121 results about "Genetic dna" patented technology

A thermoelectric cooling and heating device including a substrate, a plurality of thermoelectric elements arranged on one side of the substrate and configured to perform at least one of selective heating and cooling such that each thermoelectric element includes a thermoelectric material, a Peltier contact contacting the thermoelectric material and forming under electrical current flow at least one of a heated junction and a cooled junction, and electrodes configured to provide current through the thermoelectric material and the Peltier contact. As such, the thermoelectric cooling and heating device selectively biases the thermoelectric elements to provide on one side of the thermolectric device a grid of localized heated or cooled junctions.

Multiple modality contrast can be used to produce images that can be combined and rendered to produce images similar to those produced with wavelength absorbing stains viewed under transmitted white light illumination. Images obtained with other complementary contrast modalities can be presented using engineered color schemes based on classical contrast methods used to reveal the same anatomical structures and histochemistry, thereby providing relevance to medical training and experience. Dark-field contrast images derived from refractive index and fluorescent DAPI counterstain images are combined to produce images similar to those obtained with conventional H&E staining for pathology interpretation. Such multi-modal image data can be streamed for live navigation of histological samples, and can be combined with molecular localizations of genetic DNA probes (FISH), sites of mRNA expression (mRNA-ISH), and immunohistochemical (IHC) probes localized on the same tissue sections, used to evaluate and map tissue sections prepared for imaging mass spectrometry.

A fully automated protein and/or DNA gene fragment analyzing machine and method are disclosed in which, in a simple machine structure, a plurality of electrophoresis calls each containing such fragments, are robotically, under computer programming, inserted into an electrophoresis housing and subjected to voltage for producing electrophoretic migration in one dimension (horizontally), and then preferably after robotic 90 DEG rotating of the cells, are voltage migrated in an orthogonal direction to separate the fragments vertically, and then robotically presented to an optical image scanner for identifying the brightest fragments and classifying the same, with comparison with reference image fragment locations of normal or variant fragments, and for computer storing all relevant data.

The present invention, among other things, provides technologies for detecting and/or quantifying nucleic acids in cells, tissues, organs or organisms. In some embodiments, through sequential barcoding, the present invention provides methods for high-throughput profiling of a large number of targets, such as transcripts and/or DNA loci.

Improved systems and methods for performing genetic analyses. Full genomic DNA scans are performed on the genetic DNA from a plurality of individuals to identify genetic variants. For those variants, but not based on a full genetic DNA scan, the variants alone are scanned in additional individuals to identify blocks of the variants that tend to be inherited together.

The present invention relates to the cloning and high level expression of novel cellulase proteins or derivatives thereof in the in a host cell. Further aspects of the present invention relate to transformants that express the novel cellulases, and expression vectors comprising the DNA gene fragments or variants thereof that code for the novel cellulases derived from Actinomycete using genetic engineering techniques. The present invention is also directed to novel cellulase compositions and methods of use therefore in industrial processes. In particular, the present invention is related to treating textiles with a novel cellulase derived from Actinomycete spp. The present invention also relates to the use of cellulase derived from Actinomycete spp. to enhance the digestibility of animal feed, in detergents, in the treatment of pulp and paper and in the production of starch and treatment of by-products thereof.

The invention discloses a primer composition for discriminating a green-shelled-egg chicken genotype and a use thereof. The primer composition comprises two primer pairs, one primer pair comprises SLCO1B3 gene inserted EAV-HP sequence primers respectively shown in the formulas of SEQ ID NO. 1 and 2, and the other primer pair comprises SLCO1B3 gene non-inserted sequence primers respectively shown in the formulas of SEQ ID NO. 1 and 3. A DNA genome of a chicken to be detected is used as a template and is subjected to PCR amplification under the action of the primer pairs so that a PCR amplification product is obtained. Through detection of the amplified 209bp and 312bp bands, it is determined if the chicken to be detected can produce green-shelled eggs. The primer composition can discriminate the green-shelled-egg chicken genotype.

The invention provides a fluorescence-labeled composite amplification kit for Y chromosome STR (short tandem repeat) gene loci capable of improving distinguishing capability. When the kit is used for detecting DNA (deoxyribonucleic acid) gene, not only can 17 STR gene loci of DYS391, DYS389I/II, DYS439, DYS438, DYS456, DYS458, DYS437, DYS635, DYS448, Y-GATA-H4, DYS19, DYS392, DYS393, DYS390 and DYS385a/b, which can be analyzed by the commercial kit, be amplified and analyzed, but also at least one of STR gene loci of DYS449, DYS527a/b, DYS522, DYS388, DYS447 and DYS444 can be simultaneously amplified and analyzed, so that the accumulative individual distinguishing capability and cumulative probability of exclusion of the system are improved, and the individual distinguishing capability is improved overall.

