52 results about "Plant genes" patented technology

The present application relates to the technical field of plant genetic breeding, and particularly relates to a Brassica campestris fatty acyl reductase BcFAR2 gene, an expression protein thereof and application, a base sequence of the Brassica campestris fatty acyl reductase BcFAR2 gene is shown as SEQ ID NO. 1, and an amino acid sequence of the expression protein is shown as SEQ ID NO. 2, the Brassica campestris fatty acyl reductase BcFAR2 gene is cloned, and BcFAR2 gene overexpression Brassica campestris sterile plants can restore pollen activity; gene knockout Brassica campestris fertile plants can significantly reduce pollen activity; in Arabidopsis thaliana, overexpression of the gene can significantly improve salt tolerance of the transgenic plants; and in Brassica campestris, overexpression of the gene can significantly enhance salt tolerance.

This invention discloses a genetic transformation and gene editing method applicable to multiple sesquiterpene species, belonging to the field of plant genetic transformation and gene editing technology. The method uses the cotyledonary nodes of sterile seedlings of the sesquiterpene genus as explants, and introduces an optimized pScEF1α-Cas9 gene editing transformation vector using Agrobacterium infection. Regenerated plants are obtained through recovery culture, selection culture, and rooting culture. The vector contains four gene expression units: pScEF1α:Cas9, pGmU6:sgRNA, pGmUBI:DsRed2, and pCaMV35S:HygR. In vivo screening is further performed using the DsRed2 fluorescent reporter gene, and the gene-edited plants are identified by molecular detection. This method is applicable to common sesquiterpene, stem-nodled sesquiterpene, and spiny sesquiterpene, and has advantages such as strong species versatility, high transformation efficiency, short cycle, visualized screening, and high positive rate, providing efficient technical support for gene function research and genetic improvement of sesquiterpene species.

The present invention relates to a method for preparing a MAGEA8-Fc transgenic plant. Through a platform using a tobacco plant, a recombinant protein MAGEA8-Fc, in which a human IgG Fc fragment is fused to a MAGEA8 protein, can be stably expressed. The method of the present invention enables high-level expression efficiency and stable protein production by introducing a target gene into a plant genome using an Agrobacterium binary vector. By directly isolating and purifying the recombinant protein from the transgenic tobacco plant, large-scale production can be easily achieved. Compared to conventional microbial or animal cell-based expression systems, the method provides economic efficiency and scalability, and thus can be usefully applied as a platform for producing fusion proteins.

This invention relates to the field of plant gene extraction technology, specifically to a ligase-based plant gene fragment screening and filtering device. The device includes a workbench, a support frame, a mounting column, a mounting plate, a turntable, and an extraction device. The support frame is fixedly connected to the lower surface of the workbench, the mounting column is fixedly connected to the upper surface of the workbench, and the mounting plate is fixedly connected to the upper surface of the mounting column. The turntable is disposed inside the workbench and is rotatably connected to the surface of the mounting column. The extraction device is mounted on the turntable and includes a support plate and a groove. The support plate is fixedly connected to the lower surface of the workbench. This invention, by setting up the extraction device, allows operators to complete all extraction steps from a single location, reducing operator fatigue and physical exertion. Furthermore, with the addition of four sets of dissolving cups, continuous processing of plant fragments can be achieved, effectively improving the convenience of the equipment.

This invention relates to a genetic transformation method for Ningxia wolfberry using germinating seeds as explants, belonging to the field of plant genetic transformation technology. The invention mainly includes the following steps: (1) After disinfecting Ningxia wolfberry seeds, inoculate them onto a germination medium, and obtain germinating seeds through vernalization and dark culture treatment; (2) Activate and culture Agrobacterium rhizogenes and Agrobacterium tumefaciens strains carrying target gene expression frames to obtain a bacterial solution for infection; (3) Take the germinating seeds obtained in step (1) and mix them with the bacterial solution obtained in step (2) for infection, then transfer the infected germinating seeds to a co-culture medium or substrate for co-culture; (4) Cultivate the material after co-culture in step (3) to induce root growth, and detect the expression level of the target gene or reporter gene on the roots or plants to obtain transgenic positive plants. This invention has the advantages of simple operation, significantly shortened transformation cycle, and high transformation efficiency.

