168 results about "Diversity analysis" patented technology

The invention relates to the multimedia technology, and discloses an audio processing method and a device thereof. The method comprises the following steps of: acquiring a normalized energy diversity ratio of spectrums of left and right sound channels by performing diversity analysis on the spectrums of the left and right sound channels, obtaining an enhanced filter coefficient according to the normalized energy diversity ratio and a sequence for shaping, and performing enhanced filtration on the spectrum of a stereo signal according to the obtained enhanced filter coefficient. Because the enhanced filtration is finished in a frequency domain by using the enhanced filter before a frequency domain signal is converted into au audio signal through a synthesis filter, the audio processing effect can be greatly improved so that the finally output audio has better stereo enhancing effect or voice removing effect.

The invention discloses an automatic method for analyzing constitution and diversity of a bacterial community of a 16SrRNA gene. The automatic method comprises the following steps: in 16SrRNA sequencing data analysis process, by taking original sequencing sequence data as input, calling a standard analysis tool (such as Mothur and QIIME) of the industry, and performing visualization on data finally, thereby obtaining analysis results which are easy to analyze. The automatic method includes popular main analysis items at present, meanwhile analysis contents can be modularized, relatively rich and deep data mining analysis methods can be available, different analysis module contents can be combined according to different demands, and process procedures can be relatively reasonably arranged; and in addition, analysis errors caused by different sequencing depths can be eliminated, and the analysis results can be relatively comprehensive, accurate and reliable.

The invention provides a sextuple PCR (polymerase chain reaction) detection method of a portunus trituberculatus miers microsatellite marker. The detection method comprises the following steps of: designing and synthesizing a sextuple PCR primer; extracting genome DNA (deoxyribonucleic acid); performing sextuple PCR reaction; and detecting a PCR product, wherein the sextuple PCR reaction comprises a sextuple PCR reaction system and a sextuple PCR reaction procedure. The method builds the sextuple PCR reaction system which performs the portunus trituberculatus miers family and paternity test to simultaneously detect six microsatellite sites in the PCR reaction, so that efficiency is improved by about six times compared with the existing PCR method. The method has the characteristics of being high-efficiency, economical, simple, convenient and the like, thereby being capable of being popularized and applied in the portunus trituberculatus miers genetic diversity analysis, the genetic relationship test, the family management and the improved variety breeding.

The invention belongs to the fields of molecular marker technology exploitation and application, and concretely provides a plum SSR labeled primer pair exploited on the basis of a transcriptome sequence, and an application thereof in plum germplasm resource researches. The primer pair is exploited on the basis of the transcriptome sequence, batch treatment of a large amount of sequence information is carried out on the basis of transcriptome sequencing, and large batch exploitation of SSR labels can be realized through SSR site searching and SSR primer designing, so the exploitation efficiency is greatly improved, and the problems of complex steps, large workload and high exploitation cost of traditional SSR label exploitation methods are solved. The validity of SSR primers is verified through SSR primer screening and diversity analysis. Foundation is laid for plum SSR labeling primer exploitation and researches of the plum germplasm resource diversity, the kind identification and the genetic relationship by using the SSR molecular labels.

The invention discloses an EST-SSR marker primer combination, which includes 12 pairs of EST-SSR available primers. A screening method of the EST-SSR marker primer combination comprises the steps of SSR locus screening in an EST sequence obtained through sequencing of a transcriptome of pea, primer design, PCR amplification, capillary electrophoresis detection to a product and marker screening. The EST-SSR marker primer combination is applied in germplasm genetic diversity analysis of dwarf type and sprawl type of peas. The EST-SSR marker primer combination can be used for quickly and accurately distinguishing different germplasm materials of peas and analyzing the genetic relationship, thereby providing a basis of oriented seed breeding of the varieties.

