Penaeus japonicus molecule marking method and application

A technology of molecular markers and prawns, which is applied in the field of DNA molecular genetic markers in molecular biology, can solve the problems of time-consuming and complicated markers, and achieve the effects of wide application, rapid detection, and simple and fast methods

Inactive Publication Date: 2015-10-28
ZHEJIANG MARINE DEV RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a convenient and fast molecular marker method for Penaeus japonicus in order to solve the existing defects of identifying different markers of different Penaeus japonicus families, which are complex and time-consuming

Method used

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  • Penaeus japonicus molecule marking method and application
  • Penaeus japonicus molecule marking method and application
  • Penaeus japonicus molecule marking method and application

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Experimental program
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Effect test

Embodiment 1

[0028] A method for molecular markers of Penaeus japonicus, said method for molecular markers of Penaeus japonicus comprises the following steps:

[0029] 1) DNA genome extraction: Take 150 mg of Penaeus japonicus tissue, cut it into pieces, put it into a 1.5 mL centrifuge tube, add 500 μl of buffer solution (10 mmol / L Tris-HCl, pH 8.0; 50 mmol / L EDTA, pH 8.0), mix well Add SDS at a final concentration of 1% and proteinase K at 200 μg / ml, bathe in water at 55°C for 3 hours; centrifuge at 12,000 rpm for 5 minutes, and take the supernatant; add Tris-saturated phenol:chloroform equal to the volume of the supernatant to the supernatant : Isoamyl alcohol mixed solution extraction once, Tris-saturated phenol: chloroform: isoamyl alcohol volume ratio is 25:24:1, then 12000 rpm / min centrifugal 5min, get supernatant, add supernatant and supernatant to supernatant Liquid equal volume of chloroform: isoamyl alcohol mixed solution was extracted once, the volume ratio of chloroform: isoamy...

Embodiment 2

[0034] A method for molecular markers of Penaeus japonicus, said method for molecular markers of Penaeus japonicus comprises the following steps:

[0035]1) DNA genome extraction: Take 180 mg of Penaeus japonicus tissue, cut it into pieces, put it into a 1.5 mL centrifuge tube, add 500 μl of buffer solution (10 mmol / L Tris-HCl, pH 8.0; 50 mmol / L EDTA, pH 8.0), mix well Add SDS at a final concentration of 1% and proteinase K at 200 μg / ml, bathe in water at 55°C for 3 hours; centrifuge at 12,000 rpm for 5 minutes, and take the supernatant; add Tris-saturated phenol:chloroform equal to the volume of the supernatant to the supernatant : Isoamyl alcohol mixed solution extraction once, Tris-saturated phenol: chloroform: isoamyl alcohol volume ratio is 25:24:1, then 12000 rpm / min centrifugal 5min, get supernatant, add supernatant and supernatant to supernatant Liquid equal volume of chloroform: isoamyl alcohol mixed solution was extracted once, the volume ratio of chloroform: isoamyl...

Embodiment 3

[0040] A method for molecular markers of Penaeus japonicus, said method for molecular markers of Penaeus japonicus comprises the following steps:

[0041] 1) DNA genome extraction: Take 200 mg of Penaeus japonicus tissue, cut it into pieces, put it into a 1.5 mL centrifuge tube, add 500 μl of buffer solution (10 mmol / L Tris-HCl, pH 8.0; 50 mmol / L EDTA, pH 8.0), mix well Add SDS at a final concentration of 1% and proteinase K at 200 μg / ml, bathe in water at 55°C for 3 hours; centrifuge at 12,000 rpm for 5 minutes, and take the supernatant; add Tris-saturated phenol:chloroform equal to the volume of the supernatant to the supernatant : Isoamyl alcohol mixed solution extraction once, Tris-saturated phenol: chloroform: isoamyl alcohol volume ratio is 25:24:1, then 12000 rpm / min centrifugal 5min, get supernatant, add supernatant and supernatant to supernatant Liquid equal volume of chloroform: isoamyl alcohol mixed solution was extracted once, the volume ratio of chloroform: isoamy...

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Abstract

The invention relates to a penaeus japonicus molecule marking method and application. The penaeus japonicus molecule marking method comprises the steps of extracting a DNA genome, performing PCR amplification and product identification and is applied to diversity analysis of screened penaeus japonicus germplasm resources, genetic diversity analysis, parentage assignment, genetic research of molecular population, genetic map establishment, important economic character positioning, functional gene research and assisting of penaeus japonicus molecule genetic breeding or cultivation. By means of the penaeus japonicus molecule marking method, a genetic variation polymorphic map of a genetic marker gene locus of the penaeus japonicus can be rapidly obtained, the method is simple, convenient and quick, and a result can be visually observed. The penaeus japonicus molecule marking method is mainly applied to genetic marking, genealogical identification and genetic map establishment of a penaeus japonicus colony and is rapid in detection, low in cost and wider in application range.

Description

technical field [0001] The invention belongs to DNA molecular genetic marker technology of molecular biology, in particular to a molecular marker method and application of the marine economic animal Penaeus japonicus. Background technique [0002] Penaeus japonicus, also known as flower shrimp, bamboo-jointed shrimp, flowertail shrimp, spotted shrimp, and car shrimp, is widely distributed in the Indian Ocean and the Pacific Ocean, and is also distributed along the south coast of Jiangsu Province, China. Penaeus japonicus has rapid growth, strong disease resistance, long-term immortality out of water, suitable for long-distance dry transportation and live sales, and is an important foreign exchange-earning fishery shrimp. Penaeus japonicus is rich in nutrients, and the protein content is several to dozens of times that of fish, eggs, and milk; it is also rich in minerals such as iodine, potassium, magnesium, and phosphorus, as well as vitamin A, aminophylline, and other ingre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/686C12Q2565/125C12Q2531/113
Inventor 杨会成李瑞雪廖妙飞相兴伟周宇芳
Owner ZHEJIANG MARINE DEV RES INST
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