The present application relates to plant RL1 gene regulation broad spectrum disease resistance and application. The present application provides a RL1 down-regulation agent for use in improving plant disease resistance, or for preparing a preparation for enhancing plant disease resistance. The present application also provides a method for improving traits of a plant in the family Poaceae or preparing a plant in the family Poaceae with improved traits, comprising: down-regulating the expression or activity of RL1 in the plant; the improved traits include: improving the disease resistance of the plant. The present application also provides the use of RL1 as a molecular marker for identifying plant disease resistance, or as a molecular marker for targeted screening of plants with disease resistance, and related identification methods, screening methods, etc.

This application pertains to the fields of plant growth regulation and fertilizers, and provides a fertilizer for plant gene activation. The fertilizer comprises modified humic acid, chitosan, salicylic acid, an emulsifier, and water. This application modifies humic acid through a combination of sulfonation and pyrolysis, further enhancing the growth-promoting effect of the fertilizer, particularly significantly increasing the expression levels of key root growth genes.

Recombinant DNA molecules and constructs are provided that are useful for modulating gene expression in plants. One or more expression cassette(s) of a recombinant DNA molecule or construct may be excised from transgenic plants following transformation by the presence of flanking site-specific recombination sites in the recombinant DNA molecule or construct by expression of a recombinase enzyme encoded by the recombinant DNA molecule or construct. Such a recombinase system may be used to remove such expression cassette(s) from plants transformed with the recombinant DNA construct or vector. The recombinase transgene may be operably linked to a tissue-preferred or tissue-specific promoter for autoexcision in transformed plants without crossing to a different transgenic line expressing the recombinase. Methods for causing autoexcision of one or more expression cassette(s) in a transgenic plant, and plants and cells containing or transformed with a recombinant DNA molecule or construct of the present disclosure, are also provided.

This invention discloses the application of the PhZPT2-5 gene in regulating anthocyanin synthesis in petunias, belonging to the field of plant genetic engineering technology. This invention discovers that the PhZPT2-5 gene is significantly correlated with anthocyanin synthesis in petunias. Experiments show that silencing the PhZPT2-5 gene reduces anthocyanin synthesis, while overexpressing the PhZPT2-5 gene promotes anthocyanin synthesis. By regulating the expression of the PhZPT2-5 gene in petunias, different colored petunias can be obtained, laying the foundation for breeding new petunia varieties.

This utility model discloses an apparatus for plant genetic transformation culture, comprising a culture dish and culture bottle caps used in combination. The side of the culture dish is provided with graduations, including a minimum graduation for subculture, an intermediate graduation for differentiation culture, and a maximum graduation for rooting culture. The culture bottle caps include subculture bottle caps, differentiation culture bottle caps, and rooting culture bottle caps, each with ventilation holes at the top. This apparatus allows for precise control of the culture medium volume, avoiding waste and effectively reducing culture costs; it also improves the versatility of the apparatus, expanding its application range; and it facilitates observation and gas exchange, promoting healthy plant growth.

This invention belongs to the field of plant gene editing technology, specifically relating to a bifunctional gene editing system and its application in single-parent maize. The invention provides a bifunctional gene editing system comprising: a fusion protein expression cassette and a guide RNA combined expression cassette; wherein the guide RNA combined expression cassette includes: pegRNA associated with the maize D9 gene; and sgRNA associated with the maize YUC4 and YUC2 genes. Transient expression validation using the bifunctional gene editing system in maize protoplasts revealed that the system achieves a precise editing efficiency of 3.5% at the G571V site of the D9 gene, a knockout efficiency of 40%-65% for the YUC4 gene, and a knockout efficiency of 37%-54% for the YUC2 gene. This demonstrates that the technical solution of this application can simultaneously and efficiently achieve precise point mutation and gene knockout within the same cell, thus enabling its application in the synergistic improvement of lodging resistance in maize.