A method of target DNA genome analysis is provided. The method comprises the steps of: —obtaining non-overlapping segments of target DNA stretches with segment boundaries defined by the presence of particular restriction enzyme recognition sites, whereby the assembly of said non-overlapping segments compose a reduced representation library of said target DNA genome; —obtaining for said segments, raw metrics from a sequencing process applied on said reduced representation library; —clustering non-overlapping, nearby segments with similar raw metrics to provide master segments; —providing metrics describing the master segments, —making a final discrete DNA call based on the master segments and its metrics.

The invention provides an animal product quality safety supervision tracing system. Based on a radio frequency identification (RFID) chip technology, a DNA gene identification technology and a wireless network monitoring technology, the production environment, production, processing, circulation and sale of animal products are monitored in real time around four principal lines of 'production, monitoring, detection and supervision' by using the links of production environment, production, processing, circulation and market entry of the animal products and the like as footings. The animal product quality safety supervision tracing system consists of tracing system equipment and video monitoring system equipment; and the tracing system equipment comprises an RFID chip (ID card or IC card) mark, a DNA gene identification mark, an information data acquisition device, a wireless internet computer (wireless internet personal digital assistant (PDA)), a wireless router and a tracing management software system. The video monitoring system equipment comprises a network camera, monitoring software and a high-capacity storage hard disk.

The invention discloses a method for extracting genome DNA of poultry, applied to large-scale collection, separation and purification of genome DNA of poultry in the actual production. Based on the condition that the poultry blood corpuscle contains a karyon, the method for extracting genome DNA of poultry in the invention proposes a principal that the DNA genome can be extracted once the poultry blood is taken out, completely saving the steps of anticoagulation and blood preservation; the method also proposes that blood is taken from the plantar vein, realizing simple and efficient blood sample collection and less stimulation to the poultry, and furthest avoiding reduction of egg and meal yield because of blood taking. The blood sample not more than 20mul in each poultry is taken on spot and then directly put into a 1.5ml centrifugal tube and mixed with the 700mul of poultry blood lysate in the centrifugal tube, put into an ice-cube box and taken back to the laboratory for DNA extraction; the whole process is secure and reliable, and biological security danger caused by massive poultry blood transport is reduced. Aiming at the problem that protein pollution easily occurs in the poultry blood extraction process, the method of the invention proposes that the DNA is extracted according to the original phenol method, but the saturated phenol is treated for two times and centrifuged at four degrees, thus the protein pollution is extremely reduced, the purification of the extracted DNA is much improved, and the subsequent molecular detection is much facilitated.

The invention discloses a preparation method of gene tracing and anti-counterfeit nanoparticles based on DNA and nanoparticles. The method includes the steps of: a) dispersing a nano-material in a solvent, adding TMAPS and carrying out reaction for 1-24h to obtain surface positively charged nanoparticles; b) adding DNA and carrying out reaction for 24h to obtain DNA modified nanoparticles, whereinthe DNA is a DNA gene fragment longer than 20 base pairs; c) adding TMAPS and TEOS and carrying out reaction for 1-21d to form an SiO2 shell layer on the surfaces of the DNA modified nanoparticles, thus obtaining SiO2 coated nanoparticles; and d) riveting APTES to the surfaces of nanoparticles carrying genetic information so as to obtain gene tracing and anti-counterfeit nanoparticles. The methodprovided by the invention has low operation temperature, the product can exist in a temperature ranging from room temperature to 175DEG C, also can be dispersed in an aqueous phase or oil phase, hasthe advantages of high gene detection sensitivity, no pollution, good stability, high detection precision, multi-point continuous monitoring in a wide range and the like, and can be widely applied inanti-counterfeiting and tracing and other fields.

An artificial hybrid lingzhi was created by protoplast fusion between a cultivar DEG C, and was characterized by unique DNA fingerprints by random amplified polymorphic DNA markers using arbitrary primers: PP4L, PS3L, PS1L, POM and UK, and sequences of nuclear ribosomal DNA gene and mitochondrial ribosomal DNA gene different from those of the parental cultivar.

The invention relates to a penaeus japonicus molecule marking method and application. The penaeus japonicus molecule marking method comprises the steps of extracting a DNA genome, performing PCR amplification and product identification and is applied to diversity analysis of screened penaeus japonicus germplasm resources, genetic diversity analysis, parentage assignment, genetic research of molecular population, genetic map establishment, important economic character positioning, functional gene research and assisting of penaeus japonicus molecule genetic breeding or cultivation. By means of the penaeus japonicus molecule marking method, a genetic variation polymorphic map of a genetic marker gene locus of the penaeus japonicus can be rapidly obtained, the method is simple, convenient and quick, and a result can be visually observed. The penaeus japonicus molecule marking method is mainly applied to genetic marking, genealogical identification and genetic map establishment of a penaeus japonicus colony and is rapid in detection, low in cost and wider in application range.