The invention relates to a method for preparing microbe bacteria liquid for treating black-odor riverway. The method comprises the following steps: collecting mud at bottom of the riverway, performing microflora diversity analysis on the mud through a high-flux sequencing experiment, performing primary screening, taking mud at the bottom of the riverway, inoculating the mud into a aseptic nitrogen-removal bacterium enrichment nutrient solution, inoculating the mud into fresh enrichment nutrient solution, detecting the ammonia nitrogen and nitrate nitrogen content in the medium, taking mixing bacteria liquid with less accumulation of nitrogen as the primarily screened flora having nitrogen removal effect; optimizing enrichment screening, inoculating the fresh optimized enrichment medium into the primarily screened nitrogen-removal bacteria liquid, adding a trace element solution in the mixing bacteria liquid, inoculating the mixing bacteria liquid added with the trace element into the liquid medium which takes calcium carbonate and sodium citrate as carbon sources, uniformly preparing an uniform breeding diluents by the mixing bacteria liquid, inoculating the material to beef extract peptone nutrient agar, uniformly distributing the materials in a flat, and culturing the material.

The invention relates to the technical field of plant variety identification and breeding, in particular to a building method of flax SSR fingerprint chromatography. According to the method, through MISA software, a flax genome is searched for to obtain 28751 SSR sites, Primer 5.0 is used for designing 71184 pairs of primers, and 26 flax genetic resources are used for screening out the 11 pairs ofSSR markers rich in polymorphism: RM4-5, RM4-7, RM6-2, RM6-6, RM6-7, RM7-1, RM7-3, RM8-1, RM9-4, RM13-5 and RM15-1. The SSR primers which are developed based on the flax genome have the advantages ofbeing stable in amplification, clear in electrophoretic band, rich in polymorphism and the like, and the building method of the flax SSR fingerprint chromatography can be effectively used for the study fields of flax genetic resource genetic diversity analysis, high-density chromatography building, variety purity and authenticity identification, molecular marker-assisted breeding and the like.

The invention relates to a molecular marker for japonica rice genetic diversity analysis and an authentication method for the molecular marker. The molecular marker comprises 32 pairs of SSR markers covering 12 chromosomes of paddy rice; each of the fourth, sixth and ninth chromosomes has one marker, each of the fifth, seventh, eighth and tenth chromosomes has two markers; each of the second, third and twelfth chromosomes has three markers; and each of the first and eleventh chromosomes has six markers. The authentication method comprises the steps of a, DNA extraction; b, PCR (polymerase chain reaction) amplification: a PCR reaction system is 15 microlitres and contains 50mM of KCl, 10mM of Tris-HCl(pH8.8), 0.1 percent of Triton-X, 1.5mM of MgCl2, 200mu M of each of NTPs, 0.2mu M of each primer, 5 percent (v/v) dimethyl sulfoxide and 0.5UT of aq DNA polymerase; c, PCR product detection; and d, statistical analysis. According to the molecular marker for japonica rice genetic diversity analysis and the authentication method for the molecular marker, the genetic diversity of a japonica rice material to be detected and genetic difference can be detected, and the molecular markers for the japonica rice genetic diversity analysis are screened.

The invention discloses mulberry EST-SSR (simple sequence repeat) molecular markers, and a core primer group and application thereof. A large amount of SSR molecular markers are developed by performing transcriptome sequencing and sequence information process on mulberries, 6 groups of polymorphic markers are screened from the SSR molecular markers, and a technological system according with SSR marker fluorescence detection is established. Through amplification and detection on the DNA of 21 mulberry varieties, the 6 groups of polymorphic markers have rich primer amplification polymorphism and high repeatability, the genetic diversity analysis result is identical to morphological morus plant classification, and the 6 groups of polymorphic markers are new markers existing stably and can be applied to mulberry germplasm resource genetic diversity analysis, variety identification, genetic map construction and molecular marker assistant breeding.