This invention belongs to the fields of genetic engineering and enzyme engineering, specifically relating to a sucrose synthase mutant, its preparation method, product, and application. The mutant is based on the amino acid sequence of the wild-type sucrose synthase shown in SEQ ID NO.1, with the 94th amino acid mutated from P to N, resulting in the amino acid sequence shown in SEQ ID NO.2; or the 660th amino acid mutated from Y to H, resulting in the amino acid sequence shown in SEQ ID NO.3. This mutant can be used for in vitro glycosylation reactions, plant genetic improvement, or industrial biocatalysis, solving the technical problems of poor stability and low catalytic efficiency of natural enzymes.

The present invention relates to the field of promoter activation to increase expression of a target gene in a plant. More specifically, the present invention provides a method for increasing the expression level of an endogenous polynucleotide of interest in a genomic locus of a plant, wherein the native promoter is screened for a suitable motif, which can, preferably by a single point mutation, be modified to increase the expression of the endogenous polynucleotide of interest and then the mutation is introduced, preferably by TILLING. The invention also provides a method of producing a modified promoter, a modified promoter as well as a method of screening a promoter endogenous to a plant for a motif, which can be modified by introducing one or more point mutations to create a motif, which increases the expression of the polynucleotide under the control of the promoter with respect to the unmodified promoter.

This invention discloses a tea plant liquid phase chip and its applications, belonging to the field of molecular detection technology. The invention discloses a tea plant liquid phase chip and its applications, which, based on site screening requirements and probe design principles, includes 5781 SNP sites. It can achieve genotyping of tea plant resources using precise localization sequencing and genotyping technology based on liquid phase capture of target genomic sequences. This tea plant liquid phase chip of the invention enables low-cost genotyping, primarily due to its tea plant specificity. It can facilitate tea plant variety identification and phylogenetic analysis, scientifically guide tea plant hybridization and improvement work, contribute to the protection and development of tea plant germplasm resources, and has high application value in multiple fields of tea plant breeding. The tea plant liquid phase chip of the invention can be used for tea plant genetic diversity assessment, germplasm resource and phylogenetic identification, genetic map construction and gene localization, genome-wide association analysis, and marker-assisted breeding of tea plants.

The application discloses an anti-disease related protein OsMED16, a biomaterial thereof and application of the protein. The application solves the technical problem of improving plant disease resistance, reducing plant tillering number, delaying plant flowering time or preparing a plant with delayed flowering time. The application specifically provides a protein and a biomaterial thereof. The protein is any one of the following: A1) a protein with an amino acid sequence shown in sequence 2; A2) a protein with more than 80% identity with the protein shown in A1) and capable of improving plant disease resistance, which is obtained by substitution, deletion and / or addition of amino acid residues of the protein in A1); and A3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1) or A2). Replacing a coding gene of the protein in a related gene in a plant genome can improve plant disease resistance, reduce plant tillering number and delay plant flowering time, and is used for rice variety improvement or breeding.

This invention belongs to the field of plant gene map-based cloning and molecular breeding, and relates to the application of the maize gene BRR1 and its functional site in regulating harvest index and yield traits. Through QTL mapping and fine mapping of a maize-Kesury grass population, this invention identified the major-effect QTL-HI1 regulating the harvest index and its key gene BRR1, and discovered that its upstream 9.1 kb insert is an enhancer with H3K27ac modification, which can physically interact with the BRR1 promoter and significantly upregulate gene expression. Knockout of BRR1 leads to a decrease in harvest index, biomass, and yield, and delayed flowering; overexpression of BRR1 increases harvest index and yield by 10–16%. This gene and its functional site are subject to strong selection during domestication and breeding, and can be used through transgenic technology or marker-assisted selection to achieve synergistic improvement of maize harvest index, yield, and flowering time, providing a new approach for high-yield molecular design breeding.

Provided is a simple and efficient method of plant genome editing that does not involve the incorporation of a gene cassette for the expression system of a site-specific DNA modification protein. The method of the present invention comprises: (i) a step for mixing plant cells or a tissue containing plant cells together with a site-specific DNA modification protein and a needle-shaped inorganic compound in a liquid medium and making a disturbance to perforate the cells with the needle-shaped inorganic compound, thereby introducing the site-specific DNA modification protein into the cells; and (ii) a step for culturing the plant cells or the tissue containing the plant cells, into which the site-specific DNA modification protein has been introduced in step (i), thereby causing a DNA mutation specific to a target site in the genome of the plant cells.

