Method for deleting selection marker gene of transgenic rice

A technology for screening marker genes and transgenic rice, which is applied in the fields of biotechnology and plant genetic engineering, can solve the problems of unfavorable large-scale operation and low efficiency of molecular deletion system, achieve broad development potential and application prospects, efficient directional deletion, and simple operation quick effect

Active Publication Date: 2015-08-19
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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Problems solved by technology

At present, the efficiency of the molecular deletion system applied in the rice system is relatively low

Method used

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  • Method for deleting selection marker gene of transgenic rice
  • Method for deleting selection marker gene of transgenic rice
  • Method for deleting selection marker gene of transgenic rice

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Embodiment Construction

[0045] The present invention will be described below with reference to specific examples. Those skilled in the art can understand that these examples are only used to illustrate the present invention and do not limit the scope of the present invention in any way.

[0046] The experimental methods in the following examples are conventional methods unless otherwise specified. The medicinal raw materials, reagent materials, etc. used in the following examples are all commercially available products unless otherwise specified.

[0047] Vector construction

[0048] In this example, the vector pCAMBIA1300 was used to obtain Nipponbare transgenic plants with selection markers. In addition, the screening marker gene PMI and the reporter gene GUS are taken as examples for illustration. According to the characteristics of the PMI coding frame in the obtained transgenic rice containing the PMI marker gene, a suitable targeting site is selected. The present invention selects the target...

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Abstract

The invention provides a method for deleting a selection marker gene of transgenic rice and belongs to the field of plant gene engineering. The method for efficiently deleting the marker gene of the transgenic rice in a plant level is established by utilizing a genome fixed-point editing technology mediated by a CRISPR/Cas9 system. The whole segment of the marker gene can be effectively deleted by utilizing the method, and only an expression frame of the marker gene is deleted pointedly without change of the expression of other components in the transgenic rice. Thus, the transgenic rice without the selection marker gene can be efficiently bred by utilizing the method, so that the safety doubt of people about the selection marker gene can be completely removed.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and plant genetic engineering. Specifically, the present invention relates to a method capable of deleting a selection marker gene of a transgenic plant. Background technique [0002] In transgenic plants, in addition to the target gene, the exogenous genes introduced into the plant also include selection marker genes and other related genes. The target gene is a gene used to optimize or impart specific traits to plants; the screening marker gene can endow transgenic plants with resistance to certain antibiotics or herbicides and other characteristics, and its use in the transgenic process can greatly improve the efficiency of transgenic plant screening. In addition to the target gene, people's concerns about transgenic plants are mainly focused on the screening marker gene. Although there is no definite evidence that the screening marker gene is indeed a safety hazard, the safety of the s...

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Application Information

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IPC IPC(8): C12N15/82A01H5/00
Inventor 李娟杨剑波邱春红魏鹏程马卉李莉杨亚春李浩秦瑞英
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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