352 results about "Plant genes" patented technology

The invention relates to the field of gene engineering. In particular, the present invention relates to cytosine deaminases and their use in base editing. More specifically, the invention relates to a method for screening and identifying a deaminase, a base editing system based on a newly identified cytosine deaminase, a method for editing a target sequence in a genome of an organism (such as a plant) by using the base editing system, and a method for screening and identifying the target sequence. As well as genetically modified organisms (e.g., plants) and progeny thereof produced by the method.

Provided are compositions for genome editing and site-directed integration in plants comprising microprojectile particles coated, treated or applied with a nuclease protein, guide RNA or RNP for delivery to a mature embryo explant from dry seeds. Further provided are methods for genome editing and site-directed integration in at least one cell of a plant using the disclosed compositions, and plants, plant parts and seeds comprising an edited genome or site-directed integration, which are produced by the disclosed methods.

The invention relates to a gene ZmILR1 for controlling the size of a corn kernel as well as a molecular identification method and application of the gene ZmILR1, and belongs to the technical field of plant genetic breeding and functional gene research. The ZmILR1 gene encodes an IAA-amino acid hydrolase, mutation of the IAA-amino acid hydrolase causes corn kernels to become smaller, and overexpression of the gene can create a corn material with increased kernel length, kernel width and hundred-kernel weight. A functional molecular marker A1A4 is developed according to the sequence difference of the mutant gene and a wild type gene at the DNA level, and can be used for rapid identification and screening of small-grain corn materials. The invention provides a theoretical basis and an application tool for analyzing a molecular mechanism of the corn grain size and developing molecular marker-assisted breeding and hybrid seed breeding, and provides important gene resources and technical support for high-yield and high-quality molecular breeding of corn.

This invention discloses a rice starch synthesis-related gene, OsFLO17, its encoded protein, and its applications. Through phenotypic analysis of the rice endosperm mealy mutant flo17 and preliminary localization of the target gene, the starch synthesis-related protein OsFLO17 was ultimately cloned. This protein consists of the amino acid sequence shown in SEQ ID NO.3. The starch granule development-related protein of this invention affects the rice endosperm starch synthesis process. Introducing the encoding gene of this protein into plants with abnormal starch granules can yield transgenic plants with normal starch filling. Therefore, the protein and its encoded gene of this invention can be applied to plant genetic improvement.

The invention discloses a plant gene function analysis method (FAGEN), and belongs to the technical field of molecular genetics and functional genomics. The method comprises the following steps: constructing a target gene mutant by using a gene editing technology; hybridizing the mutant and the corresponding wild type as a common parent with a plurality of parents with different genetic backgrounds to construct a pair F1 group; an F2 group is obtained through selfing; and determining phenotypic data of target characters of F1 and F2 populations, and comparing phenotypic differences of filial generations of wild types and mutants under different genetic backgrounds to judge the functions of the target genes. The method has the advantages of simplicity in operation, flexibility in design, short construction period and low cost, and gene functions can be efficiently and accurately analyzed under various genetic backgrounds.

The invention belongs to the technical field of plant gene editing, and particularly relates to application of a cotton GhWRKY46 gene in regulation and control of cotton fiber quality. The invention provides an application of a GhWRKY46 gene in regulation and control of cotton fiber quality. An amino acid sequence coded by the GhWRKY46 gene is as shown in SEQ ID NO: 1. According to the method, the endogenous GhWRKY46 gene of upland cotton is precisely edited, so that the fiber length of the obtained gene edited cotton is increased, the thickness of a secondary wall is reduced, the micronaire value is reduced, the specific breaking strength is improved, the cotton fiber quality is remarkably improved, and a cotton excellent germplasm resource with the fiber length and strength improved is obtained. According to the gene provided by the invention, the comprehensive quality of cotton fibers is remarkably superior to that of wild cotton fibers, and the gene has important agricultural application value.

The invention discloses a tool for realizing efficient specific gene inhibition in a plant by utilizing a SunTag system of CRISPR-dCas9 (clustered regularly interspaced short palindromic repeats-das9). According to the method, gRNA near a transcription start site of a targeted target gene is designed, dCas9 and scFv-GFP are accurately recruited to a target site by using a SunTag system, the transcription start process of RNA polymerase II is interfered, and the expression of the target gene is remarkably inhibited. In view of relatively high activity of the system in a plant rdr6 mutant, an artificial miR-RDR6 fragment is further introduced into a vector and is used for inhibiting expression of RDR6 in a wild plant, so that the applicability and functionality of the system are expanded. Compared with a traditional method depending on a plant endogenous effector, the method has the advantages that dependence on endogenous regulatory factors is avoided, the specificity and safety of gene inhibition are remarkably improved, and the method can be used for plant genetic function research and character improvement.

