55 results about "Scophthalmus" patented technology

The invention discloses a method for constructing a kidney cell line of scophthalmus maximus, which comprises the following steps of: preparing a cell culture fluid first, taking a kidney tissue of the scophthalmus maximus as a material, using 0.5 percent trypsinase to digest the kidney tissue for 30 minutes at room temperature after the rinsing and shearing, adopting an 200-mesh nylon gauze to filter and collect cells, split charging the cells with the quantity of 5-10*10 counts/bottle into culture bottles with the growth area of 25 cm, and placing the culture bottles at a temperature of 24 DEG C for culturing; replacing the cell culture fluid with half quantity each four days, and using 0.25 percent trypsinase digest the cells for passage when primary cells grow to be monolayered; and performing passage once each 8 to 10 days, wherein the content of serum in the cell culture fluid is reduced to 10 percent from 20 percent when the 8th generation is reached, and the cell line is successfully established at the moment. The kidney cell line of the scophthalmus maximus obtained by utilizing the method is fibroid and can be continuously transferred for more than 40 generations; the cell line can be directly applied to pathogeny characteristics research, vaccine development and functional gene research; and the construction method is suitable to construct kidney cell lines of other fishes.

The invention relates to animal compound protein. The animal compound protein is characterized containing meat and bone meal, blood powder, chicken powder and fermented silkworm chrysalis meal. The animal compound protein comprises the following components in parts by weight: 3.42-3.83 parts of the meat and bone meal, 4.18-4.54 parts of the blood powder, 11.62-13.35 parts of the chicken powder and 5.11-6.19 parts of the fermented silkworm chrysalis meal. In the preparation process, all the components are ground into fine powder and are sieved by a sieve of 80 meshes; the components are respectively weighed and are gradually and uniformly mixed. The efficient animal compound protein provided by the invention can effectively replace fish meal protein in scophthalmus maximus feed; the survival, the opisthosoma weight, the specific growth rate, the hepatosomatic index, the organ index and the condition factor of scophthalmus maximus are not influenced; all indexes in blood serum are not influenced; the body composition of regular fish bodies of the scophthalmus maximus is also not influenced. The aims of lowering the price of the feed and saving the cultivation cost are realized and theoretical foundation is provided for the sustainable development of aquaculture.

The invention discloses an energy-saving and emission-reducing scophthalmus maximus seedling culture method. The method comprises the following steps of: (1) selection of parent fish; (2) parent fish enrichment culture; (3) egg taking, fertilization and hatching; (4) distribution in a pond; (5) bait feeding; and (6) harvesting. By adopting the technical of combining prevention and adjustment of bdellovibrio nectophore and leech plasmids and environmental control, mixed bacteria solution of the bdellovibrio nectophore and the leech plasmids is added into a seedling culture pond, and the concentration of the mixed bacteria solution in the water body is 10 to 10<7> pfu/mL; meanwhile, the feed is soaked into the 10<7> pfu/mL mixed bacteria solution of the bdellovibrio nectophore and the leechplasmids, the average seedling culture survival rate reaches over 50 percent, the whitening rate is reduced to 3.4 percent, and the aberration rate is reduced to 2.3 percent; and the method can effectively control the bacteria number in the water body, improves the water quality, does not need to change water in 40 days, saves the cost, greatly improves the economic benefit, and is a green low-carbon novel pollution-free seedling culture method.

The objective of the invention is to provide a pathogenic strain separated from tissue of Scophthalmus maximus with an edge-rot disease. The pathogenic strain is Vibrio anguillarum which is preserved in China General Microbiological Culture Collection Center, No. 3, Yard 1, West Beichen Road, Chaoyang District, Beijing on June 17th, 2013, with an accession number of CGMCC No. 7733. The screened Vibrio anguillarum is used for preparation of an inactivated vaccine. A preparation method for the inactivated vaccine for the Scophthalmus maximus edge-rot disease has the advantages of simple process, stable output, low cost and capacity of obtaining a great number of the inactivated vaccine with high immune effects. The inactivated vaccine prepared by using the preparation method has an immune protective rate of 68.5%, is a purely biological preparation, has the characteristics of safety and environment friendliness, does not generate drug residue, poses no pollution to the environment, fundamentally suppresses occurrence and prevalence of the Scophthalmus maximus edge-rot disease and provides technical support for large scale preparation of the inactivated vaccine for the Scophthalmus maximus edge-rot disease.

