35 results about "Macadamia integrifolia" patented technology

The invention provides an anti-premature senility management method for closely-planted double-layer truncated cone macadamia nut trees, and belongs to the technical field of fruit tree cultivation. The premature senility prevention management method comprises layered nutrition supply and flower and fruit protection management. The layered nutrition supply comprises the following steps: deeply applying a calcium magnesium phosphate fertilizer to the lower layer of soil greater than 60cm, and covering a root controller; organic fertilizers and biological agents are applied to 30-60 cm soil of the middle layer; a macroelement, humic acid and seaweed extract water-soluble fertilizer is applied to the 0-30 cm soil of the upper layer; the flower and fruit protection management comprises the steps that ABA and paclobutrazol are sprayed in the flower bud differentiation period; brassinolide and fluid boron are sprayed in the full-bloom stage; spraying forchlorfenuron and amino acid in a young fruit stage; nAA and monopotassium phosphate are sprayed in the fruit swelling period. Layered nutrition supply and flower and fruit protection management are carried out on the densely-planted double-layer truncated cone macadamia nut trees, premature senility of the densely-planted macadamia nut trees is prevented, branch development of the lower layer is promoted, the tree vigor is balanced, and the high-yield level is maintained.

The invention provides a method for screening drought-enduring germplasm resources of macadamia nuts, and belongs to the technical field of plant germplasm resource screening. The screening method comprises the following steps: (1) sowing disinfected macadimia nut seeds into a seedling raising tray, and raising seedlings to obtain seedlings; (2) transplanting the seedlings obtained in the step (1) into a nutrient medium, and performing drought stress culture to obtain pretreated seedlings; and (3) determining drought tolerance indexes in the pretreated seedlings obtained in the step (2), and screening to obtain the macadimia nut drought-tolerant germplasm resources. According to the screening method provided by the invention, macadamia nut germplasm resources with relatively strong drought tolerance can be rapidly screened out, and powerful support is provided for cultivation of drought-tolerant varieties.

The invention discloses a molecular marker primer group for identifying a macadamia nut variety Yunnan No.10, a kit and application. The molecular marker primer group is composed of three pairs of primers, and each pair of primers is composed of an upstream primer and a downstream primer. An upstream primer of the first pair of primers is as shown in SEQ ID NO: 1 in a sequence table, a downstream primer of the first pair of primers is as shown in SEQ ID NO: 2 in the sequence table, an upstream primer of the second pair of primers is as shown in SEQ ID NO: 3 in the sequence table, a downstream primer of the second pair of primers is as shown in SEQ ID NO: 4 in the sequence table, an upstream primer of the third pair of primers is as shown in SEQ ID NO: 5 in the sequence table, and a downstream primer of the fourth pair of primers is as shown in SEQ ID NO: 6 in the sequence table. The downstream primer of the third pair of primers is shown as SEQ ID NO: 6 in the sequence table. The molecular marker primer group can accurately identify the macadimia nut variety Yunnan No.10.

The cultivation method comprises the steps that the macadamia nut forest with the tree age larger than or equal to 5 years is selected, PVC permeable pipes with water seepage holes are laid at the bottoms of ditches, and the ditches are covered with gravel layers; ridging is conducted along the dead furrows, and a lower layer of fallen leaf crushed matter mixed decomposed farmyard manure, a middle layer of acid red soil and an upper layer of special konjak matrix containing functional microorganisms are constructed on ridge bodies in a layered mode; performing cold shock treatment on the seed taros, soaking the seed taros in chitosan-nano-selenium composite liquid, and coating the seed taros with nut oleoresin; the height of the sunshade net is dynamically regulated and controlled through an intelligent system to maintain the shading rate to be 60%-70%, and automatic drip irrigation is carried out based on a soil humidity sensor; harvesting and retaining rhizomes after konjak seedlings fall, removing konjak plants before nut fruit drop, and collecting dropped fruits by utilizing dead furrows. According to the method, the problems of water and light accurate regulation and control, root competition and harvesting conflicts are solved, waste recycling is achieved, the soft rot rate is decreased, the fruit drop loss rate is greatly decreased, and the comprehensive output value is remarkably increased.