The present invention relates to a method for producing a MAGEA4-Fc transgenic plant, and can stably express the recombinant protein MAGEA4-Fc in which a human IgG Fc fragment is fused to a MAGEA4 protein through a platform using a tobacco plant. The method according to the present invention enables high-level expression efficiency and stable protein production by introducing a target gene into a plant genome by using an Agrobacterium binary vector. Mass production can be easily achieved by directly separating and purifying the recombinant protein from the transformed tobacco plant, and greater economic feasibility and expandability are provided compared to existing microbial or animal cell-based expression systems, thus providing greater utility as a fusion protein production platform.

The present application relates to the field of agricultural ecological monitoring and molecular diagnosis, and specifically discloses a method for rapidly detecting soil cadmium pollution by using plant genetic markers, which aims to solve the problems of complex pretreatment, dependence on large instruments and inability to reflect biological availability in existing soil cadmium detection, selects the cadmium-specific response gene rb_592991 of the super-hyperaccumulator Bidens pilosa as a target, extracts the total RNA of the Bidens pilosa exposed to the soil to be detected, performs one-pot constant-temperature reaction at 60 DEG C by using RT-LAMP combined with CRISPR / Cas12b system, and realizes naked-eye visual interpretation by using a colloidal gold lateral flow test strip. The detection system of the present application has a minimum detection limit (LOD) of 0.2 mg / L in a water culture environment, has strong anti-interference property, does not require expensive instruments, and can rapidly evaluate the biological availability of soil cadmium in situ, thereby providing a simple, efficient and new strategy for early warning of cadmium pollution in farmland and traditional Chinese medicine production areas.

The invention discloses a Clematis florida ClLEA coding sequence and application thereof, and belongs to the fields of molecular biology, plant physiology, gene engineering and the like. According to the invention, a ClLEA coding sequence is screened and expressed in clematis, so that the water logging tolerance of arabidopsis thaliana is remarkably improved. The gene and the protein thereof provided by the invention can be applied to genetic improvement of plants, and an effective technical means is provided for cultivating new varieties of crops, horticultural crops and the like with enhanced waterlogging tolerance.

The present application belongs to the field of plant genetic breeding and genetic engineering technology, and particularly relates to a breeding method for rapid transfer of excellent genes of polyploid rice. The present application uses a rice haploid induction gene OsMATL to edit in a new type of tetraploid rice, and create a knock-out mutant of the OsMATL gene. Then, the knock-out mutant of the OsMATL gene is used as a male parent to cross with different types of homologous tetraploid rice female parents, and further create a "new" diploid rice carrying excellent genes of tetraploid rice. The rice material carries excellent alleles of tetraploid rice, and can be directly used in existing rice breeding. The method of the present application can quickly transfer excellent variations in tetraploid rice, and be applied to existing rice breeding systems.

The present invention relates to a method for producing a MAGEA3-Fc transgenic plant, and enables, through a platform using a tobacco plant, stable expression of a recombinant protein MAGEA3-Fc in which a human IgG Fc fragment is fused to a MAGEA3 protein. The method of the present invention enables high-level expression efficiency and stable protein production by using an Agrobacterium binary vector to introduce a target gene into a plant genome. The present invention enables recombinant proteins to be readily produced on a large scale through direct isolation and purification from transgenic tobacco plants, and is more economical and scalable than conventional microbial or animal cell-based expression systems, and thus can be effectively used as a fusion protein production platform.

The application belongs to the technical field of plant gene editing, and particularly relates to a plant genome editing system based on glycosylase MPG_V6.3 and application thereof. The application aims to develop a genome editing tool with high-efficiency base conversion and polynucleotide deletion. The technical scheme of the application is a coding gene of glycosylase MPG_V6.3, which has a nucleotide sequence as shown in Seq ID No. 1, 2065-2955. The application further provides a plant genome editing system based on the glycosylase MPG_V6.3, which comprises an MPG_V6.3-nCas9 fusion expression unit with a structure of promoter-MPG_V6.3-nCas9-terminator. The editing system realizes a leap from "single base editing" to "integrated editing of base editing and multi-base deletion", and can simultaneously realize base type conversion and multi-base deletion at a target site.