The invention belongs to the technical field of aquatic product processing and storage, and relates to a rapid evaluation method of the freshness of Scophthalmus maximus. The equations of remaining shelf lives at a constant temperature and a fluctuation temperature which are in a range of 0-25DEG C comprise an equation (4) and an equation (5). In the equation (4) and the equation (5), SLT is the shelf life when the temperature is T, and RSLT is the remaining shelf life when the temperature is T; and T is the temperature, and RRSTn is the relative corrosive rate predication value when the temperature is Tn. The method has the advantages of rapidness, simplicity and small deviation.

The invention relates to a positioning marking method of PGCs, and concretely relates to a positioning marking method for primordial germ cells (PGCs) of scophthalmus maximus at embryonic development stage. The method comprises: acquiring embryo samples of scophthalmus maximus at all stages, fixing, using methanamide with the concentration of 50% to store the samples at -20 DEG C to 40 DEG C for usage; removing oolemma of all above embryo samples of scophthalmus maximus at all stages, using 1*PBST-methanol eluants with different concentrations to perform gradient dewatering processing, and storing at -20 DEG C to -40 DEG C; employing gradient methanol o process the embryo samples at all stages and subjected to above dewatering processing for rewatering, then using a 1*PBST buffer to wash, and after eluting, pre-hybridizing at 60-65 DEG C for 1-2 h; after prehybridization, hybridizing in a hybridization liquid containing a scophthalmus maximus RNA probe at 60-65 DEG C for a night; and after hybridization, washing, performing antibody incubation and color development, and further performing positioning marking. The invention provides the relatively convenient and practical sample storage method, the problem that oolemma and yolk are difficult to remove because methanol dewatering and storage are performed during conventional embryo integral in-situ hybridization is solved, and a protease K digestion step is saved, and the operation steps are simplified.

A Scophthalmus maximus rahbdovirus (SMRV0207, CCTCC No.202006) is prepared through sampling the liver, kidney, heart and spleen tissues from the scophthalmus maximus in ill, shearing, adding PBS, homogenizing, adding double antibody, freezing, thawing, centrifugal extracting, filtering, inoculating it to CLC 0207 cell, and constant-temp. continuous culture. Its advantages are high reproduction speed, and high reproduction valency up to 10 to the power 8 TCID 50/ml. It can be used to prepare vaccine.

The invention aims to provide a high-efficiency plant compound protein. The high-efficiency plant compound protein is prepared from the following components in parts: 4.85 to 5.41 parts of peanut meal, 8.92 to 9.81 parts of corn protein powder, 8.18 to 8.99 parts of soya bean meal, 2.37 to 2.72 parts of wheat gluten and 10.93 to 12.04 parts of cottonseed meal. The plant compound protein is used for replacing fish meal to prepare a scophthalmus maximus feed. The plant compound protein provided by the invention can effectively replace 70 percent of fish meal protein in an all-fish meal feed of the scophthalmus maximus feed; the final body weight, the specific growth rate, the survival rate, the fatness, the ratio of liver weight to body weight and the ratio of viscera weight to body weight of scophthalmus maximus are not influenced; the body compositions of a conventional body of the scophthalmus maximus are not influenced; the digestion rate of dry matters and proteins of the feed is not influenced; various indexes in serum are not influenced. The purposes of reducing feed cost and reducing cultivation cost are achieved, and theoretical basis is provided for sustainable development of aquaculture.

The invention relates to a Chinese herb composition capable of effectively treating the dark-thin syndrome of Scophthalmus maximus, wherein the Chinese herb composition comprises, by weight, 10-20 parts of rhus chinensis mill, 25-35 parts of pulsatilla chinensis (bunge) regel, 45-55 parts of cortex phellodendri, 15-25 parts of andrographis paniculata, 20-35 parts of angelica sinensis, 15-25 partsof codonopsis pilosula, 12-24 parts of honeysuckle, 10-20 parts of eucommia ulmoides, 8-15 parts of cortex fraxini, 6-15 parts of frankincense, and 2-5 parts of myrrh. According to the present invention, the components are subjected to scientific compatibility according to the traditional Chinese medicine principle of assistant and guide, the effective components of most of the herbs are subjectedto quantitative and semi-quantitative detection by using the modern scientific and technological means, and the prepared finished product can provide good treatment effect for the dark-thin syndromeof Scophthalmus maximus; various Chinese herbs acts synergistically, such that the inhibition effect on pathogen is enhanced, and the toxicity of the single herb is reduced; and the Chinese herb composition can significantly prevent and treat the dark-thin syndrome of Scophthalmus maximus, is the traditional Chinese medicine with characteristics of good treatment effect, no pollution, no residue and easy use, and is especially suitable for the large-scale industrial breeding of Scophthalmus maximus.

