A method for deleting the selection marker gene of transgenic rice

A technology for screening marker genes and transgenes, applied in the field of biotechnology and plant genetic engineering, can solve the problems of low efficiency of molecular deletion system, unfavorable large-scale operation, etc., achieve broad development potential and application prospects, efficient directional deletion, strong pertinence Effect

Active Publication Date: 2018-07-06
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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Problems solved by technology

At present, the efficiency of the molecular deletion system applied in the rice system is relatively low, and it needs to be induced in the tissue culture stage, which is not conducive to large-scale operation

Method used

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  • A method for deleting the selection marker gene of transgenic rice
  • A method for deleting the selection marker gene of transgenic rice
  • A method for deleting the selection marker gene of transgenic rice

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Embodiment Construction

[0045] The present invention will be described below with reference to specific examples. Those skilled in the art can understand that these examples are only used to illustrate the present invention and do not limit the scope of the present invention in any way.

[0046] The experimental methods in the following examples are conventional methods unless otherwise specified. The medicinal raw materials, reagent materials, etc. used in the following examples are all commercially available products unless otherwise specified.

[0047] Vector construction

[0048] In this example, the vector pCAMBIA1300 was used to obtain Nipponbare transgenic plants with selection markers. In addition, the screening marker gene PMI and the reporter gene GUS are taken as examples for illustration. According to the characteristics of the PMI coding frame in the obtained transgenic rice containing the PMI marker gene, a suitable targeting site is selected. The present invention selects the target...

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Abstract

The invention provides a method for deleting a transgenic rice marker gene, which belongs to the field of plant genetic engineering. The present invention utilizes the genome fixed-point editing technology mediated by the CRISPR / Cas9 system to establish a method for efficiently deleting marker genes of transgenic rice at the plant level. The method of the invention can effectively delete the entire marker gene, and can delete only the expression frame of the marker gene in a targeted manner without changing the expression of other components in the transgenic rice. Therefore, the method of the present invention can be used to efficiently breed transgenic rice without a selection marker gene, thereby completely eliminating people's doubts about the safety of the selection marker gene.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and plant genetic engineering. Specifically, the present invention relates to a method capable of deleting a selection marker gene of a transgenic plant. Background technique [0002] In transgenic plants, in addition to the target gene, the exogenous genes introduced into the plant also include selection marker genes and other related genes. The target gene is a gene used to optimize or impart specific traits to plants; the screening marker gene can endow transgenic plants with resistance to certain antibiotics or herbicides and other characteristics, and its use in the transgenic process can greatly improve the efficiency of transgenic plant screening. In addition to the target gene, people's concerns about transgenic plants are mainly focused on the screening marker gene. Although there is no definite evidence that the screening marker gene is indeed a safety hazard, the safety of the s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82A01H5/00A01H6/46
Inventor 李娟杨剑波邱春红魏鹏程马卉李莉杨亚春李浩秦瑞英
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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