596 results about "Plant genes" patented technology

The present invention relates to the fields of genetic engineering and plant genetic breeding, and specifically to the application of wheat TaPND gene. In the present invention, by taking gene TaPND as a target, designing a sgRNA sequence based on CRISPR / Cas9, connecting a DNA fragment containing the encoding sgRNA sequence to a vector carrying CRISPR / Cas, a plant binary expression vector capable of knocking out the TaPND gene in wheat is constructed; by genetically transforming wheat, offspring with TaPND gene knockout are obtained, and wheat mutants with early flowering time are obtained. Experiments have confirmed that transgenic wheat with TaPND gene knockout achieves the advancement of wheat flowering time. The technical scheme of the present invention provides a practical method for realizing rapid wheat breeding using genetic engineering technology, which has important breeding application value and broad market application prospects.

Provided are compositions for genome editing and site-directed integration in plants comprising microprojectile particles coated, treated of applied with a recombinant DNA construct comprising a sequence encoding one or more genome editing reagents for delivery to a mature embryo explant from dry seeds. Further provided are methods for genome editing and site-directed integration in at least one cell of a plant using the disclosed compositions, and plants, plant parts and seeds comprising an edited genome or site-directed integration, which are produced by the disclosed methods.

The invention relates to the technical field of collaborative prediction, in particular to a plant polygene stress resistance collaborative prediction method and system based on a federal map neural network. The method comprises the following steps: modeling a multilayer heterogeneous graph according to acquired plant genome data; constructing a dynamic graph neural network model based on time sequence perception; training a dynamic graph neural network model by using the multi-layer heterogeneous graph, and performing distributed privacy calculation on the multi-layer heterogeneous graph by using a federated graph neural network; generalization training is carried out on the small sample scene data based on multi-task learning and a cross-species migration mechanism; and obtaining a gene function prediction result. Through a multi-layer heterogeneous graph fusion technology and a dynamic graph neural network design of time sequence perception, multi-dimensional biological information such as gene regulation, protein interaction, metabolic pathways and stress response can be captured at the same time, and time sequence change characteristics of gene expression in the plant stress response process can be captured at the same time.

The invention relates to the field of plant gene map-based cloning and molecular breeding. Specifically, the invention provides a nucleic acid molecule and a polypeptide, and also provides a recombinant vector, a host cell and a plant or a part, a seed, a cell or a progeny thereof containing the nucleic acid molecule. Furthermore, the invention also provides a method for preparing the transgenic plant, and a method for regulating and controlling the nitrogen utilization efficiency, nitrate absorption or transport, biomass and / or yield of the plant.

The invention belongs to the technical field of plant gene engineering, and particularly relates to an OsNUC1 gene and application of a gene mutant constructed by the OsNUC1 gene to regulation and control of rice plant height, leaf length and panicle type. The regulation is to regulate the plant height and leaf growth of the rice and influence the shape of the ear part. Compared with the prior art, the OsNUC1 gene mutant has the following advantages: (1) the OsNUC1 is directionally edited by utilizing a CRISPR / Cas9 gene editing technology, an OsNUC1 gene mutant rice material is obtained, the biological phenotype of the OsNUC1 gene mutant rice material is investigated, the phenotype investigation on T1-generation seedlings of the OsNUC1 shows that the plant heights and the leaf lengths of one-week-old and two-week-old seedlings of the mutant are obviously higher than those of wild seedlings, the leaf widths have no obvious difference, and the OsNUC1 gene mutant rice material has a good application prospect; the result shows that the OsNUC1 can regulate the plant height and the leaf growth of the rice and influence the shape of the ear part. (2) it is proved that the nucleolin gene OsNUC1 is negatively regulated by a transcription factor OsARF7, and the phenotypes such as the plant height, the leaf length, the grain length and the grain width of the OsNUC1 / OsARF7 mutant are more obvious than those of the OsNUC1 mutant. The invention further proves the application of the OsNUC1 gene in regulation and control of rice plant height and leaf growth.

The present invention relates to the field of genetic engineering. Specifically, the present invention relates to cytosine deaminase and use thereof in base editing. More specifically, the present invention relates to a base editing system based on a newly identified cytosine deaminase, a method for base editing a target sequence in the genome of an organism (e.g., a plant) using the base editing system, and a genetically modified organism (e.g., a plant) produced by the method and progenies thereof.

