48 results about "Fatty acid synthesis" patented technology

The invention belongs to the technical field of biology, and discloses a yarrowia lipolytica engineering strain for efficiently producing eicosapentaenoic acid based on citric acid metabolism regulation, a method and application of the yarrowia lipolytica engineering strain. Delta9 elongase, Delta8 desaturase, Delta5 desaturase and Delta17 desaturase genes in a fatty acid synthesis route, heterologous ATP-citrate lyase and a mitochondrial citric acid vector are overexpressed. According to the invention, ATP-citric acid lyase and a mitochondrial citric acid carrier gene which are respectively derived from Schizochylene sp. And Saccharomyces cerevisiae are jointly expressed in the yarrowia lipolytica engineering strain, so that the yields of EPA and grease in the yarrowia lipolytica engineering strain are remarkably improved, and compared with a control strain, the yields of the grease and the EPA are respectively improved by 243.35% and 161.85%.

The invention belongs to the technical field of biology, and discloses an exogenous cofactor enhanced yarrowia lipolytica strain, a method and application in efficient synthesis of EPA (eicosapentaenoic acid) and lipids.The chassis strain of the yarrowia lipolytica strain is yarrowia lipolytica Po1f, desaturase is partially prolonged in an overexpression fatty acid synthesis route in the strain, and the EPA and the lipids.The yield of the EPA and the lipids.The yield of the EPA and the lipids.The yield of the EPA and the lipids. The desaturase gene mainly comprises a delta 9 elongase gene, a delta 8 desaturase gene, a delta 5 desaturase gene and a delta 17 desaturase gene, and G6PDH and malic enzyme are overexpressed at the same time on the basis. The strain provided by the invention improves the EPA and lipid yield of yarrowia lipolytica; the method is suitable for constructing a genetic engineering strain with high yield of EPA and lipid, provides a basis for producing grease rich in EPA from yarrowia lipolytica, and solves the problems of non-sustainability, high cost and the like caused by obtaining EPA from fish.

The application provides a method for continuous synthesis of medium-chain fatty acids, and belongs to the technical field of biochemistry and resource recycling. The application provides a method for continuous synthesis of medium-chain fatty acids, which comprises the following steps: mixing pretreated vinasse and a polyvinyl alcohol-sodium alginate composite matrix to obtain a fermentation matrix; adding the fermentation matrix to a fermentation system to perform pre-acidification; after the pre-acidification is completed, adjusting the feeding and discharging of the fermentation system every day, adjusting the material residence time to 9-15 days through the discharging amount, controlling the pH of the top region of the fermentation system to be 4.0-5.5 and / or the ORP to be-350--150 mV, and constructing an axial coupling system of the lactic acid production area of the top hydrolysis acidification area and the medium-chain fatty acid synthesis area of the bottom region of the fermentation system. The method does not need to add exogenous inoculum, and can realize the continuous synthesis of medium-chain fatty acids through partition regulation.

The invention relates to the technical field of gene engineering, and discloses a recombinant strain, a construction method and application thereof, and a method for producing total fatty acid. The recombinant strain is obtained by genetic modification of an original strain, and compared with the original strain, the recombinant strain overexpresses citric acid transporter Yhm2p, cis-aconitase E.HA and isocitrate dehydrogenase IDP located in cytoplasm at the same time, and does not express isocitrate dehydrogenase IDH1 and isocitrate dehydrogenase IDH2 located in mitochondria. The recombinant strain provided by the invention forms a high-efficiency pathway of citric acid retention-cytoplasm transport-directional production of NADPH, so that the core bottleneck of insufficient supply of cytoplasm NADPH in fatty acid synthesis is solved, the total fatty acid yield is remarkably increased, the transformation logic is accurate, and a stable and reliable source is provided for industrial high-efficiency production of fatty acid.

The invention belongs to the technical field of biochemical engineering, and discloses a method for promoting schizochytrium limacinum to quickly accumulate Omega-3 fatty acid by using a natural antioxidant and application of the schizochytrium limacinum, the method comprises the following steps: performing fermentation culture on the schizochytrium limacinum, adding a mixed exogenous antioxidant melatonin and a rosemary extract in the fermentation culture process, and performing fermentation culture on the mixed exogenous antioxidant melatonin and the rosemary extract to obtain the Omega-3 fatty acid-containing schizochytrium limacinum. The melatonin and rosemary extract mixed antioxidant can interact to regulate fermentation in schizochytrium limacinum to increase fatty acid synthesis and oxidation stability. According to the present invention, in the experiment of schizochytrium limacinum fermentation oil production, the addition of the specific amount of the mixed antioxidant composition during the fermentation results show that the oil yield can be increased, the PUFA ratio in the specific concentration range can be significantly increased by 32%, the PUFA use amount can be reduced by 40% through the compounding use, and the method can save manpower, material resources and cost, and can provide the good application prospect. The grease production efficiency is greatly improved, and the method is suitable for industrial production.

