Nucleic acid constructs and methods for producing altered seed oil compositions

a technology of nuclear constructs and modified seed oil, which is applied in the direction of enzymology, enzymology, transferases, etc., can solve the problems of insufficient availability lack of important food quality properties, and high cost of prior soybean oil, so as to increase the oleic acid content, suppress the endogenous expression, and increase the endogenous expression

Inactive Publication Date: 2006-04-13
MONSANTO TECH LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a soybean oil that is broadly beneficial to major soybean oil users such as consumers of salad oil, cooking oil and frying oil, and industrial markets such as biodiesel and biolube markets, is not available.
Prior soybean oils were either too expensive or lacked an important food quality property such as oxidative stability, good fried food flavor or saturated fat content, or an important biodiesel property such as appropriate nitric oxide emissions or cold tolerance or cold flow.

Method used

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  • Nucleic acid constructs and methods for producing altered seed oil compositions
  • Nucleic acid constructs and methods for producing altered seed oil compositions
  • Nucleic acid constructs and methods for producing altered seed oil compositions

Examples

Experimental program
Comparison scheme
Effect test

example 1

Suppression Constructs

1A. FAD2-1 Constructs

[0151] The FAD2-1A intron (SEQ ID NO: 1) is cloned into the expression cassette, pCGN3892, in sense and antisense orientations. The vector pCGN3892 contains the soybean 7S promoter and a pea rbcS 3′. Both gene fusions are then separately ligated into pCGN9372, a vector that contains the CP4 EPSPS gene regulated by the FMV promoter. The resulting expression constructs (pCGN5469 sense and pCGN5471 antisense) are used for transformation of soybean.

[0152] The FAD2-1B intron (SEQ ID NO: 2) is fused to the 3′ end of the FAD2-1A intron in plasmid pCGN5468 (contains the soybean 7S promoter fused to the FAD2-1A intron (sense) and a pea rbcS 3′) or pCGN5470 (contains the soybean 7S promoter fused to the FAD2-1A intron (antisense) and a pea rbcS 3′) in sense or antisense orientation respectively. The resulting intron combination fusions are then ligated separately into pCGN9372, a vector that contains the CP4 EPSPS gene regulated by the FMV promot...

example 2

Combination Constructs

[0161] In FIGS. 7-15, promoters are indicated by arrows, encoding sequences (both coding and non-coding) are indicated by pentagons which point in the direction of transcription, sense sequences are labeled in normal text, and antisense sequences are labeled in upside-down text. The abbreviations used in these Figures include: 7Sa=7Sα promoter, 7Sa′=7Sα′ promoter, Br napin=Brassica napin promoter; FMV=an FMV promoter; ARC=arcelin promoter; RBC E9 3′=Rubisco E9 termination signal; Nos 3′=nos termination signal; TML 3′=tml termination signal; napin 3′=napin termination signal; ‘3 (in the same box as FAD or FAT)=3′ UTR, 5′ (in the same box as FAD or FAT)=5′UTR; Cr=Cuphea pulcherrima; Gm=Glycine max; Rc=Ricinus communis; FAB2=a FAB2 allele of a stearoyl-desaturase gene; and Intr or Int=intron.

2A. dsRNA Constructs

[0162]FIGS. 7-9 depict nucleic acid molecules of the present invention in which the first sets of DNA sequences are capable of expressing dsRNA constru...

example 3

Plant Transformation and Analysis

[0200] The constructs of Examples 1 and 2 are stably introduced into soybean (for example, Asgrow variety A4922 or Asgrow variety A3244 or Asgrow variety A3525) by the methods described earlier, including the methods of McCabe et al., Bio / Technology, 6:923-926 (1988), or Agrobacterium-mediated transformation. Transformed soybean plants are identified by selection on media containing glyphosate. Fatty acid compositions are analyzed from seed of soybean lines transformed with the constructs using gas chromatography. In addition, any of the constructs may contain other sequences of interest, as well as different combinations of promoters.

[0201] For some applications, modified fatty acid compositions are detected in developing seeds, whereas in other instances, such as for analysis of oil profile, detection of fatty acid modifications occurring later in the FAS pathway, or for detection of minor modifications to the fatty acid composition, analysis of ...

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Abstract

The present invention is in the field of plant genetics and provides recombinant nucleic acid molecules, constructs, and other agents associated with the coordinate manipulation of multiple genes in the fatty acid synthesis pathway. In particular, the agents of the present invention are associated with the simultaneous enhanced expression of certain genes in the fatty acid synthesis pathway and suppressed expression of certain other genes in the same pathway. Also provided are plants incorporating such agents, and in particular plants incorporating such constructs where the plants exhibit altered seed oil compositions.

Description

FIELD OF THE INVENTION [0001] The present invention is directed to recombinant nucleic acid molecules, constructs, and other agents associated with the coordinate manipulation of multiple genes in the fatty acid synthesis pathway. In particular, the agents of the present invention are associated with the simultaneous enhanced expression of certain genes in the fatty acid synthesis pathway and suppressed expression of certain other genes in the same pathway. The present invention is also directed to plants incorporating such agents, and in particular to plants incorporating such constructs where the plants exhibit altered seed oil compositions. BACKGROUND [0002] Plant oils are used in a variety of applications. Novel vegetable oil compositions and improved approaches to obtain oil compositions, from biosynthetic or natural plant sources, are needed. Depending upon the intended oil use, various different fatty acid compositions are desired. Plants, especially species which synthesize ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A01H5/00A01H1/00C12N15/82C12N5/04C12N9/10C12P21/02
CPCA23D9/00A23K1/14A23K1/1631A23K1/164C10L1/026C11B1/00C12N9/0083C12N15/8237C12N15/8247Y02E50/13A23K10/30A23K20/147A23K20/158Y02E50/10
Inventor FILLATTI, JOANNEBRINGE, NEALDEHESH, KATAYOON
Owner MONSANTO TECH LLC
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