12 results about "Scophthalmus" patented technology

The invention discloses a scophthalmus maximus anti-edwardsiella tarda SNP molecular marker and application thereof, and belongs to the technical field of molecular breeding and biology. The nucleotide sequence of the SNP molecular marker is shown as SEQ ID No.1, and the polymorphic site of the SNP molecular marker is A / C. The invention further provides application of the SNP molecular marker in screening of turbots with the Edwardsiella tarda resistant character, and the CC genotype turbots are individuals with the Edwardsiella tarda resistant character. By utilizing the SNP molecular marker provided by the invention, DNA level selection can be carried out in the early stage of fish fries, interference of environmental factors is avoided, the accuracy and efficiency of breeding are remarkably improved, and the breeding period is shortened. The molecular marker is remarkably verified in an independent verification group through linear regression analysis, genotype-phenotype association is stable and reliable, false positive is eliminated, and the molecular marker has a good market application prospect.

The invention relates to an SNP (Single Nucleotide Polymorphism) molecular marker associated with the fatness of turbots fed with fermented soybean meal and application of the SNP molecular marker, and belongs to the technical field of molecular markers. The SNP molecular marker obviously associated with the fatness character is obtained by analyzing the fatness character of the turbots through genome-wide association analysis and comprises at least one of six SNP loci; the scophthalmus maximus molecular marker can be applied to scophthalmus maximus molecular assisted breeding, screening is conducted on the genome level through the breeding method, the breeding period can be greatly shortened, dependence on phenotypic characters is reduced, the selective breeding efficiency is improved, and the scophthalmus maximus molecular marker has good application prospects.

The invention relates to an application of a DIO1 gene in a liver in controlling the body color of a turbot, and belongs to the field of molecular biology, and the application method is as follows: the turbot with golden body color is obtained by overexpressing the DIO1 gene in the liver of the turbot; the amino acid sequence of the protein coded by the DIO1 gene is as shown in SEQ ID NO. 1. The invention discovers that the gene expression level change in the liver can cause the change of the blood hormone level by influencing the thyroid hormone pathway in the turbot for the first time, thereby influencing the color formation of the skin; therefore, the formation of the target body color of the scophthalmus maximus can be controlled by controlling the gene expression level, so that the market value of the scophthalmus maximus is improved.

The present application belongs to the field of artificial breeding of Scophthalmus maximus, in particular to a separating device for Scophthalmus maximus production, which comprises a cylinder, a support column with an installation slot at the top end fixed in the middle of the inside of the cylinder, a rotating drive mechanism installed in the inside of the installation slot, an output shaft of the rotating drive mechanism connected with a rotating disc rotatingly connected at the top end of the support column, four electrically energized fast separating Scophthalmus maximus racks evenly arranged at the edge of the rotating disc, and four Scophthalmus maximus racks corresponding to the feeding position, the cleaning position, the egg collecting position and the discharging position respectively. The rotating drive mechanism drives the rotating disc and the Scophthalmus maximus racks thereon to adjust the position, so as to switch the position of Scophthalmus maximus in the Scophthalmus maximus racks at the feeding position, the cleaning position, the egg collecting position and the discharging position, and the device integrates the feeding, cleaning, egg collecting and discharging of Scophthalmus maximus, has high egg collecting efficiency of Scophthalmus maximus, low labor intensity and uniform mechanical egg collecting force with small damage to the eggs.

The invention relates to a compound feed for breeding low-fat scophthalmus maximus, and the compound feed comprises 8.1-32.2% of chenopodium quinoa willd germ powder and 36-48% of fish meal on the basis that the dry weight of the compound feed is 100% by weight. On the basis that the dry weight of the compound feed is 100 wt%, the compound feed comprises 8.1-32.2% of quinoa germ powder, 36-48% of fish meal, 12.3-24% of wheat flour, 4.4-9% of vital gluten, 4-4.2% of fish oil, 1-2% of soybean lecithin, 1-2% of vitamins, 1-2% of minerals, 0.4-0.6% of choline chloride, 0.8-1.4% of monocalcium phosphate and 1-3% of squid powder. The chenopodium quinoa is used as a feed protein source to replace fish meal, the dosage of the fish meal can be effectively reduced, the lipid content in fish meat is reduced under the condition of ensuring the growth of fish bodies, and the chenopodium quinoa feed has important significance in solving the problem of aquatic feed protein sources and improving the quality of the fish meat.

The invention provides a scophthalmus maximus seedling stage feeding method capable of improving disease resistance, which is mainly characterized in that the feeding method comprises the step of feeding 10-20 days of beta-glucan to 1-month-old and 3-month-old young scophthalmus maximus respectively. According to the scophthalmus maximus seedling stage feeding method capable of improving the disease resistance, through short-term feed adding and mixing feeding and induction of scophthalmus maximus juvenile fish training immune activation, the use cost is effectively reduced, meanwhile, the disease resistance of scophthalmus maximus from the seedling stage to the culture stage is enhanced, and the scophthalmus maximus seedling stage feeding method is expected to be matched with vaccination to improve the disease resistance of the scophthalmus maximus in the whole culture period; the method has a good application prospect in the fields of animal feed addition and disease immune prevention and control.

