779 results about "Plant genes" patented technology

The present invention relates to the fields of genetic engineering and plant genetic breeding, and specifically to the application of wheat TaPND gene. In the present invention, by taking gene TaPND as a target, designing a sgRNA sequence based on CRISPR / Cas9, connecting a DNA fragment containing the encoding sgRNA sequence to a vector carrying CRISPR / Cas, a plant binary expression vector capable of knocking out the TaPND gene in wheat is constructed; by genetically transforming wheat, offspring with TaPND gene knockout are obtained, and wheat mutants with early flowering time are obtained. Experiments have confirmed that transgenic wheat with TaPND gene knockout achieves the advancement of wheat flowering time. The technical scheme of the present invention provides a practical method for realizing rapid wheat breeding using genetic engineering technology, which has important breeding application value and broad market application prospects.

Provided are compositions for genome editing and site-directed integration in plants comprising microprojectile particles coated, treated of applied with a recombinant DNA construct comprising a sequence encoding one or more genome editing reagents for delivery to a mature embryo explant from dry seeds. Further provided are methods for genome editing and site-directed integration in at least one cell of a plant using the disclosed compositions, and plants, plant parts and seeds comprising an edited genome or site-directed integration, which are produced by the disclosed methods.

The invention discloses a plant-derived Argonaute protein and application thereof, and belongs to the technical field of gene editing and detection. The plant-derived Argonaute protein is specifically a protein with an amino acid sequence as shown in SEQ ID NO.1, or a protein which has at least 80% of homology with the amino acid sequence as shown in SEQ ID NO.1 and has corresponding functions; the Argonaute protein specifically cleaves target nucleic acid under mediation of guide nucleic acid, the guide nucleic acid is guide DNA or guide RNA, and the target nucleic acid is a DNA target or an RNA target. Compared with the reported eukaryotic Argonaute protein derived from unicellular organisms, the Argonaute protein used in the invention is derived from rice, has greater application potential in the field of animal and plant gene editing, not only has RNase activity and activity of cutting a DNA target by utilizing guide RNA, but also can cut the DNA target by utilizing guide DNA, so that the scheme of the invention has good application prospect.

This document relates to methods and materials for genome engineering in eukaryotic cells, and particularly to methods for increasing genome engineering (i.e. transformation or genome editing) efficiency via delivery of one or more booster polypeptides, and boost genes, with genome engineering components.

The invention relates to the technical field of collaborative prediction, in particular to a plant polygene stress resistance collaborative prediction method and system based on a federal map neural network. The method comprises the following steps: modeling a multilayer heterogeneous graph according to acquired plant genome data; constructing a dynamic graph neural network model based on time sequence perception; training a dynamic graph neural network model by using the multi-layer heterogeneous graph, and performing distributed privacy calculation on the multi-layer heterogeneous graph by using a federated graph neural network; generalization training is carried out on the small sample scene data based on multi-task learning and a cross-species migration mechanism; and obtaining a gene function prediction result. Through a multi-layer heterogeneous graph fusion technology and a dynamic graph neural network design of time sequence perception, multi-dimensional biological information such as gene regulation, protein interaction, metabolic pathways and stress response can be captured at the same time, and time sequence change characteristics of gene expression in the plant stress response process can be captured at the same time.

The invention relates to the field of plant gene map-based cloning and molecular breeding. Specifically, the invention provides a nucleic acid molecule and a polypeptide, and also provides a recombinant vector, a host cell and a plant or a part, a seed, a cell or a progeny thereof containing the nucleic acid molecule. Furthermore, the invention also provides a method for preparing the transgenic plant, and a method for regulating and controlling the nitrogen utilization efficiency, nitrate absorption or transport, biomass and / or yield of the plant.