This invention discloses a tea plant CsFEZ gene, its encoded protein, and its applications, specifically relating to the field of tea plant breeding technology. The CDS sequence of the tea plant CsFEZ gene is shown in SEQ ID No: 1, and the amino acid sequence of its encoded protein is shown in SEQ ID No: 2. By controlling the expression of the CsFEZ gene and its encoded protein in tea and rice somatic embryos, the occurrence of somatic embryos in tea and rice can be effectively regulated to obtain somatic embryos with high regeneration efficiency. This can provide a new solution for the genetic transformation of varieties with low regeneration efficiency. Furthermore, the CsFEZ gene can serve as an important molecular mechanism in the process of plant somatic embryo regeneration, providing a foundation for research and application in plant gene synthesis and customized plant modification.

This invention relates to the field of genetic engineering. Specifically, it relates to cytosine deaminases and their use in base editing. More specifically, it relates to a method for screening and identifying deaminases, a base editing system based on newly identified cytosine deaminases, a method for base editing a target sequence in the genome of an organism (e.g., a plant) using this base editing system, and genetically modified organisms (e.g., plants) and their offspring produced by said method.

This invention relates to the application of the microprotein gene ZmHLH1 in regulating primary root elongation in plants, belonging to the field of plant gene breeding technology. The nucleotide sequence of the microprotein gene ZmHLH1 is shown in SEQ ID NO.1, and the amino acid sequence of the protein encoded by this gene is shown in SEQ ID NO.2. Overexpression of the ZmHLH1 gene promotes primary root elongation and improves seedling quality. This invention is the first to discover that overexpression of the ZmHLH1 gene can promote primary root growth in maize, providing a new functional element and theoretical basis for breeding superior maize varieties with robust primary roots and uniform seedlings.

The application discloses a use of overexpression of an OsYIG gene in improving rice yield and belongs to the field of genetic engineering and plant genetic breeding. It is found that the expression level of the OsYIG gene is improved in rice, and the yield of the rice is improved without affecting the chalkiness and chalky grain rate of the rice.

The utility model discloses a kind of super-clean bench for facilitating washing callus when plant genetic transformation, belong to super-clean bench technical field, it includes operation platform, the operation platform top is separately fixedly connected with protective cover and a pair of guide rail, oscillation subassembly is arranged on the protective cover.The utility model is provided with oscillation subassembly, rack, cover plate, locking block and locking assembly, place the culture dish with washing solution and callus in the placing groove on rack, rotate cover plate, locking assembly locks cover plate through locking block in the side of cover plate, so that cover plate firmly presses the dish cover of culture dish, oscillation subassembly can drive rack to shake back and forth, simultaneously handle multiple culture dishes, setting has take and place component, after washing, take and place component can simultaneously eject multiple culture dishes from the placing groove on rack through lifting disc, facilitate experimental personnel to take out culture dish, save experimental time, improve experimental efficiency.

This invention discloses a miR204 derived from *Mustela stenoptera* and its applications. The mature sequence of miR204 is shown in SEQ ID NO. 1, and its precursor sequence is shown in SEQ ID NO. 2. This invention is the first to discover *Mustela stenoptera* miR204 and demonstrates through molecular genetic methods that this miRNA not only affects plant resistance to drought but also has a dual function of regulating plant salt tolerance. This research not only enriches the plant stress resistance gene pool and provides a key target gene for plant genetic improvement but also offers a new option for breeding high-quality stress-resistant varieties. It also provides reliable materials and data support for research on the molecular biological mechanisms of enhancing the stress resistance of *Mustela stenoptera*.

The application belongs to the technical field of gene editing, and particularly relates to a Cas9 protein mutant and application thereof in plant gene editing. The application discloses a Cas9 protein mutant, an amino acid sequence of which is shown as SEQ ID NO. 1. The Cas9 protein mutant can efficiently screen cutting of various target sites, and is a Cas9 protein not dependent on NGG target site cutting. The application not only widens the targeting range of the Cas9 protein, but also provides the Cas9 protein mutant with higher gene editing efficiency, and has important application value in plant genome editing.