The invention relates to a specific primer system of EST (expressed sequence tag)-SSR (simple sequence repeat) molecular markers for Pleurotus ostreatus and application of the specific primer system, belonging to the technical field of molecular biology. The specific primer system of EST-SSR molecular markers for Pleurotus ostreatus comprises 4 pairs of specific primers for EST-SSR molecular markers. The invention also provides the application of the specific primer system of EST-SSR molecular markers in the genetic diversity analysis and the germplasm resource identification of the Pleurotusostreatus. The specific primer system disclosed by the invention is used for establishing a core germplasm bank of the Pleurotus ostreatus, has convenience for use, can simply and rapidly carry out the genetic diversity analysis of germplasm resources, germplasm resource identification, genetic map construction and research on functional genes; and compared with a traditional method, the invention can greatly shorten an analytical cycle and increase research efficiency.

The invention relates to a penaeus japonicus molecule marking method and application. The penaeus japonicus molecule marking method comprises the steps of extracting a DNA genome, performing PCR amplification and product identification and is applied to diversity analysis of screened penaeus japonicus germplasm resources, genetic diversity analysis, parentage assignment, genetic research of molecular population, genetic map establishment, important economic character positioning, functional gene research and assisting of penaeus japonicus molecule genetic breeding or cultivation. By means of the penaeus japonicus molecule marking method, a genetic variation polymorphic map of a genetic marker gene locus of the penaeus japonicus can be rapidly obtained, the method is simple, convenient and quick, and a result can be visually observed. The penaeus japonicus molecule marking method is mainly applied to genetic marking, genealogical identification and genetic map establishment of a penaeus japonicus colony and is rapid in detection, low in cost and wider in application range.

The fast extraction method of adnascent microbe community total DNA of sponge includes cutting sponge sample into small blocks, suspending on TE buffering liquid and set on ice for direct grinding to cracking sponge tissue, adding lysozyme, sodium dodecyl sulfonate and proteinase K into the cracked sponge, centrifuging and adding Tris-saturated phenol to extract, extraction with Tris-saturated phenol, chloroform and isopentanol solution for several times, adding NaCl and isopropanol to obtain DNA precipitate, washing and dissolving in TE buffering solution, slaking with RNA enzyme, and final agarose gel electrophoresis analysis to detect extracted DNA sample. The said method can obtain total DNA sample of adnascent microbe community in large segment of sponge for the need of adnascent microbe community structure and diversity analysis based on nucleic acid.

The invention discloses a multi-time-sequence intestinal flora data analysis process control method. The analysis process mainly comprises the following stages: sample acquisition, intestinal flora data preprocessing, diversity analysis, (sequential sequence) clustering analysis, correlation analysis, bacterial colony interaction network construction and single strain change trend (sequence) prediction. A user inputs a file and corresponding parameters according to the requirements of a process file, and the system automatically analyzes data and outputs the corresponding file and a visualization result. Scientific researchers, including researchers who do not understand data analysis, can efficiently complete a set of standardized intestinal flora data analysis process based on a time sequence, and a final result is obtained. Therefore, the purposes of improving scientific research work efficiency and reducing scientific research cost are achieved.

The invention provides an ISSR molecular marker method for genetic diversity analysis of hedyotis diffusa, and belongs to the technical field of molecular biology. The method comprises the following steps of selecting four primers with clear background, good amplification effect and high polymorphism, amplifying genomic DNAs of the hedyotis diffusa by using ISSR-PCR reaction, analyzing stripes according to an ISSR-PCR result, establishing a DNA fingerprint of the hedyotis diffusa, and constructing a genetic resource database of the hedyotis diffusa. According to the method of the invention, a new method for identification of variety of the hedyotis diffusa and analysis of genetic diversity is created, so as to effectively solve the problem of poor representativeness of a database in the construction of the genetic marker database in the past, and also solve the problem that the existing marking technology has a defect in the hedyotis diffusa genetic resources; the method has the advantages of high polymorphism, good repeatability, low cost, and simple testing procedure, and is not affected by an environment.

