking fresh Enteromorpha and subjecting it to high-temperature sterilization above 100°C, sterilizing, soaking, crushing, and grinding to make Enteromorpha slurry to obtain Enteromorpha slurry;
[0046] S2, respectively insert the preserved Streptomyces flavinus, Lactobacillus casei, Bacillus subtilis, Rhodopseudomonas palustris and Pichia membranoidis into their respective suitable culture medium for aseptic culture, and perform aseptic culture according to the Streptomyces flavus, Lactobacillus casei, Bacillus subtilis, Rhodopseudomonas palustris and Pichia membranoides were mixed in a weight ratio of 2:3:1:3:1 to obtain biological strains ;in,
[0047] The formula of the Streptomyces luteus culture solution is: soluble starch 2.0g, potassium nitrate 0.1g, dipotassium hydrogen phosphate 0.05g, sodium chloride 0.05g...
Embodiment 2
[0056] Enteromorpha microbial bacterial agent described in the present embodiment is prepared by the following steps from the following components:
[0057] S1, taking fresh Enteromorpha and subjecting it to high-temperature sterilization above 100°C, sterilizing, soaking, crushing, and grinding to make Enteromorpha slurry to obtain Enteromorpha slurry;
[0058] S2, respectively insert the preserved Streptomyces flavinus, Lactobacillus casei, Bacillus subtilis, Rhodopseudomonas palustris and Pichia membranoidis into their respective suitable culture medium for aseptic culture, and perform aseptic culture according to the Streptomyces flavus, Lactobacillus casei, Bacillus subtilis, Rhodopseudomonas palustris and Pichia membranoides were mixed in a weight ratio of 4:1:3:1:3 to obtain biological strains ; Wherein, the culture medium that each strain uses is all identical with embodiment 1;
[0059] S3, get the enteromorpha slurry 35kg that step S1 obtains, the biological strain ...
Embodiment 3
[0063] Enteromorpha microbial bacterial agent described in the present embodiment is prepared by the following steps from the following components:
[0064] S1, taking fresh Enteromorpha and subjecting it to high-temperature sterilization above 100°C, sterilizing, soaking, crushing, and grinding to make Enteromorpha slurry to obtain Enteromorpha slurry;
[0065] S2, respectively insert the preserved Streptomyces flavinus, Lactobacillus casei, Bacillus subtilis, Rhodopseudomonas palustris and Pichia membranoidis into their respective suitable culture medium for aseptic culture, and perform aseptic culture according to the Streptomyces flavus, Lactobacillus casei, Bacillus subtilis, Rhodopseudomonas palustris and Pichia membranosa in a weight ratio of 3:1.5:2:2:1.5 and mixed evenly to obtain biological strains ; Wherein, the culture medium that each strain uses is all identical with embodiment 1;
[0066] S3, get the enteromorpha slurry 32kg that step S1 obtains, the biological...
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