The invention belongs to the technical field of plant gene engineering, and particularly relates to an OsNUC1 gene and application of a gene mutant constructed by the OsNUC1 gene to regulation and control of rice plant height, leaf length and panicle type. The regulation is to regulate the plant height and leaf growth of the rice and influence the shape of the ear part. Compared with the prior art, the OsNUC1 gene mutant has the following advantages: (1) the OsNUC1 is directionally edited by utilizing a CRISPR / Cas9 gene editing technology, an OsNUC1 gene mutant rice material is obtained, the biological phenotype of the OsNUC1 gene mutant rice material is investigated, the phenotype investigation on T1-generation seedlings of the OsNUC1 shows that the plant heights and the leaf lengths of one-week-old and two-week-old seedlings of the mutant are obviously higher than those of wild seedlings, the leaf widths have no obvious difference, and the OsNUC1 gene mutant rice material has a good application prospect; the result shows that the OsNUC1 can regulate the plant height and the leaf growth of the rice and influence the shape of the ear part. (2) it is proved that the nucleolin gene OsNUC1 is negatively regulated by a transcription factor OsARF7, and the phenotypes such as the plant height, the leaf length, the grain length and the grain width of the OsNUC1 / OsARF7 mutant are more obvious than those of the OsNUC1 mutant. The invention further proves the application of the OsNUC1 gene in regulation and control of rice plant height and leaf growth.

The present invention relates to the field of genetic engineering. Specifically, the present invention relates to cytosine deaminase and use thereof in base editing. More specifically, the present invention relates to a base editing system based on a newly identified cytosine deaminase, a method for base editing a target sequence in the genome of an organism (e.g., a plant) using the base editing system, and a genetically modified organism (e.g., a plant) produced by the method and progenies thereof.

The invention discloses application of arabinogalactan protein family genes PbrAGP16 in promoting growth of pear pollen tubes, and belongs to the technical field of plant genetic engineering. According to the invention, the gene PbrAGP16 is cloned from pollen of Dangshan pears by using a plant gene cloning technology, and the nucleotide sequence of the gene PbrAGP16 is shown as SEQ ID No.1; the amino acid sequence coded by the gene is SEQ ID No.2 in a sequence table. Researches show that PbrAGP16 can promote growth of pear pollen tubes, the change of PbrAGP16 expression is related to the change of the content of cellulose at the top ends of the pollen tubes, and PbrAGP16 also participates in a regulation mechanism of a self-incompatible reaction. The pollen magnetic transfection technology is adopted to research the function of the gene in the pollen tube, and a wide application prospect is provided for improving the pollination efficiency.

The invention discloses a gene editing system and application thereof in efficient editing of plant genes, gRNA delivered by TRV (transient receptor virus) enters most of tissues or cells of explants, a large number of copies can be generated by self-replication of the virus, subgenome promoters on the copies drive expression of the gRNA, and the expression of the gRNA is driven by the subgenome promoters on the copies. Compared with a conventional III type promoter integrated into a genome, the expression quantity of gRNA is qualitatively improved, and the gene editing efficiency can be remarkably improved.

The invention provides a structure, a sequence and a construction method of a plant virus gene editing vector and application of the plant virus gene editing vector in plant gene editing. The method is characterized in that a TRV2 genome of a tobacco embrittlement virus (TRV) is used for carrying and expressing a small Cas gene AsCas12f and gRNA of the small Cas gene AsCas12f. Preferably, the AsCas12f and the gRNA of the AsCas12f are subjected to tandem expression by utilizing the TRV2 to form an expression cassette structure of the AsCas12f-tRNA-gRNA, the expression cassette substitutes a part of 2b gene sequence of the TRV2 on a TRV2 genome to form a plant gene editing vector pTRV2-Cas12f-gRNA based on the TRV virus, and long-time stable expression and gene editing can be performed after wild type nicotiana benthamiana is inoculated. The invention also provides a method for carrying out gene editing in a wild type plant by utilizing the plant virus.

The invention discloses an application of a gene GmAGO5 of a soybean ARGONAUTE family. The gene GmAGO5 of the soybean ARGONAUTE family is shown The nucleotide sequence of the soybean GmAGO5 protein coding gene GmAGO5 is as shown in SEQ ID NO. 1. The invention also discloses a preparation method of the soybean GmAGO5 protein coding gene The constructed plant gene editing knockout vector pSCM-GmAGO5 is transferred into an acceptor material Tianlong No.1 by using a cotyledonary node transformation method. The GmAGO5 gene is knocked out, so that the plant height of the gene edited soybean is reduced, the branch included angle is reduced, and the protein content of the soybean seeds is remarkably increased. Therefore, the soybean GmAGO5 protein coding gene GmAGO5 disclosed by the invention can be applied to reducing soybean plant height, reducing branch included angle and improving the protein content of soybean seeds through a genetic engineering means.