The invention provides a planting method for interplanting rhizoma bletillae in a macadamia nut garden, and belongs to the field of macadamia nut planting. Compared with macadamia nut trees without interplanting the rhizoma bletillae, the method for interplanting the rhizoma bletillae and the macadamia nut trees in the garden has the advantages that the growth amount of the macadamia nut trees is increased, and the growth amount difference in the aspects of tree height, diameter thickness and crown breadth is obvious because the macadamia nut trees absorb nutrition and moisture while the rhizoma bletillae is fertilized and watered, so that the yield of the macadamia nut trees is increased, and the yield of the macadamia nut trees is increased. They belong to a mutual promotion relationship. Meanwhile, the macadamia nuts and the rhizoma bletillae are interplanted, so that the output value per mu is increased.

The invention discloses a management method for improving cold resistance and drought resistance of macadamia nuts and increasing flowering quantity, which combines a four-stage dynamic management mode of soil improvement, cold resistance induction, drought resistance and tip control and flowering phase quality improvement, applies an organic fertilizer, a water-retaining agent and a composite slow-release fertilizer, induces root development, slowly releases the fertility of a chemical fertilizer, prolongs the action time of the chemical fertilizer, and improves the yield of the macadamia nuts. Brassinolide and potassium fulvate are compounded to activate cold-resistant gene expression of macadamia nut plants and improve cell membrane stability, so that cold resistance of the macadamia nut plants is improved, melatonin and methyl jasmonate perform double shoot control, an ABA signal channel of the plants is triggered, drought resistance of the plants is improved, flower bud differentiation is promoted, and growth of the macadamia nut plants is promoted. The synergistic effect of alginate oligosaccharide and gamma-aminobutyric acid enhances the elongation ability of a pollen tube, chemical agent stimulation is replaced by biological agent stimulation, the utilization rate of chemical agents is reduced, then the negative influence of the chemical agents on soil is reduced, and finally the fruit setting rate and the sustainable utilization of the soil are improved.

The invention provides a method for planting potherb mustard under a dry season macadamia nut forest to improve planting benefits, and belongs to the technical field of agriculture. According to the method, the double-cropping potherb mustard is planted in the dry season and under the macadamia nut forest, firstly, the soil humidity of the macadamia nut garden is improved, the soil fertility is enhanced, the flowering quality of the macadamia nuts is improved, and the fruit setting rate and the yield of the macadamia nuts are increased; 2, under-forest space resources are fully utilized, and under-forest planting earnings are increased. By means of the method, the productivity, economic benefits and ecological benefits of the macadamia nut orchard are improved; and the method has very strong popularization and application prospects.

The utility model relates to the technical field of nut planting, in particular to a planting device for macadamia nut planting, which is characterized in that inner plates are arranged on the inner sides of all bearing pipes, top plates are fixedly connected to the top ends of all the inner plates, and supporting plates are rotatably connected to all the top plates through pin shafts; the tops of all the supporting plates are fixedly connected with arc-shaped supporting plates, the bottom of the arc-shaped base is fixedly connected with a plurality of fixing rods, the problem that an existing planting device lacks an effective fixing and limiting mechanism in the planting process is effectively solved, the straightness degree of saplings is ensured, and the subsequent growth quality and yield of the saplings are improved.

The present invention discloses a cultivation and management method for reducing the incidence rate of polyembryonic fruits in macadamia nuts, which comprises the following steps: S1. Starting from the flower bud differentiation period of the macadamia nut fruit tree until before the withering of flowers, spray with a growth-promoting hormone every 18 - 25 days to promote the normal development of floral organs such as stamens and pistils; and during the flower bud differentiation period from November to December, if there is a drought, irrigate appropriately to keep the soil moisture content not less than 65%; S2. In February - March every year, during the flowering period of the macadamia nut fruit tree, spray with the following fertilizers every 12 - 25 days: 500 - 1500 times solution of Yuhualing No. 6, 0.01 - 0.3% potassium dihydrogen phosphate, 0.01 - 0.3% amino acid foliar fertilizer, 0.005 - 0.2% boron fertilizer. The method of the present invention can effectively reduce the incidence rate of polyembryonic fruits in macadamia nuts.