The invention discloses application of arabinogalactan protein family genes PbrAGP16 in promoting growth of pear pollen tubes, and belongs to the technical field of plant genetic engineering. According to the invention, the gene PbrAGP16 is cloned from pollen of Dangshan pears by using a plant gene cloning technology, and the nucleotide sequence of the gene PbrAGP16 is shown as SEQ ID No.1; the amino acid sequence coded by the gene is SEQ ID No.2 in a sequence table. Researches show that PbrAGP16 can promote growth of pear pollen tubes, the change of PbrAGP16 expression is related to the change of the content of cellulose at the top ends of the pollen tubes, and PbrAGP16 also participates in a regulation mechanism of a self-incompatible reaction. The pollen magnetic transfection technology is adopted to research the function of the gene in the pollen tube, and a wide application prospect is provided for improving the pollination efficiency.

The invention provides a structure, a sequence and a construction method of a plant virus gene editing vector and application of the plant virus gene editing vector in plant gene editing. The method is characterized in that a TRV2 genome of a tobacco embrittlement virus (TRV) is used for carrying and expressing a small Cas gene AsCas12f and gRNA of the small Cas gene AsCas12f. Preferably, the AsCas12f and the gRNA of the AsCas12f are subjected to tandem expression by utilizing the TRV2 to form an expression cassette structure of the AsCas12f-tRNA-gRNA, the expression cassette substitutes a part of 2b gene sequence of the TRV2 on a TRV2 genome to form a plant gene editing vector pTRV2-Cas12f-gRNA based on the TRV virus, and long-time stable expression and gene editing can be performed after wild type nicotiana benthamiana is inoculated. The invention also provides a method for carrying out gene editing in a wild type plant by utilizing the plant virus.

The invention discloses an application of a gene GmAGO5 of a soybean ARGONAUTE family. The gene GmAGO5 of the soybean ARGONAUTE family is shown The nucleotide sequence of the soybean GmAGO5 protein coding gene GmAGO5 is as shown in SEQ ID NO. 1. The invention also discloses a preparation method of the soybean GmAGO5 protein coding gene The constructed plant gene editing knockout vector pSCM-GmAGO5 is transferred into an acceptor material Tianlong No.1 by using a cotyledonary node transformation method. The GmAGO5 gene is knocked out, so that the plant height of the gene edited soybean is reduced, the branch included angle is reduced, and the protein content of the soybean seeds is remarkably increased. Therefore, the soybean GmAGO5 protein coding gene GmAGO5 disclosed by the invention can be applied to reducing soybean plant height, reducing branch included angle and improving the protein content of soybean seeds through a genetic engineering means.

The invention belongs to the technical field of plant heredity and genetic engineering, and particularly relates to a wheat saline-alkaline tolerant protein kinase gene TaCRCK and application thereof. Specifically, the invention discloses a wheat saline-alkaline tolerant protein kinase gene TaCRCK, and the nucleotide sequence of cDNA of the gene is as shown in SEQ ID NO. 1. The invention also discloses a plant expression vector containing the gene TaCRCK and application of the gene and the plant expression vector in cultivation of saline-alkali resistant plants. Tests prove that silent expression of the gene promotes the resistance of wheat to saline-alkaline stress, and the saline-alkaline tolerance of transgenic plants is obviously improved. The gene has a negative regulation effect on the salt and alkali tolerance of plants, provides a new tool and method for improving the salt tolerance of crops by using a genetic engineering means, and has important scientific significance and application prospects.

The invention belongs to the technical field of plant heredity and genetic engineering, and particularly relates to a wheat saline-alkaline tolerant ERF transcription factor gene TaERF109 and application thereof. The invention discloses the wheat saline-alkali stress response gene TaERF109 obtained by cloning by utilizing the existing plant gene engineering technology for the first time, the gene is overexpressed in wheat through an agrobacterium tumefaciens mediated method, and comparative analysis proves that the saline-alkali tolerance of a transgenic plant is obviously improved, and the transgenic plant has a good application prospect. And interference on expression of the TaERF109 can reduce the resistance of the wheat to salt and alkali stress. Therefore, the invention fully proves that the wheat saline-alkaline tolerant ERF transcription factor gene plays an important role in plant saline-alkaline tolerant regulation and control, and shows that the gene can be widely applied to cultivation and improvement of plant saline-alkaline tolerant varieties, so that the wheat saline-alkaline tolerant ERF transcription factor gene has good practical application value.