The invention discloses a plant gene editing system and method for delivering CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 based on mobile RNA (ribonucleic acid). The system does not depend on tissue regeneration or protoplast culture, Cas9 and gRNA are remotely delivered from the root to enter the overground part by fusing a CRISPR / Cas9 gene editing carrier of a tRNA-like sequence (TLS) movable motif and combining a hairy root transformation system, target gene editing is completed, and heritable gene editing seeds can be obtained at the current generation. The method comprises the following steps: 1, constructing a movable CRISPR / Cas9 gene editing fusion vector, wherein the movable CRISPR / Cas9 gene editing fusion vector comprises a target vector, an intermediate vector and an editing vector; and 2, application of the agrobacterium rhizogenes mediated plant hairy root transformation system. And 3, detecting the mobility and editing type of the movable editing carrier. And 4, gene function verification application. The system greatly breaks through the bottlenecks of difficult plant genetic transformation and long time consumption of precise breeding, and provides theoretical basis and technical support for gene function research and germplasm innovation of important agronomic traits of plants.

Provided is an efficient and precise plant gene knockout method. First, a sequence containing a stop codon cluster is inserted into a genome by means of dual pegRNA to obtain, by means of screening, a stop codon cluster sequence capable of efficiently and precisely knocking out a target gene. A prime editing system and multiple pegRNA programming can be used in combination with the method to enable various genomic modifications such as efficient and precise knockout of one or more target genes, base substitutions, and small fragment insertions, deletions, and substitutions with just one prime editing protein.

The invention discloses application of novel reverse transcriptase and related fusion protein of the novel reverse transcriptase in plant-guided editing. Candidate RT protein is obtained through systematic mining from a public database, novel reverse transcriptase cl1 with activity is screened out in combination with a fluorescence report system, and a guided editing system suitable for plants is constructed. The system tests in wheat protoplast, and verifies the editing activity of cl1 to a plurality of endogenous targets. Further combining with protein structure prediction and rational design point mutation optimization, the cl1 mutant with higher editing efficiency is obtained, and the method can be applied to accurate and effective editing of plant genomes and plant breeding and improvement.

The application discloses application of SbWOX gene and SbBBM gene in corn breeding and a corn genetic transformation method, and relates to a bioengineering technology.The application provides a nucleic acid molecule with co-expression of SbWOX protein gene and SbBBM protein gene, and corresponding gene co-expression vectors and microbial transformants.The application also provides application of the co-expression of the SbWOX gene and the SbBBM gene in the corn genetic transformation method.The application obtains corresponding genes and mutant proteins based on the SbWOX gene and the SbBBM gene of sorghum, the two genes are co-expressed in the corn genetic transformation process, even without adding plant growth hormones in the antibiotic screening medium in the tissue culture process, the plant genetic transformation process can be normally completed, and the transformation efficiency and the regeneration efficiency of the corn can be remarkably improved, the transformation rate reaches 10.42%, and the differentiation rate reaches 32.89%.

This invention relates to the field of plant genetic transformation technology, and provides a method for genetic transformation of crape myrtle stem segments with buds. The method includes: infecting crape myrtle explants with Agrobacterium tumefaciens containing the RUBY reporter gene, and co-culturing them in a co-culture medium; then transferring the co-cultured explants to a differentiation medium for differentiation culture; and inoculating the obtained clustered buds into a rooting medium to obtain genetically transformed positive seedlings; wherein the explants are crape myrtle tissue culture seedlings with stem segments containing buds. The crape myrtle stem segments used in this invention have a higher regeneration efficiency than leaves. The adventitious bud induction rate of crape myrtle leaves is 1.0%-4.7%, and the stem segment regeneration and proliferation coefficient is 5-20. A large number of positive plants can be obtained in the later stage, and no browning phenomenon occurs in any of the stem segments after infection.

The present application relates to the technical field of plant genetic breeding, and particularly relates to a Brassica campestris fatty acyl reductase BcFAR2 gene, an expression protein thereof and application, a base sequence of the Brassica campestris fatty acyl reductase BcFAR2 gene is shown as SEQ ID NO. 1, and an amino acid sequence of the expression protein is shown as SEQ ID NO. 2, the Brassica campestris fatty acyl reductase BcFAR2 gene is cloned, and BcFAR2 gene overexpression Brassica campestris sterile plants can restore pollen activity; gene knockout Brassica campestris fertile plants can significantly reduce pollen activity; in Arabidopsis thaliana, overexpression of the gene can significantly improve salt tolerance of the transgenic plants; and in Brassica campestris, overexpression of the gene can significantly enhance salt tolerance.