This invention discloses a method for simultaneous flowering and fruiting of macadamia nuts. First, in September of the current year, pre-harvest fertilizer is applied to the 695 macadamia nut variety before fruit harvest. In October, when the fruit matures, 1 / 5 to 1 / 3 is harvested, with the remaining fruit left on the branches. Post-harvest pruning is carried out in October and November, leaving branches that did not bear fruit unpruned; harvested branches are lightly pruned. From November to March of the following year, the remaining fruit is harvested in batches, leaving no more than 1 / 4 of the fruit, with branch pruning performed simultaneously during harvest. Before flower bud differentiation in November, a flower-promoting fertilizer is sprayed to encourage flower bud differentiation. Around March of the following year, the 695 macadamia nut variety flowers, achieving simultaneous flowering and fruiting, with all remaining fruit harvested. This invention addresses the low natural fruit drop rate of the 695 macadamia nut variety by harvesting a portion of the fruit during the October ripening period, leaving the rest on the branches, and then harvesting in batches according to market conditions to stagger market entry. Simultaneously, the fruit is used to suppress the sprouting of autumn / winter and spring shoots, promote flower bud differentiation, and reduce the workload of post-harvest pruning.

The invention belongs to the technical field of planting, and discloses a hormone and leaf surface boron fertilizer spraying method for improving the fruit setting rate of macadamia ternifolia, and the method comprises the following steps: spraying 0.02% boron fertilizer in the flower bud period of macadamia ternifolia; 2 mg / L 6-BA is sprayed in the full-bloom stage of the macadamia nuts; 0.02% of boron fertilizer and 0.2 ml / L of brassinolide are sprayed from the flowering period of the macadamia nuts to the initial fruit setting period. The 0.02% boron fertilizer is sprayed in the flower bud period of the macadamia nuts, so that the pollen viability and the flower quantity of the macadamia nuts are increased, and the fruit setting probability and the total fruit setting quantity in the later period are increased. According to the method, 2 mg / L 6-BA is sprayed at the full-bloom stage of the macadamia nuts, so that the flower falling rate is reduced, and the fruit setting rate is increased.

The invention provides a method for reducing the dead fruit rate of macadamia nuts and preventing pericarp yellowing, which comprises the following steps: pruning fruit trees in the early stage of flower bud differentiation, spraying a composite insecticide in the early stage of flowering, and keeping the soil moisture content to be 60% or above. Spraying fruit retention liquid in a flower thinning and fruit retention period, using a root conditioning fertilizer and an insecticide in a flower falling and fruit setting period, spraying a leaf fertilizer and an insecticide in a fruit expanding period, and applying potassium sulfate in a final shell hardening and grease conversion period. By adding the anti-stiff fruit root slow-release fertilizer into the root conditioning fertilizer, it is guaranteed that fruit trees can continuously absorb mineral elements in high-temperature weather to reduce the stiff fruit rate, foliar fertilizer is sprayed to improve cell antioxidant enzyme activity and form a protective film, and the yellowing probability is reduced, so that the passing rate and peel yellowing of the fruit trees in the growth cycle are reduced, and the fruit quality is improved. The economic benefits of the macadamia nuts are greatly improved.

This invention discloses a method for eliminating moss growth on macadamia nut leaves, relating to the field of agricultural planting technology. The specific method involves spraying a moss-removing agent onto the macadamia nut leaves during the moss occurrence period, applying moss inhibitor I to the main stem, and applying moss inhibitor II to the soil surface around the main stem. The moss-removing agent comprises the following raw materials in parts by weight: 40-50 parts of allicin, 15-24 parts of urea, 5-18 parts of calcium propionate, 20-27 parts of calcium sulfate, 10-17 parts of calcium chloride, 20-35 parts of vegetable oil, 1-10 parts of titanium dioxide, 1-5 parts of glutathione, and 1-3 parts of dispersant. This invention solves the problems of slow effectiveness, short duration of action, and low plant safety in current macadamia nut leaf moss control methods, achieving highly efficient control.

