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Method for preparing xylitol by fermenting xylose secondary mother liquor

A technology of xylitol and xylose hydrolyzate, which is applied in the field of xylitol production by secondary fermentation of xylose mother liquor, can solve the problems of complex fermentation operation steps, low conversion rate of xylitol, high production cost, etc., and avoid conversion The efficiency is low, it is convenient for subsequent purification, and the effect of saving dosage

Inactive Publication Date: 2021-06-25
ZHEJIANG HUAKANG PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a method for preparing xylitol by fermenting the secondary mother liquor of xylose, by mixing the secondary mother liquor of xylose and the concentrated xylose liquor for fermentation according to a certain ratio, and at the same time increasing the initial glucose content of the material In order to reduce the amount of subsequent glucose feed, and replace the subsequent glucose feed with the xylose secondary mother liquor, solve the problems of low xylose content in the xylose secondary mother liquor, low xylitol conversion rate, complicated fermentation operation steps, and high production costs problems, to achieve the purpose of increasing the added value of the xylose secondary mother liquor

Method used

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  • Method for preparing xylitol by fermenting xylose secondary mother liquor
  • Method for preparing xylitol by fermenting xylose secondary mother liquor
  • Method for preparing xylitol by fermenting xylose secondary mother liquor

Examples

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Effect test

Embodiment 1

[0045] A kind of first embodiment of the method that utilizes xylose secondary mother liquor fermentation to prepare xylitol of the present invention, comprises the steps:

[0046] Step 11, preparation of xylose concentrate: add 0.4% Ca(OH) to the xylose hydrolyzate before separation 2 , stirring at 50 rpm for 15 min, controlling the pH to 3.2, then adding activated carbon with a mass ratio of 0.4% for decolorization, controlling the temperature at 60° C., and stirring at 60 rpm for 90 min. After the decolorization is completed, the filtered xylose liquid is concentrated in a low-temperature vacuum, and the vacuum pump pressure is set at -0.095MPa, the water bath temperature is 60°C, the rotation speed is 60rpm, and the flow rate of the condensed water is about 200mL / min until the refraction of the concentrated solution is about 65%. Xylose concentrate suitable for fermentation.

[0047] Step 12, strain activation and preparation of seed solution: select recombinant Escherich...

Embodiment 2

[0054] A kind of second embodiment of the method that utilizes xylose secondary mother liquor fermentation to prepare xylitol of the present invention comprises the steps:

[0055] Step 21, preparation of xylose concentrate: add 0.5% Ca(OH) to the xylose hydrolyzate before separation 2 , stirred at 30 rpm for 30 min, controlled pH 3.0, then added 0.5% activated carbon by mass for decolorization, controlled temperature at 70°C, and stirred at 30 rpm for 60 min. After the decolorization is completed, the filtered xylose liquid is concentrated in a low-temperature vacuum, and the vacuum pump pressure is set at -0.085MPa, the water bath temperature is 70°C, the rotation speed is 50rpm, and the condensed water flow rate is about 150mL / min until the refraction of the concentrated solution is about 60%. Xylose concentrate suitable for fermentation.

[0056] Step 22, strain activation and preparation of seed solution: select the recombinant Escherichia coli strain IS5-M, activate the...

Embodiment 3

[0063] A kind of third embodiment of the method that utilizes xylose secondary mother liquor fermentation to prepare xylitol of the present invention comprises the steps:

[0064] Step 31, preparation of xylose concentrate: add 0.4% Ca(OH) to the xylose hydrolyzate before crossing 2 , stirring at 50 rpm for 15 min, controlling the pH to 3.5, then adding activated carbon with a mass ratio of 0.3% for decolorization, controlling the temperature at 60° C., and stirring at 60 rpm for 90 min. After the decolorization is completed, the filtered xylose liquid is concentrated in a low-temperature vacuum, and the vacuum pump pressure is set at -0.095MPa, the water bath temperature is 60°C, the rotation speed is 60rpm, and the flow rate of the condensed water is about 200mL / min until the refraction of the concentrated solution is about 65%. Xylose concentrate suitable for fermentation.

[0065] Step 32, strain activation and seed solution preparation: select recombinant Escherichia col...

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Abstract

The invention relates to a method for preparing xylitol by fermenting xylose secondary mother liquor, the method comprises the following steps: fermenting mixed liquor of the xylose secondary mother liquor and xylose concentrated liquor, and replacing subsequent glucose fed-batch with the xylose secondary mother liquor in the fermentation process, so that the stable glucose content in a fermentation system is met, and part of xylose can be provided, the glucose consumption can be saved, the cost is reduced, the relative stability of the system xylose can be maintained, and the overall conversion rate is improved. With applicaiton of the xylose secondary mother liquor added in the modes of mixed fermentation, fed-batch and the like, the content of miscellaneous sugars such as arabinose and mannose in a fermentation system is always within the metabolizable range of fermentation strains, subsequent purification is facilitated, and the purity of xylitol can be improved.

Description

technical field [0001] The invention relates to the technical field of sugar alcohol preparation, in particular to a method for preparing xylitol by secondary mother liquid fermentation of xylose. Background technique [0002] In the process of preparing xylose, corncobs and other raw materials are obtained through processes such as acid hydrolysis, decolorization, separation, concentration, crystallization, centrifugation and drying to obtain crystalline xylose; during the centrifugation process, a large amount of xylose mother liquor will be obtained, which can be chromatographically The xylose is separated and extracted again, and the raffinate is obtained at the same time during the chromatographic separation process, which is the secondary mother liquor of xylose. The secondary mother liquor of xylose contains multiple monosaccharides such as glucose, arabinose, galactose, mannose and xylose, and the content fluctuates due to different production conditions and batches,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/38C12P7/18C12R1/19
CPCC12N1/20C12N1/38C12P7/18Y02E50/10C12R2001/19C12N1/205C12N2500/14C12N2500/32C12N2500/34C12N2500/74C12N2500/76
Inventor 曾徐浩罗家星王静胡昌辉方顺成柳志强蔡雪张晓健郑裕国李勉
Owner ZHEJIANG HUAKANG PHARMA
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