The invention belongs to the technical field of plant heredity and genetic engineering, and particularly relates to a wheat saline-alkaline tolerant protein kinase gene TaCRCK and application thereof. Specifically, the invention discloses a wheat saline-alkaline tolerant protein kinase gene TaCRCK, and the nucleotide sequence of cDNA of the gene is as shown in SEQ ID NO. 1. The invention also discloses a plant expression vector containing the gene TaCRCK and application of the gene and the plant expression vector in cultivation of saline-alkali resistant plants. Tests prove that silent expression of the gene promotes the resistance of wheat to saline-alkaline stress, and the saline-alkaline tolerance of transgenic plants is obviously improved. The gene has a negative regulation effect on the salt and alkali tolerance of plants, provides a new tool and method for improving the salt tolerance of crops by using a genetic engineering means, and has important scientific significance and application prospects.

The invention belongs to the technical field of plant heredity and genetic engineering, and particularly relates to a wheat saline-alkaline tolerant ERF transcription factor gene TaERF109 and application thereof. The invention discloses the wheat saline-alkali stress response gene TaERF109 obtained by cloning by utilizing the existing plant gene engineering technology for the first time, the gene is overexpressed in wheat through an agrobacterium tumefaciens mediated method, and comparative analysis proves that the saline-alkali tolerance of a transgenic plant is obviously improved, and the transgenic plant has a good application prospect. And interference on expression of the TaERF109 can reduce the resistance of the wheat to salt and alkali stress. Therefore, the invention fully proves that the wheat saline-alkaline tolerant ERF transcription factor gene plays an important role in plant saline-alkaline tolerant regulation and control, and shows that the gene can be widely applied to cultivation and improvement of plant saline-alkaline tolerant varieties, so that the wheat saline-alkaline tolerant ERF transcription factor gene has good practical application value.

The invention provides a method for realizing fixed-point activation of a plant genome by directionally inserting an enhancer, and particularly, by inserting a short enhancer (STE) in a specific region of a target gene promoter, a target gene can be efficiently activated, the expression level of the target gene can be enhanced by about 2000 times, and the enhancement multiple can be flexibly adjusted as required. The site-specific activation method for the plant genome has high efficiency, wide applicability and stable heredity, and has outstanding application value in plant research and breeding.

An expression increasing element comprising a 36 nucleotide DNA sequence that can be used to increase the expression of genes, and in particular to increase the expression of endogenous plant genes, in plants is disclosed. Also disclosed are plants, plant parts, and commodity plant products comprising the expression increasing element along with related methods of using the expression increasing element and plants comprising the expression increasing element.

The invention relates to the technical field of plant genetic breeding, in particular to a rice sheath blight resistance related gene and an encoding protein and application thereof. The invention provides a rice sheath blight resistance related gene as well as an encoding protein and application thereof, the gene is OsSBR4 or a related gene thereof, the resistance of rice to sheath blight can be remarkably enhanced by knocking out the gene, and the resistance of rice to sheath blight can be reduced by overexpressing the gene. After agronomic traits of a transgenic line of the gene are investigated, it is found that the plant height, the ear length, the ear number, the thousand seed weight and the like are not affected, and it is indicated that the gene has important application value in sheath blight resistance molecular breeding. According to the invention, a novel rice sheath blight resistance related gene is screened and excavated, and the identification of the sheath blight resistance gene can provide a theory and a basis for breeding a sheath blight resistance rice variety.

The invention discloses a molecular marker of a wheat stem rot disease-resistant site Qfcr.cau-1B and application of the molecular marker, and belongs to the technical field of molecular markers and plant genetic breeding. The nucleotide sequence of the molecular marker is as shown in SEQ ID NO.1, and T / A mutation exists at the 201st basic group of the sequence as shown in SEQ ID NO.1. A pair of STARP primer groups is designed for the molecular marker, and the primer groups can accurately detect the molecular marker. The molecular marker can be used for identifying the resistance of wheat to stem rot or screening varieties or strains which are resistant to and susceptible to wheat stem rot. The molecular marker provided by the invention can rapidly and accurately identify the resistance of wheat to FCR, and provides a very valuable molecular tool for screening wheat anti-FCR germplasm resources.