This invention provides a gene encoding the E3 ubiquitin ligase BnaA02.SIP1 from Brassica napus and its application, belonging to the field of bioengineering technology. The nucleotide sequence of the BnaA02.SIP1 gene described in this invention is shown in SEQ ID NO:1. Specific primers were designed based on the coding region sequence of the BnaA02.SIP1 gene from Brassica napus, and a plant genetic transformation vector overexpressing the BnaA02.SIP1 gene was constructed using the constitutive expression promoter CaMV35S. The gene was then introduced into wild-type Arabidopsis thaliana and Brassica napus using Agrobacterium-mediated transformation, resulting in transgenic Arabidopsis thaliana and Brassica napus plants. The invention also verified that this gene plays a role in regulating salt tolerance in seedlings. This invention provides a theoretical basis and gene source for cultivating new salt-tolerant rapeseed varieties.

The invention discloses a KASP molecular marker related to wheat stripe rust resistance characters and application of the KASP molecular marker, and belongs to the technical field of molecular markers and plant genetic breeding. SNP sites QYr.hzau4B of the KASP molecular marker are located at the 452, 424 and 208 basic groups of a wheat reference genome 4B chromosome, C / T mutation exists at the sites, and the nucleotide sequence of the KASP molecular marker is as shown in SEQ ID NO. 1. The KASP molecular marker can be used for identifying stripe rust resistant and susceptible varieties or strains of wheat, and a valuable molecular tool is provided for screening stripe rust resistant germplasm resources of wheat, so that the breeding efficiency of improved varieties is improved, and the breeding process is accelerated.

This invention discloses a genetic transformation and gene editing method applicable to multiple sesquiterpene species, belonging to the field of plant genetic transformation and gene editing technology. The method uses the cotyledonary nodes of sterile seedlings of the sesquiterpene genus as explants, and introduces an optimized pScEF1α-Cas9 gene editing transformation vector using Agrobacterium infection. Regenerated plants are obtained through recovery culture, selection culture, and rooting culture. The vector contains four gene expression units: pScEF1α:Cas9, pGmU6:sgRNA, pGmUBI:DsRed2, and pCaMV35S:HygR. In vivo screening is further performed using the DsRed2 fluorescent reporter gene, and the gene-edited plants are identified by molecular detection. This method is applicable to common sesquiterpene, stem-nodled sesquiterpene, and spiny sesquiterpene, and has advantages such as strong species versatility, high transformation efficiency, short cycle, visualized screening, and high positive rate, providing efficient technical support for gene function research and genetic improvement of sesquiterpene species.

The invention belongs to the field of gene engineering, and relates to nuclease generated based on AI and having a gene editing function and application of the nuclease. The technical problem to be solved by the invention is to develop more nuclease suitable for eukaryote gene editing. According to the technical scheme, nuclease AIGCprotein which is generated based on AI and has a gene editing function is obtained through the following steps that a plurality of protein amino acid sequences are generated through a known nuclease three-dimensional structure in a PDB database, the sequences are classified and compared, and a protein skeleton of the nuclease AIGCprotein is obtained through extraction; the known nuclease is Cas12. The invention provides nuclease which is generated on the basis of Protein MPNN AI and has a gene editing function, and the protein does not exist in the nature; the method can be used for constructing a plant genome editing system.

The invention relates to the technical field of plant genes, in particular to a sugarcane ScPi21 gene promoter and application thereof. The sugarcane ScPi21 gene promoter is selected from a DNA (deoxyribonucleic acid) molecule as shown in any one of the following items (1)-(4): (1) ProScPi21-ZZ1a as shown in SEQ ID NO.1; (2) ProScPi21-ZZ1b as shown in SEQ ID NO. 2; (3) ProScPi21-ZZ1c as shown in SEQ ID NO.3, and (4) ProScPi21-ZZ1c as shown in And (4) ProScPi21-ROC22 as shown in SEQ ID NO. 4. The four gene promoters obtained by the invention provide new candidate sequences for screening specific expression promoters in sugarcane genetic engineering; and a technical support is provided for sugarcane genetic engineering breeding and cultivation of excellent planting of sugarcane.