Parasites on the body surface of fishes are removed without causing side effects by orally administering bithionol to fishes in an amount of 50 to 250 mg/kg of body weight per day. Bithionol is orally administered for 1 to 3 days every day or every other day. This method is suitable for fishes of the order Perciformes, Pleuronectiformes and Tetraodontiformes such as amberjack, yellow tail, Seriola lalandi, Seriola rivoliana Valenciennes, cobia, common horse mackerel, Japanese horse mackerel, Japanese seaperch, Pagrus major, Zebrasoma flavescens, Epinephelus akaara, Epinephelus bruneus, Japanese flounder, Verasper moseri, Takifugu rubripes,Oplegnathus fasciatus, Oplegnathus punctatus, Scomber japonicus, threadsail filefish, Scophthalmus aquosus and tilapia, which are parasitized with Neobenedenia girellae. When removing parasites on the body surface of fishes, feed for fish culture supplemented with a necessary amount of bithionol can be used.

The invention discloses an energy-saving method for culturing scophthalmus maximus. The method comprises the following steps of: selecting a culturing water source; treating water in a pool; treating a feed; controlling a water changing period; and controlling a culturing environment by a bio-ecology method according to the bio-ecology principle of a culturing water body, the requirement of marine organisms on growth environments, mutual relation between organisms and a nuisanceless culturing standard. Key technology is that: bdellovibrio bacteriovorus plasmid bacterial liquid is added into awater body; the feed is soaked in the bdellovibrio bacteriovorus plasmid bacterial liquid; and the water in the pool can be changed once every 40 days in the culturing period. The method is a biological culturing method, which is environmentally-friendly, safe and pollutant-free, has remarkable effect and extremely good development prospect, can improve water quality, promotes ingestion of the scophthalmus maximus, enhances immunity, promotes growth and increases survival rate. The method plays an important role in enhancing economic benefit and eating safety and guaranteeing human health.

The invention relate to powder feed for aquaculture animals, in particular to special powder feed for juvenile scophthalmus maximus, and further relates to a preparation method of the special powder feed for the juvenile scophthalmus maximus. The special powder feed for the juvenile scophthalmus maximus comprises components in percentage by weight as follows: 45%-55% of meat and bone meal, 10%-15% of bean pulp, 8%-10% of kelp powder, 8%-12% of wheat bran, 8%-12% of rapeseed meal, 1%-2% of shell powder, 1%-3% of dried hawthorn fruit powder, 1%-3% of honey and 1%-2% of a multivitamin complex. The special powder feed for the juvenile scophthalmus maximus and the preparation method of the special powder feed have the advantages of low production cost and high protein efficiency, and the special growth rate of the juvenile scophthalmus maximus fed with the powder feed is high.

The invention discloses a scophthalmus maximus culture method. The scophthalmus maximus culture method comprises the steps of 1 pond selection, 2 seedling selection, 3 fingerling stocking and 4 feed throwing. The scophthalmus maximus culture method has the advantages of reducing the culture cost, improving the productive profits of farmers, shortening a culture period and the like.

The invention discloses application of sapindus mukorossi Gaertn. in preparing a drug for resisting paralembus digitiformis, and belongs to the technical field of medicine. The sapindus mukorossi Gaertn. can be prepared into powder with any acceptable auxiliary material, then the powder is added into feed, and then scophthalmus maximus suffering from paralembus digitiformis diseases is fed with the feed; or the sapindus mukorossi Gaertn. is subjected to ethanol extraction, the auxiliary material is added to prepare liquid, and then the scophthalmus maximus suffering from paralembus digitiformis diseases is subjected to a medicated bath. According to the application of the sapindus mukorossi Gaertn. in preparing the drug for resisting the paralembus digitiformis, it is found for the first time that the sapindus mukorossi Gaertn. can significantly lower the death rate of the paralembus digitiformis diseases of the scophthalmus maximus, and the infection rate of the paralembus digitiformis is lowered.