The invention belongs to the technical field of plant genetic transformation, and particularly relates to a callus induction culture medium for genetic transformation, a culture medium combination and a genetic transformation method. The culture medium composition comprises a callus induction culture medium, a recovery culture medium, a screening culture medium, a differential culture medium and a rooting culture medium, wherein each 1L of the callus induction culture medium is prepared from the following components: 5-8g of tryptone, 5-8g of yeast, 100-120mg of sodium chloride, 0.8-1g of glutamic acid, 250-300mg of monopotassium phosphate, 100-150mg of magnesium sulfate, 0.8-1mu g of biotin, 10-20g of mannitol and the balance of water. By optimizing the culture medium formula and the transformation condition, the induction efficiency, the regeneration efficiency, the transformation efficiency and the plant regeneration capacity of the wheat are improved, and the method can be widely applied to the technical field of agricultural biology, especially in research, development and production of transgenic crops, and is beneficial to rapidly cultivating new varieties with excellent characters.

The invention discloses application of a pear transcription factor PbrWHY2 gene in promoting growth of pear pollen tubes, and belongs to the technical field of plant genetic engineering. According to the invention, the gene PbrWHY2 is cloned from pollen of Dangshan pears by using a plant gene cloning technology, and the nucleotide sequence of the gene PbrWHY2 is shown as SEQ ID No.1; the amino acid sequence coded by the gene is SEQ ID No.2 in a sequence table. The PbrWHY2 can promote the growth of a pear pollen tube, the change of the expression of the PbrWHY2 is related to the change of ROS level and cellulose content at the top end of the pollen tube, and meanwhile, the PbrWHY2 also participates in a regulation mechanism of a self-incompatible reaction to regulate a downstream gene PbrAGP16 / PbrRALF6 / PbrGIS1 in a targeted manner. The pollen magnetic transfection technology is adopted to research the function of the gene in the pollen tube, and a wide application prospect is provided for improving the pollination efficiency.

The invention relates to the field of gene engineering. In particular, the present invention relates to cytosine deaminases and their use in base editing. More specifically, the invention relates to a method for screening and identifying a deaminase, a base editing system based on a newly identified cytosine deaminase, a method for editing a target sequence in a genome of an organism (such as a plant) by using the base editing system, and a method for screening and identifying the target sequence. As well as genetically modified organisms (e.g., plants) and progeny thereof produced by the method.

The invention discloses an efficient genetic transformation method of stem tumor sesbania, and belongs to the technical field of plant genetic transformation. According to the efficient genetic transformation method of the stem tumor sesbania, the cotyledonary node is used as the initial explant, callus induction is not needed, an optimized culture medium component is combined, a genetic transformation system of the stem tumor sesbania can be rapidly and effectively established, the genetic transformation efficiency can be improved and can reach 25% or above, the transformation time is shortened, and the cost is reduced. And a foundation is laid for domestication and accurate improvement work of the stem tumor sesbania.

The invention discloses a TaIMPA1 gene and application of the TaIMPA1 gene in improvement of plant genetic transformation efficiency. The nucleotide sequence of the gene is shown as SEQ ID No: 1 in a sequence table. According to the invention, the key gene TaIMPA1 is cloned from a plant body to delivery for the first time, and the genetic transformation performance is enhanced by remarkably improving the delivery efficiency of exogenous DNA (Deoxyribose Nucleic Acid). The discovery fills up the research blank of plant endogenous delivery regulatory factors, and an original solution is provided for overcoming the core problem of genotype dependence.

The invention discloses a 5 '-UTR sequence derivative based on SARS-CoV-2 and application of the 5'-UTR sequence derivative in enhancement of plant gene translation expression, and relates to the technical field of enhancement of plant gene translation expression. The 5 '-UTR sequence derivative provided by the invention is used for enhancing translation expression of a target gene in a plant body; the nucleotide sequence of the 5 '-UTR sequence derivative is as shown in SEQ ID NO. 1 or SEQ ID NO. 2. The SARS-CoV-2 virus 5 '-UTR is applied to a plant system for the first time, through the optimized 5'-UTR sequence derivative mediated translation regulation effect, the level of target gene expression protein is remarkably improved on the translation level, and the SARS-CoV-2 virus 5 '-UTR is suitable for various plant varieties such as gramineous plants, cruciferous plants and solanaceae plants.