The application belongs to the technical field of biology, and discloses a Yarrowia lipolytica engineering strain for efficiently producing eicosapentaenoic acid based on citric acid metabolism regulation, a method and application, wherein a chassis strain of the engineering strain is Yarrowia lipolytica Po1f, and genes of Δ9 elongase, Δ8 desaturase, Δ5 desaturase and Δ17 desaturase in a fatty acid synthesis pathway, a heterologous ATP-citrate lyase and a mitochondrial citrate carrier are overexpressed. ATP-citrate lyases and mitochondrial citrate carrier genes respectively derived from Schizochytrium sp. and Saccharomyces cerevisiae are combined to be expressed in the Yarrowia lipolytica engineering strain, so that the production of EPA and oil in the Yarrowia lipolytica engineering strain is significantly improved, and compared with a control strain, the production of oil and EPA is respectively increased by 243.35% and 161.85%.

Disclosed in the invention are a type of compounds having activity of activating 5′-adenosine monophosphate-activated protein kinase (AMPK), a method for the preparation thereof, a pharmaceutical composition comprising the same, and use of these compounds in the manufacture of a medicament for reducing fatty acid synthesis, for inhibiting triglyceride and cholesterol synthesis, for preventing and / or treating obesity and type II diabetes, for preventing and / or treating tumor, for preventing and / or treating Parkinson's disease, for preventing and / or treating Alzheimer's disease or for prolonging the lifespan of mammals:

Cancers develop specific metabolic adaptations such as the opportunistic utilization of acetate as an alternative carbon source and the generation of acetyl-CoA mediated by ACSS-2, which is upregulated in response to nutrient and hypoxic stress. This pathway can fuel various processes involving acetyl-CoA, including de novo fatty acid synthesis and protein acetylation. Provided herein are methods of treating a cancerous disease in a subject using compounds that inhibit ACSS-2.

The invention discloses a pharmaceutical composition for improving glucose and lipid metabolism disorder by combining N-p-coumaroyl octoamine and rotundine and application of the pharmaceutical composition, and belongs to the field of biological medicine and metabolic disease treatment. In a db / db diabetic mouse model, it is found that NTPC (10 mg / kg) or ROT (10 mg / kg) single drugs can improve hyperglycemia and insulin resistance and reduce blood fat, and low-dosage combination (5 mg / kg of NTPC and 5 mg / kg of ROT) shows the most significant metabolism improvement effect which is obviously better than that of a single drug group. In an in-vitro fatty acid stimulation model, lipid accumulation in cells can be more remarkably reduced through combination of the two drugs, and fatty acid synthesis genes are inhibited.

The present invention provides the use of fatty acid synthase inhibitors for the prevention and treatment of drug-induced liver injury (DILI), relating to the field of biotechnology. This invention demonstrates that increased fatty acid synthesis mediated by FASN regulates macrophage inflammatory responses and migratory chemotactic behavior. Targeting macrophage fatty acid synthesis can alleviate acute liver injury and inflammatory responses. This discovery represents the first discovery of new targets and new efficacy for fatty acid synthase inhibitors, potentially resolving the current lack of specific treatments for acute liver injury.

The present invention relates to the field of sludge treatment technology, and in particular to a process for promoting sludge hydrolysis and medium-chain fatty acid synthesis using ferrocene, comprising: S10, adding ferrocene to the sludge and adjusting the pH to 3.0-5.0; S20, adding hydrogen peroxide to carry out a Fenton-like reaction, wherein the ferrocene undergoes a solid-liquid phase transition during the reaction; S30, adding ethanol, anaerobic seed sludge, and culture medium to carry out anaerobic fermentation and carbon chain extension; ferrocene generates hydroxyl radicals in the Fenton-like reaction to destroy sludge flocs and cell wall membranes; the converted liquid ferrocene cation acts as an electron mediator in step S30 to improve electron transfer efficiency; the liquid ferrocene cation simultaneously inhibits ethanol oxidation and methanogenesis. The present invention combines the Fenton-like reaction with a biological anaerobic process to construct a sludge hydrolysis, fermentation, and carbon chain extension process based on ferrocene regulation, thereby simultaneously promoting sludge hydrolysis, enhancing medium-chain fatty acid synthesis, and inhibiting substrate competition reactions, thereby expanding the high-value recovery rate of sludge resources.