The invention discloses a method for preparing an exogenous circular RNA and a method for quantitatively determining an endogenous circular RNA. The method for preparing an exogenous circular RNA comprises the following steps: cloning and multiplying an artificially synthesized DAN gene, transcribing the DAN gene into a linear RNA, modifying the tail end of the RNA, and connecting the head and the tail of the RNA to obtain the exogenous circular RNA. Exogenous circular RNA molecules are added to a total RNA according to a specific proportion to serve as a reference gene for real-time quantitative determination of the RNA, and therefore the real-time quantitative determination of the endogenous circular RNA is realized for the first time.

The present invention relates to HBV DNA gene subtype detecting method and kit. Specific probe and matched primer or specific primer and matched reverse primer and specific virus detecting probe are designed based on HBV genotype characteristic sites for detecting HBV genotype alone or performing real-time fluoroscopic examination on the identical sample in several PCR tubes to detect type B and type C or general type. Through the detection, Ct difference is detected and the middle virus content in clinical HBV sample is judged. The kit of the present invention has stable performance, simple operation, fast detection and capacity of fast judging HBV DNA type, and is used in the HBV antagonizing treatment and prognosis analysis.

The invention relates to a detection primer, a detection kit and a detection method of Leber's hereditary optic neuropathy (LHON) mitochondrial DNA gene mutations. The invention, on the basis of Sanger sequencing principle, has the characteristics of being highly sensitive, stable and accurate; four most common pathogenic mutations of LHON mtDNA can be simultaneously detected, so that the problems of an existing LHON mitochondrial DNA mutation detection method, which is low in sensitivity, not strong enough in specificity, capable of causing pollution easily, high in expense and the like, can be solved; therefore, an accurate gene diagnosis method is provided for LHON; and the invention has important implications for genetic counseling and gene therapy of the disease (the LHON).

A method of detecting in an individual an increased risk of hyperplastic vasculomyopathy, or a vascular disease resulting therefrom is disclosed. The method comprises obtaining a DNA genome sample from the individual and detecting in the sample a missense mutation in a gene which is a component of a smooth muscle cell contractile unit. In some embodiments the gene is ACTA2 and in some embodiments the gene is MYH11. In some embodiments, the gene is sequenced and then compared to a panel of control gene sequences which are representative of the same gene in individuals without vascular disease or who are at low risk of developing hyperplastic vasculomyopathy, to detect any missense mutations in the gene. The presence of a missense mutation in the gene indicates an increased risk of hyperplastic vasculomyopathy.

The invention relates to a method for centralized expanding and detecting the gene region of a genome DNA, which aims to verify the characteristics of the gene area in the genome. The characteristics comprise single nucleotide polymorphism (SNP), point mutuation, sequence insertion/deletion and the levels of dideoxy nucleotide (DNA) methylated CpG sites. With an Alu family, particularly an AluY subfamily common sequence as the main oligonucleotide primer, the method expands the DNA and copies all gene regions which are mostly concentrated in the genome with the oligonucleotide primer inter-alu PCR expanded genome DNA. The method is characterized in that the inter-Alu PCR can filter some non-gene sequences, so that a novel generation of sequencing technology detects the SNP, the point mutuation, the sequence insertion/deletion and the levels of DNA methylated CpG sites of the gene region in a centralized way, so as to save the consumption of the genome DNA required by the sequencing.

The invention relates to an adeno-associated virus (AAV) producer cell comprising nucleic acid sequences encoding: rep/cap gene; helper virus genes; and the DNA genome of the AAV vector particle, wherein the nucleic acid sequences are all integrated together at a single locus within the AAV producer cell genome. The invention also relates to nucleic acid vectors comprising a non-mammalian origin of replication and the ability to hold at least 25 kilobases (kb) of DNA, characterized in that the nucleic acid vectors comprise nucleic acid sequences encoding: rep/cap gene, and helper virus genes. The invention also relates to uses and methods using the nucleic acid vectors in order to produce stable AAV packaging and producer cell lines.

The invention provides a gene synthesis method. The method comprises the steps that 1, a to-be-synthesized gene sequence is divided into a first continuous fragment with the length being 55-100 bp, the first continuous fragment is designed to be a forward primer, a gene sequence staggered with the first continuous fragment at the portion of the length of 15-20 bp serves as a second continuous fragment, and continuous reverse primers are designed on a reverse complementary sequence of the second continuous fragment; 2, the forward primer is mixed with the reverse primers; 3, the primer mixture is boiled, annealing is conducted, T4DNA ligase is added, and the primers are connected into a double-stranded DNA gene with the two ends provided with the long sticky tail ends; 4, the synthesized double-stranded DNA gene is connected to a pUC57-GFP-Amp carrier, the sequence of the pUC57-GFP-Amp carrier is shown as a sequence table 1, escherichia coli cells are converted, a non-fluorescing bacterial colony is reversely screened, and sequencing verification is conducted. Accordingly, the method does not reply on PCR amplification, and the defects that the experimental procedure in PCR amplification is tedious, and the PCR technical error rate is high are overcome.