The invention discloses a macadamia nut cutting seedling raising method. The method comprises the following steps: S1, selecting a lignified branch which grows for 1-2 years and has a diameter of 0.5-1.0 cm on a macadamia nut stock plant; s2, cutting slips are sheared from branches, and leaf, top and base treatment is carried out; s3, performing section disinfection on the cutting slips; s4, the bases of the cutting slips are placed in rooting liquid medicine to be quickly dipped for 5-10 s; s5, using 800-fold liquid of thiophanate-methyl to soak leaves on the tops of the cutting slips; s6, cutting the cutting slips in a seedbed with a river sand matrix; s7, keeping the seedbed and cutting slip leaves wet; s8, removing new ear tips on the cutting slips within 3 months after cuttage in time; s9, after 2-3 rounds of leaves of new tips of the cutting slips are matured, the cutting slips are dug out and transplanted into the seedling raising bag; s10, uncovering the sunshade net after 4-5 rounds of leaves of the new tips of the cutting slips are matured; s11, after 10-15 days, the seedling raising bags are moved out of the nursery garden. The method is simple to operate and easy to master and implement, the cuttage survival rate of the macadamia nuts reaches 90% or above, and the cuttage seedling rate of the macadamia nuts is remarkably increased.

The application provides a method for preventing early decline of densely-planted double-layered circular table-shaped macadamia nut trees, and belongs to the technical field of fruit tree cultivation. The method for preventing early decline comprises hierarchical nutrient supply and flower and fruit protection management. The hierarchical nutrient supply comprises: deep application of calcium, magnesium and phosphorus fertilizer in the lower layer of more than 60cm soil, and covering with a root control device; application of organic fertilizer and biological bacteria agent in the middle layer of 30-60cm soil; and application of a large amount of elements, humic acid and seaweed extract water-soluble fertilizer in the upper layer of 0-30cm soil. The flower and fruit protection management comprises: spraying ABA and paclobutrazol during flower bud differentiation period; spraying brassinolide and fluid boron during full flowering period; spraying forchlorfenuron and amino acid during young fruit period; and spraying NAA and potassium dihydrogen phosphate during fruit swelling period. The application supplies nutrients hierarchically and protects flowers and fruits for densely-planted double-layered circular table-shaped macadamia nut trees, prevents early decline of the densely-planted macadamia nut trees, promotes the development of lower branches, balances tree vigor and maintains high yield.

The invention discloses a molecular marker primer group and a kit for identifying a macadamia nut variety Guijin No.1 and application of the molecular marker primer group and the kit. The molecular marker primer group is composed of five pairs of primers, and each pair of primers is composed of an upstream primer and a downstream primer. An upstream primer of the first pair of primers is as shown in SEQ ID NO: 1 in a sequence table, a downstream primer of the first pair of primers is as shown in SEQ ID NO: 2 in the sequence table, an upstream primer of the second pair of primers is as shown in SEQ ID NO: 3 in the sequence table, a downstream primer of the second pair of primers is as shown in SEQ ID NO: 4 in the sequence table, an upstream primer of the third pair of primers is as shown in SEQ ID NO: 5 in the sequence table, and a downstream primer of the fourth pair of primers is as shown in SEQ ID NO: 6 in the sequence table. The downstream primer of the third pair of primers is shown as SEQ ID NO: 6 in the sequence table, the upstream primer of the fourth pair of primers is shown as SEQ ID NO: 7 in the sequence table, and the downstream primer of the fourth pair of primers is shown as SEQ ID NO: 8 in the sequence table. The molecular marker primer group can accurately identify the macadamia nut variety Guire No.1.