The present invention provides a spirotetramat / SPc / ds Dpp Multi-component compound insecticide and preparation method thereof, the compound insecticide comprising spirotetramat / SPc complex and ds Dpp The SPc and ds in the spirotetramat / SPc complex Dpp The mass ratio of ds is 0.1-10; Dpp The nucleotide sequence of the nucleotide sequence is shown in SEQ ID NO. 1. The spirotetramat / SPc / ds Dpp ds in multi-component compound insecticides Dpp Silencing of fatty acid synthesis and epidermal formation by RNAi Dpp Gene, leading to aphid molting failure and epidermal defects; SPI further destroys the epidermal barrier by inhibiting lipid metabolism. The combination of the two significantly increases the mortality rate to 94.5-96.6% and shortens the initial onset time.

The invention relates to the technical field of sludge treatment, in particular to a process for promoting sludge hydrolysis and medium-chain fatty acid synthesis by using ferrocene, which comprises the following steps: S10, adding ferrocene into sludge and adjusting the pH value to 3.0-5.0; s20, hydrogen peroxide is added for a Fenton-like reaction, and ferrocene is subjected to solid-liquid form transformation in the reaction process; s30, adding ethanol, anaerobic seed sludge and a culture medium for anaerobic fermentation and carbon chain extension; the ferrocene generates hydroxyl radicals in the Fenton-like reaction to destroy sludge flocs and cell wall membranes; the converted liquid ferrocene cation is used as an electron mediator in the step S30 to improve the electron transfer efficiency; the liquid ferrocene cations simultaneously inhibit ethanol oxidation and methanogenesis processes. A Fenton-like reaction and a biological anaerobic process are combined, a sludge hydrolysis fermentation-carbon chain extension process based on ferrocene regulation and control is constructed, sludge hydrolysis promotion, medium-chain fatty acid synthesis enhancement and substrate competition reaction inhibition are synchronously realized, and the high-value recovery rate of sludge resources is increased.

The invention relates to the technical field of molecular biology, in particular to an MUC15 gene segment, expression vector construction of the MUC15 gene segment and an expression vector transfection method of the MUC15 gene segment, and the nucleotide sequence of the MUC15 gene segment influencing buffalo mammary epithelial cell triglyceride and fatty acid synthesis is shown as SEQ No.1. The MUC15 gene segment is obtained by amplifying a primer pair, and the primer pair is used for amplifying the buffalo mammary epithelial cell triglyceride and fatty acid synthesis. The primer pair is specifically as follows: F: 5 '-AGTCCGGACTCAGATCTCGAGATGTTGACTCAGCCAAAATTCTG-3', F: 5 '- And R: 5 '-AATTCGAAGCTTGAGCTCGAGCTTACACGAAGTGGAGG-3'. Compared with the prior art, the buffalo mammary epithelial cell MUC15 gene fragment influencing the synthesis of triglyceride and fatty acid in the buffalo mammary epithelial cell is obtained, and a pEGFP-C1-MUC15 eukaryotic expression vector is constructed and transfected to the buffalo mammary epithelial cell, so that the synthesis of triglyceride by the buffalo mammary epithelial cell is promoted, and the milk fat content in buffalo milk is increased.

Furan fatty acid synthesis compounds and furan fatty acid - amino acid conjugates are disclosed herein. Also disclosed herein are methods for treating non - alcoholic steatohepatitis (NASH) in a subject, comprising administering to the subject a therapeutically effective amount of any of the compounds disclosed herein. Also disclosed herein are methods for treating TG - induced pancreatitis and monogenic lipid disorders in a subject, comprising administering to the subject a therapeutically effective amount of any of the compounds disclosed herein.

The application discloses a tomato SlAP2 gene for increasing the content of five long-chain fatty acids in plants and application thereof, and belongs to the technical field of genetic engineering.The four SlAP2 transcription factors are overexpressed in Nicotiana benthamiana driven by a constitutive promoter 35S, and through comparison, it is found that overexpression of the tomato Sl03gAP2 and Sl06gAP2 genes can increase the content of five long-chain fatty acids, i.e., hexadecanoic acid (C16:0), hexadecatrienoic acid (C16:3), octadecanoic acid (C18:0), octadecatrienoic acid (C18:3) and eicosanoic acid (C20:0) in tobacco leaves.Through exploration of the action of the tomato Sl03gAP2 and homologous genes in regulating the synthesis of fatty acids, it is determined that the tomato Sl03gAP2 and Sl06gAP2 can be applied to the synthetic biology research of fatty acids.