The invention provides a method for realizing fixed-point activation of a plant genome by directionally inserting an enhancer, and particularly, by inserting a short enhancer (STE) in a specific region of a target gene promoter, a target gene can be efficiently activated, the expression level of the target gene can be enhanced by about 2000 times, and the enhancement multiple can be flexibly adjusted as required. The site-specific activation method for the plant genome has high efficiency, wide applicability and stable heredity, and has outstanding application value in plant research and breeding.

The invention relates to the technical field of plant genetic breeding, in particular to a rice sheath blight resistance related gene and an encoding protein and application thereof. The invention provides a rice sheath blight resistance related gene as well as an encoding protein and application thereof, the gene is OsSBR4 or a related gene thereof, the resistance of rice to sheath blight can be remarkably enhanced by knocking out the gene, and the resistance of rice to sheath blight can be reduced by overexpressing the gene. After agronomic traits of a transgenic line of the gene are investigated, it is found that the plant height, the ear length, the ear number, the thousand seed weight and the like are not affected, and it is indicated that the gene has important application value in sheath blight resistance molecular breeding. According to the invention, a novel rice sheath blight resistance related gene is screened and excavated, and the identification of the sheath blight resistance gene can provide a theory and a basis for breeding a sheath blight resistance rice variety.

The invention discloses application of a pear transcription factor PbrWHY2 gene in promoting growth of pear pollen tubes, and belongs to the technical field of plant genetic engineering. According to the invention, the gene PbrWHY2 is cloned from pollen of Dangshan pears by using a plant gene cloning technology, and the nucleotide sequence of the gene PbrWHY2 is shown as SEQ ID No.1; the amino acid sequence coded by the gene is SEQ ID No.2 in a sequence table. The PbrWHY2 can promote the growth of a pear pollen tube, the change of the expression of the PbrWHY2 is related to the change of ROS level and cellulose content at the top end of the pollen tube, and meanwhile, the PbrWHY2 also participates in a regulation mechanism of a self-incompatible reaction to regulate a downstream gene PbrAGP16 / PbrRALF6 / PbrGIS1 in a targeted manner. The pollen magnetic transfection technology is adopted to research the function of the gene in the pollen tube, and a wide application prospect is provided for improving the pollination efficiency.

The invention relates to the field of gene engineering. In particular, the present invention relates to cytosine deaminases and their use in base editing. More specifically, the invention relates to a method for screening and identifying a deaminase, a base editing system based on a newly identified cytosine deaminase, a method for editing a target sequence in a genome of an organism (such as a plant) by using the base editing system, and a method for screening and identifying the target sequence. As well as genetically modified organisms (e.g., plants) and progeny thereof produced by the method.

The invention discloses an efficient genetic transformation method of stem tumor sesbania, and belongs to the technical field of plant genetic transformation. According to the efficient genetic transformation method of the stem tumor sesbania, the cotyledonary node is used as the initial explant, callus induction is not needed, an optimized culture medium component is combined, a genetic transformation system of the stem tumor sesbania can be rapidly and effectively established, the genetic transformation efficiency can be improved and can reach 25% or above, the transformation time is shortened, and the cost is reduced. And a foundation is laid for domestication and accurate improvement work of the stem tumor sesbania.

The invention discloses a TaIMPA1 gene and application of the TaIMPA1 gene in improvement of plant genetic transformation efficiency. The nucleotide sequence of the gene is shown as SEQ ID No: 1 in a sequence table. According to the invention, the key gene TaIMPA1 is cloned from a plant body to delivery for the first time, and the genetic transformation performance is enhanced by remarkably improving the delivery efficiency of exogenous DNA (Deoxyribose Nucleic Acid). The discovery fills up the research blank of plant endogenous delivery regulatory factors, and an original solution is provided for overcoming the core problem of genotype dependence.