The application discloses a method for undergrowth near-natural interplanting of black chicken nest fungus in a macadamia nut forest, which comprises the following steps: (1) fungus bed digging and sterilization treatment: removing undergrowth dry branches, fallen leaves and sundries on the surface of soil of the macadamia nut forest, then digging a fungus bed in the macadamia nut forest, and performing sterilization treatment on soil of the fungus bed; (2) fungus bag making: taking a macadamia nut shell, sundry wood chips, corn cobs, wheat bran, corn flour, soybean meal and lime as raw materials to make a cultivation substrate, sterilizing the cultivation substrate after bagging, then inoculating black chicken nest fungus spores into the cultivation substrate and sealing, so as to obtain the fungus bag. The method scientifically plans the distribution position of the fungus bed, utilizes the macadamia nut shell to make the cultivation substrate, realizes efficient utilization of resources, improves the yield of the macadamia nut, and can obtain the black chicken nest fungus with high yield, so that the win-win of ecology and economic benefits is promoted.

The invention provides a macadimia nut leaf structure characteristic database, a variety identification and breeding method and application, and belongs to the technical field of plant variety identification and breeding. According to the method, leaf structure features are utilized, and a macadamia leaf structure feature database is constructed by collecting leaf samples and measuring multiple core indexes such as leaf length, the number of stomata and the number of veins; the method is simple, the operation is simple and convenient, and complex equipment is not needed. Moreover, the method is high in accuracy, the leaf structure characteristics are subjected to genetic control, the environmental interference is small, and the identification accuracy can reach 92% or above. The macadimia nut leaf structure feature database constructed by the method provides an efficient solution for variety breeding, the breeding efficiency is improved by 40%, and the breeding period is shortened by 2-3 years. In addition, the technical achievement of the invention can be directly applied to seedling breeding, variety right protection and breeding practice, and has a wide industrialization prospect.

The invention discloses a macadamia nut three-dimensional planting system based on an AMF fungicide and a drought resisting method thereof.The AMF fungicide comprises glomus mosseae, arbuscular mycorrhiza, phosphate solubilizing bacteria, nitrogen-fixing bacteria, humus and biochar, the AMF fungicide is used for planting macadamia nuts, the three-dimensional planting system of the macadamia nuts and under-forest crops is combined, and the drought resisting capability of the macadamia nuts is improved. And the drought resistance method of soil improvement, precise irrigation and foliage spraying of the drought-resistant agent can improve the drought resistance of the macadamia nuts. The macadamia nut three-dimensional planting system based on the AMF fungicide and the drought resisting method of the macadamia nut three-dimensional planting system are applied to tropical monsoon climate and subtropical low-latitude mountain monsoon climate areas, and the survival rate and yield of macadamia nuts can be increased.

The invention relates to the field of intelligent agriculture, and particularly discloses a macadamia nut disease detection and recognition method based on image recognition, which comprises the following steps: S100: image data acquisition: acquiring high-definition digital images of leaves and fruits at the middle and upper parts of a macadamia nut tree canopy from multiple angles under a natural light condition, the method comprises the following steps: collecting an object covering healthy leaves and disease samples which are confirmed as anthracnose, leaf blight and stem canker on site by agricultural experts, and accurately marking each collected image to generate a data set containing image file names and corresponding disease category labels, wherein the disease samples comprise different disease severity degrees, leaf growth stages and background environments; s200, performing image input processing, and taking the data set obtained in S100 as input data; s300, image processing is carried out; s400, obtaining a final recognition result; according to the technical scheme, disease features can be learned in a self-adaptive mode, complex environment interference is resisted, and recognition precision and efficiency are both considered.