The invention relates to a method for rapidly detecting microsatellite markers of Charybdis feriatus. The method comprises the following steps of: (1) extracting a genomic DNA (Deoxyribose Nucleic Acid) of the Charybdis feriatus; (2) obtaining a gene sequence containing microsatellite repeats according to the functional gene sequence of the Charybdis feriatus in a GeneBank database; (3) designing a microsatellite marker primer; (4) implementing PCR (Polymerase Chain Reaction) amplification on the genomic DNA of different individuals of the Charybdis feriatus; and (5) detecting denaturing polyacrylamide gel electrophoresis of PCR products. The method has the advantages of rapidness, accuracy, sensitivity and the like, and can intuitively detect the genotype of different individuals of the Charybdis feriatus, so that a polymorphism map of genetic variation of the Charybdis feriatus on a functional gene microsatellite site is obtained quickly; the microsatellite markers can be applied to the fields of analysis on genetic variation and population genetic diversity of the Charybdis feriatus.

The invention provides a group of cabbage mustard InDel molecular markers as well as a development method and an application thereof, which relate to the technical field of molecular markers. The cabbage mustard InDel molecular marker provided by the invention comprises primer pairs corresponding to 60 sites, and the nucleotide sequences of the primer pairs are shown as SEQ ID NO. 1 to SEQ ID NO.120. The marker developed by the invention has the characteristics of simple operation, good stability and high polymorphism; the primer pairs are good in stability, are uniformly distributed in 9 linkage groups, can be used for positioning of important agronomic trait genes of cabbage mustard, genetic diversity analysis, fingerprint construction, genome-wide association analysis and genetic linkage map construction or molecular marker-assisted selective breeding, and can improve the working efficiency.

The invention discloses an enteric microorganism detection data analysis method, an automatic interpretation system and a medium. The method comprises the following steps: acquiring enteric microorganism sequencing data of a user; extracting sample data from the sequencing data, and filtering the sample data; performing species annotation classification and function annotation classification on the filtered sequencing data to obtain an annotation result; performing conventional analysis on the annotation result, wherein the conventional analysis comprises diversity analysis and probiotics content and pathogenic bacteria content analysis; and obtaining a flora function and disease association database, and based on the annotation result, automatically interpreting the conventional analysisresult according to the flora function and disease association database. According to the method, analysis and interpretation can be automatically executed, so that the working pressure is reduced, the generated analysis results are diversified in form and good in readability, the requirements of users are met, process and batch operations are conveniently carried out, and the workload of manual interpretation is small, visual and convenient.

The invention discloses an EST-SSR primer group developed on the basis of transcriptome sequences of hemarthria compressa and hemarthria altissima. The EST-SSR primer group comprises 52 pairs of primers, and nucleotide sequences of the primers are represented as sequence tables SEQUENCE ID NO.1-104. The invention further relates to an application of the EST-SSR labeled primers in the aspects of genetic diversity analysis of hemarthria sibirica and transferability of the hemarthria sibirica in gramineae species. The 52 pairs of primers in the EST-SSR primer group developed on the basis of the transcriptome sequences of hemarthria compressa and hemarthria altissima have the advantages of abundant polymorphism, stable amplification, good repeatability, convenience in counting and the like, can be effectively applied to research fields such as hemarthria sibirica germ resource genetic diversity analysis, variety identification, fingerprinting construction, molecular marker assisted breeding and the like and are applied to correlational research of other gramineae forage species.

The invention discloses an SSR primer group developed on the basis of a zucchini transcriptome sequence and application of the SSR primer group in identification of a genetic relationship and a hereditary character of zucchini, and belongs to the technical field of molecular biology. The SSR primer group comprises 35 pairs of primers, and nucleotide sequences of the first pair of primers to the 35th pair of primers are as shown in SEQ ID No. 1 to SEQ ID No. 70. The obtained SSR primer group has the advantages of abundant polymorphism, good repeatability, stable amplification, clear electrophoretic bands and the like. Meanwhile, because the primers are from zucchini expression genes and can reflect variation of a functional domain in a genome, diversity of genetic germplasm of loofah can be distinguished well. The primer group can be used for zucchini material resource genetic diversity analysis, genetic relationship identification, DNA finger-print construction and the like.