The application provides application of a camel hoofer in preparation of a medicine for preventing or treating a parasitic infection or a complication caused by the parasitic infection or a fish feed, and belongs to the field of veterinary drugs. The camel hoofer has an excellent effect of killing a naid, and in-vivo experiments show that the camel hoofer has an effect of preventing and treating a naid disease of Scophthalmus maximus. The camel hoofer has little toxicity to Scophthalmus maximus, has no disadvantages of drug residue detection, reduces use of chemical drugs in Scophthalmus maximus culture, and reduces pollution of a marine environment caused by use of chemical agents.

This invention discloses a SNP molecular marker for resistance to Edwardsiella tarda in turbot and its application, belonging to the fields of molecular breeding and biotechnology. The nucleotide sequence of the SNP molecular marker is shown in SEQ ID No. 1, and the polymorphic site of the SNP molecular marker is A / C. This invention also provides the application of the SNP molecular marker in screening turbot with resistance to Edwardsiella tarda; turbot with the CC genotype are individuals with resistance to Edwardsiella tarda. Using the SNP molecular marker provided by this invention, DNA-level selection can be performed in the early stages of fish fry development, unaffected by environmental factors, significantly improving the accuracy and efficiency of breeding and shortening the breeding cycle. The molecular marker was significantly validated in an independent validation population through linear regression analysis, demonstrating stable and reliable genotype-phenotype association, excluding false positives, and showing good market application prospects.

The present application relates to the SNP molecular marker of Scophthalmus maximus fermented soybean meal feeding under the correlation of fatness and its application, belongs to the molecular marker technical field, the present application utilizes the whole genome correlation analysis to carry out the analysis to the fatness character of Scophthalmus maximus, obtains the SNP molecular marker of the significant correlation of fatness character, including at least one of 6 SNP sites, can be applied to the molecular assisted breeding of Scophthalmus maximus, through the screening of the breeding method disclosed in the present application in the genomic level, can greatly shorten the breeding cycle, reduce the dependence on the phenotype character, improve the selection breeding efficiency, and have good application prospect.

The application provides a high-throughput tracking measurement method for growth phenotypes of individual Scophthalmus maximus, a medium, a device and an application thereof, and belongs to the technical field of biological identification. The method uses an image acquisition system to obtain complete images of the ventral surface and the dorsal surface of the Scophthalmus maximus, and performs pretreatment, and uses a machine learning algorithm to identify fish body ventral images that are easy to distinguish; then, a fish fin image of the Scophthalmus maximus is extracted from the dorsal image of an un-identified individual by using an image segmentation model, a deep learning algorithm is used to extract features, and a high-complexity fin image is identified; thereby, all Scophthalmus maximus individuals are identified; then, a data generation model is trained for the dorsal image of the Scophthalmus maximus individual, growth phenotype key point detection is completed, and growth traits of the Scophthalmus maximus are calculated according to key point data. The application also provides a medium, a device and an application for running the method. The method can track growth phenotype information of the whole growth cycle of the Scophthalmus maximus individual, automatically complete growth index detection, and has high precision.

The invention provides a scophthalmus maximus feed and a preparation method thereof. The turbot feed comprises the following components: 42 to 60 weight percent of fish meal, 8 to 30 weight percent of quinoa germ powder, 4 to 10 weight percent of vital gluten, 3 to 8 weight percent of fish oil, 8 to 25 weight percent of wheat flour, 0.8 to 5 weight percent of soybean lecithin, 0.5 to 3 weight percent of vitamin mixture, 0.5 to 3 weight percent of mineral mixture, 0.05 to 2 weight percent of choline chloride, 1 to 3 weight percent of additive, 0.01 to 0.1 weight percent of antioxidant and 0.01 to 2 weight percent of restrictive amino acid. The scophthalmus maximus feed obtained by partially replacing fish meal with the quinoa germ powder can meet the requirements of scophthalmus maximus for all protein, fat, amino acid and other key nutrients for growth and development of the scophthalmus maximus, so that the scophthalmus maximus feed can further solve the problems of slow growth and intestinal inflammation of fishes in the existing feed.

The application provides a method for tracking and identifying individual turbot, a medium, a device and its breeding application, and belongs to the technical field of biological identification. The method is characterized in that: pictures of the ventral surface and the dorsal surface of turbot are collected, and the collected turbot pictures are pretreated; the PCA algorithm is used for dimension reduction on the abdominal image of the turbot sample; by setting the Euclidean distance constraint condition, the abdominal texture image of the turbot that does not meet the condition is removed, so that individual identification is realized; for the individual image that does not meet the condition and is removed, the fin texture is used as the basis, and a deep learning model algorithm is used to segment the fin on the image; based on the obtained image dataset of the fin, a deep learning network is designed, and the identification of all samples is completed. The application also provides a computer readable storage medium and a device for running the identification method, and an application of the method in breeding. The method has high overall recognition rate; one growth cycle of turbot is monitored, and the monitoring task from the seedling stage to the adult fish is completed.