The invention discloses a fermentation process and application of algal oil containing specific DHA distribution characteristics, and belongs to the technical field of microbial fermentation and functional algal oil preparation. According to the method, sodium citrate participating in tricarboxylic acid circulation is utilized to supplement and enhance central carbon metabolic flux, and more sufficient acetyl coenzyme A and precursor substances are provided for fatty acid synthesis, so that the fatty acid synthesis flux is up-regulated; the phytosterol is used as a cell membrane fluidity regulator, so that the activity of the Sn-2 site acyltransferase in the schizochytrium limacinum is favorably activated, and the DHA is directionally guided to be combined to the Sn-2 site of the TAG. According to the invention, the two substances are synergistically added to a schizochytrium limacinum fermentation system, so that the three targets that the total DHA content gt is 40%, the ratio of Sn-2DHA to the total DHA gt is 50% and the specific triglyceride molecule content reaches the standard can be simultaneously realized accidentally, and the problem that the DHA content and distribution are difficult to regulate and control synchronously in the prior art is effectively solved.

The present invention relates to proteins or polypeptides involved in fatty acid synthesis, such as fatty acid synthase (FAS). Said protein comprises one or more polypeptide chains wherein said polypeptide chains comprises one or more subunits having at least one amino acid substitution as defined resulting in altered activity of fatty acid synthesis. The present invention relates further to nucleic acid molecules encoding the proteins or the polypeptide domains as well to host cells containing said nucleic acid molecules, e.g. in form of vectors. The present invention describes further a method for production of fatty acids comprising the cultivation of the host cells according to the present invention as well as the use of the protein according to the present invention or the nucleic acid according to the present invention or the host cell according to the present invention for the production of biofuels, the production of fine chemicals, flavoring compounds, the determination of fungicides and actinobacterial biocides. Moreover, in silico methods are described allowing the identification of molecules capable of selectively altering the activity of an enzymatic domain present in the FAS or altering a step of fatty acid synthesis by FAS I comprising determining and modelling molecules interacting under physiological conditions with at least one amino acid in a protein as defined herein. Further, a method of identifying a compound capable of altering at least one of the enzymatic activities of FAS is provided including the step of bringing into contact a candidate compound with the FAS as defined herein and determining whether said candidate demonstrates any interaction with the FAS. Finally, a protein is provided, namely, a mutated ACP which results in alteration of FAS activity.

The present invention relates to methods and compositions for treatment and alleviation of diseases. Particularly, the invention provides compositions comprising very long chain fatty acids for use in treatment, such as of subjects having a deficient or abnormal level of VLCFAs present in specific tissue which play a role in the disease. Particularly, the invention provides methods and compositions for treatment of subjects having a reduced ability for endogenic synthesis of fatty acids.

The present application relates to a kind of synthetic polyunsaturated fatty acid binding phospholipid yeast engineering bacteria and construction method and application, belong to microbial engineering technical field, the engineering bacteria is with Yarrowia lipolytica as bottom cell, knock out the Mhy1, Iro1, IDH1, CEX1 gene in its genome for generating mycelium, synthesis of triglyceride, citric acid metabolism, citric acid efflux;Phospholipid synthesis, pyruvate synthesis, polyunsaturated fatty acid synthesis and acyltransferase CDS1, OPI3, Mce2, LplA, △9Elo, △8Des, △5Des, △17Des, △5Elo, △15Des, LPCAT3 gene are sequentially connected together, as an expression module is integrated in the URA site of genome.The yeast engineering bacteria of the present application obtains polyunsaturated fatty acid binding phospholipid in one step efficiently, and phospholipid yield is 151.19 mg / g, and in binding fatty acid, EPA content is 13.2%.