The invention discloses a method for obtaining a nucleotide sequence in the V3 region of 16S rRNA genes of bacteria and a special primer thereof. The special primer is a specific primer pair consisting of nucleotides having a sequence 17 and a sequence 19 in a sequence table. The method comprises the following steps of: (1) carrying out PCR amplification by using a universal primer and using the genome DNA of the bacterium as a template, wherein the universal primer is a primer pair consisting of nucleotides having a sequence 17 and a sequence 18 in the sequence table; (2) carrying out PCR amplification by using the specific primer and using the PCR product of the step (1) as a temple; (3) sequencing the PCR product in the step (2) by using a sequencing primer, wherein the nucleotide sequence of the sequencing primer is shown as the sequence 20 in the sequence table. The primer pair aiming at the V3 region of 16S rRNA genes for bacterial diversity analysis provided by the invention can be directly used for sequencing without carriers, which is beneficial to reducing the experimental cost, shortening the experimental period and enhancing the accuracy of the experiment. The specific primer pair and the method are particularly suitable for bacterial diversity test of environmental sample aiming at the V3 region of 16S rRNA genes by using PCR-DGGE (PCR-Denaturing Gradient Gel Electrophoresis) technology.

The invention relates to a diversity analysis method based on a chemical structure with CPU (Central Processing Unit) acceleration. The method comprises the following steps of: (a) reading data in chemical structure linked lists in a query library and an inquired library in memory equipment into a main memory; (b) respectively resolving the data as a tree topology sub map set of chemical environment coding of the query library and the inquired library, and storing the tree topology sub map set in the main memory; (c) respectively comparing the tree topology sub map set of chemical environment coding of the query library and the inquired library with a tree topology sub map template, respectively generating binary data for the query library and the inquired library, and storing the binary data in the main memory; (d) transmitting the binary data in the query library and the inquired library to frame caching from the main memory; (e) reading the binary data in the query library and the inquired library from the frame caching by the CPU, and calculating both similarity value by the CPU; (f) transmitting the similarity value to the main memory from the frame caching; (g) reading the similarity value from the main memory and outputting the similarity value to the memory equipment by the CPU.

The invention discloses SSR (Simple Sequence Repeat) primers of platygaster robiniae buhl and duso and application thereof to population genetic diversity analysis. The invention designs according tomitochondrial genome data of the platygaster robiniae buhl and duso to obtain more than 130 thousand of SSR sequences and screens out 37 pairs of SSR specific primers which are relatively stable and have excellent polymorphism from the SSR sequences. The invention further discloses application of the 37 pairs of SSR specific primers in the aspects of population genetic diversity analysis, population evolution analysis and polymorphic analysis of the platygaster robiniae buhl and duso, construction of fingerprint spectrum of the platygaster robiniae buhl and duso and the like. The SSR primers and the application thereof have the important value for developing natural enemy class group population genetic diversity research of the platygaster robiniae buhl and duso, mastering the source and the population differentiation condition of the platygaster robiniae buhl and duso and the like.

The invention discloses a bare sand land vegetation quick recovery method. The method comprises the first step of selecting seeds, the second step of preparing a tile-type vegetation bag, wherein the second step comprises the procedures of a, conducting mixed matching on multiple plant seeds according to the proportion of the degree of dominance; b, uniformly mixing organic compost and sand according to the volume ratio of 1:3, and adopting the mixture as a seed growing matrix; c; sowing seeds on the growing matrix to prepare the tile-type vegetation bag. Compared with the prior art, through a phytocoenology investigation method, diversity analysis is conducted, seed selection is conducted according to the calculation of the degree of dominance, a natural or secondary growth plant community structure in a controlled region is simulated, according to a plant community natural matching rule, seed configuration is conducted on multiple selected species according to the degree of dominance, the adaptability of the seed in a local environment is guaranteed, the seeds are not prone to death, and the vegetation recovery effect is thus not influenced. By precisely calculating the size of the vegetation bag, the vegetation bag is convenient to deliver and lay, the vegetation bag can be entirely buried by wind and sand within a short time, the water and fertilizer are retained, and the vegetation bag is quickly degraded.