The invention discloses an RNA (Ribonucleic Acid) aptamer and application thereof in improving the salt tolerance of rice. The aptamer sequence eApt-2 with high affinity to eIF4A is obtained through enrichment by using a phylogenetic screening technology of the nucleic acid aptamer, and the aptamer sequence eApt-2 can inhibit the RNA helicase activity of eIF4A and selectively block a 5'cap-dependent translation initial process. After the eApt-2 coding sequence is introduced into a rice cell, the transgenic rice shows significantly enhanced salt tolerance under a salt stress condition: the growth condition is improved, and the biomass and the photosynthetic efficiency are both higher than those of a control plant. Compared with a traditional strategy of improving the salt tolerance through gene overexpression or knockout, the method does not need permanent modification of animal and plant genomes, accurate and reversible regulation and control of translation initiation factors are carried out through aptamers, optimization of stress response protein synthesis is achieved, and higher specificity and safety are achieved. The invention provides a novel and efficient technical approach for cultivating salt-tolerant crops, and has a wide application prospect.

Provided are compositions for genome editing and site-directed integration in plants comprising microprojectile particles coated, treated or applied with a nuclease protein, guide RNA or RNP for delivery to a mature embryo explant from dry seeds. Further provided are methods for genome editing and site-directed integration in at least one cell of a plant using the disclosed compositions, and plants, plant parts and seeds comprising an edited genome or site-directed integration, which are produced by the disclosed methods.

The invention discloses a method for site-specific replacement or insertion of DNA fragments in a plant genome, and belongs to the technical field of biology. The method disclosed by the invention comprises the following steps: editing a genome of a plant protoplast by adopting a CRISPR / Cas9 system and taking a double-stranded DNA repair template as a donor template to obtain an edited protoplast; and carrying out culture regeneration on the edited protoplast by utilizing a plant protoplast transformation method to obtain a site-directed mutagenesis plant in the genome so as to realize site-directed mutagenesis of the target plant genome. The method disclosed by the invention can be used for accurately replacing DNA in a plant genome at a fixed point. According to the invention, a recognition sequence of recombinase can be accurately inserted into a genome at a fixed point through homologous recombination, and then accurate fixed-point replacement of a gene large fragment is realized by virtue of a specific recombinase system.

The invention relates to application of a corn ZmECT3 gene in regulation and control of plant heat resistance, and belongs to the technical field of plant gene breeding, the nucleotide sequence of the ZmECT3 gene is as shown in SEQ ID NO.1, and the amino acid sequence of the gene encoding protein is as shown in SEQ ID NO.2. By analyzing a ZmECT3 gene deleted corn plant and a ZmECT3 gene overexpressed arabidopsis thaliana plant, it is found that the ZmECT3 gene can regulate and control the heat resistance of the plants, and important theoretical significance and application value are provided for creation of heat-resistant corn new germplasm and heat-resistant corn molecular breeding.

The invention relates to the field of plant gene map-based cloning and molecular breeding. Specifically, the invention provides a nucleic acid molecule and a polypeptide, and also provides a recombinant vector, a host cell and a plant or a part, a seed, a cell or a progeny thereof containing the nucleic acid molecule. Furthermore, the invention also provides a method for preparing the transgenic plant, and a method for regulating and controlling the nitrogen utilization efficiency, nitrate absorption or transport, biomass and / or yield of the plant.

In traditional plant breeding approaches, chemical mutagenesis may be utilized to introduce nucleotide substitutions at random in the genome of a plant, i.e. without possibilities to control the sites of nucleotide changes. Because of genome complexities, the statistical probability is extremely little when it comes to finding a predetermined nucleotide substitution. The present invention, however, demonstrates how a novel, alternative use of digital polymerase chain reaction (dPCR), preferably droplet dPCR (ddPCR), is developed to exploit finding of specific nucleotide substitutions in mutated genes. The entire platform comprises a screening method with a library of mutagenized organisms, digital PCR-based systems and a set-up to propagate and analyze identified, mutated organisms.