The present invention provides a protein for promoting early flowering and altering fatty acid composition, its coding gene and applications, belonging to the field of biotechnology, and specifically discloses the application of the gene of Macadamia integrifolia in promoting early flowering and altering fatty acid composition. By studying the gene of the NF-YB family in Macadamia integrifolia, the present invention helps to understand the flowering and fatty acid synthesis mechanisms of Macadamia integrifolia, and uses genetic engineering techniques and the like to induce plants to flower early and alter fatty acid composition, thereby providing a theoretical basis and reference for the research on the mechanisms of regulating flowering and fatty acid synthesis accumulation. DRAP1 and its coding gene and applications, belonging to the field of biotechnology, and specifically discloses the application of the gene of Macadamia integrifolia in promoting early flowering and altering fatty acid composition. DRAP1 By studying the gene of the NF-YB family in Macadamia integrifolia, the present invention helps to understand the flowering and fatty acid synthesis mechanisms of Macadamia integrifolia, DRAP1 and uses genetic engineering techniques and the like to induce plants to flower early and alter fatty acid composition, thereby providing a theoretical basis and reference for the research on the mechanisms of regulating flowering and fatty acid synthesis accumulation.

The present invention provides a method for rapidly determining the open pollination intensity of macadamia nuts and its application, belonging to the field of cultivation techniques. The present invention provides a method for rapidly determining the open pollination intensity of macadamia nuts. At the early flowering stage, pistils with slightly swollen ovaries are sampled, fixed with FAA and then sectioned. The sections are observed and the percentage of pistils PLS with pollen tubes growing to the base of the style and the ovary is counted. The open pollination intensity of macadamia nuts is evaluated according to the value of PLS. From the start of sample collection to the completion of pollen tube fluorescence microscopy detection, the present invention only takes 30 h to know the open pollination intensity of all tested varieties. It has a small workload, intuitive, accurate and reliable results, and little damage. It can quickly detect the insect pollination efficiency in the current year for macadamia nut plantations, provide a basis for whether to carry out artificial supplementary pollination in the current year and reasonably place beehives, and can be widely used in macadamia nut plantations on a large scale.

The invention provides a salt-tolerant bacillus YH30 and application thereof, and relates to the technical field of microbial control. The salt-tolerant bacillus YH30 is classified and named as the salt-tolerant bacillus, the salt-tolerant bacillus YH30 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on December 2, 2024, and the preservation number is CGMCC No: 32877; the salt-tolerant bacillus YH30 is applied to prevention and treatment of grapefruit penicilliosis, blueberry fruit gray mold and macadamia nut anthracnose. The method overcomes the defects in the prior art, can effectively control the harm of grapefruit fruit penicilliosis, blueberry fruit gray mold and macadimia nut leaf anthracnose, and promotes the improvement of the resistance level of grapefruit fruits, blueberry fruits and macadimia nut leaves; the method has a positive promotion effect on the sustainable and healthy development of the planting industry of grapefruits, blueberries and macadamia nuts.

The application discloses a kind of methods for reducing the incidence of high temperature yellowing and disease spot of macadamia, comprising the following steps: natural grass between the rows of macadamia trees, or interplanting short economic crops;During the period of May to June, each tree is applied with a fertilizer comprising potassium fulvate and potassium sulfate;During the period of June to September, alternately spray fungicide and promote 1-2 times of shoot liquid;Carry out suitable water management.The application combines the use of improving soil environment, preventing the growth of pathogenic bacteria, promoting branch and shoot maturity, etc., to protect the tender shoots and new leaves during the summer shoot sprouting period, reduce the occurrence of high temperature yellowing, disease spot and leaf fall, etc., of the fruit trees, thereby better ensuring the normal growth of the fruit trees and the normal development of the fruits.

This invention discloses a method for near-natural intercropping of bamboo fungus under macadamia nut trees. The method includes the following steps: (1) preparing a cultivation substrate, then composting a portion of the cultivation substrate into a mature substrate, and using the other portion as raw substrate; (2) clearing away fallen leaves and debris under the macadamia nut trees, leveling the understory land and drying it, then creating a fungal bed in the tree basin of each macadamia nut tree, and digging drainage ditches around each fungal bed; the flat upper surface of the fungal bed is the raised bed surface; (3) soaking the raw substrate from step (1) in water and spreading it on the raised bed surface to form a raw substrate layer; then using the mature substrate from step (1) to lay a mature substrate layer on top of the raw substrate layer. When using the method provided in this invention to cultivate bamboo fungus, the yield of bamboo fungus is relatively stable, and the recycling of agricultural waste is realized, improving economic and ecological benefits.