The invention discloses a corn dwarfing control gene ZmEXO84C and application thereof, and relates to the technical field of functional gene and plant gene breeding. The nucleotide sequence of the ZmEXO84C gene is as shown in SEQ ID NO. 1 (sequence identifier number 1). The ZmEXO84C gene has relatively high expression in leaves, tassels and embryos of corn, and the amino acid sequence of the ZmEXO84C gene encoding protein is as shown in SEQ ID NO. 2. Phenotype identification is carried out on a corn EMS mutagenesis mutant, separation and population construction are carried out on the obtained dwarfing mutant d2201, genetic analysis and Mutmap positioning are combined, it is determined that ZmEXO84C is an effect gene causing dwarfing of the mutant d2201, the gene has nonsense mutation in the mutant d2201, the plant height can be remarkably reduced, and the yield of the plant is increased. The utilization of the functional gene is beneficial to improvement of corn plant height and density-tolerant breeding, and has important breeding application potential.

The invention discloses an RNA (Ribonucleic Acid) aptamer and application thereof in improving the salt tolerance of rice. The aptamer sequence eApt-2 with high affinity to eIF4A is obtained through enrichment by using a phylogenetic screening technology of the nucleic acid aptamer, and the aptamer sequence eApt-2 can inhibit the RNA helicase activity of eIF4A and selectively block a 5'cap-dependent translation initial process. After the eApt-2 coding sequence is introduced into a rice cell, the transgenic rice shows significantly enhanced salt tolerance under a salt stress condition: the growth condition is improved, and the biomass and the photosynthetic efficiency are both higher than those of a control plant. Compared with a traditional strategy of improving the salt tolerance through gene overexpression or knockout, the method does not need permanent modification of animal and plant genomes, accurate and reversible regulation and control of translation initiation factors are carried out through aptamers, optimization of stress response protein synthesis is achieved, and higher specificity and safety are achieved. The invention provides a novel and efficient technical approach for cultivating salt-tolerant crops, and has a wide application prospect.

Provided are compositions for genome editing and site-directed integration in plants comprising microprojectile particles coated, treated or applied with a nuclease protein, guide RNA or RNP for delivery to a mature embryo explant from dry seeds. Further provided are methods for genome editing and site-directed integration in at least one cell of a plant using the disclosed compositions, and plants, plant parts and seeds comprising an edited genome or site-directed integration, which are produced by the disclosed methods.

The invention discloses a method for site-specific replacement or insertion of DNA fragments in a plant genome, and belongs to the technical field of biology. The method disclosed by the invention comprises the following steps: editing a genome of a plant protoplast by adopting a CRISPR / Cas9 system and taking a double-stranded DNA repair template as a donor template to obtain an edited protoplast; and carrying out culture regeneration on the edited protoplast by utilizing a plant protoplast transformation method to obtain a site-directed mutagenesis plant in the genome so as to realize site-directed mutagenesis of the target plant genome. The method disclosed by the invention can be used for accurately replacing DNA in a plant genome at a fixed point. According to the invention, a recognition sequence of recombinase can be accurately inserted into a genome at a fixed point through homologous recombination, and then accurate fixed-point replacement of a gene large fragment is realized by virtue of a specific recombinase system.

The invention relates to application of a corn ZmECT3 gene in regulation and control of plant heat resistance, and belongs to the technical field of plant gene breeding, the nucleotide sequence of the ZmECT3 gene is as shown in SEQ ID NO.1, and the amino acid sequence of the gene encoding protein is as shown in SEQ ID NO.2. By analyzing a ZmECT3 gene deleted corn plant and a ZmECT3 gene overexpressed arabidopsis thaliana plant, it is found that the ZmECT3 gene can regulate and control the heat resistance of the plants, and important theoretical significance and application value are provided for creation of heat-resistant corn new germplasm and heat-resistant corn molecular breeding.