The invention provides a method for continuously synthesizing medium-chain fatty acid, and belongs to the technical field of biochemistry and resource recycling. The invention provides a method for continuously synthesizing medium-chain fatty acid, which comprises the following steps: mixing pretreated distillers' grains with a polyvinyl alcohol-sodium alginate composite matrix to obtain a fermentation matrix; adding the fermentation substrate into a fermentation system for pre-acidification; after pre-acidification is completed, adjusting feeding and discharging of the fermentation system every day, adjusting the retention time of the materials to be 9-15 days through the discharging amount, controlling the pH value of the top area of the fermentation system to be 4.0-5.5 and / or ORP to be-350-150 mV, and constructing an axial coupling system of a top hydrolytic acidification lactic acid production area and a bottom partition medium-chain fatty acid synthesis area of the fermentation system. According to the method, exogenous inoculums do not need to be added, and continuous synthesis of the medium-chain fatty acid can be achieved through partition regulation and control.

The invention discloses Yarrowia guilliermondii, a preparation method of an inactivated vaccine of the Yarrowia guilliermondii and application of the Yarrowia guilliermondii in prevention and treatment of alcoholic liver diseases, and belongs to the field of microorganism eukaryotes, the preservation date of the Yarrowia guilliermondii is August 18, 2025, and the preservation number is CGMCC NO. According to the present invention, the vaccine is prepared by separating and inactivating the Yarrowia guilliermondii ML001, and the vaccine can significantly reduce the alcohol-induced ALT and AST levels in mice, reduce the liver lipid accumulation, reduce the fatty acid synthesis genes Fasn and Acly, and increase the fatty acid oxidation genes Ppara and Cpt1a so as to effectively inhibit the lipid synthesis and activate the fatty acid oxidation, moreover, the traditional Chinese medicine composition has no obvious toxicity to main organs such as heart, spleen, lung, kidney and the like, and shows good safety and clinical transformation potential.

The invention relates to a method for enhancing the fatty acid synthesis capability of escherichia coli through overexpression rfaY. According to the invention, a recombinant plasmid pRF for co-overexpressing an rfaY gene and a teA'gene is constructed, and the recombinant plasmid pRF is transformed into an Escherichia coli MG1655 (DE3) [delta] fadE chassis strain for knocking out a fatty acid degradation pathway key gene fadE, so that an engineering strain RF capable of remarkably increasing the yield of fatty acid is obtained. The fatty acid yield of the engineering strain RF reaches 2461.29 mg / L and is improved by 208% compared with that of a control strain F. RfaY (coding lipopolysaccharide core heptase II phosphokinase) participates in synthesis of lipopolysaccharide which is a main component of an extracellular membrane, and further cell membrane characterization shows that the cell membrane integrity of the engineering strain RF is improved by 81.0% compared with that of a control strain F. The invention provides a simple and convenient construction method of a high-yield fatty acid strain, and the fatty acid synthesis capability of escherichia coli is remarkably improved.

The present invention provides a protein for promoting early flowering and altering fatty acid composition, its coding gene and applications, belonging to the field of biotechnology, and specifically discloses the application of the gene of Macadamia integrifolia in promoting early flowering and altering fatty acid composition. By studying the gene of the NF-YB family in Macadamia integrifolia, the present invention helps to understand the flowering and fatty acid synthesis mechanisms of Macadamia integrifolia, and uses genetic engineering techniques and the like to induce plants to flower early and alter fatty acid composition, thereby providing a theoretical basis and reference for the research on the mechanisms of regulating flowering and fatty acid synthesis accumulation. DRAP1 and its coding gene and applications, belonging to the field of biotechnology, and specifically discloses the application of the gene of Macadamia integrifolia in promoting early flowering and altering fatty acid composition. DRAP1 By studying the gene of the NF-YB family in Macadamia integrifolia, the present invention helps to understand the flowering and fatty acid synthesis mechanisms of Macadamia integrifolia, DRAP1 and uses genetic engineering techniques and the like to induce plants to flower early and alter fatty acid composition, thereby providing a theoretical basis and reference for the research on the mechanisms of regulating flowering and fatty acid synthesis accumulation.

A Grifola frondosa polysaccharide GFP22, a preparation method therefor, and the use thereof in the preparation of a prebiotic product and an auxiliary antibacterial drug. The Grifola frondosa polysaccharide GFP22 is mannoglucan polymerized from mannose and glucose in the ratio of mannose to glucose of 2.88:5.24, has the following structure, and has a weight-average molecular weight range of 4.9 kDa. The Grifola frondosa polysaccharide GFP22 can promote the synthesis of intestinal short-chain fatty acids and regulate intestinal flora homeostasis, promote the proliferation of Bifidobacteria, inhibit the proliferation of Klebsiella, and is expected to be developed into a prebiotic product and a carbohydrate drug for assisting with an anti-Klebsiella drug.