The present invention relates to a method for plant genome modification of at least one plant cell being in the developmental stage of a plant immature inflorescence meristem (IIM) cell, wherein the modification of the specific cell type is achieved by providing a genome modification or editing system, optionally together with at least one regeneration booster, preferably wherein the effector molecules are introduced by particle bombardment. To this end, new artificial and precisely controllable booster genes and proteins are provided. Further, the modified plant cells are regenerated in a direct or an indirect way. Finally, methods, tools, constructs and strategies are provided to effectively modify at least one genomic target site in a plant cell, to obtain the modified plant cell and to regenerate a plant tissue, organ, plant or seed from the modified plant cell.

The invention discloses a rice laodelphax striatellus resistance gene OsSAMS1 and application thereof. The gene provided by the invention is a DNA (Deoxyribonucleic Acid) molecule as shown in SEQ ID NO.2 and SEQ ID NO.3. The invention also provides a method for preparing the Corresponding proteins coded by the gene provided by the invention are proteins as shown in (a) and (b): (a) an amino acid sequence as shown in SEQ ID NO.1; and (b) a protein which is formed by substitution and / or deletion and / or addition of one or more amino acid residues of the amino acid sequence shown in SEQ ID NO.1, is related to rice laodelphax striatellus resistance and is derived from SEQ ID NO.1. The rice insect-resistant related protein provided by the invention affects the insect resistance of plants. Insect-sensitive plants can resist insects by improving expression of the protein coding gene, and laodelphax striatellus-resistant transgenic plants can be cultivated by silencing the gene. The gene and the encoding protein thereof can be applied to plant genetic improvement.

The invention discloses a method for distinguishing clematis palustris from other clematis plants and application of the method. The method for distinguishing the clematis from the other clematis plants comprises the following steps: detecting whether the genotype of 201st deoxyribonucleotide in a sequence 1 of a plant to be detected is an AA genotype or a CC genotype by adopting a primer pair consisting of a single-stranded DNA (Deoxyribonucleic Acid) shown in a sequence 2 and a single-stranded DNA shown in a sequence 3; the to-be-detected plant is clematis hispida; if the genotype of the plant to be detected is AA genotype, the plant to be detected is other clematis plants. Experiments prove that the method provided by the invention can be used for effectively distinguishing the clematis palustris from other 14 clematis plants, and an important tool is provided for quickly and accurately identifying the clematis palustris in the seedling stage.

The invention belongs to the field of gene engineering, and relates to a chalkiness-related protein OsGAPDH of rice endosperm as well as a coding gene and application of the chalkiness-related protein OsGAPDH. The protein provided by the invention is a protein as shown in (a) or (b): (a) a protein consisting of an amino acid sequence as shown in SEQ ID NO: 3 in a sequence table; and (b) a protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence of SEQ ID NO: 3, is related to plant starch synthesis and is derived from SEQ ID NO: 3. The protein provided by the invention affects normal development of rice endosperm. The coding gene of the protein is introduced into Ningjing No.7 wild type, so that a transgenic plant of which the chalkiness of endosperm is obviously reduced can be cultivated. The protein and the coding gene thereof can be applied to plant genetic improvement.

The invention relates to the technical field of plant breeding, in particular to a mutant of rice ALS protein and application of the mutant. The mutant is obtained by mutating an amino acid sequence of a rice wild-type ALS protein as follows: serine at the 627th site is mutated into threonine, glycine at the 628th site is mutated into histidine, and amino acid at the 629th site is deleted. The application comprises the following steps: (1) enabling plants to have ALS inhibitor herbicide resistance; (2) the resistance of plants to ALS inhibitor herbicides is improved; (3) the phytotoxicity of ALS inhibitor herbicides to plants is reduced; (4) weed control; (5) plant genetic breeding or seed production; and (6) serving as a plant transgenic selection marker. The rice ALS protein mutant is obtained through resistance stress screening, the mutant is reported for the first time, rice can have high resistance to more than two ALS inhibitor herbicides, and the rice ALS protein mutant has high market value and social benefits.

The invention discloses a purification method of a GSL5 protein with disease-resistant and disease-susceptible dual functions, belongs to a protein purification technology, and provides a purification method of a plant gene-GSL5 expression protein with disease-resistant and disease-susceptible dual functions, which comprises the step of fusing two different affinity tags at the C terminal of GSL5, the high-purity target protein GSL5 is obtained through a two-step mild and efficient affinity purification mode, a complex multi-step purification process is avoided, the purification efficiency is improved, and the method can be used for three-dimensional structure research of GSL5 and drug molecule design research based on the structure of GSL5.

