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58 results about "Genetics dna" patented technology

The molecular basis for genes is deoxyribonucleic acid (DNA). DNA is composed of a chain of nucleotides, of which there are four types: adenine (A), cytosine (C), guanine (G), and thymine (T). Genetic information exists in the sequence of these nucleotides, and genes exist as stretches of sequence along the DNA chain.

Method for massively and efficiently developing molecular markers on basis of Indel and SSR (simple sequence repeat) site techniques

The invention discloses a method for massively and efficiently developing molecular markers on the basis of Indel and SSR (simple sequence repeat) site techniques. The method comprises the following steps: (1) selecting at least 3 samples to be developed, and respectively extracting DNAs (deoxyribonucleic acids) of the samples to be developed; (2) carrying out enzyme digestion on the DNA samples of the samples to be developed, establishing a sequencing library, and carrying out sequencing; (3) mixing the genomes of all the samples to be developed, and assembling to obtain Contigs; (4) comparing the Contigs with the sequences of the sample individuals to be developed, and acquiring SSR sites with Indel inside according to the Indel and SSR site information as a candidate polymorphism SSR site; (5) designing primers according to the obtained candidate polymorphism SSR site, carrying out PCR (polymerase chain reaction) amplification and sequencing, and selecting a ribbon-shaped stable clear strip as molecular marker primers to be verified; and (6) carrying out PCR amplification on different samples to be developed by using the obtained molecular marker primers, and selecting the molecular markers with diversity, thereby obtaining the molecular markers. The method enhances the molecular marker development efficiency; and the developed SSR molecular markers can be efficiently applied to research in the aspects of genetics, multiplication release evaluation and the like.
Owner:SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI

Scophthalmus maximus T170G single nucleotide polymorphic marking detection method

The invention relates to a scophthalmus maximus T170G single nucleotide polymorphic marking detection method. The method comprises the following steps: extracting scophthalmus maximus genome DNA and diluting for later use; analyzing the sequences of EST, screening the sequence containing a candidate SNP locus, designing a specific primer at the two ends and designing a non-marking probe with the closed 3' end in front of and behind the locus (comprising the locus); performing asymmetric PCR amplification on the scophthalmus maximus group genome DNA by using the primer; hybridizing the amplification product with the non-marking probe with the closed 3' end; and placing the hybrid product on a LightScanner, and detecting and analyzing the melting curve to obtain the genetic polymorphism mapof the scophthalmus maximus. The method is applicable to detection technologies of scophthalmus maximus genetic marking, genealogy authentication, genetic linkage map construction and the like. According to the method which is convenient, quick and accurate, the scophthalmus maximus T170GSNP marked genetic variation map can be obtained quickly; and the genotype of each individual of the scophthalmus maximus can be detected intuitively.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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