This invention discloses an effective method for controlling leaf curling in macadamia nuts, comprising the following steps: selecting mineral oil with an effective ingredient content ≥99%, mixing it with water at a volume ratio of 1:100-400, then adding an emulsifier accounting for 0.05%-0.2% of the total weight of the mixture, stirring evenly to prepare a mineral oil emulsion; applying the emulsion using a misting device based on the growth characteristics of macadamia nuts during the shoot emergence period, pre-flowering period, young fruit stage, and mid-fruit development stage, combined with the differences in insect resistance among different varieties; integrating four measures: pruning and shaping control, orchard sanitation, soil regulation, and nutrient enhancement; and regularly monitoring key indicators such as thrips population density, leaf curling rate, and deformed fruit rate using a five-point sampling method, adjusting the control plan based on quantitative data. This invention achieves highly efficient control of thrips, prevents leaf curling, improves macadamia nut yield and quality, and protects the orchard ecological environment by optimizing the mineral oil emulsion formulation, precise application strategies, synergistic agricultural management measures, and dynamic monitoring and adjustment.

This invention discloses a method for identifying anthracnose resistance in macadamia nuts, belonging to the field of macadamia nut resistance breeding technology. The method includes steps such as: isolation of the anthracnose pathogen, preparation of mycelial cakes, inoculation of healthy macadamia nut leaves by scrambling, disease index classification, and evaluation of resistance and susceptibility characteristics. This application involves culturing macadamia anthracnose to obtain purified anthracnose mycelia, then inoculating healthy leaves by scrambling; classifying the disease index by the diameter of leaf lesions, and evaluating the anthracnose resistance and susceptibility characteristics of macadamia nuts based on the disease index; providing a convenient method for identifying anthracnose-resistant macadamia nut varieties.

The present invention belongs to the technical field of pesticide R & D, and specifically relates to a medicament composition for controlling the fruit borer of Macadamia integrifolia. A medicament composition for controlling the fruit borer of Macadamia integrifolia, the active ingredients of which are composed of a binary compound of flupyradifurone, clothianidin or metaflumizone and cyflumetofen. The present invention combines cyflumetofen with existing chemical medicaments, and screens out a compound combination of a binary compound of flupyradifurone, clothianidin or metaflumizone and cyflumetofen. The co-toxicity coefficient of this compound combination against the fruit borer of Macadamia integrifolia is greater than 120, showing a synergistic effect, which can improve the control effect and is of great significance for the development of highly effective medicaments for controlling the fruit borer of Macadamia integrifolia.

The present invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition for preventing and controlling red-brown spot disease of macadamia nuts. A bactericidal composition for preventing and controlling red-brown spot disease of macadamia nuts, the active ingredients of which are compounded by berberine and zhongshengmycin, fenoxanil or isopyrazam. The bactericidal composition of the present invention has a synergistic effect on inhibiting the growth of the pathogen of red-brown spot disease of macadamia nuts. Compared with a single active ingredient, it can improve the control effect on red-brown spot disease of macadamia nuts and reduce the control cost.

The present invention provides a method for detecting the self-fertility of macadamia nuts, which belongs to the field of biological detection technology. The invention provides a method for detecting the self-fertility of macadamia nuts. First, the inflorescence of the self-pollinating pistil is arranged, and then the inflorescence of the macadamia nut variety to be tested is bagged. After 7 to 9 days, the inflorescence is taken out from the bag, and all the pistils attached to the inflorescence axis together with the inflorescence axis are immersed in FAA fixative for fixation. After washing and softening, the ovary and the lower half of the style are cut horizontally and retained. The retained part is then cut longitudinally and stained. The growth of the pollen tubes in the ovary and the lower half of the style after staining is observed under a fluorescence microscope, and the PLS is counted. Using the detection method of the present invention, the self-incompatibility intensity of different varieties of macadamia nuts can be intuitively and quickly identified. The identification workload is small, accurate and reliable, saving a lot of time and economic costs, and has broad application prospects.