The present invention relates to a method for preparing a MAGEA8-Fc transgenic plant. Through a platform using a tobacco plant, a recombinant protein MAGEA8-Fc, in which a human IgG Fc fragment is fused to a MAGEA8 protein, can be stably expressed. The method of the present invention enables high-level expression efficiency and stable protein production by introducing a target gene into a plant genome using an Agrobacterium binary vector. By directly isolating and purifying the recombinant protein from the transgenic tobacco plant, large-scale production can be easily achieved. Compared to conventional microbial or animal cell-based expression systems, the method provides economic efficiency and scalability, and thus can be usefully applied as a platform for producing fusion proteins.

The present application relates to the field of plant genetic breeding, and particularly relates to a kind of evaluation method of asparagus yield. Including the following steps: S1, obtaining young plant period asparagus;S2, measures i variety of young plant period asparagus following parameters: photosynthetic rate A, ribulose-1,5-bisphosphate carboxylase / oxygenase activity B, leaf dry weight per unit area C, acid invertase activity D and root crown ratio E;The measurement sample of the acid invertase activity D is the tender stem of young plant period asparagus at 10-30cm from top;S3, according to the formula, the evaluation value is calculated, and the evaluation value is used to evaluate the yield of asparagus. The evaluation value obtained by using the above measurement index and calculation formula, and the significance level obtained by SPSS software is used to evaluate the yield of asparagus. The yield potential of asparagus can be comprehensively evaluated from the genetic point of view in a short time. Using the evaluation method of the present application, high-yield asparagus varieties can be selected in a short time without tracking for many years.

This invention relates to the field of plant gene extraction technology, specifically to a ligase-based plant gene fragment screening and filtering device. The device includes a workbench, a support frame, a mounting column, a mounting plate, a turntable, and an extraction device. The support frame is fixedly connected to the lower surface of the workbench, the mounting column is fixedly connected to the upper surface of the workbench, and the mounting plate is fixedly connected to the upper surface of the mounting column. The turntable is disposed inside the workbench and is rotatably connected to the surface of the mounting column. The extraction device is mounted on the turntable and includes a support plate and a groove. The support plate is fixedly connected to the lower surface of the workbench. This invention, by setting up the extraction device, allows operators to complete all extraction steps from a single location, reducing operator fatigue and physical exertion. Furthermore, with the addition of four sets of dissolving cups, continuous processing of plant fragments can be achieved, effectively improving the convenience of the equipment.

The present application relates to the field of plant gene map cloning and molecular breeding, and particularly relates to a maize gene DWF4 and related functional sites and uses thereof. Specifically, the present application provides a method for regulating maize plant type based on the DWF4 gene, and a maize plant or part, seed, cell or offspring of the maize plant in which the endogenous DWF4 gene is disrupted, and thus the present application also provides a method for producing a maize plant or hybrid maize plant, and a maize yield-increasing planting method.

The invention relates to a ZmNF-YA13 gene and application thereof in plant disease resistance breeding, and belongs to the technical field of plant gene breeding, the nucleotide sequence of the ZmNF-YA13 gene is shown as SEQ ID NO.1, the amino acid sequence of protein coded by the gene is shown as SEQ ID NO.2, the ZmNF-YA13 gene can be used for regulating and controlling the resistance of plants to corn southern leaf blight, and the ZmNF-YA13 gene can be applied to plant disease resistance breeding. The over-expression of the ZmNF-YA13 gene can enhance the resistance of the plant to the corn southern leaf blight, and the function deletion of the ZmNF-YA13 gene can weaken the resistance of the plant to the corn southern leaf blight. According to the invention, the resistance of the ZmNF-YA13 gene for positively regulating the plant to the corn southern leaf blight is found and verified, and a new gene target and an important theoretical basis are provided for corn breeding for disease resistance.

The invention belongs to the field of gene editing, and particularly relates to efficient sgRNA capable of improving gene editing efficiency and application of the efficient sgRNA, the efficient sgRNA is Vp-sgRNA-01 or Vp-sgRNA-02, the nucleotide sequence of the Vp-sgRNA-01 is shown as SEQ ID NO: 1, and the nucleotide sequence of the Vp-sgRNA-02 is shown as SEQ ID NO: 2; the application refers to the application of editing a plant gene in an efficient CRISPR / VpCas9 gene editing system. The efficient sgRNA provided by the invention is combined with a VpCas9 protein three-site mutant for use, so that the gene editing efficiency reaches up to 85%; the VpCas9 protein mutant provided by the invention can be stably and efficiently expressed in plant cells, so that the expression of a target gene is better regulated and controlled; the efficient CRISPR / VpCas9 gene editing system is high in cutting efficiency and low in miss-target property, the plant gene editing efficiency is greatly improved, and the efficient CRISPR / VpCas9 gene editing system has wide application prospects in the field of plant gene editing.