The present invention provides Lactobacillus plantarum ZJUIDS14 and its application for improving non-alcoholic liver disease, which is classified and named as: Lactiplantibacillus plantarum, and the preservation number is: CGMCC NO.28091. The strain of the present invention regulates the abundance of various intestinal probiotics in the intestine and promotes the synthesis of intestinal short-chain fatty acids to restore the intestinal barrier function damaged by a high-fat diet. In addition, ZJUIDS14 and the extracellular polysaccharides produced by it can inhibit the liver fatty acid synthesis pathway to reduce liver triglyceride deposition, and improve liver damage through antioxidant pathways. The strain has the functions of protecting liver damage, improving antioxidant effects in the body, reducing liver triglycerides, reducing liver fatty acid synthesis, restoring intestinal barrier function, promoting intestinal short-chain fatty acid synthesis, and improving intestinal flora, and can be used in the preparation of functional foods such as bacterial powder, yogurt or milk powder.

The invention provides a grass carp n-3 polyunsaturated fatty acid synthesis surplus model and a construction method thereof, and relates to the technical field of gene engineering. The nucleotide sequence of the sgRNA is as shown in SEQ ID NO. 1. The sgRNA has a sequence shown in SEQ ID NO.1, and can specifically target and effectively destroy protein coding of a second exon of a grass carp retsat. 2 gene. The accurate gene modification can significantly increase the content of n-3 polyunsaturated fatty acids in the liver, brain and muscle of the grass carp, and is a key molecular tool for obtaining the excellent nutritional character.

The invention discloses a tomato SlAP2 gene for improving the content of five long-chain fatty acids in a plant and application thereof, and belongs to the technical field of gene engineering. According to the tomato S103gAP2 and S106gAP2 gene, a constitutive promoter 35S is used for driving overexpression of four S1AP2 transcription factors in nicotiana benthamiana, and comparison shows that overexpression of tomato S103gAP2 and S106gAP2 genes can increase the content of five long-chain fatty acids in tobacco leaves, namely hexadecanoic acid (C16: 0), hexadecanoic acid (C16: 3), octadecanoic acid (C18: 0), octadecanoic acid (C18: 3) and eicosanoic acid (C20: 0). The tomato S103gAP2 and the tomato S106gAP2 are determined to be capable of being applied to the biological research of synthesis of fatty acid by exploring the effect of the tomato S103gAP2 and the homologous gene in regulating and controlling the synthesis of the fatty acid.

The application belongs to the field of microorganism eukaryotes, and the strain preservation date is August 18, 2025, and the preservation number is CGMCC NO.35650. The vaccine is prepared by isolating and inactivating the Meyerozyma guiliardmi yeast ML001, the vaccine can significantly reduce the alcohol-induced ALT and AST levels in mice, reduce liver lipid accumulation, down-regulate fatty acid synthesis genes Fasn and Acly and up-regulate fatty acid oxidation genes Ppara and Cpt1a , thereby effectively inhibiting lipid synthesis and activating fatty acid oxidation, reducing alcoholic liver injury, and having no obvious toxicity to main organs such as heart, spleen, lung and kidney, and showing good safety and clinical transformation potential.

The invention relates to the technical field of molecular biology, in particular to an ACSL3 gene segment, an expression vector construction method thereof and an expression vector transfection method thereof, the nucleotide sequence of the ACSL3 gene segment influencing buffalo mammary epithelial cell triglyceride and fatty acid synthesis is shown as SEQ No.1, the ACSL3 gene segment is obtained by amplifying a primer pair, and the primer pair has a nucleotide sequence shown as SEQ No.2. The primer pair is specifically as follows: F: 5 '-AGTCCGGACTCAGATCTCGAGATGAATAACCAGGGTCTACAA-3', F: 5 '-AG: 5'-AG: 5 '-AG: 5'-AG: 5 '-AG: 5'-AG: 5 ' And R: 5 '-AATTCGAAGCTTGAGCTATGAGCTATTTTCTTCCATATTCGCTCA-3'. Compared with the prior art, the ACSL3 gene fragment influencing the synthesis of the buffalo mammary epithelial cell triglyceride and fatty acid is obtained, and a pEGFP-C1-ACSL3 eukaryotic expression vector is constructed and transfected to the buffalo mammary epithelial cell, so that the synthesis of the triglyceride by the buffalo mammary epithelial cell is promoted, and the milk fat content in buffalo milk is increased.