The invention discloses an application of Bdellovibrio nectophore in preparing a bactericide for controlling the red mouth disease of a scophthalmus maximus. Bdellovibrio is Bdellovibrio sp. BDM01. In the application method of the bactericide, the bactericide for controlling the scophthalmus maximus red mouth disease is splashed into a water body to cause the final concentration of the Bdellovibrio nectophore in the water body to be 10-107pfu/mL, or feeds are soaked by the bactericide for controlling the scophthalmus maximus red mouth disease for 15-45min to cause the concentration of the Bdellovibrio nectophore in the bactericide to be 10-107pfu/mL. The application of the Bdellovibrio nectophore in preparing the bactericide for controlling the scophthalmus maximus red mouth disease can effectively control and treat the red mouth disease of the scophthalmus maximus and has high safety, environmental protection and good application prospect and does not generate pathogenic bacteria resistance in the application of aquatic animal culture.

The invention discloses a cytology division method of a scophthalmus maximus spermiogenesis phase. The method comprises the following steps: fixing, pre-dyeing, dehydrating and embedding, dyeing by uranyl acetate, dyeing by lead citrate, and slicing preparing and analysis. A scophthalmus maximus spermiogenesis process is divided into 8 phases: comprising sperm cell I-VII stage and mature sperm. The method has the beneficial effects that the cytology division method overcomes the problem that during common histological slice observation, sperm cells at different metamorphosis periods cannot bedistinguished due to limited magnification times, pre-dyeing is helpful for increasing dyeing uniformity, tissue structure definition, and tissue dyeing contrast grade, dyeing effect can be optimized,and image definition of the tissue structure of the sperm cells can be obviously enhanced; the cytology division method can fill the research blank of phase division for the flounder paralichthys spermiogenesis, and can be used for cytology division of the spermiogenesis phase of the other seawater fish.

The present invention discloses a compound feed for scophthalmus maximus and a preparation method thereof. The compound feed comprises iced fresh fish and concentrated materials. The mass ratio of the iced fresh fish and the concentrated materials is (3-7):(3-7). The provided compound feed for the scophthalmus maximus has the advantages of being comprehensive in nutrition, small in feeding amount and good in attractant of the iced fresh fish.

The invention belongs to the field of feed additives of aquatic animals and discloses a composite Chinese herbal medicine growth promoter for young scophthalmus maximus and preparation and use methodsthereof. The growth promoter comprises the following ingredients in parts by mass: 20 to 25 parts of radix glycyrrhizae, 25-30 parts of rhizoma dioscoreae, 25-30 parts of radix angelicae sinensis and15-20 parts of medicated leaven. The composite Chinese herbal medicine growth promoter can be used for remarkably promoting the growth of the young scophthalmus maximus, the coefficient of feed (feedconversion ratio) is reduced, and the turnover of funds is accelerated; the composite Chinese herbal medicine growth promoter is free of toxic or side effects, is pollution-free to fish bodies and environments, is cheap in raw materials, convenient in processing and use and convenient to popularize and plays an significant role in developing the culture of scophthalmus maximus in the future.

The invention discloses a turbot-sourced aeromonas salmonicida attenuated strain and application thereof.The attenuated strain is a high-temperature derivative strain of aeromonas salmonicida for killing Japanese salmonella subspecies, namely aeromonas salmonicida AS-1, is classified and named as aeromonas salmonicida AS-1, is preserved in China Center for Type Culture Collection, the preservation address is Wuhan, China, and the preservation number is CGMCC NO.12487. The invention further discloses a method for preparing the turbot-sourced aeromonas salmonicida attenuated strain. The preservation date is February 18, 2022, and the preservation number is CCTCC (China Center For Type Culture Collection) NO: M 2022131. Through artificial infection, the aeromonas salmonicida AS-1 has low toxicity to scophthalmus maximus, a live vaccine prepared from the aeromonas salmonicida AS-1 is used for immune protection of the scophthalmus maximus, bacterial diseases caused by the fact that the aeromonas salmonicida kills subspecies Japanese salmonicida can be effectively prevented, and the aeromonas salmonicida AS-1 plays a positive role in reducing abuse of antibiotic drugs and guaranteeing quality safety of aquatic products.