The invention relates to a gene ZmILR1 for controlling the size of a corn kernel as well as a molecular identification method and application of the gene ZmILR1, and belongs to the technical field of plant genetic breeding and functional gene research. The ZmILR1 gene encodes an IAA-amino acid hydrolase, mutation of the IAA-amino acid hydrolase causes corn kernels to become smaller, and overexpression of the gene can create a corn material with increased kernel length, kernel width and hundred-kernel weight. A functional molecular marker A1A4 is developed according to the sequence difference of the mutant gene and a wild type gene at the DNA level, and can be used for rapid identification and screening of small-grain corn materials. The invention provides a theoretical basis and an application tool for analyzing a molecular mechanism of the corn grain size and developing molecular marker-assisted breeding and hybrid seed breeding, and provides important gene resources and technical support for high-yield and high-quality molecular breeding of corn.

The invention belongs to the field of gene engineering, and relates to a plant genome directional editing tool based on transposon-coded nuclease. The invention aims to solve the technical problem that the application range of a plant genome editing tool based on CRISPR-Cas9 and Cas12a is limited to a great extent due to the fact that CRISPR-Cas9 and Cas12a are large in protein size and difficult to deliver. The invention provides transposon nuclease suitable for plant genome editing. The transposon nuclease is IsDge10, IsAam1 or enIscB subjected to codon preference optimization; and a gene editing system containing the three transposon nuclease is constructed. The gene editing system can be suitable for editing a coding region, a non-coding region and the like; the method can be widely applied to monocotyledonous plants, dicotyledonous plants, gymnosperm and the like.

This invention discloses a rice starch synthesis-related gene, OsFLO17, its encoded protein, and its applications. Through phenotypic analysis of the rice endosperm mealy mutant flo17 and preliminary localization of the target gene, the starch synthesis-related protein OsFLO17 was ultimately cloned. This protein consists of the amino acid sequence shown in SEQ ID NO.3. The starch granule development-related protein of this invention affects the rice endosperm starch synthesis process. Introducing the encoding gene of this protein into plants with abnormal starch granules can yield transgenic plants with normal starch filling. Therefore, the protein and its encoded gene of this invention can be applied to plant genetic improvement.

The invention discloses a plant gene function analysis method (FAGEN), and belongs to the technical field of molecular genetics and functional genomics. The method comprises the following steps: constructing a target gene mutant by using a gene editing technology; hybridizing the mutant and the corresponding wild type as a common parent with a plurality of parents with different genetic backgrounds to construct a pair F1 group; an F2 group is obtained through selfing; and determining phenotypic data of target characters of F1 and F2 populations, and comparing phenotypic differences of filial generations of wild types and mutants under different genetic backgrounds to judge the functions of the target genes. The method has the advantages of simplicity in operation, flexibility in design, short construction period and low cost, and gene functions can be efficiently and accurately analyzed under various genetic backgrounds.

The invention belongs to the technical field of plant genetic transformation, and particularly relates to a method for rapidly obtaining a citrus rootstock genetic transformation root system and application thereof. According to the genetic transformation method, agrobacterium rhizogenes K599 is used as a medium, an infection solution is transfected through a wound of a citrus plant, the infection solution can be quickly transfected into a stem segment of the citrus plant, and finally, the genetically transformed citrus plant can be quickly obtained in a high-pressure propagation mode. The method provided by the invention can effectively shorten the transformation system period, reduce the genetic transformation cost, improve the genetic transformation efficiency and avoid the generation of chimeras, and the materials can be repeatedly utilized.

The invention belongs to the technical field of plant gene editing, and particularly relates to application of a cotton GhWRKY46 gene in regulation and control of cotton fiber quality. The invention provides an application of a GhWRKY46 gene in regulation and control of cotton fiber quality. An amino acid sequence coded by the GhWRKY46 gene is as shown in SEQ ID NO: 1. According to the method, the endogenous GhWRKY46 gene of upland cotton is precisely edited, so that the fiber length of the obtained gene edited cotton is increased, the thickness of a secondary wall is reduced, the micronaire value is reduced, the specific breaking strength is improved, the cotton fiber quality is remarkably improved, and a cotton excellent germplasm resource with the fiber length and strength improved is obtained. According to the gene provided by the invention, the comprehensive quality of cotton fibers is remarkably superior to that of wild cotton fibers, and the gene has important agricultural application value.

The present invention provides a method for improving plant genetic transformation and gene editing efficiency. Specifically, the method improves the regeneration efficiency of plant genetic transformation and / or improves the efficiency of plant gene editing by expressing genes that promote plant cell division, especially meristematic cell division.