Method for deleting selection marker gene of transgenic rice

A technology for screening marker genes and transgenic rice, which is applied in the fields of biotechnology and plant genetic engineering, can solve the problems of unfavorable large-scale operation and low efficiency of molecular deletion system, achieve broad development potential and application prospects, efficient directional deletion, and simple operation quick effect
CN104846010AActive Publication Date: 2015-08-19RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
1 Cites 51 Cited by

Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
Publication Date
2015-08-19

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides a method for deleting a selection marker gene of transgenic rice and belongs to the field of plant gene engineering. The method for efficiently deleting the marker gene of the transgenic rice in a plant level is established by utilizing a genome fixed-point editing technology mediated by a CRISPR / Cas9 system. The whole segment of the marker gene can be effectively deleted by utilizing the method, and only an expression frame of the marker gene is deleted pointedly without change of the expression of other components in the transgenic rice. Thus, the transgenic rice without the selection marker gene can be efficiently bred by utilizing the method, so that the safety doubt of people about the selection marker gene can be completely removed.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical fields of biotechnology and plant genetic engineering. Specifically, the present invention relates to a method capable of deleting a selection marker gene of a transgenic plant. Background technique

[0002] In transgenic plants, in addition to the target gene, the exogenous genes introduced into the plant also include selection marker genes and other related genes. The target gene is a gene used to optimize or impart specific traits to plants; the screening marker gene can endow transgenic plants with resistance to certain antibiotics or herbicides and other characteristics, and its use in the transgenic process can greatly improve the efficiency of transgenic plant screening. In addition to the target gene, people's concerns about transgenic plants are mainly focused on the screening marker gene. Although there is no definite evidence that the screening marker gene is indeed a safety hazard, the safety of the s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More