Method for preparing microbe bacteria liquid for treating black-odor riverway

A technology of microbial bacteria liquid and river channel, applied in chemical instruments and methods, biological water/sewage treatment, bacteria, etc., can solve the problems of shrinking water area, low self-purification ability of water body, low feasibility of full coverage of rain and sewage diversion pipe network, etc. , to achieve the effects of stable growth, elimination of black odor in river channels, and rapid improvement of water quality

Inactive Publication Date: 2017-05-17
DANYANG SHANGDE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Coupled with the aging of urban drainage facilities, the feasibility of full coverage of the rain and sewage diversion pipe network is low, and the phenomenon of illegal and disorderly drainage occurs from time to time, causing some sewage to be directly discharged into rivers
In

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1, a kind of microbial bacterium liquid preparation method of controlling black and smelly river course is characterized in that, comprises the following steps:

[0018] (1) Collect river sediments and analyze the diversity of sediment microbial communities through high-throughput sequencing experiments. The high-throughput sequencing process includes sediment DNA extraction, PCR amplification and product purification, and Miseq sequencing;

[0019] (2) Initial screening, take 5~10g of river sediment, inoculate in sterile denitrification bacteria enrichment culture solution, place it in a 30°C incubator for static culture, cultivate for 3~4 days, and insert fresh enrichment culture In the culture medium, expand the culture three times to obtain the preliminary screening of the dominant flora, detect the content of ammonia nitrogen and nitrate nitrogen in the culture medium, and take the mixed bacterial liquid with less nitrogen accumulation as the flora with d...

Embodiment 2

[0024] Embodiment 2, the preparation method of a kind of microbial bacterium liquid of controlling black and odorous river course described in embodiment 1, described nitrogen-removing bacteria enrichment medium is (NH4) SO4 (132) 1g, KNO3 (101) 2g, citric acid Sodium (C6H5Na3O7·2H2O, 294.07) 6g (C / N>5), K2HPO4 1g, magnesium sulfate heptahydrate 0.2g, distilled water 1000ml, sterilize at 121°C for 20min.

Embodiment 3

[0025] Embodiment 3, a kind of microbial preparation that is used for river course water quality restoration described in embodiment 1, the pH value of described trace element solution is 8.

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PUM

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Abstract

The invention relates to a method for preparing microbe bacteria liquid for treating black-odor riverway. The method comprises the following steps: collecting mud at bottom of the riverway, performing microflora diversity analysis on the mud through a high-flux sequencing experiment, performing primary screening, taking mud at the bottom of the riverway, inoculating the mud into a aseptic nitrogen-removal bacterium enrichment nutrient solution, inoculating the mud into fresh enrichment nutrient solution, detecting the ammonia nitrogen and nitrate nitrogen content in the medium, taking mixing bacteria liquid with less accumulation of nitrogen as the primarily screened flora having nitrogen removal effect; optimizing enrichment screening, inoculating the fresh optimized enrichment medium into the primarily screened nitrogen-removal bacteria liquid, adding a trace element solution in the mixing bacteria liquid, inoculating the mixing bacteria liquid added with the trace element into the liquid medium which takes calcium carbonate and sodium citrate as carbon sources, uniformly preparing an uniform breeding diluents by the mixing bacteria liquid, inoculating the material to beef extract peptone nutrient agar, uniformly distributing the materials in a flat, and culturing the material.

Description

technical field [0001] The invention relates to a microbial preparation, in particular to a microbial preparation for river water quality restoration and a preparation method thereof. Background technique [0002] In recent years, with the increasing development and utilization of environmental resources in cities, a large number of pollutants containing nitrogen and phosphorus nutrients have been continuously discharged into rivers in cities, making river eutrophication increasingly serious, and some river sections even turn black and smelly , seriously affecting the life and health of the surrounding residents. [0003] The deterioration of river water quality is mainly due to the discharge of treated water from urban sewage treatment plants. Although through upgrading and upgrading, the drainage of the sewage plant can meet the national first-class A standard, but after the treated water enters the river, it is difficult to reach the surface water Class IV standard from ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34
Inventor 戴志东
Owner DANYANG SHANGDE BIOTECH CO LTD
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